Assistant Management

Amanjot Riar

*** **** ******, *** ****, Malden MA-02148 Mobile: 917-***-**** Email: *********@*****.***

PROFILE: A diverse research background that includes excellent research skills and proficiencies in biomedical sciences with

translational knowledge to apply basic scientific research into new approaches for the treatment of diseases.

SUMMARY

Extensive experience in vitro and in vivo neurobiology, pharmacology and toxicology, in vitro protein biochemistry, cellular

and molecular biology

Good understanding of AAALAC International OLAW/NIH and USDA for the conduct of in vivo studies

Good understanding of GLP and cGMP regulations. Maintaining SOPs along with excellent lab data management,

interpreting data and adhering to strict guidelines on documentation when recording data and reporting scientific results

Take initiative and can multi-task effectively to meet deadlines and work under high-pressure environment efficiently

Ability to work independently or lead projects effectively

Outstanding interpersonal, organizational, written and verbal communication skills

EDUCATION

Doctor of Philosophy 2009- Expected Feb 2014

Concentration: Pharmacology and Neuroscience

Graduate School of Biomedical Sciences, Texas Tech University Health Sciences Center (TTUHSC)

Master of Business Administration (MBA) 2012 - 2013

Concentration: General Business

Rawls College of Business, Texas Tech University (TTU)

Graduate Certificate Program 2012 - 2013

Academic Minor/Concentration: Leadership

Rawls College of Business, Texas Tech University (TTU)

Bachelor of Pharmacy (BS) 2003 - 2007

Sant Gadge Baba Amravati University, Maharashtra, India

Licensed Registered Pharmacist

Maharashtra State Pharmacy Council under the Pharmacy Act, India

RESEARCH EXPERIENCE

Research Assistant 2009 - 2014

Department of Pharmacology & Neuroscience, TTUHSC, Lubbock, TX

Role/Responsibilities: Setting up experimental design, executing research, co-coordinating research findings with teams,

troubleshooting, primary drafting of manuscript

1. Studied the teratogenic effects of maternal alcohol consumption on developing fetal brain in vitro

Developed in vitro alcohol models simulating human acute and chronic alcohol consumption

Characterized mammalian cerebral cortical neuroblast cell line using standard cell culture techniques

Examined the effect of prenatal alcohol exposure on proliferation of neuroblast using cell viability assays including

BrdU incorporation and trypan blue exclusion using vi-cell viability analyzer

Explored the toxic effect of alcohol on cell cycle progression using cell cycle analysis by flow cytometry

Performed cell differentiation studies using western blot analysis of various primitive and mature neuronal markers

Explored the role of tumor suppressor, Programmed cell death 4 (PDCD4) in alcohol-induced abnormalities of fetal

brain using western blotting, RT-PCR, siRNA and adenovirus methods

Examined the time and dose dependent effects of alcohol on PDCD4 protein and mRNA expression

Performed pulse-chase protein and mRNA stability experiments using chemical inhibitors such as cycloheximide and

actinomycin D

Cloning and deletion sequence analysis of promoter of rat PDCD4 gene

Studied the transcriptional regulation of PDCD4 using dual luciferase reporter assays

Discovered the role of Glycogen Synthase Kinase -3 β (GSK-3β) signaling transduction pathway in the regulation of

PDCD4 in brain neuroblast using Lithium chloride (FDA approved drug for the treatment of epilepsy and bipolar

disorders) & SB 216763 and siRNA against GSK-3β

Performed Protein/DNA array for evaluation of transcription factor regulated in response to alcohol

2. Acute toxicologic studies of paraquat (herbicide) on developing fetal brain in vivo

Routinely handled rodents (adults and embryonic & postnatal pups)

Assisted lab scientists in developing the in vivo model and evaluated the toxicity of paraquat in fetal brain

Assisted in intragastric gavage and intraperitoneal injections of paraquat to postnatal pups

Assisted in the assessment of lethality (LD50) of paraquat in postnatal pups

Assisted in determining the temporal distribution of paraquat in postnatal brain using HPLC based detection methods

Student Assistant May 2009 - July 2009

Cell Physiology & Molecular Biophysics, TTUHSC, Lubbock, TX

Handling and microinjection of RNA into Xenopus laevis oocytes

Performed electrophysiological recording in oocytes using two-voltage electrode clamp

Studied the effect of drug Capsaicin (used in dermatologic and peripheral disorders) on Na/K pump

OTHER EMPLOYMENT EXPERIENCE

Student Assistant Mar 2009 - May 2009

Hospitality Services, TTU, Lubbock, TX

Routinely handled 50 customers in a busy dormitory dining hall

Skillsets developed- extensive development of customer relations and interface communications, rapidly incorporated

information, anticipate customer concerns, cultivated organizational culture, maintained safe and hygienic work

environment, high level skills in dispute resolution and financial management

RESEARCH SKILLS AND TECHNIQUES

Molecular Biology: DNA/RNA isolation and purification from cells, molecular cloning, sequence analysis, agar plate and

broth preparation, bacterial inoculation, bacterial transformation, agarose gel electrophoresis, PCR analysis, Real Time-PCR

analysis, immunoprecipitation (IP) assays, Protein/DNA Array

Cell Biology/Biochemistry: Primary cortical neuronal cultures, Primary cortical astrocyte cultures, mammalian cell culture

and maintenance including rat cortical neuroblast, rat pheochromocytoma (PC12), human neuroblastoma (SH-SY5Y) and

human embryonic kidney (HEK) 293 cell line, protein determination using bradford, SDS-PAGE, western blot analysis, cell

transfection and transduction, luciferase assay, electrophoretic gel mobility shift assay (EMSA), cell based assays, cell

viability assays, cytotoxicity assays, luminescence assays, fluorescence assays, cell cycle analysis, flow cytometry (FACS)

analysis, immunocytochemistry (IC), immunohistochemistry (IHC), Immunofluorescence (IF), ELISA assays,

Spectrophotometry, virus purification

Electrophysiological & Biophysics techniques: Two-Electrode Voltage Clamp

Analytical tools: Assisted in HPLC studies, UV/Vis Spectroscopy

Animal in vivo models: Rodent handling, intra-gastric gavages (IG), intraperitoneal injections, tissue harvesting, brain tissue

dissection,

Computer Skills: Microsoft word, Excel, Powerpoint, GraphPad InStat, Image J, Scion Image, Adobe Photoshop, Pub med

sequence analysis and literature searches, Blast, UCSC Genome Browser

PUBLICATIONS

1. Amanjot Kaur Riar, Madhusudhanan Narasimhan, Mary Latha Rathinam, Dhanashree Vedpathak, George I. Henderson

and Lenin Mahimainathan. Ethanol Mediates the Transcriptional Activation of Programmed Cell Death 4 (PDCD4) through

GSK-3β signaling pathway in Cortical Rat Brain Neuroblasts (To be submitted to journal of PLOS ONE)

2. Amanjot Kaur Riar, Madhusudhananan Narasimhan, Mary Latha Rathinam, George I. Henderson and Lenin

Mahimainathan. Ethanol Inhibits the Proliferation of Tbr2-positive Rat Cortical Basal Progenitors (To be submitted to

journal of Alcoholism: Clinical and Experimental Research)

3. Narasimhan M, Rathinam M, Riar A, Patel D, Mummidi S, Yang HS, Colburn NH, Henderson GI, Mahimainathan L.

Programmed cell death 4(PDCD4): a novel player in ethanol-mediated suppression of protein translation in primary cortical

neurons and developing cerebral cortex. Alcohol Clin Exp Res.2013 Jan; 37(1): 96-109

4. Rathinam ML, Watts LT, Narasimhan M, Riar AK, Mahimainathan L, Henderson GI. Astrocyte mediated protection of fetal

cerebral cortical neurons from rotenone and paraquat. Environ Toxicol Pharmacol.2012 Mar;33(2):353-60

5. Narasimhan M, Mahimainathan L, Rathinam ML, Riar AK, Henderson GI. Overepression of Nrf2 protects cerebral cortical

neurons from ethanol-induced apoptotic death. Mol.Pharmacol.2011 Dec; 80 (6): 988-99

CONFERENCE PRESENTATIONS (Selected from over 10)

1. A.K.Riar, M.Narsimhan, M.L.Rathinam, D.Vedpathak, G.I.Henderson, L.Mahimainathan (2013). Ethanol Transcriptionally

Activates Programmed Cell Death 4 (PDCD4) in Rat Cortical Brain Neuroblasts. Abstracts–Posters. Alcoholism: Clinical

and Experimental Research, 37: 11A–253A. Poster presentation delivered at the Research Society on Alcoholism,

Orlando, Florida

2. A.Riar, M.Narasimhan, M.L.Rathinam, D.Vedpathak, D.Patel, G.Henderson, L.Mahimainathan (2012). Role of

Programmed Cell Death 4 (PDCD4) in Ethanol-Induced Dysregulation of Neurogenesis. Abstracts Posters. Alcoholism:

Clinical and Experimental Research, 36: 11A–303A. Poster presentation delivered at the Research Society on

Alcoholism, San Francisco, California

3. A.Riar, M.Narasimhan, M.L. Rathinam, G.I. Henderson and L. Mahimainathan (2013). Ethanol Inhibits the Proliferation of

Cortical Basal Neuronal Progenitors. Poster presentation delivered at the Gender-Specific Medicine & Women’s Health

Symposium, Lubbock, Texas.

4. A.Riar, M.Narasimhan, M.L. Rathinam, G.I. Henderson and L. Mahimainathan (2012). Role of Programmed Cell Death 4

(PDCD4) In Ethanol-Induced Dysregulation of Neurogenesis. Poster presentation delivered at Texas Research Society

on Alcoholism (TRSA) combined with Texas American Society of Addiction Medicine (TSAM) and Region 8

American Association of Addiction Psychiatry (AAAP), Bryan/College Station, Texas

5. A.Riar, M.Narasimhan, M.L. Rathinam, G.I. Henderson and L. Mahimainathan (2011). Programmed Cell Death 4 (PDCD4)

As A Potential Mediator of Ethanol Induced Inhibition of Neuroblast Proliferation. Poster presentation delivered at Texas

Research Society on Alcoholism (TRSA), Lubbock, Texas.

TEACHING EXPERIENCE

Lab Mentor: Key role in training biotechnology interns for hands on experience in biomedical research with teaching them lab

techniques in areas including cell biology, molecular biology and biochemistry

SEMINARS PRESENTED

A. Graduate School of Biomedical Sciences, TTUHSC (Selected from over 9)

1. Journal Club Presentation, Title: Interaction between RAX and PKR Modulates the Effect of Ethanol on Protein Synthesis

and Survival of Neurons.

2. Research Presentation, Title: Programmed cell death 4 (PDCD4) as a potential mediator of ethanol-induced inhibition of

neuroblast proliferation.

3. Journal Club Presentation, Title: Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of

neural progenitor cells.

4. Research Presentation, Title: Role of Programmed Cell Death 4 (PDCD4) in ethanol-induced dysregulation of neurogenesis.

B. Rawls College of Business School, TTU

1. Management Consultant for Netflix Company, Course: Strategic and Global Management

2. Taught Chapter: Ethical Problems of Managers to a class of 36 MBA graduate students, Course: Leadership and Ethics

3. Taught Chapter: Communication in Internet Age to a class of 32 MBA graduate students, Course: Managing Organizational

Behavior and Organizational Design

4. IT Management Consultant for Case Studies, Course: Information Technology for Managers

LEADERSHIP SKILLS

1. Chair, Food Committee, Annual Student Research Week, TTUHSC, Lubbock 2013

2. Co-Representative for Rawls College of Business during Information Session, 2013

Graduate Student and Faculty Mentor Retreat, TTUHSC, Lubbock

3. Representative, Family Promise, Lubbock 2012

4. Representative, Annual Student Research Week, TTUHSC, Lubbock 2011

5. Representative, Bake Sale, TTUHSC, Lubbock 2011

AWARDS & CERTIFICATIONS

1. CH Foundation Scholarship, TTUHSC, Lubbock, Texas

2. Good Clinical Practice (GCP): Introduction to Clinical Trials and Drug Development, BioPharma Institute Program

3. Good Manufacturing Practice (GMP): Documentation and Record Keeping, BioPharma Institute Program

PROFESSIONAL MEMBERSHIPS/AFFILIATIONS

1. Research Society on Alcoholism

2. Texas Research Society on Alcoholism

3. Pharmacy Council of India

Names and addresses of 3 referees will be made available upon request

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