OBJECTIVE:
To capitalize upon my extensive laboratory experience, education. and
personal attributes to further my career in biotechnology or the
pharmaceutical industry.
EDUCATION:
Rutgers University, New Brunswick, New Jersey - Bachelor's Degree; BS in
Biological Sciences - 2009
PROFESSIONAL EXPERIENCE:
6/12 to 8/13 - Senior Lab Scientist - New York Genome Center
New York, NY
. Installed and organized a high throughput pilot lab
. Validated, operated, and maintained Illumina Sequencing/Array
platforms
. Managed the sequencing of pilot projects
. Maintained an internal database
. Operated, maintained, and refined high throughput robotics
. Provided feedback and drafted compliance documentation for GxP (Good
Manufacturing Practice, Good Documentation Practice, Good Clinical
Practice, Good Lab Practice)
. Authored, enacted, and oversaw the implementation of Lab SOP's
(standard operation procedures)
. Verified clarity, completeness, and accuracy of technical
documentation
. Troubleshot with bioinformatitions on pipeline data delivery
. Trained junior techs on sequencing platforms and assays
. Facilitated the scaling up of the pilot lab to fully functioning
Genomics Lab
. Evaluated new technologies and served as point of contact for
specialized lab instrument vendors
6/2009 to 6/2012 - Senior Lab Technician - Rutgers University
New Brunswick, NJ
. Maintained, validated and operated Illumina Sequencing Platforms.
. Planned and executed special projects
. Collaborated directly with principal investigators (PI's) and internal
post doctoral students
. Instructed internal and external post doctoral students in sequencing
protocols
. Assisted in the implementation of the university's inaugural work in
algal single cell sequencing
. Consulted with PI's on project logistics, feasibility, and methodology
. Conducted extensive research on DNA/RNA sequencing, which included
testing cutting edge techniques
. Completed lab work that culminated in the receipt of NIH grant funding
. Edited and formatted research for publication
. Published multiple articles in top tier journals within the field
AREAS OF EXPERTISE:
Expertise with model organisms: Caenorhabditis elegans, chlamydomonas
reinhardtii,
Porphyridium cruentum - functional genomics, pathology, neurobiology,
cell death, ROS production, DNA and nuclear fragmentation
Technical expertise: Next Generation Sequencing (Illumina); complete
understanding and operation of the HiSeq2500,MiSeq,HiScan, cBOT and
PEM (Paired End Module) including software SCS and SAV; Extraction of
DNA and RNA for preparation of various generations of Next Generation
Libraries including Multiplexed Transcriptomic, Exome, Genomic, Mate
pair, PCRfree, Small RNA, and Degradome Libraries; Validation,
quantification and quality control of Illumina Libraries via
Bioanalyzer, Fragment Analyzer and qPCR
Electrophoresis: Agarose gel electrophoresis
Spectroscopy: UV - visible, plate reader
Microscopy: Confocal microscope, fluorescent microscope, phase-contrast
microscope, epifluorescence microscope, stereo microscope
Microbiology: growing microbes under aseptic conditions, media
preparation and autoclaving, serial dilutions, plating/streaking
Cell biology: Cell biological studies in C. elegans by using phase
contrast, fluorescence and confocal microscopes
Computer skills: Excel, Power Point, Adobe Photoshop, Endnote,
Integrative Bioinformatics Databases like NCBI and LIMS
[pic]
Divino V. Rajah