Technical Specialist
Resume:
Mark Hubbard
Saint Paul, MN 55106
**********@*****.***
SUMMARY
I have over ten year’s experience in the Clinical Lab Science profession, working in multiple departments within Mayo Clinic. Each of these labs have given me a vast knowledge base as well as experience in multiple disciplines within clinical lab sciences. Most recently I have continued to work with and gain significant knowledge in variant annotation for large genetic panels on NGS platforms.
EXPERIENCE
Technical Specialist - Variant Annotation, Molecular Hematopathology, Mayo Clinic
Rochester, MN - November 2014 to Current
Variant analysis, classification, and interpretation primarily for Next Generation Sequencing. Working in teams alongside Biostatistics, Bioinformatics, and Genetic Counselors to result out large panel testing on the Next Generation Illumina platforms.
Clinical Technologist, Molecular Hematopathology, Mayo Clinic
Rochester, MN - April 2014 to November 2014
Experience in PCR testing from start to finish, DNA/RNA extraction, PCR setup, PCR Sequencing, ABI utilization, Sequencing analysis and interpretation.
Clinical Technologist, Endocrine Laboratory, Mayo Clinic
Rochester, MN - September 2009 to April 2014
Experience in organic phase extraction/preparation, Mass Spectrometry, Immunoassay Dialysate, and Genomic panel testing.
Clinical Technologist, Clinical Core Laboratory, Mayo Clinic
Rochester, MN - May 2007 to August 2009
Medical lab science core technician for Hematology, Coagulation, Chemistry, and Immunology. Specialized in maintenance of Roche Chemistry diagnostic instruments.
Student Technologist - Biochemical Genetics Lab, Summer Lab Student Program, Mayo Clinic
Rochester, MN - May 2006 to August 2006
Summer Lab technician preparing samples for GC/MS analysis for inborn errors in the metabolic pathways.
EDUCATION
Iowa State University, Ames, IA
Bachelor of Science, Biochemistry 2003 - 2007
Undergrad research in Skeletal Muscle lab studying interaction of Intermediate Filaments
SKILLS
●PCR Amplification
●DNA/RNA primer and probe design
●Sanger Sequence Analysis - Mutation Surveyor, Sequencher, Seq Scanner
●Multiplex Ligation-dependent Probe Amplification (MLPA)
●Gel Electrophoresis
●DNA and RNA extraction
●Pipetting
●Thermocycler general use and maintenance
●Pipetting Automation - Hamilton/Tecan/Qiagen
●Integrative Genomics Viewer (IGV)
●Alamut Visual
●Variant annotation per ACMG guidelines
●ABI general use and maintenance
●Excel/PowerPoint/Word
REFERENCES
David Viswanatha, M.D. –Lab Director for Molecular Hematopathology/Pathologist, Mayo Clinic-RST
**********.*****@****.*** 507-***-****
Rong He, M.D. – Lab Director for Molecular Hematopathology/Pathologist, Mayo Clinic-RST
**.****@****.*** 507-***-****
William G. Morice II, M.D., Ph.D – Chair of the Department of Laboratory Medicine and Pathology, Mayo Clinic-RST
******.*******@****.*** 507-***-****
Contact this candidate