Technician High School

SUMMARY

Cell Biology Technician with **.* years of experience in the biotechnology

industry. Broad scope of experience, including research, process

development and quality functions. Extensive mammalian cell culture and

protein production with robotic automation, coupled with strong

organizational skills, writing skills, attention to detail and a dedicated

work ethic.

TECHNICAL SKILLS

Mammalian Cell Culture (50+ cell Western Assays/Blotting

lines) Automation (Cell Culture Robots)

Mammalian Cell Line Development for small/large scale

Cell Banking (cGMP) production

Mammalian Process Development Experimental design to improve

Disposable Bioreactors (1-200L rapid production timelines from

scale) vial-to-final harvest

Roller Bottle/Cell Stack/

Hyperflasks

Clarification and Bulk

Filtration

PROFESSIONAL EXPERIENCE

Clarity Qualitative Market Research (ClarityQMR.com)

2012

Consultant

Mammalian cell scale up/cell proliferation in conventional roller bottle

flasks for research

Advised on methods for growing difficult-to-adhere cells (CHO and cancer

cells) in conventional roller bottles

Recommendations on inoculation density, rpm speed for rollers, temperature

adjustments, and maintaining high viability

Input on reliable vendors that produce improved roller bottle flasks

Advised on aseptic technique and optimal harvest cycle times of conditioned

medium using roller bottles

Amgen, Inc. Thousand Oaks, CA 1993 - 2011

Sr. Research Associate, Protein Sciences (2005 - 2011)

Small/Large scale biological production support of Amgen research and

development

Produced proteins, antibodies, cell pellet paste, and other cell mass for

internal/external customers (Immunology, Neurobiology, Protein Chemistry

and other research groups)

Measured protein expression by Western SDS-page gels and ForteBio

fluorescent analyzer

Equipment owner and operator of TAP Cellmate Robot for conditioned media

production utilizing roller bottle vessels with adherent cell lines

Supported research with 2-50L conditioned media productions from Hybridoma

cell lines

Maintained database and back up cell bank for all project cell lines

Performed data analysis, interpretation, record keeping and communication

with collaborators

Supported 100L production runs on 200L programmable Wave reactor

Operated perfusion cultures in disposable Wave reactors with 293 cells

Implemented more effective scale up method by identifying and testing new

cell culture technology. Introduced the Corning Hyperflask tissue culture

flask which saved time, space, and increased productivity

Safety Rep for Mammalian Research Support Group

Research Associate, Protein Sciences (2001-2005)

Supported xenograft model studies for Oncology department through scale up

efforts of over 50 various types of human cancer cell lines. Involved

coordinating study timelines with projected cell growth to meet the needs

and specifications of the study

Implemented use of Corning ESRBs for protein production which improved

titers and reduced roller bottle use

Implemented use of Wave disposable reactors for suspension cell lines to

improve productivity

Contributor on B7RP-1 publication (Potent activity of soluble B7RP-1-Fc in

therapy of murine tumors in syngeneic hosts. International Journal of

Cancer, 103: 501-507. doi: 10.1002/ijc.10831) Feb 10, 2003

Laboratory Technician II, Cellular Process Development (1993-2001)

Cultured CEF (Chicken Embryo Fibroblast) in roller bottles

Prepared growth media, production media and feed media

Contributed to development of early generation serum-free growth medias for

CHO cells

Production of protein/Mab in stainless steel bioreactors

Experience with SF-9 and Hi-5 insect cells for baclovirus program,

including comparison of growth and production media from various vendors

3M Photodyne, Inc. Camarillo, CA 1984 - 1992

Fiber Optic Technician

Produced, assembled, repaired and calibrated meters for telecommunications

companies

Los Robles Hospital, Thousand Oaks, CA 1977 - 1981

Medical Lab Assistant/Phlebotomist

Processed and analyzed patient sera samples in lab. Assisted in autopsies

Phlebotomist

Assisted in histology and pathology as needed

EDUCATION

Future Technical Institute, Westlake Village

- Electronics Computer Technician Certificate

University of California, Los Angeles

Santa Monica High School

REFERENCES

References available upon request

Contact this candidate