Development Research
Resume:
RONALD A. MILLER
Glen Mills, PA *9342
*************@*******.***
EDUCATION
Lehigh University Lehigh, PA
Master of Science, Molecular Biology, January 2010
Thesis: Melatonin Provides a Possible Therapeutic Link Between Circadian Rhythm Deregulation and
Alzheimer’s Like Dementia”; Advisor: Dr. Vassie Ware, Department of Biological Sciences
Professional Development courses completed in Analytical Chemistry and Pharmacology
Wayne State University Detroit, MI
Bachelor of Science with a concentration on Biophysics and Molecular Biology, May 1994
Minor in Chemistry
Recipient of the Summer Undergraduate Research Fellow, WSU School of Medicine (Physiology), 1992
RESEARCH EXPERIENCE
Merck & Company, Inc. West Point, PA
Associate Principal Scientist, Biomarkers April 2010-present
Target validation through the development of biomarker assays for pharmacodynamics applications,
primarily using LC/MS/MS platforms (Waters NanoAcquity systems coupled to Thermo Vantage,
Quantum and Velos-Orbitrap instruments).
Assay development in support of preclinical programs.
Over twelve years’ experience in proteomics-based analysis involving protein sample preparations,
specializing in chromatography, immune-precipitation, fractionation and purification.
Performed automated sample preparation (Hamilton).
Extensive experience in statistical analysis, bioinformatics and data mining.
Senior Research Biochemist, Proteomics and Imaging: Neuroscience 2006-2010
Drug discovery experience involves proteomics-based research in the areas of dementia, sleep disorders,
schizophrenia, and pain research as well as infectious disease (HIV protease inhibitor) adverse effect
characterization.
Research Biochemist, Biological Sciences: Alzheimer’s Disease Research 2000-2006
Supported proteomics-based drug discovery research focused on Alzheimer’s Disease biomarkers .
Staff Chemist, Pre-Clinical Drug Metabolism 1998-2000
GLP-compliant drug metabolism screening for in vitro and in vivo pharmacokinetic studies.
Chemist, Preclinical Drug Metabolism Dec 1996-1998
GLP-compliant drug metabolism screening for in vitro and in vivo pharmacokinetic studies.
Parke-Davis Pharmaceuticals Ann Arbor, MI
Temporary Associate Scientist, Preclinical Drug Metabolism 1994-1996
Drug metabolism research, primarily investigating cross-species metabolites of atorvastatin (Lipitor)
using various LC/MS and LC/MS/MS platforms.
ANR Pipeline Company Dearborn, MI
Gas Analyst (Co-operative) 1993-1994
Crude oil volatile gas composition analysis using GC/MS platforms.
Wayne State University School of Medicine Detroit, MI
Student Intern, Department of Physiology 1993-1995
Student research in neurotransmitter delivery biophysics involving membrane preparations and vesicle
delivery protocols. Recipient of the Summer Undergraduate Research Fund, 1994.
INSTRUMENTATION
Mass Spectrometers: Thermo – TSQ-Vantage, TSQ-Quantum, LTQ-Velos-Orbitrap, LTQ-Orbitrap; ABI-
Sciex – API-5000, API-4500, API-3000; Bruker – Voyager-DE TOF-MS
Nano-LC/HPLC Systems: Waters, Eksigent, Agilent, Perkin-Elmer; GE ÄKTA FPLC
Automated Sample Preparation: Hamilton STARlet Liquid Handler, Biotage RapidTrace SPE
Workstations
SKILLS AND CERTIFICATIONS
Proteomic, biomarker and small molecule research, experimental design and laboratory techniques.
Protein biochemistry platforms and applications.
Sample preparations using immune-capture, solid phase extraction and fractionation methods.
Statistical analysis, data mining and bioinformatics experience.
Excellent collaborator and team-oriented.
Rapid Go/No Go decision making.
Systematic troubleshooting.
Workflow efficiency assessment.
Early Development Course, March 2013.
Six Sigma Yellow Belt Training, February 2013.
CPR and AED Certification, May 2013.
Safety Committee volunteer officer, 2010-present.
Excellent communication skills, written and oral.
Blue Sky Initiative Award, Merck Research Laboratories 2008
Alzheimer’s Accomplishment Award, Merck Research Laboratories 2006
PUBLICATIONS
“Development of a Multiplexed Selected Reaction Monitoring-Mass Spectrometry Based Assay for
Quantitative Targeted Analysis of Post -Translational Modified Tau Protein”, Ronald A. Miller and Daniel
S. Spellman, 2013, In Preparation.
“Quantitative Proteomics in Laser Capture Micro-Dissected Sleep Nuclei from Rat Brain”, Ronald A.
Miller, Christopher J. Winrow, Daniel S. Spellman, Qinghua Song, Duane R. Reiss, James P. Conway,
Francisco J. Dieguez-Acuna, Damon C. Barbacci, Rhonda R. Taylor, Alison M. Rigby, Paul J. Coleman,
Kenneth S. Koblan, John J. Renger and Ronald C. Hendrickson, 2013, submitted for review.
“Pyroglutamate-Modified Amyloid-β Protein Demonstrates Similar Properties in an Alzheimer’s Disease
Familial Mutant Knock-In Mouse and Alzheimer’s Disease Brain”, Guoxin Wu, Ronald A. Miller, Brett
Connolly, Jacob Marcus, John Renger and Mary J. Savage, Neurodegenerative Diseases, 2013, Oct 23,
Epub ahead of print.
“Novel mutations introduced at the ß -site of amyloid precursor protein enhance the production of
amyloid ß peptide by BACE1 in vitro and in cells”, Xiao-Ping Shi, Katherine Tugusheva, James E. Bruce,
Adam Lucka, Elizabeth Chen-Dodson, Binghua Hu, Guo-Xin Wu, Eric Price, Robert, B. Register, Janet
Lineberger, Ron Miller, Mei-Jy Tang, Amy Espeseth, Jason Kahana, Abigail Wolfe, Ming-Chih
Crouthamel, Sethu Sankaranarayanan, Adam Simon, Lin Chen, Ming -Tain Lai, Beth Pietrak, Jillian
DiMuzio, Yueming Li, Min Xu, Qian Huang, Victor Garsky, Mohinder K. Sardana, and Daria J. Hazuda,
Journal of Alzheimer’s Disease, 2005 Apr, 7(2):139-48
"Development and Validation of a High-Performance Liquid Chromatography Tandem Mass
Spectrometry Assay for Atorvastatin, Ortho-Hydroxy Atorvastatin, and Para-Hydroxy Atorvastatin in
Human, Dog, and Rat Plasma", William W. Bullen, Ronald A. Miller and Roger N. Hayes, J. Am. Soc.
Mass Spectrometry, 1999, 10, 55-66.
PRESENTATIONS
“Development of a Targeted LC-MS/MS Assay for the Measurement of Farneyslated Lamin A Peptide:
Assessment of a Potential Adverse Effect Biomarker”, Ronald A. Miller, Qian Huang, Tracy Diamond,
Daniel S. Spellman, Francisco J. Dieguez-Acuña, Mike Miller and Bonnie J. Howell, Presented at the
United States Human Proteome Organization Annual Meeting 2013, Baltimore, MD
“Characterization of Wheat Germ Agglutinin-Enriched Proteins from Non-Human Primate Cerebral
Spinal Fluid”, Ronald A. Miller, Shahriar Niroomand, Jacob Marcus, Joel B. Schachter, Daniel S.
Spellman, and Bonnie J. Howell, Presented at the American Society for Mass Spectrometry Annual
Meeting 2011, Denver, CO
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"Tissue Proteomics: A SILAM-Based Workflow For Targeted Differential Analysis Applied To Sleep
Nuclei", Ronald A. Miller, Christopher J. Winrow, Daniel S. Spellman, Rhonda R. Taylor, Duane R.
Reiss, James P. Conway, Francisco J. Dieguez -Acuña, John J. Renger, and Ronald C. Hendricks on,
Presented at the American Society for Mass Spectrometry Annual Meeting 2009, Philadelphia, PA
"Application of Laser Capture Microdissection Analysis for CNS Studies: A Powerful Tool for Proteomic
Research", Ronald A. Miller, Rhonda Taylor, Alison Rigby, Duane Reiss, Christopher Winrow, John
Renger, Damon Barbacci, Francisco Dieguez and Ronald Hendrickson, Presented at the American Society
for Mass Spectrometry Annual Meeting 2008, Denver, CO
“A Synthetic ß-Amyloid Internal Standard for APP Catabolite Quantitation in Cerebral Spinal Fluid”,
Ronald A. Miller, Presented at the American Society for Mass Spectrometry Annual Meeting 2007,
Indianapolis, IN
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