Bio-Analytical Scientist
Resume:
NIRMAL PALIWAL
** ***** **** ******, *******, MA 01810
*.*.*******@*****.***
SUMMARY
Analytical Biochemist with characterization and method development
experience for variety of proteins like monoclonal antibodies, fusion
proteins, hormones, enzymes as well as peptides. Hands-on experience with
Mass Spectrometry (intact mass, subunit assay, peptide mapping, PTM
analysis), HPLC (RP, NP, IEX, SEC, Affinity, HIC, HILIC, HPAEC-PAD),
Capillary electrophoresis, SDS-PAGE, IEF, Enzyme Digests, CD, GC,
UV/VIS/Fluorescence spectroscopy, DLS, DSC, SPR, Cell Culture and Elisa.
Strong ability to work in a team as well as independently. Excellent
structural characterization and data interpretation skills.
EDUCATION
. Ph.D. in Biochemistry (Sep 2006 - May 2013)
University of Massachusetts Lowell, MA
. M.S. in Biomedical Engineering and Biotechnology (Sep 2006 - Dec
2008)
University of Massachusetts Lowell, MA
. B.S. in Pharmaceutical Technology (Jun 1998 - July 2002)
Marathwada University, Aurangabad, India
WORK EXPERIENCE
. Biogen Idec Inc, Cambridge, MA, USA (July 2013 - Present)
Scientist, Analytical Development (Contract position)
. Primary responsibilities include bio-analytical method
development and characterization of proteins and their post
translational modifications (PTMs)
. Use of analytical techniques like Capillary electrophoresis,
HPLC/UPLC, mass spectrometry (MS) and tandem mass spectrometry
(LC-MS/MS)
. Working simultaneously on multiple projects, both as a part of a
team and independently and drafting technical development
method protocols
. University of Massachusetts Lowell (Sep 2010 - May 2013)
Graduate Research Assistant
. Worked in Protein Analysis and Characterization laboratory
. Responsibilities included characterization of therapeutic
proteins using HPLC (RP, NP, IEX, SEC, Affinity, HIC, HILIC,
HPAEC-PAD), Biacore (SPR), SDS-PAGE, CD, IEF, Cell based assays
and ELISA for start-ups/virtual biotech partners
. PPD Dermatology Inc, Waltham, MA, USA (May 2009 - Jan 2010)
Research Specialist
. Worked in pre-clinical drug discovery team involved in screening
compound libraries against Inflammatory disease targets and Hit
generation
. Responsibilities included cell line development and performing cell-
based assays to evaluate gene transcription, protein expression,
protein purification using HPLC (RP, IEX, SEC, Affinity
Chromatography) based methods
. Further duties were viability and cyto-toxicity assays, Elisa and
multiplex cytokine assays
. On Assignment Inc, Cambridge, MA, USA (Aug 2008 - April 2009)
Research Associate
. Member of a team involved in Target validation and High throughput
screening (HTS) of compounds (siRNA's and small molecule controls)
. Duties included Gel Electrophoresis, Western blotting and
Microscopy based imaging assays
. Additional responsibilities were carrying out Cell based assays
like Proliferation Assay, Apoptosis Assay, Metabolic assays,
Multiplex assays (bDNA assay and cytokine assay) for mRNA as well
as protein expression level determination
. Millipore Corporation, Danvers, MA, USA (Jun 2007 - Jan 2008)
Summer Intern/Co-op
. Worked on a project evaluating Extracellular Matrix (ECM) and
coated cell culture ware
. Responsibilities for this position included culturing different
cell lines and assisting in the different evaluation assays
(viability and functional assays)
TECHNICAL SKILLS
. Reverse phase chromatography (RP), Normal phase chromatography (NP),
Size exclusion chromatography (SEC), Ion exchange chromatography(IEC),
Affinity chromatography (Protein A), HPAEC-PAD, Peptide mapping, Mass
spectrometry (LC-MS and LC-MS/MS), Carbohydrate Analysis, Biacore
(SPR), CE, CD, gel electrophoresis (SDS PAGE), IEF, DLS, Gas
chromatography, Cell Culture, UV- Visible spectrophotometers,
Western, Southern and Northern Blots, PCR/qPCR and Multiplex High
Throughput luminex Assays
. Knowledge of cGMP, GLP, ICH guidelines
. Proficient with Empower, Analyst, MassLynx and Xcalibur softwares
AWARDS AND PUBLICATIONS:
. University Fellowship Award
For the highest qualified entering doctoral student (June 2010).
. Group author research article titled, "Glycoprotein Ib alpha: an
intrinsic promoter of A? fibrillization". Authors: Tyler Carter,
Nirmal Paliwal and Jin Xu. Submitted to Journal of Clinical
Investigation (2013).
. First author research article titled, "Structural and functional
characterization of n-glycans from recombinant human lactoferrin
expressed in Aspergillus Awamori". Authors: Nirmal Paliwal, Tyler
Carter, and Jin Xu. Submitted to Journal of Glycoconjugate (2013).
.
REFERENCES
Available upon request[pic]
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