CURRICULUM VITAE

Personal data:

Family Name: RAD

First Name: MEHRNAZ

Birth Date: 1961

Genus: Female

Marital situation: Married, Two children

Address: ***- **** ****** *****, ***** Vancouver, V7P 3G1, BC, Canada

Cell phone: 604-***-****

Email: ac7i9t@r.postjobfree.com

Professional experience:

1986-1996: Researcher and then manager of the laboratory for preparation of culture media.

Department of Research and Production of Bacterial Vaccines and Colored Antigens, Pasteur Institute of Iran, Tehran, Iran

1996-2005: Lecturer and Diagnostic Bacteriologist

Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2005- 2013: Assistant professor and Manager of Diagnostic Bacteriology Laboratory,

Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2013- To date: Associate professor and Head of Diagnostic Bacteriology Laboratory,

Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Highlights of qualifications

•Skills in food and molecular microbiology, DNA extraction from bacteria, PCR method.

• Experience in genotyping methods for identification and detection of food pathogens.

• Experience in bacteriology: bacterial culture methods, preparing culture media, sterilization methods

• Skills in antimicrobial susceptibility tests: Disc diffusion method, MIC methods

• Skills in diagnostic methods for identifying and phenotypic classification of bacteria: biochemical tests and analyzing the results

•Experience in handling laboratory animals

•Experience in preserving methods for bacteria: Freeze-dry method, deep freeze, liquid nitrogen

•Skills in mammalian cell culture method: tissue culture preparation, maintaining cell lines

• Fluent in English and Persian

Education:

1976-1978: English language training courses of “Junior and Senior Proficiency”, Iran and America Cultural Association, Tehran, Iran.

1975-1979: High School, Diploma in Sciences, Azar High School, Tehran, Iran.

1979-1986: D.V.M., Doctor in Veterinary Medicine, Tehran University, Tehran, Iran.

2001-2005: PhD, in Veterinary Microbiology, Urmia University, Urmia, Iran.

2005 (6 month): Visiting Scientist, Department pf Pathology and Molecular Medicine, Center for Gene Therapeutics, McMaster University, Hamilton, Canada

Workshops:

1989: The course of “Methods of microorganisms preservation”- two weeks. By: Industrial and Scientific Researches Organization of Iran, Tehran, Iran.

1995: The course of “Genetic of bacteria”- one month.

Under supervision of Russian scientists of “Russian Academy of Medical Sciences” and “The Gamaleya Research Institute of Epidemiology and Microbiology”, in Pasteur Institute of Iran, Tehran, Iran.

2005: The workshop of "Rodent handling procedures workshops", in McMaster University, Faculty of Health Sciences held in July 2005.

2009: "Gene Cloning and Bacterial Transformation", in Faculty of Science, Ferdowsi University of Mashhad

Educational Experiences (giving lectures):

1. Undergraduate course: “Veterinary microbiology” for students of animal technician, school of Veterinary Medicine,

Ferdowsi University of Mashhad, Iran (1996-2002)

2. Undergraduate course: “Veterinary Virology” for students of animal technician, school of Veterinary Medicine, Ferdowsi University of Mashhad, Iran (1996-2002)

3. Undergraduate course: “Genenral bacteriology” for vetrinary medical students, school of Veterinary Medicine, Ferdowsi University of Mashhad, Iran (1996-2009)

4. Undergraduate course: “Specific bacteriology” for vetrinary medical students, school of Veterinary Medicine, Ferdowsi University of Mashhad, Iran (1996-2009)

5. Undergraduate course: “Advanced diagnostic techniques in veterinary microbiology ” for veterinary Laboratory sciences B.S. students, school of Veterinary Medicine, Ferdowsi University of Mashhad, Iran (2005-2009)

Membership in Scientific Organisations:

1-International Society for Infectious Diseases

2-Iranian Society for Microbiology

Publications:

1.Rad, M.; Seifi, H.A.; Movassaghi, A.R. (1998) Erysipelothrix septicaemia in neonatal lambs. J. Vet. Med. B45, 573-575

2. Seifi, H.A. ; Movassaghi, A.R. ; Mohri, M. ; Rad, M. ; Aslani, M.R. ; Parand, A. ; Razmi, Gh. (2000) Concurrent cocidiosis and bovine papular stomatitis infection in calves. J. Appl. Anim. Res.18, 103-108

3. Seifi, H.A.; Rad, M.; Madadi, M.S. (2001) Povidone iodine spray for treatment of infectious bovine keratoconjunctivitis. J. Fac. Vet. Med. Uni. Tehran 56, 100-103 (In Persian)

4- Shahsavani D, Rad, M (2001) The syndrome of hole in the head in severum (Cichlasomaherusseverus) on Oscar (Astronotusocellatus): a case report. J. Fac. Vet. Med. Uni. Tehran 56, 125-106 (In Persian)

5- Movassaghi AR, Rad M (2002) An outbreak of Yersinia pseudotuberculosis Infection in lambs. J. Appl. Anim. Res. 22, 125-128

6- Seifi, H.A.; Saifzadeh, S.; Farshid, A.A.; Rad, M.; Farrokhi, F. (2003) Mandibular pyogranulomatous osteomyelitis in a Sannen goat. J. Vet. Med. A50, 219-221

7- HashemiTabar GR, Rad M, Chavoshi M (2004) A survey on Dermatophilosis in sheep in the North of Iran. Iranian J. Vet. Res. 5(2): 97-101

8- Yousef-BeighiGh, Rad M, Hosseini SM, Jafari A (2005) Effect of centrifugation of milk samples on bacteriologic culture. Iranian J. Vet. Res. 6 (1): 59-61

9- Rad M., Ahmadi M., Farid Hosseini R., Ashkar A.A., Nejati M., Tabaraie B., Nourozian D. (2006) Protection against Listeria monocytogenes by ODN containing CpG motifs in BALB/c and C57BL/6 mice. J. Appl. Anim. Res., 145-147

10- Rad M., Ahmadi M., Farid Hosseini R., Ashkar A.A., TavakkolAfshari J. (2006) The role of interlukin12 in protection induced by CpG against Listeria monocytogenes in BALB/c and C57BL/6 mice. Comp. Clin. Pathol. 14:197-201

11- Janatabadi AA, Mohammadi GR, Rad M, Maleki M (2008) Molecular identification of strreptococcusequi subsp. Equi and streptococcus equi subsp.

Zooepidemicus in nasal swabs saples from horses suffering respiratory infections in Iran. Pakistan J. Biol. Sci. 11 (3), 468-471.

12- Rad M, Kalidari GH, Kurdjazi SH (2009) Isolation and identification of Salmonella spp. From Mazandran native poultry breeding and improvement center.PajhoheshVaSazandeghi (In Persian) 81: 87-93.

13-Rad M., Movassaghi A.R., Sharifi K., Naseri Z., Seifi H.A. (2009) Two outbreaks of pasteurellamultocida septicemia in neonatal lambs. Comp. Clin. Path.20: 57-59.

14- Khoshnegah, J.; KazemiMehrjerdi, H.; Rad, M. (2009)Successful treatment of a subcutaneous axillary abscess due to Staphylococcus lentus in a rabbit. Iranian Journal of Veterinary Science and Technology. 1(1), 41-46.

15- HashemiTabar,G.R.; Rad, M.; Naseri, Z.; Azizzadeh, M. (2009) Detection of antibody against infectious bovine rhinotracheitis glycoprotein gE in aborted cattle in Mashhad, Iran. Archives of Razi Institute. 64(2): 91-95.

16- Movassaghi, A. R.; Rad, M. (2010) A rare case of septicemia due to Bacteroidesmelaninogenicus in a Holestein cow. Comp. Clin. Path.19: 409-411.

17- Rad, M.; Shahsavani, D. (2010) Isolation and characterization of Vibrio (Listonella) anguillarum from catfish. Turk. J. Vet. Anim. Sci. 34(4): 413-415.

18- Tajik, J.; Mohammadi, G.R.; Rad, M.; Barati, A. (2010) Hemorrhagic bowel syndrome in dairy cattle in Iran: a case report. Iranian Journal of VeterinaryResearch. 11(2): 180-183.

19- AfshariSafavi, E.; Mohammadi, Gh. Naghibi, A.; Rad, M. (2011) Prevalence of Cryptosporidium spp. Infection in some dairy herds of Mashhad (Iran) and its association with diarrhea in newborn calves.Comp. Clin. Path.20: 103-107.

20- AfshariSafavi, E.A.; Mohammadi, G.R.; Rad, M.; Naghibi, A. (2012) A case- control study of association between diarrhea in newborn calves and infection with rotavirus and cornavirus in some industrial dairy herds of mashhad area, Iran in 2008. Archives of Razi Institute. 67(1): 35-41.

21- Rivandi, M.; Emami, M.R.; Rad, M.; KazemiMehrjerdi, H.; Azizzadeh, M.; Ghasemi, S. (2012) Bacteriological evaluation of Aleovera L. fresh gel on experimental infected full-thickness open wounds induced with staphylococcus aureus in dogs. Iranian Journal of Veterinary Surgery, 7(1,2): 75-83.

22- Rad, M.; Kooshan, M.; Mesgarani, H. (2012) Quinolone resistance among Salmonella entericaand Escherichia coli of animal origin. CompClinPathol, 21:161–165.

23- Khoshnegah, J.; Movassaghi, A.R.; Rad, M. (2013)Survey of dermatological conditions in a population of domestic dogs in Mashhad, Northeast of Iran (2007-2011). Veterinary Research Forum. 2013; 4 (2): 99-103.

24- Shahrokhi, V.; Rad, M.; Kalidary, A. (2013) Treatment of newly hatched chicken with CpGoligodeoxynucleotides decreases liver/spleen colonization of salmonella entritidis in broiler chickens. Comp. Clin. Pathol. 22:935-939.

25- HashemiTabar, G.R.; Rad, M.; Naseri, Z. (2014) Serodiagnosis of Brucella infection in aborted cattle in dairy herds in Mashhad, northeast of Iran. Journal of Zoonozes, 1(1): 42-48.

26- Kalidari, Gh.A; Razmyar, J.; Rad, M.; Bibak, F. (2013) Isolation of Mycoplasma spp. From broiler flocks with respiratory syndrome in Mashhad, Iran.Iranian Journal of Veterinary Science and Technology. 5(1): 11-18.

27- FirozMian,M.; Ahmed, A.N.; Rad, M.; Babaian, A.; Bowdish, D.; and Ashkar, A. (2013) Length of dsRNA (polyI:C) drives distinct innate immune responses depending on the cell type. Journal of Leukocyte Biology. 94: 1025-1036.

28- Halimi, H.A.; Seifi, H.A.; Rad, M. (2014) Bovine salmonellosis in northeast of Iran: frequency, genetic fingerprinting and antimicrobial resistance patterns of salmonella spp. Asian Pacific Journal of Tropical Biomedicine. 4(1): 1-7.

29- Razmyar, J.; Kalidari, G.A.; Tolooe, A.; Rad, M.;Movassaghi, A.R. (2014) Genotyping of Clostridium perfringens isolated from healthyand diseased ostriches (Struthiocamelus). Iranian Journal of Microbiology, 6: 31-36.

30- RezaeiGolestani, M.R.; Rad, M.; Bassami M.R.;Afkhami, A. (2015) Analysis and evaluation of antibacterial effects of new herbal formulas, AP-001 and AP-002, against Escherichia coli O157:H7. Life Science, 135:22-26

31-Afshari, A.; Jamshidi, A.;Razmyar, J.; Rad, M. (2015) Molecular typing of Clostridium perfringens isolated fromminced meat.Iranian Journal of Veterinary Science and Technology, 7: 32-39.

32- Afshari, A.; Jamshidi, A.;Razmyar, J.; Rad, M. (2015)Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran. Veterinary Research Forum, 6 (4) 279 – 284.

33- Jamshidi, A.; Rad, M.; Zeinali, T. (2015)Detection of Shiga toxin-producing Escherichia coli(STEC) in faeces of healthy calves in Mashhad, Iran. Archives of Razi Institute, 70(3): 179-185

34- Gholamhosseini-Moghaddam1, T.; Rad,M.; Mousavi, S.F.; Ghazvini, K. (2015) Detection of lytA, pspC, and rrgA genes in Streptococcus pneumoniae isolated from healthy children. Iranian Journal of Microbiology, 7 (3 ): 156-160.

35- Zeinali, T.; Jamshidi, A.; Rad, M.; Bassami,M.A. (2015) A comparison analysis of Listeria monocytogenesisolates recovered from chicken carcassesand human by using RAPD PCR. Int J ClinExp Med, 8(6):101**-*****.

36- Zeinali, T.; Jamshidi, A.; Bassami, M.A.; Rad, M. (2015)Serogroup identification and Virulence gene characterization ofListeria monocytogenes isolated from chicken carcasses. Iranian Journal of Veterinary Science and Technology,7(2): 9-19.

37- Rad, M.;Kooshan, M. (2015) A study on the presence of some potential virulence genes andquinolone resistance in avian pathogenic Escherichia coli isolatedfrom chickens in Northeast of Iran. Iranian Journal of Veterinary Medicine, 9(4): 271-278.

38- Abiri, Z;Khalili,M; Rad, M.; Sharifi, H. (2016) Detection of Coxiellaburnetii in Aborted Fetuses of Cattle and Sheep Using Polymerase Chain Reaction Assay in Mashhad City, Iran. Int J Enteric Pathog.4(1): e33170.

39- Afshari, A.; Rad, M.;Seifi, H.A.;Ghazvini, K. (2016) Genetic variation among Escherichia coli isolates from humanand calves by using RAPD PCR. Iranian Journal of Veterinary Medicine, 10: 33-40.

40-Barzan, M.; Rad, M.;HashemiTabar, G.R. (2017) Phylogentic analysis of Escherichia Coli isolates from healthy and diarrheic calves in Mashhad, Iran. Bulgarian Journal of Veterinary Medicine, 20(1): 11-18.

41- Zeinali, T., Jamshidi, A., Bassami, M. and Rad, M.(2017) Isolation and identification of Listeria spp. in chicken carcasses marketed in

northeast of Iran. International Food Research Journal, 24(2): 881-887.

42- Zeinali, T., Jamshidi, A., Rad, M., and Bassami, M. (2017) Analysis of Antibiotic Susceptibility Profile and RAPD Typing of Listeria Monocytogenes Isolates. J Health Sci Technol, April;1(1):11-16.

43- - Zeinali, T., Jamshidi, A., Ghazvini, K., Bassami, M., and Rad, M. (2017) Prevalence of Listeria monocytogenes in cerebrospinal fluid obtained from hospitalized patients. Comparative Clinical Pathology (In press).

44- Bahraminia, F., Emadi, S.R., Emaneini, M., Farzaneh, N., Rad, M., Khoramian, B.(2017) A high prevalence of tylosin resistance among Staphylococcus aureus strain isolated from bovine mastitis. Veterinary Research Forum: 8 (2) 121 – 125.

45- Dabiri, M., Mirshokraei,P., Rad, M., Khoramian, B. (2017) Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran. IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY:9:2, 28-32.

46- Ebrahimi-Nik, H., Bassami, M., Mohri, M., Rad, M., Khan,M. (2018) Bacterial ghost of avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian colibacillosis. PLOS ONE https://doi.org/10.1371/journal.pone.0194888 March 22, 2018.

Research Experiences:

1985-1986: D.V.M. Thesis - “Identification of Theileriaannulata by indirect immunofluorescent antibody method”, Faculty of Veterinary Medicine, Tehran University and Pasteur Institute of Iran.

1985-1986: AS a part of D.V.M. Thesis - “Production of bovine conjugated antiserum for indirect immunofluorescent antibody test”, Department of Biochemistry, Pasteur Institute of Iran and Department of Parasitology, Faculty of Veterinary Medicine, Tehran University.

1987-1988: Junior Researcher - “Isolation of Brucella abortus in ticks by indirect immunofluorescent antibody test”, Department of Parasitology, Pasteur Institute of Iran.

1989-1991: Research Associate - “Production of Salmonella anti-serums”, Department of Research and Production of Bacterial Vaccines and Colored Antigens, Pasteur Institute of Iran.

1997-1999: Senior Researcher - “Culture collection of bacteria”, School of Veterinary Medicine, Ferdowsi University of Mashhad.

1998-1999: Senior Researcher - “Preservation of Neisseria flavasub.spp. subflava by three methods”, School of Veterinary Medicine, Ferdowsi University of Mashhad.

1999-2000: Senior Researcher - “ Identification of Enterobacteriaceae involved in yolk sac infection in chicken”, School of Veterinary Medicine, Ferdowsi University of Mashhad.

1999-2000: Senior Researcher - “Identification of aerobic and anaerobic gram positive bacteria involved in yolk sac infection in chicken”, School of Veterinary Medicine, Ferdowsi University of Mashhad.

2000-2001: Senior Researcher - “Identification of Escherichia coli strains by the use of biotyping and serotyping methods”, School of Veterinary Medicine, Ferdowsi University of Mashhad.

Presentation in Congresses:

*10th International Conference on Production Diseases in Farm Animals, 24-28 Aug. 1998, Utrecht - The Netherlands

Rad, M.: Invitro antimicrobial susceptibility test on bacterial agents isolated from clinical mastitis.

*11th Iranian Veterinary Congress, 7-9 March 1999, Tehran - Iran

Rad, M.; Keleidari, Gh.;Golpari, R. : Invitroantimicobial susceptibility of Escherchia coli isolated from CRD complex in chicken.

*5th International Sheep Veterinary Congress, Jan. 2001, Stellenbosch - South Africa

HashemiTabar, G.R.; Rad, M. : The first report of Dermatophilosis in sheep in Iran.

*The 1st Iranian Congress on Applied Biology, Feb. 2001, Mashhad - Iran

Rad,M.; Miameie,A. : Study on effects of glycerol on bacterial viability at temperatures below and above 0 C.

*The 1st Iranian Congress on Applied Biology, Feb. 2001, Mashhad - Iran

GhodratiAzadi,H.; Rad,M.; Jafari,J. : The antibacterial effects of Nigelia. Sativa.

*11th International Conference on Production Diseases in Farm Animals, 12-16 Aug. 2001, Copenhagen - Denmark

Rad, M.; Esmailnejad, Z.; Keleidari, Gh. : Identification of gram positive bacteria involved in yolk sac infection.

*11th International Conference on Production Diseases in Farm Animals, 12-16 Aug. 2001, Copenhagen - Denmark

Farzaneh, N.; Rad, M.; Saadati, J. : Evaluation of Levamisole administration at dry period for controlling postpartum bovine mastitis.

*19th Meeting of the European Society of Veterinary Pathology, 25-28 Sep. 2001, Thessaloniki - Greece

Movassaghi, A.R.; Rad, M. : An outbreak of Yersinia pseudotuberculosis infection in lambs.

*The first National Iranian Conference of Applied Microbiology, July, 2007, Tehran, Iran.

Rad, M.; GholamhosseiniMoghadam, T: Phenotypic characterization of avian Escherichia coli by antibiogram testing.

*13th International Conference on Production Diseases in Farm Animals, 29 July-4 August 2007, Leipzig, Germany

Rad, M.; Yousef-Beigi, G.H.; Hosseini, M. : Effect of preculture freezing and incubation on recovery of staphylococci from subclinical bovine mastitis

*13th International Congress on Infectious Diseases, 19-22 June 2008, Kuala Lumpur, Malaysia

Rad, M.; Naseri, Z.; Bacterial causes of ovine abortion and neonatal mortality in Iran.

*26th Annual Meeting of the European Society of Veterinary Pathology, 17-21 september 2008, Dubrovnik, Croatia

Movasseghi, A. R.; Rad, M.: Arare case of septicemia due to Bacteroidesmelaninogenicus in Holstein cow

*The second Iranian Congress of Clinical Microbiology, 8-10 october, 2008, Shiraz, Iran.

Rad, M.; Mesgarani, H. : Quinolone resistance among animal strains of Salmonella and Escherichia coli

*1st International Congress on Aquatic Animal Health Management and Diseases, 27-28 Jan., 2009, Tehran, Iran

Mousavi, S.; Shahsavani, D.; Rad, M. : Casualties arising from Plesiomonasshigelloides in rainbow trout in Sabzevar.

*1st International Congress on Aquatic Animal Health Management and Diseases, 27-28 Jan., 2009, Tehran, Iran

GholipourKanani, H.; Shahsavani, D.; Rad, M. : Streptococcal septicemia in rainbow trout farm in Sabzevar.

*The 3rd Eurasia Congress of Infectious Diseases, 1-4 Oct., 2009, Baku, Azerbaijan

Rad, M.; Kooshan, M. : Virulence genes of Escherichia coli isolates from poultry with colibacillosis.

Book:

Veterinary Microbiology: Bacterial and fungal agents of animal disease.

2005 By: J. Glenn Songer and Karen W. Post

Translated in Persian by:

Gholamreza Hashemi Tabar and Mehrnaz Rad

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