Manager Training

Mr. Daniel Mikos

?**** S. Latrobe Chicago, IL, **638 ? Phone: 1-708-***-**** ? E-

mail: ************@*****.***

SUMMARY

I am a Biologist looking for opportunities. I have 8 years of hands-on lab

experience in molecular genetics, cell and tissue biology, protein array

analysis, nanofabrication, production, research and development,

microscopy, and quality inspection offering a broad background and

knowledge of different laboratory equipment methods and techniques

including working in BSL-1, -2, &-3 facilities.

PROFESSIONAL EXPERIENCE

. NanoInk Inc: Lead Research Associate: Reported to Dr.

Harris Jamil, Roz Helfrich, and Frank Lantz ~October

1st 2010-Feb 15th 2013

Responsibilities: I served as a liaison between the R&D dept.

and the Production dept. overseeing the day to day management

and upkeep of the lab, chemicals and bio-printing machines. I

ran several different types of highly specialized personalized

ELISA's using customer samples and reporting findings at weekly

meetings (using Microsoft Excel, Word and Power Point). I was

also responsible for pipetting(multi and single-channel, air and

positive displacement, volumetric, robot assisted, and micro

pipetting), GLP compliancy, centrifugation(micro and high-

speed), fluorescent microscopy, atomic force microscopy, assay

development using the patented Dip Pen Nanolithography

Technology, contact angle measurements, spectroscopy, antibody

selection and optimization, document writing, plasma/ozone

cleaning of microarray chips, SEM, optimizing the use and design

of the equipment for each assay, and I was responsible for many

other types of equipment, experiments, and protocols.

. Loyola University Chicago: Cardinal Bernardin Cancer

Center, Hematological malignancy program, Laboratory

Technician 1: Reported to Dr. Nancy Zeleznik-Le

~November 2008-June 2010

Responsibilities: We had studied the binding affinity of Mixed

Linkage Leukemia (MLL) binding partners HDAC1 and HPC2 using

PCR, pipetting (multi and single-channel), centrifugation(micro

and high-speed), restriction digests, cloning, gel

electrophoresis, gateway recombination cloning, T3 and SP6

transcription reactions, phenol extraction/precipitation

reactions, spectroscopy, western blot (radioactive and non-

radioactive), GST pull-down assay, phosporimaging, gene

modification software, radiation safety training, hazardous

materials handling training, tissue & cell cultures, viral

transfection, DNA purification from human bone marrow, general

laboratory management & maintenance, fluorescent microscopy.

. Loyola University Chicago, Biology Department Lab

Coordinators Assistant: student worker ~August 2007-May

2008

. Loyola University Chicago, enrollment operations:

student worker ~August 2005-May 2008

Educational and Academic Background

Moraine Valley Community College: Associates in Science~ Graduated Cum

Laude

Loyola University Chicago: Bachelors in Science, Major in Biology

Presentations

"Evaluation of the Sindbis Virus as a Viral Vector", Presented at the

Loyola University Undergraduate Research Presentations, 9-Dec-2007

"Neuroscience in the Classroom: Evaluation of VSM-1", Presented for the

Society For Neuroscience(SFN) at DePaul University, 28-Mar-2008

TECHNICIAL SKILLS

Experienced in the principles and practices of molecular biology such as:

~Sandwich enzyme-linked immunosorbent assay ~PCR ~Reagent preparation

~Restriction digests ~Ligations ~Cloning ~Nanotechnology ~Microfluidics

~Assay Development ~R & D to Production transitioning ~Document Writing

~Gel electrophoresis ~Gateway recombination cloning ~T3 and SP6

transcription reactions ~Phenol extraction/precipitation reactions

~Spectroscopy ~Thin films ~Western Blot (radioactive and non-radioactive)

~GST pull-down assay ~Phosporimaging ~Gene modification software

~Zebrafish embryo microinjection ~Mouse dissection ~Animal care,

maintenance and husbandry ~Sectioning using a microtome and sample

preparation ~Contact angles ~Transmission electron microscope proficiency

~Scanning electron microscope proficiency ~Atomic force microscopy

proficiency ~Confocal microscopy ~Radiation safety training ~Hazardous

materials handling training ~Tissue & cell cultures ~Viral transfection

~DNA purification from human bone marrow ~General laboratory management &

maintenance ~Plasma and Ozone Cleaning ~Dip Pen Nanolithography

Technology ~Fluorescent microscopy ~MEMS

Other Facts

I took Latin in college. I enjoy playing chess. I have held positions as

publicist, treasurer, inter-club council representative, vice-president

and president of various chess clubs. I received the Gold Medal of

Achievement from the Royal Rangers young men's group.

Excellent recommendations available upon request from

Dr. Harris Jamil NanoInk inc.: Laboratory Director

Frank Lantz NanoInk inc.: Director of Manufacturing

Roz Helfrich: NanoInk INC.: Laboratory Manager

Contact this candidate