Lashanda N Waller

ac7rjj@r.postjobfree.com

803-***-****

Summary

I have 18 years of experience, including roles as Technical Supervisor, Certifying Scientist, Pharmacology Researcher, and a Microbiology Graduate Specialist in multiple science arenas including Toxicology, Mass Spectrometry, Microbiology, Biochemistry, Molecular Biology, Pharmacology, and Proteomics.

Career Achievements

•18+ years in laboratory procedures/molecular techniques

•14+ years in mass spectrometry analysis

•7+ years in diabetes field/ pharmacological pathways

•6+ years in biochemical detection

•6+ years in microbiological characterization

•3.5+ years as analytical toxicologist in COLA and CAP accredited laboratory

Laboratory Procedures

•Quality Assurance/Quality Control

•Stock Verification

•Technical Support and Training

•Supervisory Duties

•Data Acquisition/Interpretative Software

•Workflow Review

•LIS/LIMS review and process

•Record Keeping (CLIA, COLA, and CAP required documentation)

Examples of Instrumentation Used and Data Reviewed

•ThermoFisher Scientific Ion Trap and Orbitrap

•Agilent Triple Quad Mass Spectrometer

•Agilent (HP) GC-MS/MS

•AB Sciex and Bruker MALDI-TOF-TOF

•LC Packings HPLC system

•Beckman Coulter Immunoanalyzer

•Tecan Automated Extraction System

Molecular Techniques

•Bacterial and mammalian cell culturing

•Electrophoresis (SDS-PAGE, isoelectric, OFFGel fractionation)

•Sample preparation (bacterial, blood, bone, cerebrospinal fluid, kidney, muscle, pancreas)

•Urine and oral fluid result interpretation

•Immunoassay preparation and result interpretation

•Protein extraction, immunoprecipitation, and overexpression

•Multiple staining techniques for cell, carbohydrate, and protein observation and detection

•PCR

Microbiology Training Procedures

Microbiological Techniques

•Cultured and purified class 2 agents (B. anthracis group)

•Expanded differential extraction methodologies for proteins, glycoproteins, and carbohydrates of B. anthracis and other members of the B. cereus group

•Biochemically derivatized sugar composition of macromolecules with alditol acetate procedure

•Utilized SDS-PAGE gradient gel electrophoresis and respective staining for protein and glycoprotein identification

•Purified and enzymatically digested proteins of interest

•Prepared bacterial cell strains for transmission electron microscopy studies

•Approved by Federal Bureau of Investigation for safety clearance of class 2 reagent work

Mass Spectrometry Techniques/ Instrumentation

•GC-MS/MS characterization of carbohydrate moieties of bacterial strains

•MALDI-TOF-TOF analysis for large size bacterial proteins

•Protein/peptide acquisition and data interpretation for scientific inquiry

Education

2006, Ph.D., Biomedical Science Program (Microbiology)

Department of Pathology, Microbiology, and Immunology

University of South Carolina School of Medicine, Columbia, SC

Project title: Discovery and Characterization of the Bacillus anthracis Glycoprotein BclB.

2000, B.S., Biology

Department of Biological Sciences

University of South Carolina, Columbia, SC

Computer/Software Skills

Agilent, AB Sciex, Bioworks, Chromeleon, LIS/LIMS, Mascot, MaxQuant, Microsoft Office, MultiQuant, Protein Pilot, ThermoScientific, Xcalibur

Technical Training Courses

Toxicology Laboratory Setup with AB Sciex Data Analysis (MultiQuant and Analyst), In-House Labs, Oklahoma, 2018

LC-MSMS 301: Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics, 2016

Agilent Technologies Customer Training Certificate (LC/QQQ and LC/Q-TOF certifier training), 2015

Clinical Research Training, Medical University of South Carolina, Charleston, SC, 2013

Mass Spectrometry of Glycoproteins, University of Georgia Complex Carbohydrate Research Center, 2010

Work Experience

Lab Technologist (Temporary Position)

Genova Diagnostics- Duluth, GA

July 2018 to September 2018

Process and analyze patient samples, monitor QC, ensure all instruments are maintained properly, and enter patient results in the department. Perform department procedures and demonstrate sound laboratory techniques. Demonstrates proficiency in judging acceptability of quality control results. Communicates sample issues within and outside the department to support proper client communication. Participates in internal and external proficiency testing. Analysis of 100-300 patient samples per day. Interpretation of immunoassay plates which includes standards and blanks for calibration purposes. Testing review includes Heliobacter pylori, Giardia lamblia, Cryptosporidium, pancreatic elastase-1, and eosinophil protein X.

Technical Supervisor, Certification Team

eLab Solutions - Atlanta, GA

January 2016 to May 2018

Supervisor over a team of 8 certification personnel for urine and oral fluid drug confirmation reporting. Provide liaison service in guidance, communication, and technical support across multiple departments in the laboratory. Responsible for answering calls of multiple clients in the area of toxicology and immunoassay screen results. Preparation of information for CAP accreditation process including review and entry of CAP proficiency specimens. Participant in supervisor and reporting group meetings for review of workflow and issues pertaining to work production. Other duties as outlined under Certifying Scientist position.

Certifying Scientist, Analytical Toxicology

eLab Solutions - Atlanta, GA

July 2014 to January 2016

Fulfill the requirements of testing personnel of a COLA/ CLIA/CAP Highly Complex Laboratory. Review LC-MS Calibration and Control data for acceptability (QC). Reviews EIA and LC/MS/MS data for various drugs of abuse in urine and oral fluids. Interpretation of LC/MS/MS data for various clinical applications. Review various chain of custody documents, certification and release the result to multiple clients including physicians. Provide EIA and LC/MS/MS maintenance and quality control. Provide chemistry specialty services in the absence of the laboratory director. Perform daily review of tests including over 70 drug analytes in association with pain management, illicit drugs, as well as specimen validity. Specialty testing review including EtG/EtS, GBP, MDPV, PPX, anti-psychotic drugs. Provide guidance and technical support to certification personnel. Primarily maintaining quantitative methods and standing orders maintenance. Participation in a weekend duty rotation and performance of peer reviews of certification team.

Pharmacology Researcher

Medical University of South Carolina - Charleston, SC

July 2010 to June 2013

Acted in a supervisory role to temporary and full time staff. Worked with multiple cell lines and conducting multiple molecular, biochemical, and analytical studies for multiple projects. The main project during this time was critical for changes seen in diabetic model systems. This included the measure of multiple post-translational modifications during the insulin/IGF-1 pathway. Sample log-ins, storage, and preparation were utilized for record keeping. Multiple molecular techniques incorporated for a wide variety of studies including electrophoresis, purification, and separation techniques. Analyzed samples with multiple mass spectrometry techniques including ion trap and Orbitrap. Use of instrument and corresponding data software for acquisition. Western blot analysis was utilized for densitometry studies.

Cardiovascular Scholar

Medical University of South Carolina - Charleston, SC

August 2006 to June 2010

During this employment, I was responsible for research and development of various projects in the facility. Also, I was responsible for training of some individuals in sample preparation and analysis. My involvement at this facility allowed interaction with coworkers within the laboratory, at the university, and outside of the university. Records were kept for all projects, which is the standard in this laboratory. These projects involved analytical and proteomic-based analysis of biological samples, particularly cerebrospinal fluid, sera, and muscle biopsy specimens.

Some tools included multiple column chromatography for analytical separation, dynamic range reduction, sample concentration, and peptide cleanup. Other duties were preparing samples for gel electrophoresis and isoelectric focusing for in-gel and off-gel formats (for protein and peptide isolates). Preparatory tools included liquid chromatography and strong cation exchange chromatography for peptide separation. Isobaric tag labeling was commonly used for quantitative measurement studies.

Mass spectrometry techniques included LC and MALDI-TOF-TOF mass spectrometry analysis. I used various instrument and data computer software for protein and peptide acquisition, as well as collective data interpretation. During the last period of this job, I was involved in a core facility, which involved analyzing samples for other individuals who were interested in using facility-provided services.

This job mainly included, but not limited to, quantitative analysis of various biological samples with sample preparation, data acquisition, data interpretation, and record keeping. Protocols were strictly adhered to, including proper storage, handling, and disposal of samples and waste for overall safety measures to prevent contamination and spread of potential biological threats throughout the laboratory. Each experiment was either a self or team-based effort, which has given me the advantage to be flexible in any work environment scenario.

Publications

Available upon request

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