Clinical/Medical Scientist

Owusu CV, *

Curriculum Vitae

SARAH A. OWUSU, PhD

*** ********* **, ***************, ** 22901

Day Time Phone: 708-***-**** ● Email: ************@*****.*** LinkedIn: https://www.linkedin.com/in/sarah-a-owusu-phd-22901/ EDUCATION

Doctor of Philosophy in Physiology 2015

Pennsylvania State University, University Park, PA Bachelor of Science in Physiology 2009

Southern Illinois University, Carbondale, IL

CERTIFICATIONS

Advanced Certificate in Clinical and Translational Investigation 2018 Weill Cornell Medical College, New York, NY

College Teaching Certificate

The Pennsylvania State University, Schreyer Institute for Teaching Excellence 2013 WORK HISTORY

NIH T32 Postdoctoral Clinical Research Fellow, Department of Genetic Medicine 2016-2018 Weill Cornell Medical College – Cornell University; Advisor: Dr. Ronald G. Crystal Projects:

1. Cell Specificity and Smoking Modulation of Human Airway Epithelium Expression of ADH7, the Rate-Limiting Enzyme for Generation of Retinoic Acid

2. Antioxidant protection of the human airway epithelium through delivery of gene therapy of modified SOD3 and catalase

3. AGR2 as a therapeutic target for chronic obstructive pulmonary disease (in collaboration with Boehringer Ingelheim in Germany)

Developed, planned, executed, and reviewed collaborative research projects with a team of clinicians, research scientists, and statisticians across disciplines and departments as a lead scientist for research projects in-house and internationally

Produced and ensured compliance of standard operation procedures (SOPs) for projects, scientific apparatus, and standard laboratory experiments

Communicated scientific research findings for written grants and oral presentations at international scientific meetings

Provided statistical analysis of large processed datasets derived from genomic sequencing

Utilized and directed allocation of patient airway tissue biopsies and airway epithelial cells for experimental or analytical use

Recruited and interviewed prospective post-doctoral candidates for post-doctoral associate positions

Trained and mentored lab-techs, residents, and postdocs in scientific techniques and oral presentations for overall career development

Built project collaborations through networking at scientific events and conferences

Technical expertise: cell culture, air-liquid interface culture, siRNA and plasmid transfection, immunohistochemistry, immunoblotting, PCR, qPCR, imaging, designing adeno-associated virus vector, RNA isolation and purification, protein isolation and purification, ELISA, analysis and utilization of large data sets derived from RNA-sequencing and microarray of human tissue biopsies and cell Owusu CV, 2

Graduate Student Researcher, Intercollegiate Graduate Degree Program in Physiology 2010-2015 Advisor: Dr. A. Catharine Ross Pennsylvania State University; Dissertation Project: Vitamin A Supplementation: Its Effects on Ontogeny, Lung Development, and Neonatal Lung Injury

Composed, submitted and received approval for an institutional animal care and use committee (IACUC) protocol.

Identified funding sources, acquired numerous monetary awards, and wrote successful grant proposals to generate over $100k+ in funding to support research projects

Trained, and mentored undergraduate students in basic scientific research and writing

Quickly grasped technical expertise in various techniques in order to develop and optimize a novel lung injury model for various projects in order to discover treatments

Kept record and track of experiments, data, and reports for individual and collaborative projects

Communicated scientific findings through presentations at international conferences and peer-reviewed scientific article publications

Technical expertise: PCR, qPCR, flow cytometry, Ultra Performance Liquid Chromatography, RNA isolation, fixing of tissues, paraffin embedding, and microtome tissue sectioning; immunohistochemistry, imaging, ELISA, lipid extraction, and designing primers Post-Baccalaureate Research Fellow, Department of Pathology 2009-2010 Post-baccalaureate Research Education Program (PREP) University of Alabama at Birmingham; Advisor: Dr. Victor Darley-Usmar Project: The Role of Mitochondria and Redox Cell Signaling with a Particular Emphasis on Hydrogen Peroxide and Caspase Activity

Recorded, analyzed, and reported research statistical findings

Presented research findings at the Biomedical Research Conference for Minority Students (ABRCMS)

Technical expertise: Seahorse Bioscience XF24-3 Extracellular Flux Analyze, cell culture, spectrophotometry, western blotting and imaging using film developer, chemiluminescence, and typhoon scanner; and protein purification

Undergraduate Student Research Assistant, Department of Physiology 2007-2009 Southern Illinois University – Carbondale; Advisor: Dr. Buffy Ellsworth Lab Project: The Expression of Foxd1 in the Pituitary During Embryonic Development

Trained undergraduate students and postdoctoral associates in scientific experimental techniques

Communicated research findings through a peer-reviewed publication and various scientific conferences/meetings

Technical expertise: PCR, genotyping, microtome and cryostat tissue sectioning, immunofluorescence and immunohistochemistry, and microscope image processing WRITTEN AND VERBAL COMMUNICATION EXPERIENCE

PUBLICATIONS

1. Owusu, SA, Wang G, Leopold PL, Salit J, Crystal RG. NRF2 Regulation of ADH7 in the Human Large Airway Epithelium. In preparation

2. Owusu, SA, Ross, AC. Retinoid Homeostatic Gene Expression in Liver, Lung and Kidney: Ontogeny and Response to Vitamin A-Retinoic Acid (VARA) Supplementation from Birth to Adult Age. Plos ONE 2016; Jan 5;11(1):e0145924. doi: 10.1371/journal.pone.0145924 [PMC4701161]

3. Ross AC, Tan L, Owusu, SA. 2016. Chapter 31, Vitamin A Metabolism in the Fetus and Neonate. In: Polin RA, Abman SH, Rowitch D, Benitz WE, editors. Fetal and neonatal physiology. 5th edition. Philadelphia (PA): Elsevier, Inc. p. 317 – 325.

4. Gumbel JH, Patterson EM, Owusu SA, Kabat BE, Jung DO, Summons J, Hopkins T, Ellsworth BS. The Forkhead Transcription Factor, Foxd1, is Necessary for Pituitary Luteinizing Hormone Expression in Mice Plos ONE 2012; Dec 19; 7(12): e52156. 10.1371/journal.pone.0052156 [PMC3526578] Owusu CV, 3

PRESENTATIONS

Scientific Presentations

1. “NRF2 Regulation of ADH7 Expression in the Human Airway Epithelium Occurs via both Retinoic Acid Receptor and KEAP1 Pathways” May 2018. Poster Presentation. American Thoracic Society International Meeting. San Diego, CA. 2. “Anatomic Gradients, Cell Specificity and Smoking Modulation of Human Airway Epithelium Expression of ADH7, the Rate-Limiting Enzyme for Generation of Retinoic Acid” May 2017. Poster Presentation. American Thoracic Society International Meeting. Washington, D.C.

3. “Vitamin A Supplementation: Its Effects on Ontogeny, Lung Development, and Neonatal Lung Injury” September 2015. Oral Presentation. Nutritional Sciences Department Colloquium Series. University Park, PA 4. “Vitamin A supplementation and ontogeny affect maturation-related gene expression in neonatal rat lung” November 2014. Poster Presentation. IGDP Physiology Retreat. University Park, PA 5. “Vitamin A supplementation and ontogeny affect maturation-related gene expression in neonatal rat lung” June 2014. Poster Presentation. FASEB 2nd International Retinoids Conference. Chicago, IL 6. “Ontogenic expression of retinoid homeostatic genes in principal vitamin A storage organ” May 2014. Penn State 7th Biennial Pediatric Research Day. Hershey, PA

7. “Vitamin A supplementation and ontogeny affect maturation-related gene expression in neonatal rat lung” April 2014. ASN VM-RIS Poster Competition. Experimental Biology Annual Meeting. San Diego, CA 8. “Vitamin A supplementation and ontogeny affect maturation-related gene expression in neonatal rat lung” April 2014. Oral Presentation. Experimental Biology Annual Meeting. San Diego, CA 9. “Ontogenic expression of retinoid homeostatic genes in principal vitamin A storage organ” September 2013. Oral Presentation. IGDP Physiology Retreat. University Park, PA 10. “Ontogenic expression of retinoid homeostatic genes in principal vitamin A storage organ” April 2013. Poster Presentation. Experimental Biology Annual Meeting. Boston, MA 11. “Vitamin A supplementation and dietary status influence the metabolism of retinoids during early postnatal development” March 2013. Poster Presentation. Penn State Graduate Exhibition. University Park, PA. 12. “Impact of mitochondrial function on cell death in the endothelium” February 2010. Oral Presentation. University of Alabama in Birmingham Graduate Student Research Day. Birmingham, AL. 13. “The expression of Foxd1 in pituitary during embryonic development” October 2009. Poster Presentation. The Annual Biomedical Research Conference for Minority Students. Phoenix, AZ 14. “The expression of Foxd1 in pituitary during embryonic development” June 2009. Poster Presentation. The Endocrine Society 91st Annual Meeting. Washington, DC. 15. “The expression of Foxd1 in the pituitary during embryonic development” October 2008. Poster Presentation. 17th Annual McNair Scholars Conference and Graduate Fair. Delevan, WI. Non-science Presentations

1. “Oh the Places You Will Go ... With a Penn State Degree” October 2018. Keynote Speaker. Pennsylvania State University Student STEM Open House. University Park, PA. 2. “What to Look for in a Graduate School” April 2015. Panel Speaker. Pennsylvania State University Student Support Services. University Park, PA.

3. “Graduate Student Experience at Penn State” February 2015. Panel Speaker. Pennsylvania State University Millennium Science Scholars. University Park, PA.

4. “How to Study for Science Courses” January 2015. Panel Speaker. Pennsylvania State University Student Support Services. University Park, PA.

5. “Letter of Recommendations for Graduate/Professional School Application” November 2014. Panel Speaker. Pennsylvania State University BGSA Education and Mentoring. University Park, PA. 6. Panel Speaker. September 2013. Pennsylvania State University Office of Graduate Educational Equity Programs. University Park, PA.

Discussed various topics to 1st year graduate students on graduate student life as a minority student at a Penn State University

Owusu CV, 4

TEACHING AND ADVISING

Science Guest Lecturer for Upward Bound 2014

Topic: Metabolism of Macronutrients and Micronutrients Graduate Schreyer’s Honor Student Advisor 2013-2015 Advised Pennsylvania State University undergraduate students from the Schreyer’s Honors College in completion of their undergraduate honors thesis. Students advised was trained to do basic scientific bench research, which included scientific writing, analyzing and interpreting data.

Graduate Teaching Assistant 2010-2011

The Pennsylvania State University, Department of Biology Courses: Introductory Physiology (BIOL 141) and Mammalian Physiology (BIOL 473) Student Mentor 2009

The Pennsylvania State University, Summer Experience in the Eberly College of Science Duties: Train and mentored 3 inner-city high-school students of Pennsylvania for summer research and end of the summer oral presentation competition, where my students won first-place. Academic Tutor 2006-2009

Southern Illinois University-Carbondale, Academic Initiatives Duties: Tutored 15 students in core curriculum math and sciences classes TECHNICAL SKILLS AND TRAININGS

Computer Skills: Expert in Excel, PowerPoint, Word, GraphPad, StataIC, Adobe Acrobat Pro, Publisher Research Electronic Data Capture (REDCap) and experience using Minitab Trainings: Institutional Review Board (IRB) Training, Biomedical Human Subjects Research (HSR), The Health Insurance Portability and Accountability Act (HIPAA) Training, Chemical Safety and Biosafety Training, Autoclave Safety Bloodborne Pathogen

SELECT AWARDS AND FUNDING

American Thoracic Society

Minority Trainee Development Scholarship 2018

Minority Trainee Development Scholarship 2017

The Pennsylvania State University

1st Place Award in Ag Springboard Competition 2015

Awarded $7,500 to start a non-profit organization geared towards implementing early exposure to science and research of secondary schools in West Africa

Supplement NIH Minority Grant 5R01HD066982-03 2013-2015 Alfred P. Sloan Scholarship 2012-2015

Bunton-Waller Fellowship 2012-2015

Southern Illinois University - Carbondale

3rd Place Award for Oral Presentation 2008

Ronald E. McNair Scholars Summer Research Institute Symposium 2008 Outreach Award – Ronald E. McNair Scholars Program 2008 Student Housing Resident Assistant 2007–2009

Ronald E. McNair Scholar 2006-2009

Illinois Louis Stokes Alliance for Minority Participation Scholar 2006-2009 Saluki Peer Mentor - Academic Initiatives 2006–2007 Owusu CV, 5

LEADERSHIP POSITIONS

Pennsylvania State University

President-Appointed Student Body Liaison 2014-2016 The Commission on Racial/Ethnic Diversity (CORED) Forum of Black Affairs Secretary - BGSA 2014-2015

Event Coordinator - Adventist Campus Ministries Event Coordinator 2013-2014 Education and Mentoring Committee Co-Chair – BGSA 2013-2014

Financial Literacy – Program Coordinator

Organized and executed tutor sessions for undergraduate students utilizing graduate students as tutors

Organized and executed State College elementary schools African- American Read-ins Student Mentor - State College High School Women African-American Support Group 2013 President - Adventist Campus Ministries President 2012-2013 Mentor - The Alcorn State:Pennsylvania State University Bridges to the Doctorate Program 2012-2015 PROFESSIONAL MEMBERSHIPS AND AFFILIATIONS

Weill Cornell Medical College of Cornell University American Thoracic Society 2016-2019

Pennsylvania State University

Black Graduate Student Association (BGSA) 2012-2015 American Physiology Society 2012-2015

American Society of Nutrition 2012-2015

Graduate Student Association 2011-2012

University of Alabama at Birmingham

Pittman Society for Clinical and Translational Science 2009-2010 PROFESSIONAL DEVELOPMENT

Graduate Student Representative - Middle States Re-accreditation 2015 21st Annual Compact for Faculty Diversity’s Institute on Teaching and Mentoring 2014 Moderator - SROP-McNair 2014 Research Symposiums 2014

Moderated oral presentations and question/answer sessions for Earth and Mineral Science Summer Scholars

20th Annual Compact for Faculty Diversity’s Institute on Teaching and Mentoring 2013 6th Annual Course on Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis. Little Rock, AK

COMMUNITY SERVICE

Activities Volunteer - Hearthside Nursing & Rehabilitation Center, State College, PA 2012-2015

Assisted residents with physical activities and games 80th Annual Pennsylvania Junior Academy of Science (PJAS) Competition 2014

Volunteer Judge: Judged oral presentations of students from grades 8 - 11 who conducted research in biology and microbiology for scholarship awards

Pennsylvania State University Graduate Exhibition 2014

Volunteer Judge: Critiqued poster presentations from a variety graduate school disciplines in art, music, engineering, psychology, chemistry, and life sciences for a monetary prize

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