Dr. Jitcy Saji Joseph, PhD

ac2t79@r.postjobfree.com

Gordon valley, Florida Glen, Johannesburg 1712, South Africa Cell: +27-83-290-****

https://scholar.google.co.za/citations?user=jGOdGJUAAAAJ&hl=en https://www.linkedin.com/in/dr-jitcy-joseph-4518a153/ Highly motivated and innovative scientist/lecturer with specialization in molecular biology, cell biology, metabolism, physiology, analytical chemistry and biochemistry. Expertise in development and execution of genetic and biochemistry-based research methods. Passionate about learning new scientific skills. Enthusiastic team player adept at providing leadership while also learning from fellow team members. Experienced in managing multiple projects simultaneously. Effective communicator and teacher that can conceptualize complex biological to audiences with varied expertise. EDUCATION

University of Johannesburg, South Africa

June 2011- April 2016

Ph.D. Biochemistry, Thesis: “Calmodulin dependent protein kinase (CaMK) II through exercise regulates Nuclear respiratory factor (NRF)-1: Potential role in Type 2 diabetes and Obesity”. Mahatma Gandhi University, India

August 2002- April 2005

Msc. Biochemistry

Mahatma Gandhi University, India

July 1999- May 2002

Bsc. Chemistry

EXPERIENCE

Hypertension in Africa Research Team, Department of Physiology, University of North West, Potchefstroom Campus, South Africa

June 2017-Current

Postdoctoral Research Fellow

Awarded a Postdoctoral Fellowship from the University of North West, South Africa

Leader of EndoAfrica project team, Title: “Vascular endothelial dysfunction: The putative interface of emerging cardiovascular risk factors affecting populations living with and without HIV in Sub-Saharan Africa”

In charge of lab for EndoAfrica Project

Data collection, capturing and analysis using RedCaP for EndoAfrica Project.

Assisting Masters students in their research

Lecturing Bachelors students

Writing research articles on PURE study done by HART Pharma Q Pvt Ltd, Johannesburg, South Africa January 2016-May 2017 Research and Development Scientist

Conduct research concerning drugs

Performing assays for pharmaceutical products based on new and existing technologies

Operating and training analytical instruments such as HPLC, GC-FID, Atomic absorption spectroscopy, FTIR, Potentiometer, karl-fischer Titration, Refractometer, Viscometer, UV spectroscopy etc.

Set up new and existing methods to identify compounds in pharmaceutical products.

Regular checking of analytical instruments

Training new chemists on new techniques and equipment’s University of Johannesburg, South Africa August 2011- December 2015 Researcher (PhD in Biochemistry)

Skills

Animal model study: Handling, exercising and dissection of muscles of Wistar rats

Cell culture: Growing, differentiating and treating the cells (3T3L1 adipocytes, C2C12 monocytes)

Isolation RNA and expression by Quantitative real time PCR(qPCR)

Extraction of DNA and quantification by gel electrophoresis

Isolation of proteins and expression by Western blotting

Isolation and quantification of lipids (fatty acids) by GC-MS

Identification of cholesterol and TG by TLC

Mitochondria and lipid droplet size and number determination by Transmission electron microscopy (TEM)

Chromatin immuno-precipitation assay for to determine the protein-DNA binding

Flow-cytometry for protein expression

ATP synthesis assessed by Cell Titer Glo assay

Supervised Masters and Honors students in their research project.

Lecture 2nd year Bachelors students

Reivilo High School January 2005-May 2011

Grade 12 Educator

Taught chemistry, Physics and mathematics

Coordinator of e-learning

Member of school management committee

Disciplinary committee in charge

Moderator of Physical science and Biology

Other skills

GraphPad Prism 7 Software

SPSS

Statstica

Microsoft office

Code B driving license

Strengths

Strong communication/presentation skills: presented data clearly and confidently to both small and large groups, at home or abroad.

Collaborated and communicated at all professional levels, and with people from diverse origins and cultures. Can work both independently and in team settings.

Planned work to achieve goals and targets on time, set realistic objectives, developed creative solutions to problems.

Supervised and taught Bachelors, Honors and M.Sc. students, adapting to different scientific levels and backgrounds.

Courses sttended

Senior Tutor training (2013, 2014 and 2015)- University of Johannesburg

Flow cytometry and cell sorting (5-7 March 2012, University of Johannesburg)

NGS data analysis and Galaxy workshop (14-18 October 2013, University of Pretoria) Published peer-reviewed articles

1. Henok H. Knife, Yonas H. Belay, Jitcy S. Joseph, Emmanuel Mukwevho. Evaluation of the influence of thiosemicarbazone– triazole hybrids on genes implicated in lipid oxidation and accumulation as potential anti-obesity agents. Bioorganic & Medicinal Chemistry Letters 23 (2013), 5275–5278.

2. Jitcy S Joseph and Emmanuel Mukwevho. Exercise induced CaMKII activation regulate omega 3 fatty acids in rat skeletal muscle. Chemical Biology Letters (2014), 1(1), 1-10. 3. Jitcy S Joseph and Emmanuel Mukwevho. Calmodulin dependent protein kinase II activation by exercise regulates saturated & unsaturated fatty acids: Improves some metabolic syndrome markers. Life Science (2014), 111(1-2), 53-61. 4. Ayeleso Ademola, Jitcy S Joseph, Belay Yonas, Kinfe Henok, Mazibuko Sithandiwe and Mukwevho Emmanuel. Hybrid compounds from thiosemicarbazone and triazole as antidiabetic agents and their antioxidant potentials. Biomedical Research (2016), 2017, 28, 1

5. Jitcy S joseph, Ayeleso Ademola and Emmanuel Mukwevho. Exercise increases hyperacetylation of histones on the Cis-element of NRF_1 binding to the Mef2a promotor: Implications on type 2 diabetes. Biohemical and Biophysical Research communications (2017) 486(1):83-87.

6. Jitcy S joseph, Ayeleso Ademola and Emmanuel Mukwevho. Role of exercise-induced calmodulin protein kinase (CAMK)II activation in the regulation of omega-6 fatty acids and lipid metabolism genes in rat skeletal muscle. Physiological Research (Sept 22, 2017) (in press).

Conference presentations

1. Jitcy S Joseph and Emmanuel Mukwevho. CaMKII activation during exercise regulates saturated and unsaturated fatty acids in rat skeletal muscle. The 11th Biennial

“International society for the study of fatty acids and lipids” Congress 2014 June 2014, Stockholm, Sweden.

2. Jitcy S Joseph and Emmanuel mukwevho. Exercise induced CaMKII activation increase glucose transport in rat skeletal muscle. Physiological society of Southern Africa. September 2013, Pretoria, South Africa.

3. Emmanuel Mukwevho, Zane Ferreira and Jitcy Joseph. Evaluation of the influence of thiosemicarbazone-triazole hybrids on genes implicated in lipid oxidation and accumulation as potential anti-obesity agents. The 11th Biennial “International society for the study of fatty acids and lipids” Congress 2014.June 2014, Stockholm References

Prof E. Mukwevho, PhD

Professor & Head of Department

Biological Sciences

North West University

Mmabatho 2735

South Africa

Tel no: 018-***-****

Email: ac2t79@r.postjobfree.com

Prof Henok Kinfe, PhD

Associate Professor

Department of Chemistry

University of Johannesburg

Auckland Park

South Africa

Tel Phone: 002*-**-***-****

Email: ac2t79@r.postjobfree.com

Prof Marianne Cronje, PhD

Associate Professor

Department of Biochemistry

University of Johannesburg

Auckland Park

South Africa

Tel Phone: 002*-**-***-****

Email: ac2t79@r.postjobfree.com

Dr Gerrit Koorsen, PhD

Senior Lecturer

Department of Biochemistry

University of Johannesburg

Auckland Park

South Africa

Tel Phone: 002*-**-***-****

Email: ac2t79@r.postjobfree.com

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