Customer Service Research

Curriculum Vitae

Florence Myrick

** ****'s Way Lane . Henderson, North Carolina 27537

Home: 252-***-**** . Email: *********@*******.***

Professional Profile

. Accomplished research professional with extensive experience in HIV and

Hepatitis B Virus research including phenotyping activities.

. Leverage extensive knowledge of molecular biology with research

techniques and comprehensive pharmaceutical understanding to develop

innovative methods that advance areas of research and development.

. Proven skills in delivering effective presentations to wide range of

audiences. Build strong, sustainable relationships with colleagues and

across all organizational levels.

. Core knowledge and skill areas include:

- HBV-RT Phenotyping; DNA Extraction; Method Development; Data

Analysis and Evaluation

- HIV Phenotyping; Cell Culture; GLP Standards; Novel Techniques;

Project Lifecycle Management

- PBMC Processing; Site-Directed Mutagenesis; Transfection;

Transformation; Staff Training & Mentoring

- Sequence Analysis; Database Design and Development; Laboratory and

Equipment Maintenance

- New/Emerging Technologies; Public Speaking & Communications; R&D

Initiatives; Thought Leadership

- On-Site Clinical Training; Drug Master Plate Development; Hepatitis

B and HIV Assays

Education

. Bachelor of Science in Biology, Elizabeth City State University,

Elizabeth City, North Carolina, May 1995

Academic Honors and Awards

. Guest Lecturer, Elizabeth City State University, School of Biology,

Elizabeth City, North Carolina

Research Interests

. Research interests are directed primarily toward sample research in a

variety of interrelated areas:

? Molecular Biology and Cell Culture

? Microbiology / Research Laboratory

? Biotechnology and Pharmaceutical Fields

Technical Skills

. Instruments: Roche Light Cycler 480, DiDeoxy Sequencing on ABI 377, COBAS

Amplicor Analyzer, Digene Hybrid Capture II, Affymetrix Genechip

Analyzer, Nano Drop Spectrophotometer, Invitrogen E-Gel System, Gene Amp

PCR System 9700, Microcentrifuge, Bio-Rad Gel Doc XR, Biotek Quant,

ELx50 Auto Strip Washer, Bio Rad Gene Pulser II, MJ PTC-100 Programmable

Thermal Controller.

. Methods/Techniques: Good Laboratory Practices (GLP), HBV RT Phenotyping

assay, Site Directed Mutagenesis, DNA Extraction, HIV Phenotyping assay

in MT2 cells & PBMC, Cell culture (Hep G2; PBMC, MT2, Magi,), Real Time

PCR, Electrophoresis, Ligation, Restriction Digest, DNA Purification,

Aseptic cell culture & reagent preparation techniques, HIV Genotyping by

Affymetrix and ABI, Extracting PBMCs from donor buffy coats, HBV Cloning

Assay, TOPO TA Cloning; ELISA

. Computer Skills: Microsoft Works, Word, Excel, Access, Power Point;

CalcuSyn, Table 2D Curve, Sequencher, Mac Synergy II, Sigma P lot/Sigma

Stat, KaleidaGraph

Highlights of Professional Experience

2nd Shift Laboratory Supervisor I, Infectious Disease Department, LabCorp -

Research Triangle Park, North Carolina - 01/2011 - present.

. Trains employees and monitors performance of all employees in work group.

. Schedules and prioritizes work by scheduling work hours to ensure

adequate coverage at all times especially during holidays.

. Coordinates the work of technologists as well as assigning and reviewing

work of technologists.

. Adheres to department as well as company-wide safety guidelines; follows

SOPs and GLP-Good Lab Practices

. Supervising high-through put daily laboratory duties.

. Follows and ensures the lab procedures for specimen handling and

processing, test analyses, reporting results and maintaining records of

patient test results.

. Troubleshoots the following: old specimen, incorrect specimen, QNS-

quantity not sufficient and all other problem coded

. Assisted in CLIA and CAP re-certification for department

. Reviews daily pending worksheets and collects turnaround time-TAT;

ensures that specimens are tested completely, accurately and within

established time frames.

. Daily contact with customer service to address client concerns.

. Perform assay paperwork which includes upload verification of released

results on Roche Amplipreps for HIV, HBV & HCV RT, HIV Qualitative tests

and initial review & verification of results for HCV Qualitative Test.

Senior Research Associate II, Clinical Virology Department, Gilead Sciences

- Durham, North Carolina - 06/1998-12/2010

. Created Site Directed Mutants - SDM for Hepatitis B Virus studies and

performed HBV RT phenotyping assay on the SDMs on a wide variety of

compounds.

. Maintained Hepatitis B Virus Research Laboratory and CO2 system. Analyzed

sequence of RT region as well as full Hepatitis B Virus genome.

. Completed extensive cell culture work with a variety of cell lines.

Functioned as a PBMC Collection Trainer at clinical sites in various

locations around the U.S.

. Created in-house method for processing PBMCs and traveled to clinical

trial sites in order to train personnel on procedure; additional trained

team members within and external to the department.

. Performed Hepatitis B Virus RT phenotyping assay, HIV RT assay, and HIV

Fitness assay. Developed drug master plates of various compounds and

concentrations.

. Maintained Hep G2 cell line, PBMC (Peripheral Blood Monocyte Cells), MAGI

and MT2 cell lines, HIV laboratory, and CO2 incubator system.

. Managed HIV Research Recombinant database and created HIV Recombinant

Viruses database for HIV studies and Site Directed Mutants for clinical

studies.

. Assisted in CLIA certification for department and Carolina Star

certification for the site.

. Department safety representative for site.

Technologist I, LabCorp - Research Triangle Park, North Carolina -

12/1996-06/1998

. Ran samples for HIV testing using Roche Amplicor Kit for throughput

testing.

. Ran HCV samples on quantitative assay.

. Assisted with various laboratory supply maintenance activities.

Scholarly Contributions

Sample Journal Publications

. Joy T. Feng, John K. Ly, Florence Myrick, Derrick Goodman, Kirsten L.

White, Evguenia S. Svarovskaia, Katyna Borroto-Esoda and Michael D.

Miller."The Triple Combination of Tenofovir, Emtricitabine and Efavirenz

Shows Synergistic Anti-HIV-1 Activity In Vitro: A Mechanism of Action

Study". Manuscript submitted to JBC, November 2008.

. Tomas Cihlar, Adrian S. Ray, Constantine B. Goojamra, Lijun Zhang, Hon

Hui, Genevieve Laflamme, Jennifer E. Vela, Deborah Grant, James Chen,

Florence Myrick, Kirsten L. White, Ying Gao, Kuei-Ying Lin, Janet L.

Douglas, Neil Parkin, Anne Carey, Rowchanak Pakdaman, and Richard

Mackman. "Novel nucleotide RT inhibitor and its prodrug GS-9131.

Antimicrobial Agents Chemotherapy August 2007.

. Evguenia S. Svarovskaia, Joy Y. Feng, Nicolas A. Margot, Florence Myrick,

Derrick Goodman, John K. Ly, Kirsten L. White, Nilima Kutty, Ruth Wang,

Katyna Borroto-Esoda, Michael D. Miller. "The A62V and S68G Mutations in

Human Immunodeficiency Virus Type 1 Reverse Transcriptase Partially

Restore the Replication Defect Associated with the K65R Mutation" Journal

of Virology, June 2007.

. Feng, J.Y., Myrick, F.T., Margot, N.A., Mulamba, G.B., Rimsky, L., Selmi,

B., Canard, B., and Borroto-Esoda, K. "Virologic and Enzymatic Studies

Revealing the Mechanism of K65R and Q151M-Associated HIV-1 Drug

Resistance towards Emtricitabine and Lamivudine." Nucleosides,

Nucleotides & Nucleic Acids. 25 (1), page 89-107, 2006.

. Feng, J.Y., Myrick, F.T., Selmi, B., Deval, J., Canard, B., and Borroto-

Esoda, K. (2005). "Effects of HIV Q151M-Associated Multi-Drug Resistance

Mutations on the Activities of ?-D-1',3'-Dioxolane Guanine." Antiviral

Research 66, 153-158, March 2005.

. Borroto-Esoda, K., Myrick, F., Feng, J., Jeffrey, J., and Furman, P. In

Vitro Combination of Amdoxovir (DAPD) and the Inosine Monophosphate

Dehydrogenase Inhibitors Mycophenolic Acid and Ribavirin demonstrates

potent activity against wild type and drug resistant variants of HIV-1.

Antimicrobial Agents and Chemotherapy, June 2004.

. Andrea Snow, Florence Myrick, Charles Wakeford and Bruce MC Creedy.

Evaluation of three commercially available HBV viral load assays:

Dynamic range, precision and clinical utility. Frontiers in Viral

Hepatitis. 165-172. 2003.

. Mewshaw, J., Myrick, F., Wakefield, D., Hooper, B., Harris, J., McCreedy,

B., and Borroto-Esoda, K. Dioxolane Guanosine, the Active Form of the

Prodrug Diaminopurine Dioxolane, is a Potent Inhibitor of Drug-Resistant

HIV-1 Isolates From Patients Failing Standard Nucleoside Therapy.

Journal of AIDS & Human Retrovirology, January 2002.

Presentations and Workshops

. Resistance Surveillance for up to 144 Weeks in HB3Ag+ and HBeAg-

Hepatitis B Patients Treated with Tenofovir DF Showed No relationship

Between Virologic Breakthrough and Emergence of Genotypic Changes in HBV

Polymerase, 07/2009

. In Vitro Tenofovir Sensitivity of HBV Populations from Clinical Specimens

Containing rt A181T/V and/or rtN236T, 07/2009

. The HIV-1 RT Mutant Q151L Shows Decreased Replication Capacity, Selective

High-Level Resistance to GS, 07/2009

. Pre-Existing Low-Levels of the K103N HIV-RT Mutation is a Risk Factory

for Treatment-Naive Patients Failing a 3TC+ZDV+EFV Regimen, 07/2008

. The Triple Combination of Tenofovir, Emtricitabine and Efavirenz Shows

Synergistic Anti-HIV-1 Activity In Vitro, 07/2007

Professional Affiliations

. Member, International Society of Antiviral Research (ISAR)

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