Medical C
Resume:
SHAMSHER S. SAINI, Ph.D.
**** *** ***** *****, ********* TX 77539
acb88q@r.postjobfree.com
HIGHLIGHTS OF EXPERIENCE
. Experienced in drug discovery (Bristol Myers Squibb Princeton),
Antibodies production, Diagnostic Development, Pre clinical studies,
Authored patents, GLP certified.
. Experience in cloning, sequencing, hyper-expression and purification
of recombinant proteins/antibodies
. Experienced in purification of antibodies, peptides, enzymes,
utilizing filtration, Ammonium sulphate precipitation, ion exchange,
affinity column, sieve chromatography, FPLC and HPLC.
. Protein conjugation/labelling (Invitro and invivo) and
characterization (IP, W blot, ELISA, FACS,
UV/Visual/Fluorescent/Atomic absorption spectroscopy,
Bio/immuno/enzyme assay development, Enzyme kinetics, Cell culture,
Production of antibodies (Monoclonal and Polyclonal) Histopatholgy,
. I am experienced in the area of clinical and experimental autoimmune
Myasthenia Gravis, type-1 Diabetes, inflammatory bowel disease,
Hypertension, Molecular oncology, Rabies, Influenza, E.Coli,
Salmonella and Toxinology
. Skilled in the use of bioinformatics databases, programs & web-tools
and computer applications.
PATENTS & PATENT APPLICATIONS
1. Patent Number: EP1353664 (A2) Heterocycle derivatives and methods of
use for treating anthrax infection. Peterson, J.W., Gessell-Lee, D., and
Saini, S.S. [ 2003-10-22]
2. Patent Number: IN/PCT/2002/01182/DEL. Heterocyclic derivatives and
methods of use. Peterson, J.W., Gessell-Lee, D., and Saini, S.S. [ 2010-
03-26]
3. U.S. Patent application # WO199805640 (Patent pending). Anti-
inflammatory and other therapeutic, prophylactic or diagnostic uses of
synthetic melittin and new related peptides Peterson, J.W., Saini, S.S,
Chopra, A.K., and Wood, T.G. (1997).
4. US Patent application # 200********. Peterson, J.W., Saini, S.S,
Wood, T.G. and Chopra, A.K). PLAP polypeptides and methods of producing
and using same. September 4, 2003
5. U.S. Patent application # 200********. Heterocyclic derivatives and
methods of use. Peterson, J.W., Gessell-Lee, D., and Saini, S.S. (
2002)
WORK EXPERIENCE.
2013: Research Associate ISU Meridian ID
Protein chemistry and Mol Biology: Cloning and Hyper-expression in E
coli and purification of CdFabK (Enoyl Reductase), aiming at
developing inhibitor to Clostridium deficile fatty acid synthesis
2012: Research Technologist, NWLRL, University Washington, Seattle WA
Clinical Immunochem and Mol Biol :
. Analysis of clinical trial samples for IL-6 by ELISA, DNA and RNA
purification, method validation.
2009-2011: Health Science Specialist, Veteran Affair Medical Center
Minneapolis, MN
Molecular Oncology: Managed the lab. Worked on
. Testing several biomolecules as potential therapeutics for
mesothelioma and lung cancer.
. Hyperexpression and purification of recombinant protein eIF4E.
1994-2008: Senior Research Scientist University of Texas Medical Branch,
Galveston, TX.
Myasthenia Gravis [ 2003-2008]
Worked in the area of Protein chemistry, assay developement,
Immunochemistry, Immunobiology Molecular biology of Experimental
Autoimmune Myasthenia Gravis. Managed the lab.Work accomplished:
. Purification of toxin from snake venom and conjugation of toxin with
Sepharose (toxin- affinity column), to purify Torpedo/TE671
Acetylcholine Receptors (AChR) as well as transformation, hyper-
expression and purification of recombinant AChR subunits (his tagged)
to develop myasthenia Gravis in transgenic and gene knockout mice.
. Quantitation of AChR receptor (RIA), Antibody production (ELISA),
Spleenic germinal center (Immunoperoxidase), IgG, MAC Complex deposit
(Immunofluoresent), lymphocyte markers (FACS), Lymph node cells
proliferartion ([3H]TdR uptake)
. Conjugation of subunits with Alexa-488 for cell based assay by flow
cytometery analysis.
. Propogation of hybridoma C1q and M35, and purification of monoclonal
antibodies (Tested by ELISA), used as therapeutic for EAMG
intervention.
. RT-PCR of AChR-alfa subunits mRNA and genotyping of HLA -DQ8, DQ6 and
DR3 by PCR of transgenic mice.
. Cloning of Variable region of Heavy Chain of anti IL-6 rat antibody
from the spleening mRNA of rat immunized with mouse IL-6 to construct
single chain fragment variable (scFv) antibody.
Inflammatory bowel disease, PLA2, Cyclo-oxygenases and Adenylyl
cyclase) [1994-1999, 2001-2002 ]
. Conjugation of PLAP Peptides with KLH to immunize rabbits, screening
sera by ELISA for production of Abs.
. Conjugation of PLAP peptides to epoxy-Sepharose, to purify antibodies
against these peptides
. Conjugation of purified IgG to Sepharose to purify recombinant PLAP
from E coli.
. Immunocytochemical localization of PLAP in the biopsy sample of colon
from the patients with the inflammatory bowel disease, experimental
colitis (DSS model), and small intestine from mice treated with
Salmonella and Cholera toxin.
. Stimulation of PLAP in J774, Raw, CaCO2, HT29 with cholera toxin and
LPS and analyzed by Western blot as well as by autoradiography by
metabolically labeling the cell by tran35S methionine using antibodies
against PLAP and binding of abs to peripheral lymphocytes by FACS
analysis
. Quantitation of phospholipases (A2, C, D) by thin layer
chromatography. Metabolic lebelling of phospholipids with [14C]
Arachidoninc acid. Separation of phospholipids
. Developed a peptide based Maldi-Mass Spectrometery.substrate assay for
anthrax LF.
. Developed assay for adenylyl cyclase assay for anthrax
Thrombin Signal Transduction [2000]
. Protein-Protein Interaction of Protease Activated Receptor (PAR1) with
integrins, myosin, FAK, P130Cas, using rat aortic smooth muscle cells
(RASMC) and thrombin was studied using various parameters like [35S]-
Methionine lebelling, biotin labelled peptide, conjugation of PAR with
Sepharose, Immunoprecipitation.
. Transfection of RASMS with a growth factor receptor and cell
proliferation inhibition by various extracellular receptor binding
agents.
1993-1994: Visiting Researcher, University California Riverside, CA.
Yeast genetics in metal ion resistance : (Mehra et al 1996)
. Purification of phytochelatins and metallothioneins peptides from the
Yeast by size exclusion, ion exchange chromatography and reverse phase
HPLC.
. Determination of metals (Ag, Zn, Cu) in the peptides by Atomic
Adsorption Spectrometer.
. In vitro peptide and metal binding study using UV /Vis and
Luminescence Spectroscopy and reverse phase chromatography.
1990-1993: Postdoctoral Fellow, Bristol Myers Squibb, Princeton, NJ
Pre drug discovery in cancer research
. Hyperexpression of P21 ras oncoprotein in E-coli and its purification
(substrate for F.transferases).
. Purification of farnesyltransferase and molecular cloning of cDNAs
encoding a and b sub units.
. Molecular cloning of ptk-3, a gene encoding a new class of tyrosine
receptors. (PNAS, Sanchez et al 1994)
1989-1990: Postdoctoral Fellow, Medical University of South Carolina,
Charleston. SC
Palmitoyl-CoA synthetase purification
. Purification of palmitoyl-CoA synthetase from microsomal Sub cellular
fractions.
. Testing the hybridoma for the monoclonal antibodies production against
microsomal palmitoyl-CoA synthetase.
1985-1989 Research Officer, PGIMER, Chandigarh, India
Renovascular Hypertension
. Evaluation of anti-hypertensive effects of captopril, indomethacin
(oral) and prostacyclin (I/V)
. Developing RIA (iodination of angiotensin 1) for PRA in clinical and
Experimental Hypertension
1984-1985 Research Officer, Central Research Institute, Kasauli, India.
Rabies diagnostic, Influenza vaccine
. Cultivation of Rabies virus in suckling mice brain and cell line..
. Conjugation of HR Peroxidase to ant-rabies antibodies to develop
direct immunoperoxidase technique as diagnostic tool for rabies.
. Cultivation of Influenza viruses, from clinical samples, in egg yolk
and their serotyping by hemagglutination inhibition assay, using
patients antisera from the preceding years.
1978-1984: Ph.D, Program Dept of Microbiol, Panjab
University.Chandigarh India
Diabetes Immunology
. Purification of human pancreatic antigens, immunoprecipitating sera
from the patients with type-1 diabetes mellitus
. localization of these antigens on human pancreatic section by indirect
immunoperoxidase technique using antiserum raised in rabbits against
the purified antigens.
TECHNICAL SKILLS
A. Protein Chemistry
1. Recombinant Protein Purification
Hyper-expression and purification of recombinant proteins in E.Coli
[laboratory Scale (6-10 L)]: 1.
GST fusion Protein: Ras & PLAP protein
His tagged fusion protein: Acetyl choline receptors subunits, (alfa,
gamma, epsilone), CdFabK.,
Dihydrofolate reducatase (DHFR) fusion protein: Mouse translation
initiation factor (elF-4E)
2. Cellular Proteins Purification
Purification to homogeneity of the followings by NH4SO4, precipitation,
Gel filtration, Ion exchange, Hydrophobic interaction, affinity
chromatography, FPLC, HPLC and reverse phase HPLC and elecro-elution:-
Proteins : Human pancreatic antigen
Enzymes: Palmitoyal CoA ligase, Farnesyltransferase, Phospholipase A2,
CdFabK
Receptors: Acetyl choline receptors (AChR) from Torpedo ray fish, TE671
cell line
Antibodies: Monoclonal (M35, Ciq) and Polyclonal (rabbit PLAP, rat IL-
6,)
Peptides: Phytochelatins in Yeast, AChR binding Snake venom peptide
3. Characterization of Protein:-
Characterization the purified proteins by SDS-PAGE (Commassie B & Silver
staining), IEF, Western blot, RIA, ELISA, FACS, Immunoprecipitation,
Immunoperoxidase & Immunofluorescent, UV/visual
Fluoro/Spectrophotometery, Maldi-Mass Spectrometery. Enzyme activity,
autoradiography
4. Protein Conjugation:-
Experienced in conjugation of protein, antibodies, and peptides with
Protein A- Sepharose, Alexa 488, 125-Iodine, Horse raddish peroxidase.
Preparation of immunogen by conjugating peptides with Keyhole Limpet
Hemacyanin (KLH) M.Wt. ( 106 by covalently linking by EDC (1-ethyl-3-(3-
dimethylaminopropyl) carbodiimide hydrochloride). Metabolic lebelling of
cellular proteins with [35S] Methionine and phospholipids with [3H]
Arachidoninc acid.
5. Protein concentration equilibration:
Using centrifugation/Ultra pressure flow cell filtration devices, PEG
precipitation, and protein quantitation, by Lowry, Bradford, UV methods.
B. Immuno cyto/histo/bio chemistry/Immunobiology
1. Conjugation of immunogen, immunization (mice rabbit), pAb and mAb
production and purification.
2. Detection of antigen and antibodies by immunodiffusion, immuno/
counter-immunoe-electrophoresis, Western blot, ELISA, RIA, FACS analysis,
Heamgglut. inhibition, Immuno-peroxidese/flourescence.
3. Genotyping (HLA- DQ6, DQ8, DR4) by PCR.
C. Molecular Biology
1. Extraction of plasmid and genomic DNA. Restriction enzyme digestion.
Agarose gel electrophoresis, Purification of DNA by CsCl2 density
gradient / kits, and electroelution
2. Preparation of competent cell, ligation, Bacterial transformation by
heat shock/electroporation, transfection of various cell lines,
Construction of genomic DNA library. Recombinant Proteins expression.,
In vitro transcription and translation.
3. Northern Blot, RT-PCR, in situ hybridization, nucleotide sequencing by
dideoxy and thermal cycling,
4. Skilled in the use of bioinformatics databases, programs and web-
tools. (e.g. NCBI GenBank/GenPept, EMBL/SWISS-PROT, Vector NTI, GCG,
DNA)
D. Cell Biology/ Virology/Microbiology/Parasitology/Histology
1. Cell lines cultivation (Raw, Caco2, HT29, BHK, J774, U937, RASMC,
TE671, Malignant Mesothelioma, Small and Non-small cancer cells,
hybridoma (M35, C3q),
2. IC50 of various drugs and anti sense oligonucleotides on mesothelioma
and lung cancer cells.
3. Protein-protein interaction study in signal transduction.
4. Cytolytic studies, Apoptotic studies, complement mediated
cytotoxicity, study, Histochemical studies
5. Sub cellular fractionation by ultracentrifugation and density
gradient. Separation of blood cell
6. Cultivation of influenza virus in egg for vaccine preparation
7. Cultivation of Rabies virus in mice brain and BHK cell line, LD 50 of
rabies virus in suckling mice.
8. Rabbit illeal loop, skin test and suckling mice gut assay for
enterotoxin activity, endotoxin testing.
9. Microbial culture and mutant preparation, complete identification of
bacteria.
10. Antibiogram of multiple Drug resistance in Salmonella and E.Coli.
E. Enzyme assay/ Biochemical quantitations
Protein farnesyltransferase and geranylgeranyltransferase.
Metabolic lebelling of phospholipids with [14C] Arachidoninc acid..
Phospholipase A2, C, D, and phospholipid analysis, Cyclo-oxygenases,
Adenylyl cyclase by thin layer chromatography (TLC).
Palmitoyl CoA synthetase, DNase, RNase, Alkaline and Acid phosphatase,
Amylase, Lipase.,
Plasma renin activity, by radioimmunoassay, amino acid analysis by paper
chromatography, IC50 and Enzyme kinetic of various enzymes (Line Weaver
Equation).
Glutathion, Blood glucose and cholesterol, Collagen in human heart
valve. Atomic Adsorption Spectrometery for metal detection
EDUCATION:
Ph.D. 1984 Microbiology &Immunology Panjab University,
Chandigarh, India
PUBLICATONS
1. Saini, S.S., and Klein, M.A Targeting cyclin D1 in non small lung
cancer and mesothelioma cells by antisense oligonucleotides. Anti
Cancer Res. 31: 3683-3690, 2011.
2. Wu, X., Tuzun, E., Li, J., Xiao, T., Saini, S.S., Qi, H., Allman, W.,
Christadoss, P. Ocular and generalized myasthenia gravis induced by
human acetylcholine receptor ? subunit immunization. Muscle Nerve.
45:209-216, 2012
3. Allman, W., Qi, H., Saini, S.S., Li, J Tuzun, E., Christadoss,
P. CD4 costimulation is not required in a novel LPS-enhanced model
of myasthenia gravis. J. Neuroimmunol 249: 1-7, 2012
4. Allman, W., Saini, S.S., T z n, E. Christadoss, P. Characterization
of Peripheral Blood Acetylcholine Receptor-Binding B cells in
Experimental Myasthenia Gravis. Cell Immunol. 271: 292-298, 2011.
5. Qi, H., Li, J, Allman, W., Saini, S.S., T z n, E., Wu, X.,
Curran, E.M., Estes, M., and Christadoss, P. Genetic deficiency of
estrogen receptor alpha fails to influence experimental autoimmune
myasthenia gravis pathogenesis. J. Neuroimmunol, 234: 165-167, 2011
6. Li, J., Qi, H., T z n, E., Allman, W., Y?lmaz. V., Saini, S.S.,
Deymeer, F., Saruhan-Direskeneli, G., Christadoss, P. Mannose-binding
lectin pathway is not involved in myasthenia gravis pathogenesis.
J Neuroimmunol. 208: 40-45, 2009
7. T z n E, Li J, Saini S.S, Yang H, Christadoss P. Targeting classical
complement pathway to treat complement mediated autoimmune diseases.
Adv Exp Med Biol 632:265-72, 2008
8. Christadoss, P., T z n, E., Li, J., Saini, S.S., Yang. H, .Classical
complement pathway in experimental autoimmune myasthenia gravis
pathogenesis. Ann N Y Acad Sci. 1132:210-***-****
Qi, H., T z n, E., Allman, W., Saini, S.S., Penabad, Z.R.,
Pierangeli, S., Christadoss, P. C5a is not involved in experimental
autoimmune myasthenia gravis pathogenesis. J. Neuroimmunol.. 196, 101-
106: 2008
9. Li, J., Tuzun, E., Wu, X., Qi, H., Allman, W., Saini, S.S.,
Christadoss, P. Inhibitory IgG receptor FcgRIIB promotes experimental
autoimmune myasthenia gravis pathogenesis by stimulating IL-6 and IL-
10 production. J. Neuroimmunol 194, 44-53 2008
10. Yang, H., Wu., B., T z n, E., Saini, S.S., Li., Jing., Allman, W.,
Higgs, S., Xiao T.L., Christadoss P. A New Mouse Model of
Autoimmune Ocular Myasthenia Gravis. Invest Ophthalmol Vis Sci, 48,
5101-5111: 2007
11. T z n, E., Li, J., Saini, S.S., Yang H, Christadoss, Pros and cons
of treating Murine Myasthenia Gravis with anti-C1q antibody . J
Neuroimmunol. 182 167-76. 2007
12. Liu R, La Cava, A., Bai, X.F., Jee, Y., Price, M., Saini, S.S.,
Campagnolo, D.I., Christadoss, P., Vollmer, T.L., Van Kaer, L., Shi,
F.D. .Cooperation of invariant NKT cells and CD4+CD25+ T regulatory
cells in the prevention of autoimmune myasthenia. Liu, R., J Immunol.
2005 175 :7898-904. Appeared as- Correction for Liu et al., J .
Immunology 2006, 176:7130
13. T z n E, Saini S.S, Morgan,B. P., Christadoss.P. Complement regulator
CD59 deficiency fails to augment susceptibility to actively induced
Experimental Autoimmune Myasthenia Gravis. J Neuroimmunol. 181, 29-
33: 2006.
14. T z n E, Saini S.S, Yang H. Alagappan D, Higgs S. Christadoss P.
Genetic evidence for the involvement of Fc gamma receptor III in
experimental autoimmune myasthenia gravis pathogenesis J.
Neuroimmunology.174, 157-167: 2006
15. T z n, E., Saini, S.S., Ghosh, S., Rowin, J., Meriggioli, M.N.
Christadoss, P. Predictive value of serum anti-C1q antibody levels in
myasthenia gravis. Neuromuscular Disorders16, 137-143;2006
16. Saini, S.S, T z n, E., Christadoss. P. The cDNA of mouse skeletal
muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor
alpha subunit J. Neuroimmunology. 169 177-179: 2005
17. Saini, S.S., Gessell-Lee, D., Peterson, J.W. Cox-2-specific inhibitor
celecoxib inhibits adenylyl cyclase. Inflammation 27, 79-88: 2003
18. Saini, S.S, Chopra, A.K. and Peterson, J.W. Melittin-mediated
release of [3H]-Oleic acid from E. coli cells is dependent upon heat
and trypsin-sensitive factor(s) in human serum. Toxicon 38, 1077-
1086: 2000
19. Saini, S.S, Chopra, A.K. and Peterson, J.W. Melittin activates
endogenous Phospholipase D during cytolysis of human leukemia cells.
Toxicon 37, 1605-1619: 1999
20. Peterson, J.W., Boldogh I, Popov, V.L., Saini, S.S., and Chopra A.K.
Anti-inflammatory and antisecretory potential of histidine in
Salmonella-challenged mouse small intestine. Lab Invest 78, 523-534:
1998
21. Saini, S.S., Peterson, J.W. and Chopra A.K. Melittin binds to
secretary phospholipase A2 and inhibits its enzymatic activity.
Biochim. Biophys Res. Commun. 238, 436-442; 1997
22. Peterson, J., Klimpel, G., Chopra. A., Saini, S., Dickey, W., and
Gourley, W. Phospholipase A2 activating protein in inflammatory
bowel disease. Gut. 41(6):859-860: 1997
23. Peterson, J.W., Dickey, W.D., Saini, S.S., Gourley, W.K., Klimple
G.R. & Chopra A.K. A role for phospholipaseA2 activating protein in
idiopathic inflammatory bowel disease. Gut 39, 698-704: 1996
24. Peterson, J.W., Saini, S.S., Dickey, W.D., Klimple, G.R., Bomalasky,
J.S., Clark, M.A., Xu X-Jing & Chopra A.K. Cholera toxin induces
synthesis of Phospholipase A2-Activating protein. Infection &
Immunity. 64, 2137-2143: 1996
25. Mehra, R.K., Tran, K., Scott, G.W., Mulchandani, P., & Saini S.S.
Ag(1)-binding to phytochelatins. J. Inorganic Biochem. 61, 125-142:
1996
26. Sanchez, M.P., Tapley, P., Saini, S.S., He, B, Pulido, D. & Barbacid,
M. Multiple protein kinases in rat hippocampal neurones : Isolation
of Ptk3 a receptor expressed in prolifer-ative zones of the
developing brain. Procd. Natl Acad. Sci. (USA). 91, 1819-1823: 1994
27. Saini, S.S., Rai, R. and Chakravarti, R.N. (1989) Anti-hypertensive
effects of indomethacin in two kidney one renal wrap model in rhesus
monkeys. Med. Sci. Res. 17, 371-372: 1989
28. Tiwari, R.P., Virdi, J.S., Saini, S.S., Gupta, L.K. & Vadehra, D.V.
Development of chicks exposed to aflatoxin B1 during embryogenesis.
Indian J. Animal Sci. 59, 1473-1474: 1989
29. Virdi, J.S., Tiwari, R.P., Sexena, M., Khanna, V., Singh, G., Saini,
S.S. & Vadehra, D.V. (1989). Effect of aflatoxin B1 on the immune
system of the chick. J. Appl. toxicol. 9, 271-275: 1989
30. Saxena, S.N., Kumari, N., Saini, S.S., Soni, D.V., Pahwa, R.K.,
Jaysheela, M. Surveillance of Salmonella in India for drug
resistance. Indian J.Med. Sci. 43, 145-150: 1989
31. Saini, S.S., Tiwari, R.P., Bhalla, T.C., Gupta, L.K. and Vadehra,
D.V. A method for isolation of pancreatic antigens reacting with
sera from Type 1 diabetes. Indian J. Com. Microbiol. Immunol.
Infect. Dis. 9, 182-186: 1988
32. Saini, S.S., Tiwari, R.P., Bhalla, T.C., Singh, G. and Vadehra D.V.
Incidence of pancreatic auto-antibodies in relation to fasting blood
glucose level in type 1 diabetes. Indian J. Com. Microbiol. Immunol.
Infect. Dis. 9, 144-147: 1988
33. Singh, J., Tiwari, R.P., Singh, S.(Saini. S.S.), Singh, G. and
Vadehra, D.V. Biochemical and Immunogical effect of aflatoxin in
rabbit. Toxicology letters 35, 225-230: 1987
34. Tiwari, R.P, Mittal, V., Saini, S.S., Bhalla, T.C., Singh, G. and
Vadehra, D.V. Effect of metal ions in aflatoxin production by
Aspergillus parasiticus. Folia Microbiol. 31, 124-127: 1986
35. Tiwari, R.P., Mittal, V., Saini, S.S., Bhalla, T.C. Singh, G. and
Vadehra, D.V. Effects of fatty acids on aflatoxin productin by
Aspergillus parasiticus. Folia Microbiol. 31, 111-123: 1986
36. Bhalla,T.C., Dani, H.M., Vadehra, D.V. Tiwari, R.P. and Saini, S.S..
Evaluation of five different isolation procedures for preparation of
microsomes from hypocotyl of Cicer arietinum seedling. Cytobios . 46,
7-15: 1986
37. Tiwari, R.P., Singh, G., Bhalla, T.C., Saini, S.S., Gupta, L.K. and
Vadehra D.V. Mechanism of action of aflatoxin B1. J. Bioscience 10,
145-151: 1985
38. Tiwari, R.P., Singh, G., Saini, S.S., Gupta, L.K. and Vadehra, D.V.
Cell membrane as the target of aflatoxin B1 action. IRCS-Medical
Science. 13, 728-729: 1985
39. Tiwari, R.P. Dham, C.K., Saini, S.S., Bhalla, T.C., and Vadehra, D.V.
Mechanism of action of aflatoxin B1 in B. megatherium. Appl.
environ. Microbiol. 49, 904-907: 1985
40. Tiwari, R.P., Dham, C.K., Saini, S.S., Bhalla, T.C., Gupta, L.K. and
Vadehra, D.V. Mechanism of inhibtory effects of aflatoxin B1 in E.
Coli and S. typhi. J. Gen. Appl. Microbiol. 30: 419-426: 1984
Acknowledgement:
1. Sequencing of Grb2 in "Molecular cloning of the mouse grb2 gene:
differential interaction of the Grb2 adaptor protein with epidermal
growth factor and nerve growth factor receptors. K L Suen, X R
Bustelo, T Pawson, and M Barbacid Mol Cell Biol, 1993, 13, 5500-
5512."
2. Sequencing of Farnesyltransferase and substrate modification for
FTAse and GGTase in "Purified yeast protein farnesyltransferase is
structurally and functionally similar to its mammalian counterpart.
Gomez R, Goodman LE, Tripathy SK, O'Rourke E, Manne V, Tamanoi F.
Biochem J.
ABSTRACTS (Paper/poster presented in Conferences/symposium)
1. Allman, WR., Saini, S.S., Christadoss, P Frequency of acetylcholine
receptor specific B cells correlates with experimental myasthenia
gravis severity. Journal of Immunology, 2009, 182, 137.5
2. Allman, WR., Qi. H., Saini, S.S., Christadoss, P., CD4+ T cell
independent LPS induced experimental autoimmune myasthenia gravis
(EAMG). FASEB Journal 22:668.7 2008
3. Allman, W., Qi, H., Saini, S.S., Li, J., Christadoss,P. TLR4 enhances
the development of LPS-Acetylcholine receptor induced autoimmune
myasthenia Gravis. 9th International Congress of NeuroImmunology,
Fort Worth, Texas, USA, October 26-30, 2008
4. T z n E, Oto A, Saini SS, Christadoss P. The influence of different
MHC Class II alleles on the immuneresponse to acetylcholine receptor
alpha3 subunit. 43rd National Neurology Congress,Antalya, Turkey. 2007
Abstr # p:100-101.
5. Christadoss.P, T z n E, Saini SS, Yang H Classical complement pathway
in experimental autoimmune MG pathogenesis N.Y.A.S and M.G Foundation
of America, The 11th International Conference on Myasthenia Gravis and
related disorders, Chicago, May 13-16, 2007 Abstract 2005; p:5.
6. Li, J., T z n E., Qi, H.B., Allman, W., Saini SS, Christadoss P.
Inhibitory IgG receptor FC?RIIB potentiates murine MG pathogenesis by
augmenting Il-6 and Il-10 production N.Y.A.S and M.G Foundation of
America, The 11th International Conference on Myasthenia Gravis and
related disorders at Chicago May 13-16, 2007 Abstr# 28.
7. Li, J., T z n E., Qi, H.B., Allman, W., Saini SS, Bednov, A.,
Christadoss.P. Fc ?RIIB and Experimental Autoimmune Myasthenia
Gravis. Annual Meeting of Clinical Immunology Society, School in
Systemic Autoimmune Diseases, SantaFe, New Mexico March 14-18, 2007.
8. T z n E, Li, J., Saini SS, Christadoss. P Immune complexes affect
EAMG severity via FCgamma receptor III mediate Complement production
N.Y.A.S and M.G Foundation of America, The 11th International
Conference on Myasthenia Gravis and related disorders Chicaco, 2007.
Abstr# 9
9. Allman, W., Li, J., Qi, H.B., Saini, SS, Christadoss.P. Activation
and survival of autoreactive B cell populations in experimental
Mysthenia Gravis (EAMG) McLaughlin Annual Colloquium on Infection &
Immunity (2006, Galveston, Texas, USA) 2006, Abstr#1.
10. Li, J., T z n E., Qi, H.B., Allman, W., Saini SS, Christadoss.P.
Inhibitory IgG receptor Fc ?RIIB ptentiate murine Myasthenia Gravis
pathogenesis by augmenting IL-6 and IL-6 production. McLaughlin
Annual Colloquium on Infection & Immunity Galveston, Texas, USA 2007;
Abstr# 40
11. Li, J., T z n E, Saini SS, Bednov, A., Morgan,BP., Christadoss.P Anti-
DAF Antibody Treatment Augments the Incidence and Severity of ocular
MG in HLA-DQ8 Mice Immunized with H-AChR ? Subunit. Scientific Session
Myasthenia Gravis Foundation of America at Chicago 2006, Abst r#
12. T z n E, Saini S, Yang H, Higgs S, Christadoss P. Prevention of
experimental autoimmune myasthenia gravis by anti-C1q antibody is
associated with reduced anti-AChR IgG2b, IL-6 and IFN-alpha production
(Poster presentation, English). Myologie (2005, Nantes, France)
Abstract, p:252.
13. T z n E, Yang H, Wu B, Saini S, Bednov A, Ghosh S, Xiao T, Christadoss
P. Ocular myasthenia gravis in HLA transgenic mice immunized with
acetylcholine receptor alpha subunit expressed in E. coli . Myasthenia
Gravis Foundation of America Scientific Session (2005, San Diego,
California, USA)
14. T z n E, Saini SS, Yang H, Higgs S, Christadoss P. Anti-C1q antibody
prevents experimental autoimmune myasthenia gravis by reducing anti-
AChR IgG2b, IL-6 and IFN-? . First Annual National Meeting of the
Association of Scientists of Indian Origin in America (2005, San
Antonio, Texas, USA) Abstract Booklet, 2005; p:19.
15. Christadoss P, T z n E, Saini SS, Yang H. Anti-C1q treatment prevents
murine myasthenia gravis by reducing IL-6 and IFN-? production and
neuromuscular junction complement deposition (Poster presentation,
English). XXth International Complement Workshop (2004, Honolulu,
Hawaii, USA) Abstract Booklet (Molecular Immunology, supplement to
volume 41(2-3),2004); p:221.
16. Saini S.S, T z n E, Ghosh S, Yang H, Christadoss P. Anti-C1q antibody
prevents and ameliorates experimental autoimmune myasthenia gravis
(Poster presentation, English). IBC's 2nd Annual International
Conference (2004, San Diego, California, USA) Abstract Booklet, 2004;
p:3063.
17. T z n E, Saini S.S, Ghosh S, Christadoss P. Anti-C1q antibody in
prevention and treatment of antibody mediated autoimmune diseases.
International Education Week Poster Competition (2004, Galveston,
Texas, USA) Abstract Booklet, 2004; p:8.
18. Peterson, J.W., Gessell-Lee, D., Saini, S.S., Weisbaum, D., Lemoins,
P., Morris, A.P., Fernandez, W.A., Schein, C.H. and Braun, W. Novel
inhibitors of cholera toxin-induced intestinal fluid secretion. 10th
Asian Conference on Diarrhoeal Diseases and Nutrition (ASCODD), Dhaka,
Bangladesh 2003
19. Peterson, J.W., Dickey, W.D., Saini, S.S., Gourley, W.K., Klimple G.R.
& Chopra A.K. .A role for phospholipase A2 activating protein in
idiopathic inflammatory bowel disease. Annual meeting of American
Gastroenterology Association at San Francisco 1996, May 19-22.
20. Peterson, J.W., Saini, S.S., Gessell-Lee, D.L. (2002). PGE2-l-
histidine is a potent inhibitor of adenylyl cyclase that reduces the
secretory response of cholera. Annual meeting of American
Gastroenterology Association at San Francisco May 2002
21. Manne, V., Saini, S.S. and Barbacid, M. Multiplicity of
farnesyltransferase. NATO Advance Workshop on,"The Super Family of Ras
Related Genes". Crete, Greece May 17-21; 1991
22. Saini, S.S., Tiwari, R.P., Singh, G. and Vadehra, D.V. A method for
isolation of pancreatic antigen (s) reacting with Type-1diabetic sera.
11 International Symposium on Electron Microscope and Biophysics.
Punjab University Chandigarh, 1986 January 31-February 3, Abstr. 42-B
Contact this candidate