Medical Assistant

PERFORMANCE

PROFILE

Resent graduate with strategic experience in clinical research, development of systems, and assay solutions targeting

early disease prevention, diagnosis, treatment, and monitoring. Experience providing medical opinions, support,

guidance, education, and customer support. Provide quality and regulatory compliance with government guidelines.

High level of performance in problem solving, decision-making, team-building skills, and excellent ability to

communicate clearly and accurately.

EDUCATION AND DEGREE CONFERRED

2011-2012 New York Medical College, NY. USA. Pre-Internship Program. Certification June/2012.

2006-2012 Universidad Autonoma de Guadalajara. Mexico. Doctor of Medicine and Surgery.

1999-2004 University of Illinois at Chicago, Chicago, Illinois. USA. Bachelor of Science, Biology.

SKILLS

Fluent in Spanish and English, proficient in Portuguese and Thai

Computer proficient in Windows and Mac OS, Microsoft Office applications, data base management, email,

and social media.

Written communication skills; case reports, publications, proposals, presentations.

PUBLICATIONS

Nuñez R, Garay N, Villafane C, Bruno A, Lindgren V. “Description of flow cytometry approach based on

SYBR-14 staining for assessment of DNA content, cell cycle analysis, and sorting of living normal and

neoplastic cells” Experimental and Molecular Pathology 76 (2004) 29– 36.

Nuñez R, Garay N, Bruno A, Villafane C, Bruno E, Filgueira L. ‘Functional and structural characterization of

two populations of human monocyte-derived dendritic cells’ Exp Mol Pathol. 2004 Oct; 77 (2): 104-15.

MEDICAL ASSISTANT TO CLINICAL DIRECTOR DEP. PEDIATRICS

08/13-10/13

Richmon University Medical Center Dep. Pediatrics, Staten Island, New York.

Provide assistance to the ambulatory pediatrics clinic director to enhance medical services for Staten Island’s low-

income population that includes immigrants from Latin America, Africa, Asia and the Middle East. Translate Spanish

to English for physicians and patients, interview patients and obtain their clinical histories, enter data into

Eclincalworks.Perform physical examinations, assessments, and treatments for pediatric patients. Administer

vaccinations, placement of Foley catheters, blood sample collections and patient counseling. Present cases during

morning rounds and department meetings. Perform physical examinations and evaluations of patients’ evolution, daily

progress, labs and medication orders.

MEDICAL RELIEF MISSION

06/12 – 5/13

Medical Relief Mission/ Out-Patient Clinic Project. Tunja, Boyaca, Colombia.

Provided direct medical care and healthcare education to underserved, rural populations. Due to the

overwhelming need for healthcare in this community I am currently working on co-founding a small clinic for

outpatient care that will be used to visiting physicians doing medical relief work and will collaborate with local

physicians with the goal of providing continuity of care. Additional plans are in effect to develop a project in

cooperation with UNIBOYACA Medical School, to provide education and education to the future local

doctors.

PRE-INTERN

06/11 – 06/12

Richmond University Medical Center. Staten Island, NY. Affiliate of New York Medical College (NYMC)

Worked as a sub-intern in the program sponsored by NYMC in collaboration with the Universidad Autonoma

de Guadalajara, Mexico. During the course of the year I rotated through the departments of Internal Medicine,

Surgery, Ob/Gyn, Pediatrics and Psychiatry at Richmond University Medical Center, Staten Island, NY, an

affiliate hospital of NYMC. IAs an integral member of the team I was able to had same responsibilities as a

first year resident including: presentation of cases in morning rounds, physical examinations and evaluations of

patient's evolution, daily progress note documentation, lab and medication orders and presentation of topics

related to patient care in department meetings. Additionally I assisted in major surgical procedures in the

Department of Surgery (abdominal and orthopedic procedures) and Department of Ob/Gyn (deliveries,

cesarean sections, dilatation and curettage, hysterectomy for benign and malignant conditions, etc.)

RESEARCH RELATED EXPERIENCE

RESEARCH ASSOCIATE

08/04-12/06

Children’s Memorial Hospital Research Center (CMRC), Department of Human Genetics Chicago, IL.

Managed project for the development a mouse model with genotype and phenotype expression of genes

involved in Rett’s Syndrome and Angelman’s Syndrome. The model will be utilized in protocols for

developing genetic modulators for gene expression that could be implemented in therapies to treat or cure

patients with the aforementioned syndromes. Techniques utilized in my project include planning and

assembling plasmid vectors to integrate into the host genome to induce long or short-term gene expression to

increase or decrease protein production in cell culture models. Electroporation and lipofection of cells with

vectors of interest was performed to develop stable cell lines with induced protein expression. To use SiRNA

for candidate gene induction or repression The

effects of modification in expression of Ube3a,

Gabrb3 and Atp10c candidate genes were studied in cell lines by using different induction modulators such as

deoxycycline, G418, neomycin, Zeozin, etc..

RESEARCH ASSISTANT

06/03-08/03

Summer Research Opportunity Scholarship for Minorities.

I was awarded a scholarship and stipend ($3000 plus expenses) based on my academic achievements. The SROP has

several components: First and the most rewarding is the opportunity to work one on one with a University of Illinois

at Chicago (UIC) faculty mentor on a research project. The second component was campus based weekly activities

including workshops, seminars, and symposiums involving all the UIC SROP participants. The third component was

the 2004 CIC/SROP National Conference, which was held from July 9-11, 2004 at the University of Iowa. This

conference included over 500 student participants from the fifteen member CIC institutions, presenting their research

projects and experiences from the program.

RESEARCH ASSISTANT

08/03-08/04

University of Illinois at Chicago, School Of Medicine, Department of Hematology/Oncology, Division of Flow

Cytometry, Chicago, IL.

Managed project for the development of a screening test for early detection of breast cancer based on labeling cell

markers and cytology. Performed tissue culture and maintenance of breast cancer cell lines, cell staining and

karyotyping. Utilized proteomic techniques involving Southern and western blots and various methods of protein

preparation as well as isolation and preparation of DNA and RNA from breast cancer cell lines. Developed protocols

for flow cytometers to assess and sort dendritic cell lines with antibodies (CD34, IgG1, PI, etc.) Flow cytometers

utilized: FACSCaliburk (Becton Dickinson, Heidelberg, Germany), FACStar Plus, and Vantage SE (Becton

Dickinson). Prepared a mixed leukocyte reaction with Carboxin fluoreacetate in tissue samples of leukemia patient for

cell count.

RESEARCH ASSISTANT

04/03-07/03

University of Illinois at Chicago, School Of Medicine, Department of Pharmacology.

Carried out medium preparation and cultures for bacteria (Escherichia coli). Performed protein extraction from

E. Coli. Prepared cleared Lysate buffer under natural conditions as well as other buffers such as: PBS,

lyzome® for lysine E. coli, etc. Executed sonication procedures in E. coli, followed by centrifugation and

filtration of supernatant. Performed batch purification of protein of interest (Glutathione Sepharose 4 Fast

Flow) and examined protein content by SDS-PAGE and western blotting.

VOLUNTEER

10/02-01/03

Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL.

Helped to provide excellence in healthcare by collaborating with physicians and nurses. Transported patients

to various hospital units. Provided support care to patients and assisted with their daily needs.

ASSISTANT TO THE PHYSICIAN

07/01-01/03

Hand Surgery Associates, Chicago, IL.

Performed multiple clinical responsibilities as a medical assistant in an orthopedics group practice including suture

removal, wound care, dressing removal and application and casting. Conducted patient assessment prior to surgery,

maintained patient medical records, supervised medication control records and obtained medical histories. Provided

Spanish to English interpretations between physicians and patients.

REFERENCES

References are available upon request.

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