Research Biotechnology Cell Biology and Molecular Biology.

Curriculum Vitae

Name: Mohammad Tariq Ali Khan

Contacts: Phone +91-727*******; E-mail ************.*********@*****.*** Present position:

****-**** ******* ****** ******** Fellow in DBT funded GAP0224 project in the laboratory of Reproductive Health Sciences under supervision of Dr. Rajesh Kumar JHA, Scientist, Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India (1 year).

Title "Evaluation of TGF-β1 mediated signaling mechanism in endometriosis using mouse model"

Characterisation and discovery of novel molecules in disease like cancer, endometriosis and Poly Cistronic Ovarian Syndrome (PCOS).

Cellular events like MTT, Wound healing, Trans-well chamber invasion, Spheroid (3D), Clonogenic assay and 3D cell culture in cell biology.

Molecular tools and techniques like DNA, RNA and Protein isolation run Western blot and PCR in molecular biology.

In vitro (Cell lines- cell culture) and in vivo (Rodent-mouse& rat handling) study.

Screening of newly synthesized drugs using drug biology.

Screening of new contraceptive drug synthesized at CSIR-CDRI, Lucknow.

Embryo, Infertility, pregnancy in females for implantation.

Cancer biology, Epigenetics and Reproductive biology. Academic/ Professional Qualifications:

2014-2016

Master of Technology (Biotechnology, First class), Institute of Engineering

& Technology, Lucknow, India.

Thesis “Epigenetic modulatory phytochemicals sensitizes the effect of centchroman against human breast cancer MDA-MB-231 cells” in laboratory of Cancer Epigenetics at under supervision of Dr Syed Musthapa Meeran, Senior Scientist, CSIR-Central Drug Research Institute, Lucknow, India.

2008-2012

2008

2006

Bachelor of Technology (Biotechnology, First class), Amity University, Lucknow, India.

Class XII (Biology, equivalent to Distinction), CBSE, HAL School, India. Class X (Science, equivalent to Distinction), CBSE, HAL School, India. Awards:

2018

2017-2018

2014-2016

International English Language Testing System (IELTS) with overall 6.5 band, CEFR level: B2, Academic module, British Council, United Kingdom. Project Junior Research Fellow, 360000 INR, Department of Biotechnology, Ministry of Science and Technology, Government of India. Graduate Aptitude Test in Engineering (GATE Fellow), All India Rank-754, 300000 INR, MTech, AICTE, MHRD, Government of India. Membership of Professional Bodies:

2017-present

2015-2016

Junior member (ID: 273), Cancer Epigenetics Society, Vienna, Austria. Training & Placement Coordinator (Technical group), IET, Lucknow, India. Professional Trainings:

2012 Production & quality management of all carbonated and non-carbonated drinks

(3 months), Summer training, Coca Cola, Barabanki, India. 2011 DNA Forensics (45 days), Summer training, BioAxis DNA Research Centre, Hyderabad, India.

2010 Basic Tools & Techniques in Molecular Biology (30 days), Summer training, Biotech Park, Lucknow, India.

Technical Skills:

Cell Biology Cell culture, MTT assay, wound healing assay, matrigel invasion assay, annexin V apoptosis assay, cell cycle analysis using PI staining, 3D spheroids assay and clonogenic assay.

Molecular

Biology

Isolation of DNA, RNA & protein, plasmid DNA isolation from E. coli, genomic DNA isolation, quantification using Nano drop & qualitative analysis of DNA using agarose gel, SDS-PAGE, PCR, RFLP and RAPD, transfection using siRNA & miRNA and Western blotting.

Microbiology E. coli culture, antibiotic sensitivity test. Bioinformatics Aligning tools, primer designing, Rasmol, Swiss PDB viewer and several other Expasy tools, protein docking and Ramachandran plot. Animal

handling

Established models using mouse for endometriosis and breast cancer using swiss & C57Bl-6, PCOS using SD-rats, DBA/2 and CBA/J miscarriage abortion models, dosing, treatment and tissue collection. Biostatic Data analysis using GraphPad PRISM, a commercial scientific 2D graphing and statistics software published by GraphPad software, ImageJ software and TotalLab Quant software.

Publications in international journals:

Soni UK, Chadchan SB, Kumar V, Ubba V, Khan MTA, Vinodh BSV, Konwar R, Bora HK, Rath SK, Sharma S, Jha RK. A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization and invasiveness. Biology of Reproduction (2018) [in press]. Conference presentation:

Soni UK, Khan MTA, Jha RK. Integrin Linked Kinase is a key regulator of sequential cellular events that require during MCP-1-induced endometriosis. International Congress of Cell Biology-2018, CSIR-The Centre for Cellular & Molecular Biology, Hyderabad (2018). Poster presentation.

References:

Dr Syed Musthapa Meeran Dr Bhartendu Nath Mishra Dr Mohammad Israil Ansari Senior Scientist, Head of Department, Associate Professor, CSIR-Central Food

Technological Research,

Institute, Mysore, India.

Institute of Engineering &

Engineering

Lucknow, India.

University of Lucknow,

Lucknow,

India.

Email: *.********@*****.***.** Email: ************@*****.*** Email: *********@*******.**.** Phone- +91-840******* Phone-+91-879******* Phone- +91-983*******

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