610-***-**** (Home) **** Devonshire Road 484-***-**** (Cellular)

acbtdi@r.postjobfree.com Bethlehem, PA 18020 acbtdi@r.postjobfree.com

PROFESSIONAL SUMMARY

Microbiology technician for food testing with 4 years of hands-on

experience. Seeking for a carreer in the food processing facility and I

would provide the best of my working skills for that company to ensure the

highest quality for the products. Demonstrate to work effectively to all

levels of employees with excellent communication, interpersonal, and

organizational skills. Ability to work in a fast paced environment while

handling multiple tasks.

CAREER SUMMARY PERSONAL SUMMARY

1 year of working in Pennsylvania Team-player, works well with all

Department of Agriculture. cultures

1 year of working in private Dedicated and motivated.

company. Assertive and decisive.

4 years of working in food testing Hardwork and paying attention to

facility. details.

3 years of doing research experience Able to travel extensively.

at Wilkes University.

PROFESSIONAL EXPERIENCE

Pennsylvania Department of Agriculture - Harrisburg, PA (2011- 2012)

Laboratory Assistant

Working in the plant pathology department in BioSafety Level 2 on tomato

and pepper plants.

Accomplish the control of spreading of Xanthomonas species infecting tomato

and pepper plants.

Xanthomonas species were performed on amylolytic, pectolytic and carbon

source utilization.

Working in the greenhouse to take care of the tomato and pepper plants and

then perform inoculation of the Xanthomonas species onto the plants.

Gram staining, Gram positive and Gram negative.

Following GMP and SOPs.

Involved in the SOD (sudden oak death) project working with phytophthora

ramorum.

Isolate Xanthomonas species from pepper and tomato leaves and then perform

purifications of Xanthomonas so that we could store them at -80 C.

Responsible for the preparation of media such as nutrient agar, nutrient

with 1% starch, sucrose peptone agar, and sucrose peptone agar with copper

sulfate, Tween medium, yeast dextrose calcium carbonate, and nutrient

broth.

CVIP (Cryogenics Vessels Instrumentation & Piping) - Emmaus, PA (2010-

2011)

Inventory Specialist

Retrieving special ordered parts when the welders request for the projects.

Maintained adequate supplies in the stock room for the welders as well as

ordering special parts and driving fork lifts.

Managed the shipping and receiving department in the company.

Assembled the parts for the welders to weld on the cryogenic vessels.

Entering raw data into the excel program to keep track of the ordered

parts.

Silliker Laboratory - Allentown, PA (2006- 2010)

Microbiology Technician

Screen for presence of E. coli in different food products for the safety of

the consumers.

Test cell cultures to identify the presence of harmful bacteria from food

products.

Perform dilution series for the trace of toxin levels if the harmful

bacteria are existence.

Read plates and tubes, as required, to determine the number of organisms in

the sample. Record findings on worksheet for verification.

Write identification onto plates so that client and sample number can be

tracked.

Pipette sample solution into tubes or plates according to the analysis

process being performed. Pour the agar into plates or tubes to begin the

growth process.

Prepare sample for analysis by recording, weighing, and blending.

Collect plates and place them in the incubator for the specified amount of

time and temperature.

Prepare media and broth for use in pathogen testing as required.

Sterilize materials needed for analysis. Autoclave plates before

discarding.

Perform food testing, pH and other analysis tests on sample when requested

by the client.

Ensure that media and materials are prepared for the next testing sequence.

RESEARCH EXPERIENCE

Wilkes University August 2003 to May 2006

Performed molecular cloning techniques such as DNA purification and PCR.

Measured the respiration, lipid composition, and thermal properties of

Arabidopsis mutants.

Studied the lipid analysis of red and white oak acorn shells.

Determined parent-offspring distances in oak forests by DNA

fingerprinting.

EDUCATION

Wilkes University, PA Bachelor of

Molecular Biology & Chemistry Science 2006

GPA: 3.5/4.0

Cellular Molecular Courses, Biochemistry, Medical Microbiology, Genetics,

Medical Anatomy and Physiology, Bioinformatics, Math Statistics,

Independent Research, Invertebrate Biology, Histology, Organic Chemistry,

SKILLS/KNOWLEDGE AREAS

Real time PCR Highly experienced in aseptic

TLC plate techniques

HPLC Cell growth

SDS-PAGE Read plates and tubes

DNA extraction Identify harmful bacteria

Gel electrophoresis Making agars

Southern blotting Pipetting techniques

Western blotting DNA isolation

Laboratory GMP Know how to use the incubators

Follow SOPs USDA regulations

GC FDA regulations

Cell culture Dilution series

DNA analysis Melting point

Gas chromatography Fatty acid analysis profile

amylolytic Gram staining

Immunoassay Pectolytic

Mouse heart muscle cells

Awards/Honors:

Beta Beta Beta Biological Honors Society (2004-2006)

Honors Award (2002-2006)

Dean's List (2002-2004)

REFERENCES:

Dr. Kim (Pennsylvania Department of Agriculture) 717-***-****

Dr. Terzaghi (Professor at Wilkes University) 570-***-****

Dr. Klemow (Professor at Wilkes University) 570-***-****

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