Data Engineering

Judy Guo

Advisor - Prof. Evan R. Kantrowitz

Chestnut Hill, MA

****@**.*** - 857-***-****

WORK EXPERIENCE

Graduate Student Researcher

Prof. Evan R. Kantrowitz's Lab - Boston, MA - January 2005 to May 2013

Explored the influence of metal on the nucleotide regulation of enzyme.

Performed extensive enzyme kinetic study with different nucleotides in the absence and presence of metals.

Determined the structure of a mutant protein and studied its significance related to the function of the protein

using X-ray crystallography. Work involved protein

expression, purification, crystallization, X-ray data collection and structure

determination of protein. Techniques and methods used include site-directed

mutagenesis, column chromatography, polyacrylamide gel electrophoresis,

hanging drop and microdialysis methods to obtain crystals, X-ray data analysis and structure refinement using

d*TREK, CNS, MOLREP in CCP4 suite, Phenix and COOT.

Utilized virtual high throughput screening to identify potential small molecules as drug candidates for protein.

Computational tools used for the structure based

drug design include AutoDock 3.0, DOCK5, GOLD, Surflex and Xscore.

Developed biochemistry assay to evaluate the inhibition of ligands against protein target.

Wenyue (Judy) Guo Phone: 857-***-**** Email: ****@**.***

Experience with molecular cloning, PCR, agarose gel electrophoresis of DNA,

protein engineering, enzymatic assays, UV/Vis/Fluorescent spectrophotometry.

EDUCATION

Ph.D. in Chemistry

Boston College - Chestnut Hill, MA

September 2004 to August 2012

B.S. in Chemistry

University of Science and Technology of China - Hefei, CN

September 2000 to July 2004

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