Quality Control Assistant
Resume:
SNEHA SREE YELETI
************@*****.********.***
To use my training and knowledge from my recently acquired Masters degree and demonstrate my strong organizational and
interpersonal skills. I am a highly determined worker who will strive to better the company in which I work.
Article I. CORE COMPETENCIES
Cell culture maintenance q-PCR
Toxicological and dose response studies RNA isolation
SDS- PAGE Western Blotting
High Performance Liquid Chromatography Cell signaling studies
EDUCATION .
Masters of Science in Pharmaceutical Sciences
Campbell University, Buies Creek, NC. December 2013
Master’s Research Project:
“Characterization of the physiological response and toxicological sensitivity of TAMH (TGF- Alpha Transgenic
Mouse Hepatocytes) cells towards apoptotic drugs in different environments.”
The objective of the study was to monitor for differences in cell morphology, cell viability, toxicological sensitivity and
enzyme expression of hepatocytes cultured using three different methods- non-coated, thin collagen coated, and
sandwich collagen coated plates.
“Developing a purity indicating method for quinine using reverse phase HPLC”
The objective was to develop a method using HPLC to separate the quinine from the degraded quinine sulphate and
quinine hydrochloride samples.
Bachelor’s of Pharmacy
Osmania University, Hyderabad, AP, India. May 2011
Bachelor’s Research Project
“Development of Colon-Targeted Drug Delivery System for Tinidazole using Guar Gum, and Xanthan Gum
Polysaccharides.”
The objective was to study the dissolution and disintegration properties of tablets formulated with either of guar gum and
xanthan gum in simulated gastrointestinal fluids. The poster presentation was ranked first in - Novel Drug Delivery System
at an All India Conference National Institute of Technology-Warangal 2010.
LABORATORY SKILLS AND EXPERIENCE .
Biotechnology skills: Cell culture, bicinchoninic acid assay, Bradford total protein Assay, SDS-PAGE, colorimetric assays,
Western Blot Analysis, Enzyme-linked immunosorbent assay (ELISA), Confocal microscopy, radioactive ligand- receptor binding
assay, RNA isolation, qPCR, RNA isolation, viability assays, animal behavioral assays, primer design, phase contrast
microscopy and cytotoxicity assays- MTT assay, LDH assay, DAPI, and alamar blue assay .
Analytical Instrumentation: HPLC, UV- visible Spectroscopy, gas chromatography, mass spectrometry, Fluorescence
spectroscopy, and thermo gravimetric analyses.
Pharmaceutical Skills: Production and quality control tests according to IP monographs for tablets, capsules, emulsions,
suspensions, lotions, suppositories, elixirs, gels, ointments, nasal solutions, and ophthalmic drops.
SNEHA SREE YELETI
************@*****.********.***
Laboratory experience:
Performed collagen thin coated hepatocytes culture, sandwich coated hepatocyte culture.
Performed cell viability assays like LDH assay and MTT assays on AML-12 cells, TAMH and C2C12 cells.
Conducted isolation and quantification of RNA and performed q-PCR studies for CYP1A1, CYP2B10, and albumin in
hepatocytes.
Performed toxicological studies and cumulative dose response studies by using doxorubicin on hepatocytes and on
isolated rat right atrium and vasdeferens by using beta agonists.
Participated in cell signaling studies to study the effect of fetal bovine serum on intracellular calcium mobilization
through receptor tyrosine kinase signaling pathway in rat aortic muscle cells by using confocal microscopy and Olympus
Fluoview software.
Tail latency test, spontaneous activity test, and rectal temperature in female beta arrestin-2 knockout mice versus wild
mice to study the genotype effect on the sensitivity of tetrahydrocannabinol
Radioactive receptor binding assay to study cannabinoid 1 receptor density and receptor affinity towards specific
ligands
Conducted ligand efficacy studies to monitor the activated G protein through radiolabel GTP.
Performed force degradation studies by exposing acidic, neutral, basic samples of quinine hydrochloride and quinine
sulphate to elevated temperatures, UV & visible light, and hydrogen peroxide.
Performed HPLC to develop a method for increasing resolution of the quinine among the generated impurities.
Experience in production identification of several pharmaceutical drugs using FTIR.
WORK EXPERIENCE .
CAMPBELL UNIVERSITY
Laboratory Teaching Assistant August 2013 - December 2013
Teaching lab practical for basic biology for biology majors
Tutor, Academic support: August 2012 - December 2013
Mentoring undergraduate students in biopharmaceutics, and pharmacokinetics
PERC (Pharmaceutical Education and Research Center) summer - 2012
Maintained chemical inventory and assisted workers in HPLC, FTIR and TGA.
Graduate assistant January 2012- December 2013
Assisted professor in maintaining data for course curriculum meetings, answer papers for undergrad exams.
Wiggins Memorial Library – Student Worker:
Helped students and visitors, and maintained books inventory.
LIFE LINE FORMULATIONS AP INDIA
May -June 2010
Internship:
Participated in manufacturing of tablets, capsules, multiple emulsions, suspensions and solutions.
Performed quality control tests for tablets: weight variation test, friability test, tablet breaking force test,
disintegration test and dissolution test.
Performed light obscuration particle count test and microscopic particle count test for injections.
SYSTEMS/APPLICATIONS :
Graph-Pad prism Fluoview viewer MS Excel HPLC Agilent software
MS Word MS PowerPoint JAMP DOE
SNEHA SREE YELETI
************@*****.********.***
PROFESSIONAL MEMBERSHIPS .
ISPE February 2012- present
AAPS (Student-Chapter) September 2011-Present
Contact this candidate