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Biologics Process Development Engineer

Location:
Cambridge, Massachusetts, United States
Salary:
75000
Posted:
October 15, 2018

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Resume:

Vinit Joshi

ac7d5e@r.postjobfree.com

617-***-****

SUMMARY

Protein biochemist with experience in downstream process development and protein purification at lab scale and pilot scale. Good analytical and problem-solving skills. Strong work ethic and eagerness to learn and explore different aspects of downstream development and beyond.

EXPERIENCE

MOMENTA PHARMACEUTICALS INC, Cambridge, MA

Associate Process Engineer September 2017 – Present

Experience with downstream process development of Biosimilar and Novel antibodies.

Worked on and successfully designed a purification process for 2 biosimilar products which are currently in late stage Pre Clinical development or clinical trials.

Designed and implemented scalable downstream purification processes involving various filtration and chromatography steps to improve efficiency and reduce costs.

Experience in harvest clarification, concentration by Tangential Flow Filtration (Pendotech) and other depth filtration steps.

Purified large molecules using various chromatography steps on AKTA Avant, column packing on a lab scale.

Wrote and edited methods to perform various chromatography and screening experiments on Unicorn software for AKTA systems.

Designed and optimized Ultrafiltration/Diafilteration experiments with bench scale and Pendotech systems.

Familiar with designing multivariate experiments for testing filters, dynamic binding capacity studies, Isoform modulation of antibodies.

Worked on Tech transfer activities which involved writing Process description, batch records and process summary documents.

Familiar with working in the Pilot scale processing lab (Up to 250L) and worked on AKTA Pilot, larger scale filtration systems and packing columns.

MOMENTA PHARMACEUTICALS INC, Cambridge, MA

Biologics Process Development Co-Op January 2017-June 2017

Worked in the downstream process development team on purifying large scale protein batches using Pro-A and ion-exchange chromatography on the AKTA Avant, AKTA Pure system.

Purified the protein harvest batches using Single pass TFF, XOHC/DOHC filters, chromatography steps and Ultrafiltration/ Diafiltration techniques.

Worked on sucrose offset optimization studies for a drug compound using UF/DF system.

Packed columns, prepared buffers and presented data in weekly lab meetings.

CENTER FOR DRUG DISCOVERY, NORTHEASTERN UNIVERSITY, Boston, MA

Graduate Research Assistant May 2016-December 2016

Determining the biochemical structure of the human MGL enzyme and the effect of mutations on its activity.

Harvested bacterial cells with different mutations, carried out protein purification by Affinity chromatography.

Characterized protein and its activity by techniques like NMR Spectrometry, ELISA, HPLC, SDS-PAGE.

Worked on isolating and studying metabolites of small molecule compounds using LC-MS system and determining their activity through binding assays.

EDUCATION

Northeastern University, Boston, MA May 2017

Masters in Pharmaceutical Sciences GPA: 3.6

Mumbai University, India. May 2015

Bachelors in Pharmaceutical Sciences GPA: 3.5

TECHNICAL SKILLS

Techniques: ELISA, Western Blotting, Affinity Chromatography, Process development, SDS-PAGE, Cell culture, Clonogenic Assay, Nanoparticle synthesis and conjugation, Protein purification, UF/DF, Single Pass TFF, HPLC, Avant AKTA.

Software: Unicorn 6.4, Unicorn 5.1, JMP, Empower Pro, Agilent, Analyst, GraphPad, Pandotech, Chemdraw, ImageJ, Spotfire, Microsoft office.



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