Manager Assistant

RENU JOSEPH

**** ******** **** ** *******, TX ***82 Phone: 225-***-****; E-mail: *********@*****.***

Permanent Resident of USA. No employment authorization is required

PROFESSIONAL WORK EXPERIENCE:

Research Associate (2011- August 2012), Ochsner Clinic Foundation, Institute for Translational Research, Jefferson, LA

• RT- Quantitative Real-Time PCR for studying the expression profiles of various genes of interest, analysis of data using excel software

• Cell Culture and transfections with plasmid constructs of interest for downstream applications

• Immunohistochemistry (single, double staining), Immunocytochemistry and image analysis by 3-D Deconvolution/Fluorescence microscopy- Studied the histological changes in various organs/tissues of diabetic and hypertensive transgenic and wild type mouse

• DNA/RNA Isolation, PCR, Southern Blotting- genotyping transgenic and wild type mice of interest

• Animal husbandry- manage transgenic and wild type mouse colony, including mouse databases, breeding, weaning, tagging, injections, sample collection, genotyping, euthanasia and minor surgical procedures

• Western Blotting- for protein expression studies

• Monitor spending of grants and funds

• Taking classes and setting up lab experiments for STAR (Science Technology And Research- summer outreach program) for school students

Research Associate / Lab Manager (2008 –2011) LSU System (Pennington Biomedical Research Centre), Baton rouge, LA

• DNA Isolation, PCR, Southern Blotting- genotyping transgenic mice

• Gene Expression Assays- The expression profiles of genes related to obesity utilizing Real-Time PCR, Low Density Array (Taqman) method in low fat/high fat diet /drug treated mouse tissues

• Primary Cell Culture- Isolation, propagation and storage of Ear Mesenchymal Stem cells (EMSC) from mouse and working with various cell lines

• Bisulfite conversion & sequencing- to investigate the variability in MEST (Mesoderm Specific Transcript) gene expression in mouse adipose tissue due to difference in methylation pattern

• DNA Hypersensitivity Mapping- Investigated the regulatory elements of obesity associated genes with DNA Hypersensitivity mapping employing Real- Time PCR

• Lab management including maintaining inventories of supplies, preparing and approving orders and service contracts, equipment purchase and maintenance, maintaining lab databases

• Trained 4 under graduate student workers in nucleic acid isolations, PCR, Real- Time PCR, cell culture etc

Research Assistant (2005–2007), Department of VMP, Washington State University, Pullman

• Determined antimicrobial resistance genes responsible for the resistance phenotype in Multi-Drug Resistant E-coli isolates employing PCR

• Identified the pattern of shared resistance genotypes in streptomycin sulphonamide tetracycline (SSut) and Multi Drug Resistant E-coli isolates

• Detected the presence of plasmid encoded resistance in Multi Drug Resistant isolates in E-coli by electroporation and plasmid profiling experiments

• Bacterial cell transformations, Plasmid Profiling using Kado & Liu method for determining presence of plasmid DNA, plasmid electrophoresis, Gel electrophoresis

• Worked with pathogenic and non-pathogenic microbes in BSL-2/3 labs.

• Making electrocompetant cells (E.coli DH10B) for electroporation of plasmid DNA

EDUCATION

• 2007– MS in Veterinary Microbiology, Washington State University, Pullman, Washington

• 2004 – DVM, Kerala Agricultural University, India

LABORATORY SKILLS

• Over 8 years of experience in primary cell culture of Ear Mesenchymal Stem Cells, working with various cell lines 3T3L1s, J774

• Over 8 years of experience in Gene expression analysis employing Real Time PCR, Low Density Array

• Extensive experience with the use of immunohistochemistry and subsequent image analysis to understand molecular and cellular processes involved in Renin-Angiotensin system

• Over 8 years of experience working with lab animals in research setting- breeding, genotyping, dissection etc of mice and rats

MOLECULAR TECHNIQUES

• Extensive experience in using PCR for gene detection/genotyping

• Over 8 years of experience with enzyme immunoassays (ELISA), Western Blotting including the performance, troubleshooting, and data analysis using excel, affinity purification of antibodies

• Making of DNA embedded agarose beads for bisulphite conversion and subsequent bisulphite sequencing

• Experience in TA cloning of PCR products into chemically competent bacteria

• Over 8 years of experience in using Real-Time PCR for gene expression analysis of obesity associated genes

• Southern Blotting, PFGE, RFLP, Protein isolation, TAQMAN PCR

• Extensive experience in isolation of RNA and DNA from mouse and rat tissues and cells for further downstream applications

• Experience working with Robotics- Multiprobe II Nucleic acid work station for PCR mass clean ups and setting up 384 well plate for Real- Time PCR

• Knowledge of Sequencher, Mutation Surveyor

MICROBIOLOGICAL TECHNIQUES

• Handling and processing of tissue samples, hands on experience in the preparation of microbial cultures and routine microbiological laboratory techniques

• Detection, Isolation, culture identification, genotyping and characterization of microbes

COMPUTER SKILLS

• SAS, MINITAB, BLAST, Vector NTI, MS Word, Excel, Power Point and Internet databases

PRESENTATIONS, HONORS and AWARDS

• Invited talk, Pioneer Hi-Bred Johnston, Iowa, March 19 2010

• Graduate Research Assistantship, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 2005-2007

• KVPY scholarship, Indian Institute of Science, Bangalore, India, for project “Seroprevalance of Brucellosis in Slaughtered cattle in and around Thrissur”,2001

• Kerala State Scholarship for DVM 1998- 2004

• National Merit Scholarship for outstanding student 1995-1997

COURSE WORK:

Advanced Molecular Biology, Population Genetics, Biochemistry, Statistics, Physiology, Anatomy, Clinical Medicine, Preventive Medicine, Epidemiology, Obstetrics and Gynaecology, Pharmacology, Nutrition

VETERINARY CLINICAL EXPERIENCE

• Provided full time diagnosis and treatment for small and large animals while working as a clinical intern in Government Veterinary Polyclinic, Angamaly, India (2004)

• Worked as a Field Veterinarian in Aluva Government Veterinary clinic, India (2004)

• Involved in several vaccination programs conducted by the state government in India against FMD, BTV, HS, ET etc, conducted epidemiological surveys of zoonotic diseases of public health importance, through several extension programs

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