Assistant University

Nimish Kathale

*** ********* **, *** ***, Santa Clara, CA - 95054 n ************@*****.*** 650-***-**** https://www.linkedin.com/in/nimish-kathale/

Highly motivated and innovative molecular biologist with specialization in circadian biology, molecular genetics, cell biology, microbiology, and biochemistry. Expertise in development and execution of genetic and biochemistry-based research methods. Passionate about learning new scientific skills. Enthusiastic team player adept at providing leadership while also learning from fellow team members. Experienced in managing multiple projects simultaneously. Effective communicator and teacher that can conceptualize complex biological concepts to audiences with varied expertise. Area of expertise include:

Mammalian circadian biology Mammalian Cell Culture CRISPR & RNAi technology

Protein interaction studies High throughput screening Molecular cloning & Viral transfection Education

The University of Memphis, USA

Doctor of Philosophy in Biology May 2018

Thesis: “Biochemical Characterisation of the Oscillatory Function of CLOCK” The University of Memphis, USA

Master of Science in Biology May 2015

Bangalore University, India

Master of Science in Biotechnology June 2008

University of Pune, India

Bachelor of Science in Biotechnology May 2006

Experience

Research projects

The University of Memphis, USA 08/2010 - Current

Title: The Biochemical Characterization of the Oscillatory Function of CLOCK.

● The systematic analysis of the CLOCK protein to determine the motif/domain responsible for its oscillatory function using genetic and biochemical approaches.

● Identified two responsible motifs that confer oscillatory function onto CLOCK. Title: Screening of LOPAC for circadian phenotypes.

● The systematic high throughput screening of LOPAC (Library of Pharmaceutically Active Compounds) in circadian reporter cell-lines to identify novel circadian modulators and regulators.

● Identified ~20 novel circadian regulators and modulators. Graduate Teaching Assistant

The University of Memphis, USA 08/2010 - 12/2017

● Teach microbiology lab techniques of handling, culturing, staining, differentiating and characterizing bacteria in a completely hands-on approach for biology majors and non-majors.

● Tutor for Genetics, Microbiology, Molecular Biology, Biochemistry and Biological Clocks.

● Introductory lectures on mammalian circadian clock mechanism to graduate students. Graduate Teaching Assistant

University of Buffalo, USA 08/2009 - 05/2010

Course: Evolution and Cell Biology.

● Covered the concept evolution using creative methods for the students to understand the basic concepts of evolution.

● Tutored students in Cell Biology techniques in a complete hands-on experience. Research project

University of Pune, India 0 7/2005 - 05/2006

Title: To test the antibacterial activity of plant extracts of E ucalyptus globulus and F icus glomerata.

● The evaluation of leaf extracts of E ucalyptus globulus and fruit extracts of F icus glomerata as antibacterial agents against common pathogenic bacteria.

Summer Intern

MITCON Consultancy & Engineering Services Ltd, India 05/2004 - 07/2004

● Involved in initiating and establishing Plant Cell Cultures for several strains of maize and corn.

● Had an important role to play in standardizing bacterial Large Scale Manufacturing Protocols for several bacteria involved in commercial preparations.

Skills & Techniques

Molecular Biology

● DNA isolation (plasmid and genomic), RNA isolation, Primer design and gene cloning, site-directed mutagenesis and chimera generation, Gateway® cloning, PCR, qPCR, DNA sequence analysis, Western blotting, Co-immunoprecipitation (Co-IP), bacterial reporter assays, Transient cell-based reporter assays, Viral generation and transfections, Real-time cellular rhythm recording, SDS-PAGE. Genetics

● CRISPR, RNAi (shRNA and siRNA) knockdown studies, High throughput genetic assays, Forward and Reverse genetic assays, Genomics.

Cell Culture

● Cell line maintenance (HEK293T, MMHD-3, 3T3, 3T3-L1), Lenti-viral transfection (transient and stable), Plasmid transfection, RNAi (shRNA and siRNA knockdown studies), Single cell cloning and selection, Development of reporter cell-lines.

Publications

● Prevalence of cycling genes and drug targets calls for prospective chronotherapeutics; N imish D. Kathale, Andrew Liu; Proc. Natl. Acad. Sci. U.S.A; 2014.

● Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters; Chidambaram Ramanathan, Sanjoy K. Khan, N imish D. Kathale, Haiyan Xu, and Andrew C. Liu; Journal of Visualised Experiments; 2012.

● mTOR Signaling Regulates Central and Peripheral Circadian Clock Function; Chidambaram Ramanathan, Nimish D. Kathale, D ong Liu, Choogon Lee, David A. Freeman, John B. Hogenesch, Ruifeng Cao, Andrew Liu; PLOS Genetics; 2018.

Presentations & Talks

● Comparison of chemosensation in C. elegans between mutants with complete lack of GRK-2 function versus a point mutation in the PH domain of GRK-2. (Poster presentation at SUNY Buffalo; March 2010)

● Biochemical Characterisation of the Oscillatory Function of CLOCK (Poster presentation at University of Memphis; March 2015)

● Molecular, Cellular and Physiological Mechanisms of the Mammalian Circadian Clock (Poster presentation at NCUR-University of Memphis; April 2017)

● Biochemical Characterisation of the Oscillatory Function of CLOCK (Talk at RISER, Middle Tennessee State University, May 2017)

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