CURRICULUM VITAE

SNEHA SACHAN

Mob: 91-895*******

E-mail-id: ac3d4w@r.postjobfree.com

Objective:

A challenging research position in a research and development organization to advance my

Capabilities and take up increasing responsibilities to achieving set goals of the organization.

Academic Profile:

Qualifications

Specialization

Board/Univ

Year

CGPA/%age

M.Sc.

BIOTECHNOLOGY

BABASAHEB BHEEMRAO AMBEDKAR UNIVERSITY

2016

67%

Graduation

BIOTECHNOLOGY

CSJM UNIVERSITY

2013

60%

12th

BIOLOGY

U.P BOARD

2009

62%

10th

SCIENCE

U.P BOARD

2007

58%

DBT-BCIL Industrial Training

Company Name: MRD Life Sciences Pvt. Ltd. Lucknow.

Duration: SIX Month (From November 2016 to April, 2017)

Technical Skill Sets

Microbiology

Molecular Biology

Media Preparation & Culturing of Microbes.

Pure Culture of Microbes through Streaking Method.

Primary & Secondary Screening of Microbes.

Growth Kinetic Studies of Microbes.

Antibiotics Sensitivity Test.

Minimum Inhibitory Concentration (MIC) Test.

MDR Test.

Fermentation & Downstream Processing (DSP).

Genomic DNA Isolation from Microbes.

& Plant Sample.

Agarose Gel Electrophoresis for Genomic DNA.

RNA Isolation from Plant Samples.

Denaturing Gel Electrophoresis of RNA.

Qualitative Analysis of Nucleic Acid.

Quantitative Analysis of Nucleic Acid.

Southern/Northern Blotting.

Polymerase Chain Reaction (PCR).

Electrophoresis of PCR Products.

Qualitative analysis of phytochemical

Biochemical Techniques & Proteomics

Recombinant DNA Technology (RDT)

Protein Isolation & Buffer Preparation.

SDS-PAGE.

Estimation of Protein by Bradford Method.

Estimation of Protein by Lowry’s Method.

Enzyme Assays.

Chromatography Techniques.

HPLC.

Restriction Digestion.

Ligation.

Competent Cell Preparation.

Transformation.

Blue-White Screening.

Cloning & Gene expression

Hands-on Instrumentation

Basic Equipment’s

Hot Air Oven.

Bacterial Incubator.

Shaker Incubator.

Auto-clave.

Water Bath.

Laminar Air Flow.

Distillation Unit.

Digital Weighing Balance.

Magnetic Stirrer.

Vortex Mixer.

Digital pH Meter.

Electrophoretic Apparatus- SDS PAGE, Agarose Gel.

BOD Incubator.

Microscope.

Soxhlet Apparatus.

Blotting Apparatus.

Hot Plate Stirrer.

Centrifuge Machine.

UV Trans illuminator.

White Light Trans illuminator.

Micro-pipettes.

Advanced Equipment’s

Thermal Cycler-96 Wells (ABS).

Gel Doc System (Major Science).

HPLC (Shimadzu).

GC (Bio-Rad).

RT-PCR (Bio-Rad

UV- Visible Spectrophotometer.

Colorimeter.

Micro-controller pH Meter.

Fermenter.

Project profile

Project Titled 1:

College Name:

Duration:

Abstract:

“PCR” use as a diagnostic tool for identification of beta thalassemia in north Indian population.

Babasaheb Bhimrao Ambedkar University, Lucknow

five month(January to may2016)

The present investigation was carried out to understand about beta

thalassemia which is autosomal hereditary blood disorder which reduces the

Production of hemoglobin and cause anemia. Major objective of this investigation is to diagnose beta thalassemia in north Indian population by polymerase chain reaction.in this diagnosis 1)isolated the blood DNA of by salting out method .2) DNA separated by Agarose gel electrophoresis.3) use exon 2 specific beta globin primer and amplify the DNA by polymerase chain reaction. We obtained a 632bp band which is amplified product of normal beta globin gene chain. We used an exon 2 specific primer of normal beta globin gene which is amplified the exon 2 region of beta globin chain

Because of no mutation we obtained a 632bp band which shows that there

is no mutation present in normal person.

Keywords: Beta thalassemia, DNA isolation, polymerase chain reaction.

Project Titled 2

Company Name:

Duration:

Abstract:

“Extraction of secondary metabolites from fresh leaf, stem and root of Cajanus cajan (Arhar) plant.

MRD Life Sciences Pvt. Ltd., Luck now.

Six Month (From November 2016 to April, 2017).

Cajanus cajan plant collected from agriculture field, luck now for the extraction of secondary metabolites. 1) Cajanus cajan plant were taken and separated into each part. After that each part washed properly and weighing for solvent extraction method. All parts are dipped into different solvent. After that we obtained extract of each part and then we check the antibiotic activity by antibiotic sensitivity test against different pathogen.2) After that we do thin layer chromatography to check the presence of compound in each extract by using different solvent. 3)After doing this we done column chromatography to separate compound which is present in extract of leaf of Cajanus cajan plant by using 80%methanol solvent then we do 4)qualitative analysis of secondary metabolites which is shown the presence of flavonoids, carbohydrate and tannins. Then lastly we check the 5) minimal inhibitory concentration of our extract by using a B.subtilis pathogen at 620nm wavelength.

Key words: Solvent extraction method, Antibiotic sensitivity test, Thin layer chromatography, Column Chromatography, Qualitative Analysis of phytochemical, Minimal inhibitory concentration.

Computer Proficiency

BasicKnowledge:

Software:

Database:

MS word, Power Point, Excel, Photoshop, Internet etc.

BLAST, FASTA, PRIMER DESIGNING

NCBI, MiRBase

Key Competencies

Honest.

Quick Learner.

Optimistic.

Result Oriented Approach.

High Energy Level.

Self Confidence.

Punctual.

Leadership.

Special Achievements

Attended seven Days Hands-on Workshop on high performance liquid chromatography (HPLC) at MRD Life Sciences Pvt. Ltd., Luck now held on March 04-10jan, 2017.

Attended Three Days Hands-on Workshop on Bioprocessing (Fermenter) at MRD Life Sciences Pvt. Ltd., Luck now held on March 20-22, 2017

Attended three days International conference on updates in “Cancer prevention and research (ICUCPR)” at Babasabheb Bhimrao Ambedkar university, Luck now held on 14-16 Febuary 2017.

Two month’s summer training at Nucleus Biozone pvt. Ltd. On April to June 2012.

Personal Details

Hobbies:

Father’s Name:

DOB:

Marital Status:

Sex:

Language Known:

Nationality:

Permanent Address:

Playing badminton, cooking, reading books/magazines & listening music.

Mr. Anil Sachan

15 July 1994

Unmarried.

Female.

Hindi, English

Indian.

B-4/90 vijyant khand,lucknow(U.P.), INDIA.

References

Mr. Manoj verma

Director of MRD Life Sciences pvt. ltd.

Lucknow

Mob.no. 983-***-****

Email.id: ac3d4w@r.postjobfree.com

Few More

On Demand

Declaration: I do here by declare that all the details furnished above are complete and correct to the best of my knowledge.

Date:

Place SNEHA SACHAN

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