Medical Data
Resume:
Sayeda Nasrin Alam
Westford, MA 01886
Cell: 978-***-****
ac4x66@r.postjobfree.com
Professional Summary:
Strong scientific background and animal laboretory experience in a broad range of specialty in Medicine which includes gastrointestinal pathology, metabolic syndrome, obesity, thyroid problems, nuroendocrine and reproductive endocrine defects. My research focuses on the role of the brush-border enzyme Intestinal Alkaline Phosphatase(IAP) in the maintenance of normal homeostasis of intestinal microbiota, which is also relate to obesity, Type 2 diabetes, insulin resistance and dyslipidemia. Other than scientific works, responsible to manage all animal project related work, study design, protocol implementation and collaborate all animal works with other fellows. Extensive experience in pre clinical animal study; design and perform animal surgeries and procedures, necropsy, serum collection by using differenrt methods. Developed murine model in mouse and rat as well as responsible for rodent’s colony management and regular husbandry. Excellent data presentation skill using powerpoint, strong computer literacy and scientific graphing and data analysis skills using Excel, Sigma Plot and GraphPad Prism.
Technical Expertise:
DNA/RNA extraction and purification, gel electrophoresis, DNA genotyping.
Molecular analyses including PCR, RT-PCR, western blotting, High-performance liquid chromatography Small and large scale preparation of plasmid DNA, restriction digestion, transformation and DNA cloning.
Small and large scale preparation of plasmid DNA, restriction digestion, transformation and DNA cloning.
Mammalian Cell culture, transfection, generation of stable cell lines
Luciferase reporter assay, protein assays, alkaline phosphatase assay
Enzyme-linked immunosorbent assay (ELISA)
Flow Cytometry
Spectrophotometry
Immunohistochemistry.
Work Experiences:
Harvard Medical school, July, 2012 - Present
Research Technologist (Animal Project)
Lab of Dr. Stephanie Seminara; Reproductive Endocrine Unit Massachusetts General Hospital
I have worked on developing scientific mouse model to study reproductive neuroendocrine pathway that are responsible for infertility. Both mice and men with mutations in the genes encoding kisspeptin and its receptor (Kiss1/KISS1 and Kiss1r/KISS1R) have severe hypogonadotropic hypogonadism, although both species demonstrate evidence for modest kisspeptin-independent GnRH secretion. In contrast, humans with mutations in the neurokinin B (NKB) signaling pathway (TAC3 and TACR3) have less severe hypogonadotropism than their kisspeptin counterparts as evidenced by reversal of their GnRH-deficient state. Furthermore, mice lacking the neurokinin B receptor (encoded by Tacr3) exhibit normal timing of sexual maturation but have subfertility in adulthood. As the reproductive phenotype of mice carrying mutations in Tac2, which encodes the rodent form of NKB, has not yet been described, I examined the reproductive phenotype of these animals in detail.
Responsibilities:
Responsible to manage the entire lab, daily lab inventory and ordering entire lab supplies, making all reagents, provide training and supervising others in laboratory techniques, collaborate all animal works with other fellows and mentoring summer students.
Data analysis and Data presentation using Excel, Powerpoint, Sigma Plot and GraphPad Prism.
Mammalian cell culture, maintaining cell lines
Responsible for maintaining rodents colony, design and perform animal surgeries and procedures.
DNA/RNA extraction and purification, gel electrophoresis, DNA genotyping.
Molecular analyses including PCR, RT-PCR
Enzyme-linked immunosorbent assay (ELISA)
Flow Cytometry
Spectrophotometry
immunohistochemistry
Harvard Medical school, August, 2007 - 2012
Boston, MA
Research Technologist
Lab of Dr. Richard A. Hodin, Gastrointestinal Epithelial Biology
Massachusetts General Hospital
I have worked on multiple projects that explore the role of Intestinal Alkaline Phosphatase (IAP) in autoimmune cause of Inflamatory Bowel Disease, in the regulation of fat absorption, gut defense mechanism and maintaining the normal homeostasis of gut microbiota. Dysregulation of the normal homeostasis of intestinal microbiota has been implicated in the pathogenesis of a myriad of disease conditions including gastrointestinal infections, autoimmune inflammations (IBD) and obesity.The hypothesis is that IAP is the major regulating factor of maintaining normal gut flora and protects us from potential harmful abnormal immunological effects, through a mechanism, which is poorly understood.
Responsibilities:
Mammalian Cell culture, transfection, generation of stable cell lines
Small and large scale preparation of plasmid DNA, restriction digestion, transformation and DNA cloning.
DNA/RNA extraction and purification, gel electrophoresis, DNA genotyping.
Molecular analyses including PCR, RT-PCR, western blotting, High-performance liquid chromatography
Designed and conducted research studies to explore the role of Intestinal Alkaline Phosphatase (IAP) for prevention of Salmonella and Clostridium difficile infection in murine model. Responsible for maintaining rodents colony, design and perform animal surgeries and procedures.
For in vitro experiment, using different cell lines mostly human colon cancer cells and for in vivo experiment, using IAP wild type and IAP knockout mice.
Enzyme-linked immunosorbent assay (ELISA)
Responsible to manage the entire lab, daily lab inventory and ordering entire lab supplies, making all reagents, provide training and supervising others in laboratory techniques, collaborate all animal works with other fellows and mentoring summer students.
Data analysis and Data presentation using Excel, Powerpoint, Sigma Plot and GraphPad Prism.
Responsible for maintaining rodents colony, design and perform animal surgeries and procedures
Education, Licensure and Certifications:
Federation of State Medical Boards, U.S.A. Educational Commission for Foreign Medical Graduates (ECFMG Certified, U.S.A.)
Bachelor of Medicine and Bachelor of Surgery (MBBS), Chittagong Medical College, Bangladesh
References Available Upon Request
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