Lívia Viganor, MSc, PhD

**** * ******** **, ****, Phoenix, AZ 85054 Nationality: Brazilian and Italian (L2 - EAD Visa) https://www.linkedin.com/in/liviaviganor/ +1-480-***-**** ac3963@r.postjobfree.com

PERSONAL STATEMENT

Expert in Microbiology, Biochemistry, Biotechnology and Molecular Biology due to a strong background acquired during post-graduation degrees in Microbiology and Pharmacy undergraduate course in Brazil. More recently, improved research and professional experience during a Postdoctoral Fellowship in Ireland, with a main focus of the investigation of novel metal-based therapeutics against multidrug resistant bacteria. The scientific and biotechnological interests are based on excellent interpersonal and communication skills, combined with ambition, determination, focus and high degree of personal organization, plus self-motivation and readiness to take on responsibilities.

PROFESSIONAL EXPERIENCE

●May/2017 – Nov/2017: Microbiologist / Quality inspector

West Pharma - Contract Manufacturing, Dublin

-Coordinated daily duties, executing the tasks whilst applying high standards of GMP and GLP

-Performed microbiological testing and provided support the site in deviation and laboratory investigations

-Provided technical guidance and expertise to QC Microbiology Team and external departments

-Executed QC-micro testing and environmental monitoring in compliance with cGMP, EU regulatory requirements and Standard Operating Procedures

-Supported the management of GMP documentation, inputting data into and generate reports from quality databases

-Participated in training activities in the lab

-Participated in validation and re-validation of techniques, procedures, equipment, cleanrooms and laboratories

-Achievements: Performance qualification of cleanrooms ISO class 8 - Grade C/D in operation (Environmental/Personnel monitoring & non-viable/viable particulate monitoring)

> Evaluation of airborne non-viable particulates concentration

> Microbial identification of morphologically different representative isolates recovered from active air and surfaces

> Documented pressure reading, printout of temperature and relative humidity

-Assured that manufactured items were made and assembled correctly

-Approved finished products by confirming specifications; conducting visual and measurement tests

-Documented inspection results by completing reports and logs; summarizing product check back and scrap; inputting data into quality database

-Kept measurement equipment operating by following operating instructions

-Maintained safe and healthy work environment by following standards and procedures; complying with legal GMP and EU regulations

-Attended training courses ; reading updated work instructions and SOPs

-Achievements: Accomplished quality and organization tasks by completing inspections, measurement tests and documentation as needed.

●Sep/2015 – Apr/2017: Assistant lecturer in Microbiology Practical Programme

School of Biological Sciences - Dublin Institute of Technology (DIT)

-Conducted laboratory practical sessions of:

> Microbial Growth and Genetics for BSc Biosciences (Year 2); Introduction to Microbiology for BSc Biomedical Sciences (Year 1); Microbiology & Society for BSc Sciences, Forensic and Environmental Chemistry and Chemical Sciences and Medical Chemistry (Year 1); Introduction to Infection and Immunity for BSc Biomedical and Molecular Diagnostics (Year 1); Microbial Diagnostics for Biosciences (Year2)

-Lectured main topics of:

> Introduction of aseptic technique for the safe handling of micro-organisms

> Provision of basic knowledge on the different types of microorganisms, their manner of existence and their beneficial and detrimental effects on society

> Development of an understanding of the principles and methodology employed for the isolation, identification and antimicrobial susceptibility testing of clinically significant pathogens and the role of serological techniques in the diagnosis of infectious disease

-Achievements: Learned a lot through teaching and implemented the programme that allowed the students to consolidate subject knowledge, enhance the learning of scientific knowledge, develop technical, cognitive and teamwork skills

●Sep/2014 – Jan/2017: Postdoctoral research fellow

FOCAS Institute, DIT

-Undertook the main research project entitled: "Effects of 1,10-phenanthroline and its derivatives on multiple biological processes of Pseudomonas aeruginosa"

-Embraced a part-time lecturing role in the School of Biological Sciences and provided project/laboratory supervision to undergraduate and postgraduate students.

-Successfully applied and approved for PhD scholarship and research project funding in DIT (approx. € 80,000)

-Published 4 papers and 1 book chapter, presented research results at 3 Latin American conferences and 2 major European conferences in the fields of microbiology and biological inorganic medicinal chemistry.

-Worked very closely with the Technology Transfer Office in DIT to develop knowledge and capabilities in the areas of intellectual property protection and research commercialization.

-Played a pivotal role in further developing the on-going research collaboration involving the Brazilian and Irish members of the Metal-Based Therapeutics research consortium established under the Research Brazil-Ireland (RBI) initiative.

-Involved in key activities such as:

> Improving workflow and lab productivity; developing/validating protocols and techniques; equipment maintenance; performing biological tests and sample preparations based on SOP s and specific requirements according to GMP/GLP regulations; preparing technical reports and publishing research papers

> Isolation, identification and maintenance of different microorganism specimens; antimicrobial susceptibility testing; bioburden and endotoxin testing; environmental monitoring; disinfection and sterilization of materials and equipment

> Protein extraction; enzymatic activity measurements, spectrophotometric and fluorimetric assays; flow cytometry analysis; DNA and rRNA extraction; cDNA preparation for real-time PCR analysis; gene cloning, protein expression and purification; site directed mutagenesis; in vivo analysis using the Galleria mellonella larvae model for toxicity screening of potential metal-based therapeutics.

●Dec/2004 – Aug/2006: Pharmacist

Bioderma Pharmacy, Vitória, Espírito Santo, Brazil

-Interpreted and established environment conditions in compliance with relevant legislation.

-Supervised and assured proper acquisition, conservation and dispensing of drugs and other products.

-Participated in pharmacovigilance studies based on analysis of adverse reactions and drug interactions, informing the local health authority.

-Maintained updated database and documentation of products subjected to special control.

-Evaluated medical prescriptions and provided pharmaceutical assistance to clients.

-Organized areas, displays and activities of the pharmacy and provided staff training.

-Achievements: Increased prescription count by an average of 11% per year through increased customer service and pharmacy efficiency.

EDUCATION

●Mar/2010 – Mar/2014: Doctor of Philosophy (PhD) in Microbiology

Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

Thesis: Production of virulence attributes in clinical strains of Pseudomonas aeruginosa: effects of 1,10-phenanthroline and its derivatives on multiple biological processes

●Mar/2013 – Aug/2013: Sandwich PhD Program at Dublin City University (DCU)

Followed by a short collaborative research period at Maynooth University (MU)

●Mar/2007 – Mar/2009: Master Science (MSc) in Biotechnology

Federal University of Espírito Santo (UFES), Espírito Santo, Brazil

Dissertation: Evaluation of synergistic potential of vancomycin combined to other antimicrobial against Staphylococcus aureus and Staphylococcus coagulase-negative strains resistant to oxacillin

●Nov/2005 – Jan/2007: Basic and Clinical Pharmacology Specialization

Federal University of Espírito Santo (UFES), Espírito Santo, Brazil

Monograph: Drug interactions between antiretroviral and anticancer therapies

●Feb/2001 – Jul/2005: Bachelor Science (BSc) in Pharmacy (habilitation in Biochemistry) Higher School of Health Sciences (EMESCAM), Espírito Santo, Brazil

Undergraduate final project: Via Transdermal for Drug Administration

PUBLICATIONS

●Anna Clara M. Galdino, Marta H. Branquinha, André L.S. Santos, Lívia Viganor. Pseudomonas aeruginosa and its arsenal of proteases: weapons to battle against host. Book titled: Pathophysiological Aspects of Proteases. Series titled: Advances in biochemistry in Health and Disease, Springer, 2016. [Book chapter accepted for publication].

●Viganor L, Howe O, McCarron P, McCann M, Devereux M. The antibacterial activity of metal complexes containing 1,10-phenanthroline: potential as alternative therapeutics in the era of antibiotic resistance. Current Topics in Medicinal Chemistry. 2016. Review. [Accepted for publication]

●Galdino AC, Viganor L, Ziccardi M, Nunes AP, Dos Santos KR, Branquinha MH, Santos AL. Heterogeneous production of proteases from Brazilian clinical isolates of Pseudomonas aeruginosa. Enferm Infecc Microbiol Clin. 2016.[Epub ahead of print]

●Viganor L, Galdino AC, Nunes AP, Santos KR, Branquinha MH, Devereux M, Kellett A, McCann M, Santos AL. Anti-Pseudomonas aeruginosa activity of 1,10-phenanthroline-based drugs against both planktonic- and biofilm-growing cells. J Antimicrob Chemother. 2016; 71(1): 128-34

●Viganor L, Skerry C, McCann M, Devereux M. Tuberculosis: An Inorganic Medicinal Chemistry Perspective. Curr Med Chem. 2015; 22(18): 2199-224. Review

●Silva LV, Galdino AC, Nunes AP, dos Santos KR, Moreira BM, Cacci LC, Sodré CL, Ziccardi M, Branquinha MH, Santos AL. Virulence attributes in Brazilian clinical isolates of Pseudomonas aeruginosa. Int J Med Microbiol. 2014; 304(8): 990-1000

●Santos AL, Sodre CL, Valle RS, Silva BA, Abi-Chacra EA, Silva LV, Souza-Goncalves AL, Sangenito LS, Goncalves DS, Souza LO, Palmeira VF, d'Avila-Levy CM, Kneipp LF, Kellett A, McCann M, Branquinha MH. Antimicrobial action of chelating agents: repercussions on the microorganism development, virulence and pathogenesis. Curr Med Chem. 2012; 19(17): 2715-37. Review

●Silva LV, Araújo MT, Santos KR, Nunes AP. Evaluation of the synergistic potential of vancomycin combined with other antimicrobial agents against methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus spp strains. Mem Inst Oswaldo Cruz. 2011; 106(1): 44-50

MENTORING AND SUPERVISION OF STUDENTS

●Feb/2016 – Current: Student: Megan O’Shaughnessy

School of Biological Sciences, DIT, Ireland

PhD thesis (supervisor): The antimicrobial and anti-inflammatory effects of novel metal-based complexes.

●Aug/2015 – Current: Student: Anna Clara Milesi Galdino.

Postgraduate Programme in Biochemistry, UFRJ, Brazil

PhD thesis (co-supervisor): Investigation of anti-Pseudomonas aeruginosa mechanisms of action of 1,10-phenanthroline derivatives compounds.

●Jan/2016 – Mar/2016: Student: Sofie Sundkvist

Erasmus+ Programme, DIT, Ireland

Undergraduate final project (supervisor): Effects of 1,10-phenanthroline and its derivatives on elastase B of Pseudomonas aeruginosa.

●Aug/2013 – Aug/2015: Student: Anna Clara Milesi Galdino

Postgraduate Programme in Biochemistry, UFRJ, Brazil

MSc thesis (co-supervisor): Antibiotic and anti-virulence activities of silver coordinated compounds derived from 1,10-phenanthroline on Pseudomonas aeruginosa.

RESEARCH GRANTS AND AWARDS

●Dec/2014: Postdoctoral Fellowship (Brazilian Researchers Mobility Programme)

Awarding body: Science without Borders

Project’s Researcher: Lívia Viganor

Project’s Title: Effects of 1,10-phenanthroline and its derivatives on the multiple biological processes of Pseudomonas aeruginosa

●Jun/2015: RAP 1 - Seed Funding Scheme

Awarding body: Dublin Institute of Technology (DIT)

Project’s Researcher: Lívia Viganor

Project’s Title: Effects of 1,10-phenanthroline and its derivatives on elastase B of Pseudomonas aeruginosa

●Dec/2015: Fiosraigh Scholarship Programme – Dean of Graduate Students Award

Awarding body: Dublin Institute of Technology (DIT)

Research Student: Megan O’Shaughnessy

Project’s Title: The antimicrobial and anti-inflammatory effects of novel metal-based complexes

TRAINING AND COURSES

●Dec/2015: Emergency First Aid Training. Certified by National Safety Centre

●Jun/2015: Emergency Response Training. Certified by Staff Training & Development – DIT

●Jun/2015: Manual Handling. Certified by Staff Training & Development – DIT

●Oct/2014 – Jul/2015: Part-time Advanced English Course. Atlas Language School, Dublin, Ireland

●2016: 13th European Biological Inorganic Chemistry Conference (EuroBIC 13), Budapeste, Hungria

Poster: Effects of 1,10-phenanthroline and its derivatives on the elastase B activity and expression of Pseudomonas aeruginosa

●2016: IV International Conference on Antimicrobial Research (ICAR2016), Malaga, Spain

Oral presentation: Elastase secretion profile from clinical Brazilian isolates of Pseudomonas aeruginosa: effects of 1,10-phenanthroline and its derivatives on the enzymatic activity and expression.

●2015: 23rd International Congress of the IUBMB and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq), Iguazu Falls, Brazil

Poster: Differential protease production by Brazilian clinical strains of Pseudomonas aeruginosa: Effects of 1,10-phenanthroline and its derivatives on elastase B activity

●2015: III Symposium (IV Workshop) in Research, Development and Innovation for Bacterial and Fungal Diseases, Rio de Janeiro, Brazil

Poster: Silver compounds derived from 1,10-phenanthroline: a promising alternative therapy against Pseudomonas aeruginosa

●2015: 1st Brazil-Ireland Science Week, Dublin, Ireland

Poster: Effects of 1,10-phenanthroline and its derivatives on multiple biological processes in Pseudomonas aeruginosa

●2014: XXII Latin-American Conference in Microbiology and 4 Colombian Conference in Microbiology, Cartagena, Colombia

Poster: Effects of silver coordinated compounds on different biological processes of Pseudomonas aeruginosa

●2013: I Scientific Meeting Brazil-Ireland, Rio de Janeiro, Brazil

Oral presentation: Effects of 1,10-phenanthroline and its derivatives on multiple biological processes of Pseudomonas aeruginosa.

●2013: 65th Irish Universities Chemistry Colloquium, Dublin, Ireland

Poster: Metal-based Antimicrobial Protease Inhibitors

●2012: 28 Microbial Genetics Meeting, Iguazu Falls, Brazil

Poster: Proteolytic and genotypic profiles of different clinical samples of Pseudomonas aeruginosa

●2010: 44º Brazilian Congress of Clinical Pathology and Laboratorial Medicine, Rio de Janeiro, Brazil

Poster: Evaluation of antimicrobial activity of metalloprotease inhibitors against multidrug-resistant Pseudomonas aeruginosa

●2010: XX Latin-American Conference in Microbiology and IX National Microbiologist Meeting, Montevideo, Uruguay

Poster: Evaluation of antimicrobial activity of metalloprotease inhibitors against multidrug-resistant Pseudomonas aeruginosa

ADDITIONAL INFORMATIONS

●Ad hoc reviewer (journal): Infectious Diseases – Infect Dis (Lond)

●Advanced Computer skills: Microsoft Office (Word, Excel, Power Point); GraphPad Prism, GelCompar II, Mascot-Matrix Science, 7500 Real-time PCR Software, BD Accuri C6.

●Language skills: Portuguese (native), English (advanced written and verbal communication), Italian and Spanish (verbal comprehension)

REFERENCES UPON REQUEST

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