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Engineering Assistant

Location:
Piscataway Township, NJ
Posted:
November 12, 2013

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Resume:

Kevin Thompson

*** **** **** **** **** ***

Piscataway, NJ 08854

908-***-****

******@*****.***

Summary Neuroscientist Ph.D. with biomedical engineering bachelor’s and broad research experience in molecular

and cell biology including 3 years undergraduate, 1 year lab tech, 4 years of graduate level experience.

Additionally, teaching, mentoring, manufacturing and leadership experience.

Education Neuroscience Ph.D. defense date December 11, 2013 GPA: 3.8

UMDNJ/Rutgers University Graduate School of Biomedical Sciences, Piscataway NJ

Thesis HuR regulates cell cycle and translation in neocortical neural stem cells

* Studied posttranscriptional regulation of neurogenesis

* Implemented conditional genetic deletion scheme to ablate HuR in neural stem cells

* Used advanced techniques to analyze structural and molecular phenotypes associated with HuR deletion

* Investigated secreted signaling molecule regulation of neurogenesis

* Mentored 9 undergraduate students through summer research programs and research for credit

* IGERT Fellow September 2010-2012

* Oral and poster presentations both on and off campus

Rutgers University School of Engineering, Piscataway NJ

Bachelor of Science in Biomedical Engineering, May 2008, GPA: 3.9

Thesis Controllable Notch expression for embryonic stem cell to neural stem cell differentiation

* Created embryonic to neural stem cell differentiation platform

* Developed lentiviral delivery system for controllable Notch expression

* Developed recombinant Notch protein modified with a basic amino acid sequence for direct

transduction of Notch across cell membranes

Experience Stem Cell Research Center, Rutgers University, Piscataway NJ

Lab Technician, June 2008 – September 2009

* Assisted is design and construction of molecular tools for pluripotent stem cell generation

* Assisted in development, maintenance and culture of immortalized, primary and embryonic stem cells

* Assisted in training of industrial and academic individuals through a stem cell training course

Department of Biology, Rutgers University, Piscataway NJ

Teaching Assistant, September 2012 – May 2013

* Undergraduate level General Biology laboratory instructor

* 2 sections of 3-hour lab classes per week including a 1-hour instructional lecture

* Provide individual support for students seeking additional instruction

Lab skills

Molecular cloning Polysome Fractionation

Lentiviral generation Luciferase Assay

Cell culture Cell Biology

Recombinant protein synthesis/isolation Confocal Microscopy

Infection/Transfection/Transformation Stem Cell Biology

RNA/DNA/protein isolation Biochemistry

qPCR / PCR Teaching/Mentoring

Protein IP Animal Husbandry

Western Blot Genetics

Manuscripts in Preparation

Specification of neocortical neuron subpopulations by timed thalamic WNT3 regulation of HuR-dependent post-

transcriptional events

Kevin Thompson, Barbara Viljetic, Kristina Sakers, Vera Medvedeva, Joseph D. Dekker, Philip W. Tucker, Dimitris L.

Kontonyiannis, Matthias Groszer, Ronald P. Hart, Mladen-Roko Rasin

HuR interacts with the initiation kinase eIF2ak4 to regulate ribosomal assembly and translation

Thompson K*, Kraushar ML*, Wijeratne S, Viljetic B, Sakers, K, Kontonyiannis DL, Hart R, Rasin MR

*equally contributed

Developmental heterogeneity of the neocortical ribosome is regulated by timed WNT3 secretion from in-growing

thalamocortical axons

Matthew L. Kraushar, Kevin Thompson, H.R.Sagara Wijeratne1, Barbara Viljetic, Ronald P. Hart, Mladen-Roko Rasin,

Publications

Thompson K., DiBona L.V., Dubey A., Crockett P.D., Rasin MR. (2010) Acute adaptive responses of central

sensorimotor neurons after spinal cord injury. Trans Neur. 1(4) 268-278.

Moore J.C., Atze K., Yeung P.L., Toro-Ramos A.J., Camarillo C., Thompson K., Ricupero C.L., Brenneman M.A., Cohen

R.I., Hart R.P. (2010). Efficient, high-throughput transfection of human embryonic stem cells. Stem Cell Res Ther. Jul

26;1(3):23.

Li H., Han Y.R., Bi C., Davila J., Goff L.A., Thompson K., Swerdel M., Camarillo C., Ricupero C.L., Hart R.P., Plummer

M.R., Grumet M. (2008) Functional differentiation of a clone resembling embryonic cortical interneuron progenitors.

Dev Neurobiol. Dec 68(14):149-64.



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