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Maintenance Assistant

Location:
San Diego, California, United States
Posted:
November 02, 2018

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Resume:

Fnu Amrita

Email: ac7k7w@r.postjobfree.com

Landline: 801-***-****, Cell: 801-***-****

PROFESSIONAL SUMMARY

Having excellent research potential, knowledge and ability to actively contribute to the research projects goals. Can work in environment both independently and as part of a team.

Looking for an opportunity to work on an important research work/study which provides professional development, interesting experiences and personal growth.

AREA OF EXPERTISE

Computer skills: Proficiency in MS Word, Excel and Power Point. DNA-Star (Lasergene), Blast, prism etc. Expertise in using Filemaker and Lab-Guru, Online lab-website maintenance using wix.com

Management skills:

Maintaining appropriately laboratory safety procedures.

Maintain laboratory Equipment and inventory.

Keeping records of plasmids, strains, cell lines etc.

Placing orders and inventory lab supplies.

Managing order of compounds like salt, reagents, antibody, enzymes etc.

Cell Culture: Works with human cell lines i.e.HEK293, HEK293T, mouse cell lines i.e.min-6, RPE(Retinal Epithelial Cell), Cell line maintenance include media and solution preparation, harvesting, freezing and thawing of Cells, transfection and transduction. Microbial culture, Trypan Blue assay, Cell counting.

Molecular Biology: Design gRNA for CRISPR-Cas9(Gene Tagging and Inserting mutation), CRISPR Downregulation of gene (Adenovirus and Lentivirus backbone) construct), DNA & RNA extraction/quantitation, Nucleic acid & protein gel Electrophoresis, Western Blot, Standard and Real time PCR, Genetic manipulation, Restriction Digestion and Restriction Digestion free (RF) Cloning, Gateway cloning, Transformation. Sample preparation for sequencing, analysis sequencing sample.

Biochemistry: Protein purification using His column, GST-Pull-down assay, Co-Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Confocal Microscopy (LSM 510), Dual luciferase assay, BCA assay.

Histology: High pressure Freezing (HPM 100), Freezing Substitution (Leica AFS2) and Plastic Embedding of Nematode Caenorhabditis Elegans, TEM imaging (Hitachi 7100, Jeol 1400, FEI TF20). Expertise in making supporting film on TEM grids staining of TEM samples.

Animal Technique: Maintenance of mouse colonies and genotyping. Small mouse surgery, isolation of serum and plasma from mouse blood.Isolating eye tissue. Injection in C.elegans, Single Worm pcr, Sorting C.Elegans using COPAS machine on the basis of injection marker or any fluorescence gene, Brood analysis, Making integrated line of C.elegans by Gama-irradiation. Freezing and maintaining and Nematode Caenorhabditis Elegans strains, Pheromone Assay of C.elegans. Maintenance of different species of fish.

Chemistry: Spectrophotometer, Centrifugation, Quantitative transfer, Titration, Chromatography (Paper and plate) for separating organic compound, Akta-FPLC.

Career History:

The Scripps Research Institute, Department of Neuroscience, San Diego, California, USA

Research Assistant I June 2017-Present

Protein purification using His column, BCA assay

Design gRNA for Crispr-Cas9(Tagging gene and inserting mutation), Genetic manipulation, Restriction Digestion and Restriction Digestion free (RF) Cloning, Gateway cloning, Nucleic acid & protein gel Electrophoresis, Western Blot, Standard and Real time PCR. Micro-Injection in C.Elegans (Over-Expression of plasmid and endogenously)

Sorting C.Elegans using COPAS machine on the basis of injection marker or any fluorescence gene, Brood analysis, Making integrated line of C.Elegans by Gama-irradiation

Designing Primers for Genotyping using Prime3plus/NCBI.

Prepare and send samples for sequencing

Analyze the sequencing results

Freezing and maintaining Nematode Caenorhabditis elegans strains

Managing lab including placing orders, maintaining appropriately laboratory safety procedures, record keeping, and maintenance of laboratory Equipment. Maintaining the lab website using wix

Sanford Burnham Prebys Medical Discovery Institute, Department of Infectious and Inflammatory disease, San Diego, California, USA

Research Assistant II March 2017 -June 2017

DNA & RNA purification, Nucleic acid & protein gel Electrophoresis, Western Blott, Standard and Real time PCR, Genetic manipulation, Restriction Digestion and Restriction Digestion free (RF) Cloning, Gateway cloning, and transformation

Transfection, cell line maintenance (Freezing and Thawing of Cells, Microbial culture, Trypan Blue assay, Cell counting. Protein purification using His column, GST-Pull-down assay, Co-Immunoprecipitation, BCA assay).

Managing lab including placing orders, maintaining appropriately laboratory safety procedures, record keeping, and maintenance of laboratory Equipment.

Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA

Research Technician III September 2015 to March 2017

Working with Age related macular degeneration (AMD)

Generate several mouse models for AMD and using those models to elucidate pathophysiological mechanisms for AMD.

Maintain the transgenic mouse lines by breeding and genotyping, purification of RNA from eye tissue and perform real time PCR for quantification of several targets among these lines.

Make plasmids carrying dcas9 or sgRNAs together or alone and test sgRNA efficacy in the HEK293T or mouse MS1 cell line using, dual luciferase assay, immunocytochemistry, western blotting, real time PCR.

Screening sgRNA to target the inflammation in in vitro models. Make the plasmids using both restriction digestion and restriction free cloning and test their efficiency dual luciferase assay, immunocytochemistry, western blotting, real time PCR.

Lab management functions, including placing orders, maintaining appropriately laboratory safety procedures, record keeping, and maintenance of laboratory Equipment etc

University of Utah, Department of Biology, Salt Lake City, Utah, USA

Lab Technician January 2010 –August 2015

DNA purification, Nucleic acid & protein gel Electrophoresis, western blotting, Standard and PCR, Restriction Digestion and Restriction Digestion free (RF) Cloning, Gateway cloning, Transformation, transfection by Calcium- Phosphate/acidic PEI, Electroporation (Neon system), Protein purification using His column, GST-Pull-down assay. Cell line maintenance, Freezing and Thawing of Cells, Microbial culture, Trypan Blue assay, Cell counting etc

High pressure Freezing (HPM 100), Freezing Substitution (Leica AFS2) and Plastic embedding of nematode Caenorhabditis elegans, TEM imaging (Hitachi 7100, Jeol 1400, FEI TF20). Expertise in making supporting film on TEM grids staining of TEM samples.

Freezing and maintaining and nematode Caenorhabditis elegans strains, Pheromone Assay of C.elegans

Primary knowledge of Akta -Fplc (Unicorn Software).

Lab management functions, including placing orders, maintaining appropriately laboratory safety procedures, record keeping, and maintenance of laboratory Equipment etc.

Zebrafish core facility, University of Utah, Salt Lake City, Utah, USA

Lab Assistant September 2009-January 2010

Maintenance and feeding of different species of fish.

Moran Eye Center, University of Utah, Salt Lake City, Utah, USA

Volunteer March 2009-September 2009

DNA purification, Nucleic acid & protein gel Electrophoresis, western blotting, Standard and PCR, Restriction Digestion and Restriction Digestion free (RF) Cloning, Transformation, transfection by Calcium- Phosphate/acidic PEI, Electroporation (Neon system), Cell line maintenance, Freezing and Thawing of Cells, Microbial culture, Trypan Blue assay, Cell counting etc. Maintenance of mouse colonies and genotyping

Royal Institute of Technology, Department of Corrosion Science Stockholm, Sweden

Researcher September 2008 -January 2009

Spectrophotometer, Centrifugation, Quantitative transfer, Titration, Chromatography (Paper and plate) for separating organic compound.

Gurukul Kangri University, Department of Chemistry, Haridwar, India.

Research fellow June 2002 to August 2005

Spectrophotometer, Centrifugation, Quantitative transfer, Titration, Chromatography (Paper and plate) for separating organic compound.

R. K. Postgraduate College, Department of Chemistry, Shamli, India

Lecturer June 2001 to June 2002

Subject taught- Analytical Chemistry, Physical Chemistry, and Inorganic Chemistry

Education:

Master of Science, Chemistry (Organic chemistry) C.C.S.University Meerut, India, 1999-2001

Publications:

Kumar S, Nakashizuka H, Jones A, Lambert A, Zhao X, Shen M, Parker M, Wang S, Berriochoa Z, Fnu A, VanBeuge S, Chévez-Barrios P, Tso M, Rainier J, Fu Y Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy. 2Am J Pathol. 2017 Dec;187(12):2841-2857.

Zhang T, Cao LH, Kumar S, Enemchukwu NO, Zhang N, Lambert A, Zhao X, Jones A, Wang S, Dennis EM, Fnu A, Ham S, Rainier J, Yau KW, Fu Y. (2016). Dimerization of Visual Pigments in Vivo. Proc Natl Acad Sci USA. 113(32):9093-8.

Jones A, Kumar S, Zhang N, Tong Z, Yang JH, Watt C, Anderson J, Amrita, Fillerup H, McCloskey M, Luo L, Yang Z, Ambati B, Marc R, Oka C, Zhang K, Fu Y. (2011) Increased Expression of HTRA1 in Retinal Pigment Epithelium Induces Polypoidal Choroidal Vasculopathy in Mice. Proc Natl Acad Sci U S A. 2011 Aug 30; 108(35):14578-83.

Kaushik RD, Devi S, Shashi and Amrita. Periodate oxidation of 2,3-dimethyl aniline-Determination of Mn(II) in nanograms in aqueous medium. Indian J. Environ. & Ecoplan. 8(I):253-256, 2004

Kaushik RD, Singh RP, and Amrita. Kinetics & Mechanism of periodate oxidation of 2,3 dimethylaniline in acetone-water medium. J. Curr. Sci. 5(I), 07-12, 2004

Kaushik RD, Amrita, Singh RP and Devi S. Kinetic method for nanogram determination Mn(II) based on its catalytic effect on periodate oxidation of m-chloroaniline. J. Curr. Sci. 5(I), 341-344, 2004

Kaushik RD, Singh RP, Amrita. Periodate oxidation of p-bromoaniline in acetone–water medium: A kinetic mechanistic study. Asian Journal of Chemistry. Vol. 1, No. 2:831-836, 2004

Kaushik RD, Shashi, Amrita and Devi S. Kinetics and mechanism of Mn(II) catalyzed periodate oxidation of 4-chloro-2-methyl anilines. Asian Journal of Chemistry.Vol.16, No.2:818-822, 2004

Kaushik RD, Singh RP and Amrita. Kinetics & Mechanism of periodate oxidation of 2,3-dimethylaniline in acetone-water medium. J. Curr. Sci. 5(I), 07-12, 2004

Abstracts:

Sandeep Kumar, Stephanie VanBeuge, Zachary Berriochoa, Amrita Fnu, Yingbin Fu . Focal ERG analysis on the impact of PCV lesions on retinal function. Invest Ophthalmol Vis Sci 2016: E-Abstract 4780.

Kaushik RD, Amrita, Kumar K and Chaubey AK. Kinetics and mechanism of Mn(II) catalyst, periodate oxidation of o-chloroaniline, 92nd Indian Science Congress, January 2005, Nirma University, Ahmedabad, India

Kannaujiya S, Kumar K, Amrita, Devi S. Mn (II) catalyzed periodate oxidation of aniline in acetone- water medium-A kinetic mechanistic study, 92nd Indian Science Congress, January 2005, Nirma University, Ahmedabad, India



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