Anatoly Mayburd

**** ***** ***** *****, **. ***3, Alexandria, VA 22303. 240-***-****.

aca7eo@r.postjobfree.com

SUMMARY

. 17 + years of research in Molecular Biology, Chemistry,

Computational Biology and Chemical Engineering.

. First author of peer reviewed publications in molecular biology

and nuclear engineering, pending patent applications in infometrics and

nuclear engineering

. PhD in Molecular Biology, NIH/NCI post-doctorate, Bachelor in

Chemical Engineering.

. Adjunct Faculty of the System's Biology Department of George

Mason University.

. Extensive and diverse technical background combined with

technical and organizational creativity.

. Skilled in multiple tools of data mining, analysis and

statistical/mathematical modelling, emergency situation modelling and

response planning.

. Registered Patent Agent.

. US citizen, clearable.

. Knowledge of Russian language and culture, experience of working

abroad.

. Performance-related bonuses, Certificates of Excellence issued by

the current and past employers.

. DTRA Golden Star Award

TECHNICAL SUPPORT EXPERIENCE

J & A Research, Alexandria, VA

Patent Agent, May 2012 - Present

. Research and analysis

Conducting patent-related legal services for firm clients (application

drafting and filing, help in opinion preparation, PCT application

examinations). Patent, trademark and legal searches. competitive

intelligence, infringement, validity and patentability projects, technology

reports. Apply Thompson Innovation, EAST and Patbase tools of patent

research, STN MARPAT structure searching, STN and GenomeQuest sequence

searching, Thomson Reuters, Westlaw Next, Hoovers, Mintel, Global Data

Pharma eTrack, Frost & Sullivan tools of legal and business research, Nexis-

Lexis, Factiva, Business NewsWire, Forbes.

Access stock market data, merger and acquisition data.

Produce competitive landscape analysis, supplier and client analysis using

technology landscape data, White Space analysis.

. Technologies and technical leadership

The technologies include chip design and fabrication. Communications,

signal processing, semiconductor design, web development, medical devices,

bio-products, pharmaceuticals, therapeutics, foodstuff and medical

compositions, petrochemistry and petroleum engineering, chemical and

nuclear engineering, electrochemistry and fuel cells, energy production,

general machinery, automation, sensors, material science, business methods

and software, electric circuits, solid-state physics, data processing.

George Mason University, Fairfax, VA

Adjunct Faculty, November 2012 - Present

. Research and analysis

Honorary unpaid position enabling collaboration with GMU scientists in the

System's Biology research and inventorship, access to GMU library.

Landon-IP, Washington, DC

Bilingual Patent Analyst, January 2011 - May 2012

. Patent search and analysis

Working in diverse technical fields, provided a steady revenue stream of 30

K per a month.

Conducted PCT Article 18 examinations on international patent applications

at the request of International Search Authority (ISA), established

International Search Reports by PCT Article 43. The examination included

evaluation of the issues of patentability, invention description and claim

support, new matter, utility, invention unity, formalities etc. Performed

Accelerated USPTO Examination, Competitive Landscape, Freedom-to-Operate,

State-of-the-Art, Infringement, Validity, Opposition, Patentability

searches of prior art. STN sequence and structure searching.

. Litigation support

Reviewed technical references to search for possible invalidity positions

or evaluated opposing party's invalidity positions. Prepared claim charts

for non-infringement and invalidity positions. Played a decisive role in

providing critical prior art to support the needs of the company's clients,

serving lawsuits up to 100 million worth.

. Patent drafting

Drafted provisional and non-provisional patent applications, including

detailed descriptions, claims, and drawings of patent applications.

. Technologies and technical leadership

The technologies included medical devices, bio-products, pharmaceuticals,

therapeutics, foodstuff and medical compositions, chemical and nuclear

engineering, electrochemistry and fuel cells. Enhanced reputation of the

company by scientific research in the field of expertise, became a "go-to"

technical lead. Filed a patent application US 13/067,798 on June 28, 2011,

currently pending, titled "Methods of pulsed nuclear energy generation

using piston-based systems". Explored and published a treatise on a

possibility of miniaturized fission-fusion hybrid reaction core with

positive fusion yield in "Nuclear Engineering and Design", Volume 241,

Issue 1, January 2011, Pages 59-66.

CPA Global, Alexandria, VA

Senior Patent Researcher, March 2007 - January 2011

. Prior Art Search

Working in diverse technical fields, provided a steady revenue stream of 20-

25 K per a month. Performed Accelerated USPTO Examination searches,

Technological Landscaping, Freedom-to-Operate, State-of-the-Art,

Infringement, Validity, Opposition, Patentability searches of prior art.

Conducted STN structure searching. Was able to find prior art references

suitable for invalidating patents under 35 U.S.C.102 and 103 in most cases.

. Litigation support

Reviewed technical references to search for possible invalidity positions

or evaluated opposing party's invalidity positions. Prepared claim charts

for non-infringement and invalidity positions. The scale of lawsuits

reached up to $1 bln. Played a decisive role in providing critical prior

art to support the needs of the company's clients, bringing new business.

Travelled abroad and worked in foreign patent offices to procure the needed

evidence.

. Technologies and technical leadership

The topics of patent research comprised medicine and medical devices,

therapies, diagnostics, biology related computational methods,

pharmaceutical compositions, stem cell methodologies, chemical processes,

composite materials and organic synthesis, petrochemistry, manufacturing,

mechanisms and machine parts, separations and purification, fuel and

electrochemical cells, algorithms, business methods and more.

Was continuously enhancing the company's reputation by publishing

original (non-patent) research and by producing inventions. The

publications were used by sale's team to attract new business.

In 2008-2010 developed a novel method of IP landscaping analysis,

assessing a probability for a given technological sector to yield a block-

buster commercial product. The topic of the landscape studies were

countermeasures against pandemic influenza and small-pox. The method may be

useful for placement of venture capital and approval of grant proposals.

The method was published as invited reviews in PRI journal (see below the

list of references). The invention was patented by the employer (see

below).

Northrop Grumman/DTRA, Ft. Belvoir, VA

Senior Reachback Engineer IV, March 2006 - March 2007

. Consequence assessment and computational disaster modelling

Conducted 24/7 computational support of Army/National Guard WMD/natural

disaster preparedness training, with the focus on chemical and biological

warfare. Facilitated Army WMD preparedness training exercises by answering

the technical questions of military personnel in peaceful and combat

conditions. Conducted a live urgent analysis of real situations using

encrypted lines, was awarded DTRA Golden Star. Conducted writing of

technical scenarios of potential WMD attacks and proposed countermeasures.

The scenarios were well accepted by DTRA colleagues and by Civil Support

Team personnel and were later used in the preparedness training.

. Technical leadership

Conducted a research on the possibility of creating supra-miniature hybrid

fissile matrices, capable of supporting a controlled fusion component. The

results were later published by the NED journal (Nuclear Engineering and

Design). The material of the article was filed as a provisional patent

application with USPTO in 2011. Conducted a research of existing

countermeasures against pandemic infections and passed field training in

identification of the pathogens by rapid PCR.

. Additional duties

Performed additional duties as a substitute training program coordinator.

RESEARCH EXPERIENCE

National Cancer Institute, Bethesda, MD

Post-doctoral fellow, October 2002 - March 2006

. Streamlining of preclinical drug design

Designed and performed a study of lung cancer producing a computational

method assessing a potential of a gene to express a successful anti-cancer

drug target, resulting in 6-fold enrichment in FDA-approved anti-cancer

targets at 50% recall rate. The method allowed 40-fold+ enrichment for anti-

microbial targets at 45% recall rate as well. Translational applications

based on gene scores produced by this method may facilitate development of

new therapies. These studies led to a still broader research into the

origin of chronic diseases and into general characteristics of a successful

pharmacological target, using integrated datasets of multiple sources.

Developed and employed customized microarray data processing plug-ins.

Focused on employing different mechanisms and techniques to extract and

subsequently assemble information from diversified biological data sources

for presentation to facilitate gene analysis. The methods mentioned above

were published in Bioinformatics Oxford, 2008 and in Plos One, 2009.

. Elucidation of a drug mechanism

Designed and conducted microarray study of MK886 drug effect upon lung

cancer cell lines and transgenic mice. MK886 study resulted in a proposed

novel mechanism of anti-cancer activity with high potential of developing

synergistic and super-additive drug and agent combinations. The findings

were published in Clinical Cancer Research, 2006 and Cancer Research, 2005

see below.

. Elucidation of a protein function

Designed and conducted a study aiming at understanding of biological

function of FLAP (5-lipoxygenase activator protein) and the pharmacological

mechanism of its antagonist MK-886 in lung cancer. The protein is the

upstream initiator of leucotriene biological pathway, important in cancer

development, inflammation, allergies, asthma, autoimmune and aging

diseases. Constructed cell lines with over-expressed FLAP protein level and

demonstrated increased sensitivity to MK886. Additional role of FLAP as an

apoptotic perinuclear trigger was proposed and presented at NIH Research

Festival, 2004.

Rush University, Chicago, IL

Post-doctoral fellow, July 2001 - August 2002

Designed and performed a study of conformational changes in viral fusion

proteins of influenza HA and HIV gp41 classes supporting penetration of the

cellular outer membrane by a viral particle. Understanding of their complex

conformational dynamic is important for design of novel inhibitors.

Proposed FRET-based assay of 6-helix bundle-cleaving ligands as a potential

novel sensitive screening method for anti-influenza drug candidates.

Acquired valuable understanding of molecular basis of influenza and HIV.

University of Illinois, Chicago, IL

Doctorate Student, October 1994 - March 2001

The project was focused on establishing of a biological function of a

bacterial protein cytochrome c prime, found in a number of anaerobically

growing pathogens.

Applied research techniques such as oxygen-free gas phase titrations, stop-

flow kinetics,

reconstitution experiments, binding constraint extraction, point

mutagenesis and bioinformatic analysis. The results were published in

Biochemistry, 2002, assigning to cytochrome c prime the biological function

of a nitric oxide shuttle.

In the process of working with a gel marker dye BPB its high affinity

binding to cytochrome C prime was discovered and Rasmol analysis of the

protein X-ray crystallization data uncovered an extensive hydrophobic patch

on its surface. BPB turned out to be a useful spectral probe for detection

of active sites in enzymes and the results were published in Analytical

Biochemistry, 2003.

TECHNOLOGIES USED AS A RESEARCHER

As an enthusiastic practitioner of state-of-the-art computing technologies,

I had accrued in-depth hands-on professional experience in the following

areas:

. Bioinformatics

BLAST, Multiple Sequence Alignments, Genome Projects, SNP databasess,

Pathway reconstruction using Ingenuity Pathway Assistant, SwissProt, KEGG,

BRENDA enzyme and pathway annotation tools, OMIM, Gene Ontological

analysis, Phylogenetic data extraction and manipulation, Protein viewers

and surface study, VAST homology tool, Literature-extracted gene lists by

multiple sources, Affymetrix microarray primary analysis (MAS5, MadBee

system), Sharing with public domain via GEO uploads and downloads, Data

processing using Partek Pro and Madbee, Integration of microarray data

from multiple platforms, Clustering, Principal Component Analysis and

Classifier building, Drug Bank and Pubchem, NCI-60 panel usage and drug

screening.

. Modelling and optimization

Response surface construction and "black-box" optimization of experimental

systems. Construction of prognostic classifiers. Data fitting, extraction

of important parameters from linear and non-linear regression models.

Modelling of emergency situations and approximation of the response effort

using CBRNE tools (HPAC, CATS).

. Methods and tools of Homeland security

Integrated use of HPAC4.04R, CATS v.1 and v.6, ArcGIS9.1 and other

specialized tools of consequence assessment in CBRNE analysis. Sample

collection, preparation and field identification of biological warfare

agents using glass capillary/rapid PCR, statistical assessment of the data

and integration of the field results with analytical software output.

. Technical information research

Skilled in comprehensive research of technical literature by applying end-

user (PatBase, EAST, Micropatent, Delphion, Thompson Innovation) and

integrated databases, including chemical structure such as Questel, Merged

Markush Service (MMS), TOPGRAG structure drawing interface, STN. Extract

merger, acquisitions and new product announcements using DIALOG by Thomson.

Conduct sequence searching using STN and GenomeQuest. Expertly leverage

the resources of the Library of Congress and of the National Library of

Medicine.

. Standard methods of Molecular Biology

Real-time PCR, Flow cytometry and confocal microscopy, FRET fluorescent

labeling technique, two-dye cell fusion assays, fluorescent microscopy,

immunodetection (FACS, ELISA, immunoprecipitation, antibody labeling, Fab

production), Standard culturing of primary and malignant cell lines, In-

vitro reconstitution experiments, siRNA treatment of the drug's target

protein's transcript, Point mutagenesis and vector construction,

Purification and screening of potential partners for a target protein

utilizing volume exclusion, ion exchange, hydrophobic chromatography,

affinity column construction, and iso-electric focusing, designed in-vitro

reconstituted reporting system from purified bacterial components. Mobility

shift assays in non-denaturing acrylamide and agarose gels, Yeast Two-

hybrid system I for detection of the interaction partners of cytochrome c';

Culturing bacteria other than E. coli, Ligand binding and quick kinetic

(stop flow) studies under both aerobic and anaerobic conditions, analysis

of binding and kinetic data by SigmaPlot, fitting and extraction of

affinity constants, and comparison of alternative binding models.

TEACHING AND TRAINING EXPERIENCE

Taught General Chemistry 101, 112, 114, 116, Organic Chemistry 232, 234,

Biochemistry 354, Physical Chemistry 223, 224, Independent research 450,

both classes and labs, mentored the interns and junior lab personnel in

Standard Techniques and safety.

EDUCATION

University of Illinois at Chicago, Chicago IL

Philosophy Doctor in Biochemistry

MHTI named after Mendeleev, Moscow Russia

B.S. Chemical Engineering, Major GPA 4.5/5.0

ADMISSIONS

U.S. Patent and Trademark Office

AWARDS

DTRA Golden Star, 2006, Performance-related bonuses 2007-2010, Certificate

of Excellence by CPA, 2008 and Landon-IP in 2011

AFFILIATIONS

PIUG (patent information users group), ACS (American Chemical Society),

Review Board of Bioinformatics.

PERSONAL

Fluent in Russian

REFERENCES

References, transcripts and writing samples are available upon request.

APPENDIX

. Patents

PCT/US10/58667 filed on December 1, 2010, currently pending, titled

"Methods and systems for performing analysis on documents related to

different technological fields".

US 13/067,798 filed June 28, 2011, currently pending, titled "Methods of

pulsed nuclear energy generation using piston-based systems".

DHHS ref. no. E-268-2005/0-US-01, M&G ref. no 11613.76USP1

filed March 31, 2006, currently abandoned, titled "Methods and systems for

identifying and classifying drug targets".

. Publications

1: Mayburd AL et al. Smallpox antiviral therapeutics. Recent Patent

Antiinfect Drug Discovery 2011, Volume 5, number 4, pp 240-256, a solicited

review.

2: Mayburd AL. A possibility of miniaturized fission-fusion hybrid reaction

core with positive fusion yield. Nuclear Engineering and Design, Volume

241, Issue 1, January 2011, Pages 59-66.

3: Mayburd AL. Influenza antiviral therapeutics. Recent Pat Antiinfect Drug

Discov. 2010 1;5(1):64-75, a solicited review.

4: Mayburd AL. Expression variation: its relevance to emergence of chronic

disease and to therapy. PLoS One. 2009 Jun 15;4(6):e5921.

5: Mayburd AL, Golovchikova I, Mulshine JL. Successful anti-cancer drug

targets able to pass FDA review demonstrate the identifiable signature

distinct from the signatures of random genes and initially proposed

targets. Bioinformatics. 2008 Feb 1;24(3):389-95. Epub 2007 Oct 8.

6: Mayburd AL, Martl nez A, Sackett D, Liu H, Shih J, Tauler J, Avis I,

Mulshine JL. Ingenuity network-assisted transcription profiling:

Identification of a new pharmacologic mechanism for MK886. Clin Cancer Res.

2006 Mar 15;12(6):1820-7.

7: Avis I, Mart nez A, Tauler J, Zudaire E, Mayburd A, Abu-Ghazaleh R,

Ondrey F, Mulshine JL. Inhibitors of the arachidonic acid pathway and

peroxisome proliferator-activated receptor ligands have superadditive

effects on lung cancer growth inhibition. Cancer Res. 2005 May

15;65(10):4181-90.

8: Bertsch M, Mayburd AL, Kassner RJ. The identification of hydrophobic

sites on the surface of proteins using absorption difference spectroscopy

of bromophenol blue. Anal Biochem. 2003 Feb 15;313(2):187-95.

9: Mayburd AL, Kassner RJ. Mechanism and biological role of nitric oxide

binding to cytochrome c'.

10: Mayburd AL, Tan Y, Kassner RJ. Complex formation between Chromatium

vinosum ferric cytochrome c' and bromophenol blue. Arch Biochem Biophys.

2000 Jun 1;378(1):40-4.

11: Mayburd AL, Baranova A. Compact model of disease (under consideration

by Plos One journal).

Contact this candidate