Assistant Medical
Resume:
APARNA PATHAK
** ******** ******, *** *, Somerville, MA 02144
Cell Phone 617-***-**** Email: **************@*****.***
TECHNICAL EXPERTISE
Immunology Skills:
• ELISA: Direct, Indirect binding assays, antibody titer determination
• Flow Cytometry – HTP Guava easyCyte, BD FACS Aria III
• Cell based assays
• ELISPOT
Tissue Culture Skills:
• Adherent and Non-adherent Mammalian Cell Culture
• Types of cells handled: CHO, HEK-293, NIH-3T3, Primary murine cells.
Automation Skills:
• Sciclone ALH 3000 Caliper, Tecan Freedom evo 150
• Eppendorf epmotion 5075
• BenchPro, BioTek Plate washer
Animal Handling Skills:
• Transgenic Mice handling, dissection, harvest organs, Genotyping
• Immunizations: sub-cutaneous and intra venous injections
Molecular Biology Skills:
• Transfection, Cloning, Mini and Maxi Prep using Qiagen Kits.
• DNA and RNA Isolation, RT-PCR, PCR
Additional Laboratory Skills:
• SDS-PAGE, Western Blot, HPLC, Fluorescent microscopy, Agarose gel electrophoresis,
nanodrop
Computer Skills:
• Microsoft Office, GraphPad Prism, SoftmaxPro, Chemstation for Agilent HPLC systems.
EXPERIENCE
EMD Serono Research Institute, Billerica, MA
Research Assistant Co-op
July 2013-Present
• Currently working in B-Cell group of Protein Engineering & Antibody Technologies department,
investigating early stage large biological molecules.
• Develop new ELISA assays to validate reagents for new discovery projects.
• Determine serum antibody titer from immunized mice.
• Screen and identify potential binders against different antigens using ELISA in a 96 well plate
format.
• Execute high-throughput antibody or hybridoma screening in-vitro, on cells expressing the target
using flow cytometry. Instrument: Guava easyCyte 6HT
• Perform ELISPOT to detect certain B-cell secreting antigen specific antibodies.
• Assisted in sorting of B-cells using BD FACS Aria III.
• Utilized laboratory automation like Sciclone ALH3000, Tecan evo 150 & epMotion 5075 to
increase throughput.
• Assist in technology and method development to support new antibody discovery projects.
• Maintain, passage and cryopreserve stable and transient transfected cell lines.
• Determine the cell density and viability using the Vicell-XR automated cell counter.
• Analyze data; interpret results and document in an electronic laboratory notebook (ELN).
• Prepare buffers, growth medium and maintain reagent inventory.
• Follow company safety guidelines, GLP regulations and standard operating procedures.
Tufts Medical Center, Boston, MA
Research Assistant Intern
April 2012-April 2013
• Investigated the role of CDK6 in adipogenesis on different strains of transgenic mice
• Isolated mesenchymal stem cells and mouse embryonic fibroblasts, carry out cell culture
experiments. Sub-culture and maintain different cell lines for further analysis and staining.
• Bred, maintained and genotyped mice. Isolated DNA from different mouse tissues and perform
PCR to amplify the specific gene fragments, followed by Agarose gel electrophoresis.
• Studied RNA expression of cells under experimental conditions by RT-PCR
• Executed out small-scale and large-scale plasmid DNA isolation from bacterial cells.
• Used lentivirus vectors to genetically modify cells, expand the gene modified cells in culture.
• Use flow cytometry-based assays to characterize cells.
• Use cell-based assays to assess the functional characteristics of genetically modified cells.
EDUCATION
Northeastern University, Boston, MA December 2013
Master of Science in Biotechnology (Pharmaceutical Track)
Dr. D.Y. Patil University, New Mumbai, India July 2011
Bachelor of Technology in Biotechnology
PUBLICATION
CDK6-mediated repression of CD25 is required for induction of T cell acute lymphoblastic
leukemia by activated Notch
Authors: Nilamani Jena, Jinghao Sheng, Jamie K. Hu, Wenhui Zhou, Nicklous Tsichilis, Aparna Pathak,
Nelson Brown, Amit Deshpande, Chi Luo, Gene Lee, Guo-fu Hu, Philip W. Hinds, Richard A. Van Etten
and Miaofen G. Hu (In press)
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