Associate Scientist
Resume:
Education
Quest University Canada
Squamish, BC
Senior thesis: Where we are: understanding the relationship between the entorhinal cortex and the hippocampus through remapping events in a novel behavioral task.
2009-2013
Compass Montessori Secondary
Golden, CO
Research
2017-
Astellas Research Institute of America
La Jolla, CA
Associate Scientist
I am a support member for a number of projects investigating potential causes and treatment of central nervous system disorders. I also develop protocols for new techniques or methodologies that have the potential to be used in the future. Specific skills: Perfusion-assisted clearing of tissue, one-photon calcium imaging, stereotactic surgery, ELISA, western blotting, qPCR, cell culture and transfection, acute slice recording, and immunocytochemistry.
2017-2018
Daneman Lab; Nudleman Lab
University of California, San Diego, CA
Lab Assistant
I was responsible for the care and maintenance of a mouse colony and designed my own experiment characterizing the retinal vasculature structure in mouse models of oxygen-induced retinopathy. Specific skills: Colony maintenance, tissue preparation, mouse handling and injections, perfusion, and genotyping.
2016-2017
Robert Sutherland Lab
Canadian Center for Behavioral Neuroscience, Lethbridge, AB
Research Assistant
I implemented my own experiment, which investigated immediate early gene activity in the entorhinal cortex as influenced by a novel behavioral task. Specific skills: Confocal and conventional stereology, advanced microscopy, immunohistochemistry and in situ hybridization, as well as advanced animal handling techniques such as aseptic technique, basic surgery, perfusion, and behavioral tasks.
2015
Kim Prisk Lab
University of California, San Diego, CA
Undergraduate Research Fellow
I was responsible for leading a study of my own design on characterizing the lung density of endurance athletes using fMRI imaging. Specific skills: MRI data analysis, MATLAB programming, SNR analysis, physiology tests, and knowledge of experimental design.
Publications, Posters, and Presentations
2018
Hippocampus
DOI: 10.1002/hipo.22843
Accepted Article [In production]
Lee JQ, LeDuke DO, Chua K, McDonald RJ, Sutherland RJ. Relocating cued goals induces population remapping in CA1 related to memory performance in a two-platform water task in rats.
2017
Canadian Association for Neuroscience Meeting
Montreal, QC
Peer-Reviewed Abstract
Authors: Lee JQ, LeDuke DO, McDonald RJ, Sutherland RJ. Cue-place memory representation and interaction in CA1 during spatial navigation and reorientation in rats.
Canadian Association for Neuroscience Meeting
Montreal, QC
Peer-Reviewed Abstract
Authors: LeDuke DO, Lee JQ, Sutherland RJ. Population remapping in the entorhinal cortex and its role in mediating navigation in a novel water task in rats.
2016
Squamish Public Library
Squamish, BC
Invited Talk
Squamish Public Library: Speaker Series: Exploring the Role of the Hippocampus in Spatial Memory.
Canadian Center for Behavioral Neuroscience
Lethbridge, AB
Invited Talk
Summer Student Symposium: The hippocampus is not necessary for cued location or discriminative cue memory in a novel two-platform water task in rats.
2015
Quest University Canada
Squamish, BC
Quest Biomedical Journal Club
Malformations of the developing brain: an AKT3-FOXG1-Reelin network underlies defective migration in human focal malformations of cortical development.
Awards
2017
Quest University Canada
Squamish, BC
Distinction
This award is given to students whose senior thesis was the best in their graduating class.
2015
American Physiological Society
Atlanta, GA
Undergraduate Summer Research Fellowship
This fellowship was awarded to students who expressed the capability to design and conduct independent research projects. I earned the UGSR fellowship from APS to fund my research with the Prisk Lab at UCSD.
Other relevant projects
2015-2016
Quest University Canada
Squamish, BC
Techniques in Cellular and Molecular Biology
I worked in a molecular biology lab developing methods of cloning and purifying T7-RNAP by transforming competent E. coli cells, and designing riboprobes. Specific skills: Miniprep, plasmid design and cloning, competent cell generation, PCR, primer design, Gel electrophoresis of DNA, RNA, and proteins, protein purification, and construction of different buffers and growth media.
Biostatistics
I was responsible for professionally analyzing data of small island biodiversity collected by a tutor at Quest University Canada. Specific skills: Advanced programming in R, extensive knowledge of statistics.
Work & Service
2016-2017
Squamish Hospice Society
Squamish, BC
Volunteer
As a volunteer, my role is to speak to hospice patients or families to provide comfort and stand vigil with individuals during the last 72 hours of life if they do not have family currently with them.
2015-2017
Quest University Canada
Squamish, BC
Quest Molecular Biology Journal Club- Founder & President
I founded a club for students to seminar and communicate recent publications in molecular biology. The club was founded in September 2015, and now has 50 members.
Quest Pre-Medical Society- President
The premedical society is a club that aspires to guide students who are interested or pursuing a premedical track. As president, I have organized book-lending programs for study materials, databases of health professionals willing to be shadowed by students, and established a relationship with the faculty and admissions head to convey these resources to future QUC students.
Peer Tutor
I was a subject specialist for organic and inorganic chemistry, genomic medicine, statistics, and edited proposals to the research ethics board. I also tutored quantitative reasoning and rhetoric.
2014-2016
Urban Alpine
Squamish, BC
Ski Technician
At urban alpine, I worked to restore and maintain a rental fleet of skis, as well as freelance work.
2014
Quest University Canada Student Affairs
Squamish, BC
Staff member
I was hired as a summer employee and designed a curriculum for a summer program for high school students. I also organized housing and finances for the incoming class of 2015.
Skills & Techniques
Highly organized, and independently motivated
Strong communication skills
Strong background in quantitative reasoning and science
Programming and software experience in MATLAB, Flojo, R, Python, and SnapGene
Experience in behavioral techniques including aseptic technique, stereotactic surgery, and euthanasia
Expertise in stereology and confocal microscopy
Knowledge of wet-lab techniques including flow cytometry, gene cloning, plasmid design, PCR/qPCR, gel electrophoresis, cell transformation, IHC, western blotting, ELISA, and ICC
Proficiency in advanced techniques such as perfusion-assisted clearing of tissue, one-photon calcium imaging acute slice recording
Experience in cell culture and transfection of primary neurons and neuronal stem cells
Extracurricular Interests
Member of the women’s racing team with the San Diego Bicycling Club
Member of the Mysterious Galaxy Writers’ group
Enthusiastic skier, rock-climber, and painter.
References available upon request
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