Engineering Assistant

JOSHUA SHEN-HUAN KHOO

** **** **** **** ********, NY 10562 +1-914-***-**** *************@*****.***

SUMMARY

Biomedical engineering college graduate with laboratory experience seeking an entry level research or equivalent technical position.

EDUCATION

Bachelor of Engineering

The City College of New York, New York, NY

1 June 2018

Major – Biomedical Engineering

Minor – Physics (Biomedical concentration)

SKILLS & ABILITIES

Recombinant protein expression

Bacteria transformation, bacteria culture, mini-prep, midi-preps, induction of protein expression, nickel ion column purification, TEV protease cleavage, dialysis, lyophilization

Protein purification

Reverse phase column purification

High performance liquid chromatography with UV-VIS detector

Protein characterization

SDS-PAGE

Heme (reduced and oxidized states) binding with de novo designed protein using UV-VIS spectrometer

Circular dichroism of de novo designed protein (manganese binding protein)

Cell culture and cell based assays

Culture and co-culture of cancer cell lines with immune cells, cell counting, trypan blue staining, light microscopy

Bioluminescent and fluorescent based assays, fluorescence microscopy

DNA technologies

Restriction enzyme digestion, ligation and molecular cloning, agarose gel electrophoresis

Polymerase chain reaction

Mechanical testing of materials (biological and non-biological)

Stress, strain, creep, stress relaxation

Computational skills and data analysis

Python Programming

MATLAB

GraFit Data analysis

TopSpin NMR Data analysis

BLAST Basic Local Alignment Search Tool

EXPERIENCE

Research Assistant (Part-time/volunteer)

City College of New York, New York

2014-2017

Area of research – Synthetic biology (protein engineering) for therapeutics (drug activator) and other purposes

Expression of a de novo designed protein as a drug activator

Expression of de novo designed protein with improved heme binding

Column, HPLC purification and dialysis

SDS PAGE and UV-VIS Spectrophotometry

Circular dicryoism of manganese binding de novo designed protein

Research Trainee

Institute for Medical Research, Kuala Lumpur, Malaysia

Jul-Aug 2017

Area of research – cancer research

Co-culture of cancer and immune cells, viability assays

Optimization of low cost real time polymerase chain reaction equipment

Bioinformatics – sequence alignment, prediction of drug targets

Referees:

Dr. Ronald Koder, Department of Physics, Division of Science, CDI 1.308, City College of New York, 160 Convent Ave, New York, NY. E-mail: ******@****.****.***

Dr. Mitchell B. Schaffler, Department of Biomedical Engineering, Grove School of Engineering, ST-564, City College of New York, 160 Convent Ave, New York, NY. E-mail: **********@****.****.***

Dr. Susannah P. Fritton, Department of Biomedical Engineering, Grove School of Engineering, ST-403E, City College of New York, 160 Convent Ave, New York, NY. E-mail: *******@****.****.***

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