Julian Ostrovsky, M.Sc.
Summary of Qualifications
Multifaceted researcher with proven record of impacts and knowledge spanning human clinical translation studies, cancer biology, mitochondrial diseases, regulatory mechanisms of cell differentiation, and regeneration in variable disorders. Lab Operations Manager, Lab Manager, Lab Biosafety Officer.
Human Translation Studies
Human Genetics, Cancer, Metabolic Diseases and Therapy
Human Primary Tissues & Human Cell Culture
Embryonic & Cancer Tissue and Cells
Cell Control, Differentiation and Regeneration
C. Elegans, Mouse and Cell Models
Cell based& Biochemical Assays, ELISA, IB
Infections, Viruses, Transfections, Cloning
Radiolabeled compounds, Radioactive Labeling
“GE Amersham Biosciences” FACS, “AKTA” FPLC, “Beckman-Coulter” HPLC system, BiaCore3000 Surface Plasmon Resonance
OROBOROS O2k-high-resolution respirometry
Accuri C6 (BD Biosciences), BioSorter
Cell & Growth Factors & Receptors
RNA(RT-PCR, siRNA/shRNA,/DNA/Proteins & Antibodies
Microscopy: Live & Immunofluorescent Imaging
Research Design and Methods Development
Lab Operations Manager & Lab Manager, Safety Biosafety Officer
Safety Regulations Inventory & Supply and Equipment Management, Orders
Data and New Method Implementation
Statistics, Reports, Manual Development
Research Presentations and Publications
Assistance, Lab Personal Coaching
Excellent Communication Skills
Biotech and Academic Lab Collaborations
Excellent oral and written communication skills in 3 languages (English, Hebrew, Russian)
Molecular Biology / Biochemistry Research
Clinical and Scientific Experience
Children’s Hospital of Philadelphia- Department of Human Genetics, PA 2007-present
Responsible for development of experimental design, performance, implementation of techniques. Extensive experience to troubleshoot and resolve technical experimental issues. Collaborations and consultations with academic labs and industrial companies. Data analysis and research presentations.
Lab Manager & Lab Equipment Supervisor. Responsible for management and maintenance of various laboratory equipment including, but not limited to microscopes, freezers, liquid nitrogen tanks, incubators, centrifuges, autoclaves, AKTA, HPLC, spectrophotometers, microscopes and etc. Ordering lab equipment, tech instruments, and supplies; financial order tracking. Maintained chemical, biohazard and safety regulation inventories.
Design and management of lab manuals and lab databases.
Assay development and implementation of new methods. Performance of cell based and biochemical assays.
Selective list of technical expertise: Extensive experience in human derived cells in culture, primary, cancer and cell lines, including RNA/DNA/protein extracts, growth, gene expression (viral, transfections, cloning). Proficient in C. elegans research: maintenance, life spans of mutants, drug treatments, mitochondria/protein/lipids/DNA/RNA extractions, and biochemical and molecular biology analyses. Research experience with various mouse models, including dissections, tissue isolations, organ collections, mitochondria/protein/DNA/RNA isolations and analyses. High level of expertise of experimental design and performance in GE Amersham Biosciences FACS, AKTA FPLC, “Beckman-Coulter” HPLC system, BiaCore3000 Surface Plasmon Resonance, Accuri C6 (BD Biosciences), OROBOROS O2k-high-resolution respirometry, BioSorter. Protein/DNA/RNA isolation. Immunoblotting, immunoprecipitation, RNA/DNA/Protein purification, RT-PCR, siRNA/shRNA, PCR, gel electrophoresis, ELISA. Immunocytochemistry, Immunohistochemical and Immunofluorescent staining.
üSuccessfully completed number of publications in high rated journals.
üDesigned and establish protocol for stable isotopic profiling of intermediary metabolic flux in developing and adult stage C. elegans.
üSuccessful tests and analyses of numerous compounds healing ability of human derived fibroblasts from patients towards establishing of disease treatment .
Children’s Hospital of Philadelphia-Philadelphia, PA 2004-2007
Protein Core Facility
Lab Manager & Lab Technical Equipment Manager. Design and managing lab manuals and databases. Assay development, protocols, implementation of new techniques. Supply and equipment management.
Research design, experimental performance and outcome data interpretation for Protein Core Clients. Specialist in protein and peptide isolation, purification, separation, protein- protein interaction, protein and peptide identification research. Various tissue and cell culture techniques: Cell culture growth, differentiation, immortalization, gene expression (viral or transfections, cloning), including embryonic stem cells, human primary cell cultures, cancer and cell lines. Cell sample preparation for immunoblotting, RT-PCR, PCR, immunocytochemistry. Immunohistochemical and Immunofluorescent staining techniques. Confocal Microscope, Super Resolution Microscope, Light and Florescent Microscopes.
Worked on and maintained: GE Amersham Biosciences AKTA FPLC, “Beckman-Coulter” HPLC system, BiaCore3000 Surface Plasmon Resonance. Experienced with Accuri C6 (BD Biosciences), BioSorter (Union Biometrica).
Managing safety regulations, chemical inventories. Handing radioactive isotopes, toxic chemicals/reagents, recombinant DNA, retro and lenti viruses, recombinant DNA and biohazard materials.
Institute of Technology-Haifa, Israel 1997-1999
M.Sc., Biological Science
Responsible for research design, experiment performance, data analysis, presentation, and preparation of data for publication. Resolved technical issues and assay development. Maintained lab equipment, managed tissue cultures, and ensured radiation safety.
Institute of Technology-Haifa, Israel 1994-1997
B.Sc., Biological Science
Institute of Technology-Haifa, Israel 1994-1997
Research Technician in Dr. Gadi Shuster laboratory
RNA extract from spinach leaves. Technical lab support.
M.Sc., Biological Science -Microbiology
Technion-Institute of Technology Haifa, Israel
B.Sc., Biological Science -Microbiology
Technion-Institute of Technology Haifa, Israel
10 Years of Service Award, The Children’s Hospital of Philadelphia, Philadelphia, PA 2014
5 Years of Service Award, The Children’s Hospital of Philadelphia, Philadelphia, PA 2005
Technion Dean’s Awards for “Excellent in Studies” 1997-2003
1.N-acetylcysteine and vitamin E rescue animal longevity and cellular oxidative stress in pre-clinical models of mitochondrial complex I disease.
Polyak E, Ostrovsky J, Peng M, Dingley SD, Tsukikawa M, Kwon YJ, McCormack SE, Bennett M, Xiao R, Seiler C, Zhang Z, Falk MJ.
Mol Genet Metab. 2018 Feb 23. pii: S1096-7192(18)30041-6. doi: 10.1016/j.ymgme.2018.02.013. [Epub ahead of print] PMID: 29526616
2.PARP-1 Inhibition Rescues Short Lifespan in Hyperglycemic C. Elegans And Improves GLP-1 Secretion in Human Cells.
Xia Q, Lu S, Ostrovsky J, McCormack SE, Falk MJ, Grant SFA. Aging Dis. 2018 Feb 1;9(1):17-30. doi: 10.14336/AD.2017.0230. eCollection 2018 Feb.
3.Propionyl-CoA carboxylase pcca-1 and pccb-1 gene deletions in Caenorhabditis elegans globally impair mitochondrial energy metabolism.
Chapman KA, Ostrovsky J, Rao M, Dingley SD, Polyak E, Yudkoff M, Xiao R, Bennett MJ, Falk MJ. J Inherit Metab Dis. 2017 Nov 20. doi: 10.1007/s10545-017-0111-x. [Epub ahead of print]
4.Mitochondrial function requires NGLY1.
Kong J, Peng M, Ostrovsky J, Kwon YJ, Oretsky O, McCormick EM, He M, Argon Y, Falk MJ. Mitochondrion. 2018 Jan;38:6-16. doi: 10.1016/j.mito.2017.07.008. Epub 2017 Jul 25.
5.Inhibiting cytosolic translation and autophagy improves health in mitochondrial disease.
Peng M, Ostrovsky J, Kwon YJ, Polyak E, Licata J, Tsukikawa M, Marty E, Thomas J, Felix CA, Xiao R, Zhang Z, Gasser DL, Argon Y, Falk MJ. Hum Mol Genet. 2015 Sep 1;24(17):4829-47. doi: 10.1093/hmg/ddv207. Epub 2015 Jun 3.
6.Pharmacologic targeting of sirtuin and PPAR signaling improves longevity and mitochondrial physiology in respiratory chain complex I mutant Caenorhabditis elegans.
McCormack S, Polyak E, Ostrovsky J, Dingley SD, Rao M, Kwon YJ, Xiao R, Zhang Z, Nakamaru-Ogiso E, Falk MJ. Mitochondrion. 2015 May;22:45-59. doi: 10.1016/j.mito.2015.02.005. Epub 2015 Mar 3.
7.Mitochondrial DNA variant in COX1 subunit significantly alters energy metabolism of geographically divergent wild isolates in Caenorhabditis elegans.
Dingley SD, Polyak E, Ostrovsky J, Srinivasan S, Lee I, Rosenfeld AB, Tsukikawa M, Xiao R, Selak MA, Coon JJ, Hebert AS, Grimsrud PA, Kwon YJ, Pagliarini DJ, Gai X, Schurr TG, Hüttemann M, Nakamaru-Ogiso E, Falk MJ. J Mol Biol. 2014 May 29;426(11):2199-216. doi: 10.1016/j.jmb.2014.02.009. Epub 2014 Feb 14.
8.In vivo metabolic flux profiling with stable isotopes discriminates sites and quantifies effects of mitochondrial dysfunction in C. elegans.
Schrier Vergano S, Rao M, McCormack S, Ostrovsky J, Clarke C, Preston J, Bennett MJ, Yudkoff M, Xiao R, Falk MJ. Mol Genet Metab. 2014 Mar;111(3):331-41. doi: 10.1016/j.ymgme.2013.12.011. Epub 2013 Dec 27.
9.Mutations in FBXL4, encoding a mitochondrial protein, cause early-onset mitochondrial encephalomyopathy.
Gai X, Ghezzi D, Johnson MA, Biagosch CA, Shamseldin HE, Haack TB, Reyes A, Tsukikawa M, Sheldon CA, Srinivasan S, Gorza M, Kremer LS, Wieland T, Strom TM, Polyak E, Place E, Consugar M, Ostrovsky J, Vidoni S, Robinson AJ, Wong LJ, Sondheimer N, Salih MA, Al-Jishi E, Raab CP, Bean C, Furlan F, Parini R, Lamperti C, Mayr JA, Konstantopoulou V, Huemer M, Pierce EA, Meitinger T, Freisinger P, Sperl W, Prokisch H, Alkuraya FS, Falk MJ, Zeviani M. Am J Hum Genet. 2013 Sep 5;93(3):482-95. doi: 10.1016/j.ajhg.2013.07.016. Epub 2013 Aug 29.
10.Primary respiratory chain disease causes tissue-specific dysregulation of the global transcriptome and nutrient-sensing signaling network.
Zhang Z, Tsukikawa M, Peng M, Polyak E, Nakamaru-Ogiso E, Ostrovsky J, McCormack S, Place E, Clarke C, Reiner G, McCormick E, Rappaport E, Haas R, Baur JA, Falk MJ. PLoS One. 2013 Jul 24;8(7):e69282. doi: 10.1371/journal.pone.0069282. Print 2013.
11.NMNAT1 mutations cause Leber congenital amaurosis.
Falk MJ, Zhang Q, Nakamaru-Ogiso E, Kannabiran C, Fonseca-Kelly Z, Chakarova C, Audo I, Mackay DS, Zeitz C, Borman AD, Staniszewska M, Shukla R, Palavalli L, Mohand-Said S, Waseem NH, Jalali S, Perin JC, Place E, Ostrovsky J, Xiao R, Bhattacharya SS, Consugar M, Webster AR, Sahel JA, Moore AT, Berson EL, Liu Q, Gai X, Pierce EA. Nat Genet. 2012 Sep;44(9):1040-5. doi: 10.1038/ng.2361. Epub 2012 Jul 29.
12.Probucol ameliorates renal and metabolic sequelae of primary CoQ deficiency in Pdss2 mutant mice. Falk MJ, Polyak E, Zhang Z, Peng M, King R, Maltzman JS, Okwuego E, Horyn O, Nakamaru-Ogiso E, Ostrovsky J, Xie LX, Chen JY, Marbois B, Nissim I, Clarke CF, Gasser DL. EMBO Mol Med. 2011 Jul;3(7):410-27. doi: 10.1002/emmm.201100149. Epub 2011 Jun 8.
13.Stable isotopic profiling of intermediary metabolic flux in developing and adult stage Caenorhabditis elegans.
Falk MJ, Rao M, Ostrovsky J, Daikhin E, Nissim I, Yudkoff M. J Vis Exp. 2011 Feb 27;(48). pii: 2288. doi: 10.3791/2288.
14.Mitochondrial respiratory chain dysfunction variably increases oxidant stress in Caenorhabditis elegans. Dingley S, Polyak E, Lightfoot R, Ostrovsky J, Rao M, Greco T, Ischiropoulos H, Falk MJ. Mitochondrion. 2010 Mar;10(2):125-36. Epub 2009 Nov 10.
Children’s Hospital of Philadelphia-Philadelphia, PA 2005-present
Department of Human Genetics
Trained postdoctoral fellows, lab personal, graduate students in Dr. Marni Flak’s laboratory.
Mentoring responsibilities included providing guidance on bio hazardous material in the lab, training and supervision of students/postdocs on use of lab equipment, teaching biological techniques for experimental performance and analyzing of outcomes.
Michael J. Bennett, PhD, FRCPath, FACB
Director, Michael J. Palmieri Metabolic Laboratory, CHOP
Professor of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine
Michael J. Palmieri Metabolic Lab
The Children's Hospital of Philadelphia
Main Building, 5th floor
3401 Civic Center Boulevard
Philadelphia, Pennsylvania 19104
Marc Yudkoff, MD
Chief, Division of Child Development, Rehabilitation and Metabolic Disease
The Children’s Hospital of Philadelphia
34th & Civic Center Boulevard
Additional references can be provided upon request.