CURRICULAM VITAE

*. Name DR. PRAVAS RANJAN KOLE

2. Father’s name NIRANJAN KOLE

3. Mother’s Name SUNANDA KOLE

4. Nationality INDIAN

5. Date of Birth 24-01-1979

6. Gender MALE

7. Religion HINDU

8. Community /Category GENERAL

9. Marital Status Married

10. Permanent Address

C/o Niranajna Kole, 369-Viva Villa Indirapali,

Arambagh, Hooghly, West Bengal-712601.

11. Present Address for

correspondence

Protection of Plant Varieties and Farmer s Right Act

(PPVFRA), NASC Complex, DPS Marg, Opp.

Todapur Village, New Delhi-110012.

Email: ac2rw1@r.postjobfree.com

Mobile: +918*********

12. EDUCATIONAL QUALIFICATION

Examination Name of the Board/ University Year Subject studies Secondary

West Bengal Board of

Secondary Education (WBBSE),

India.

1994

Language Group, Science

Group, India & Her People

Group, Work Education Group.

Intermediate

West Bengal Council of Higher

Secondary Education

(WBCHSE), India.

1996 Language, Biology, Chemistry,

Physics.

Bachelor Degree

The University of Burdwan,

India.

1999 Botany, Zoology, Chemistry.

Master s Degree

Allahabad Agricultural Institute-

Deemed University, Allahabad,

India.

2004

Biotechnology

Ph. D

Bundelkhand University, Jhansi,

India.

2010 Biotechnology

Post Doc

RMIT University, Melbourne,

Australia

2016 Functional Genomics

Ph. D degree details Date of Registration Date of Submission Date of Notification 31-01-2006 18-06-2009 22-04-2010

13. EXPERIENCE

Designation Name and address of the

employer

Period of experience Major discipline of work

experience

Date

from

Date To

Technical

Examiner

Protection of Plant Varieties

and Farmer s Right Act

(PPVFRA), NASC Complex,

DPS Marg, New Delhi-

110012.

03-04-

2017

Till date

Examination of

application for new

varieties registration and

Distinctiveness,

Uniformity and Stability

(DUS) test result analysis

for registration process.

Independent

Researcher

Dr. K. V. Bhat, Pr. Scientist,

Division of Genomic

Resources, NBPGR, Pusa

Campus, New Delhi, India.

04-08-

2016

31-03-

2017

Functional Genomics.

High Throughput

Sequencing using Illumina

MiSeq, Ion Torrent.

Guidance to Ph D

Scholars.

Visiting

Researcher

School of Applied Sciences,

Health Innovations Research

Institute, RMIT University,

Plenty Road, Melbourne,

Vitoria, Australia.

23-07-

2014

23-07-

2016

Functional Genomics in

Chickpea. High

Throughput Sequencing

using Illumina HiSeq, Ion

Proton. Gene expression

using RT-PCR.

Research

Associate

Dr. K. V. Bhat, Pr. Scientist,

Division of Genomic

Resources, NBPGR, Pusa

Campus, New Delhi, India.

17-07-

2013

30-06-

2014

Molecular Biology, Plant

Biosystemetics study in

Abelmoschus (Bhendi),

Cucumis and Vigna sp.

Technical

Assistant

DNA Fingerprinting Lab,

QCL(N National Seed

Corporation Ltd, Pusa

Campus, New Delhi, India.

02-01-

2012

01-07-

2013

Molecular Biology,

Genetic Purity and hybrid

testing,

Senior

Research

Fellow

Dr. K. V. Bhat, Pr. Scientist,

Division of Genomic

Resources, NBPGR, Pusa

Campus, New Delhi, India.

13-12-

2007

31-12-

2011

Molecular Biology, DNA

fingerprinting, molecular

marker analysis.

Research

Trainee

Prof. C. Kole, Allahabad

Agriculture Institute,

Allahabad. Uttar Pradesh.

01-12-

2004

31-08-

2005

Molecular Marker

analysis and Genome

Mapping

Nature of works/ Duties

Independent Researcher

Functional Genomics. High Throughput Sequencing using Illumina MiSeq, Ion Torrent.

Sample QC, library preparation, library QC monitoring, sequencing, data QC and operate NGS machines (Illumina MiSeq and Ion Torrent) in the lab.

Guidance to Ph D Scholars.

Visiting Researcher

Gene expression studies of phytohormone signaling genes using RT-PCR.

Hands on experience in RNA Sequencing including small RNA, microRNA, long noncoding RNA using Illumina MySeq and HiSeq 3000 platform.

Sample QC, library preparation, library QC monitoring, sequencing, data QC and operate NGS machines (Illumina MiSeq and HiSeq 2500) in the lab.

Hands on experience in whole genome DNA methylation study using HiSeq 3000 platform.

Bioinformatics tools for RNA-seq and DNA methylation analysis.

Participated in teaching to graduate and post graduate students on Functional Genomics & Proteomics (BIOL 2267).

Research Associate

Planning and scheduling: departmental and team activities.

DNA fingerprinting of Vigna, Cucumus and Abelmoschus

Planting of mapping population, maintenance and seed production in the glass house and the experimental field.

Guiding post graduate and PhD students to carry out their proposed research work

Maintenance of the equipments and purchasing consumables for the lab. Technical Assistant

Establishment of DNA Finger Printing laboratory for genetic purity check of different varieties of seed at National Seed Corporation Ltd.

Planning and scheduling of departmental and team activities.

Overall maintenance of the DNA Finger Printing Laboratory

Maintenance of the equipments

Indenting and arranging the required chemicals for the DNA fingerprinting analysis

Imparting training to graduate and post-graduate students

Regular audit faced for internal and external audit Senior Research Fellow

Collection and storage of samples received from different states of India.

DNA fingerprinting and molecular characterization of Jatropha, Pongamia

Five hundred collected Jatropha accessions and seventy Pongamia accessions are being fingerprinted using RAPD, SSR, SRAP and AFLP markers

Data maintain

Helped post-graduates students to carry out their project work.

Over all responsible for the lab as I was the senior most from 2007 to 2011.

Stock register maintain for chemicals and plastic ware. Research Trainee

Development of recombinant inbreed population for genome mapping

Data collection for qualitative and quantitative characters of economic importance

Marker analysis (SSR, AFLP and RAPD) to detect parental polymorphism and genotyping the mapping population.

14. LEADERSHIP

Established DNA Finger Printing laboratory (2012) for genetic purity check of different varieties of seeds at National Seed Corporation Ltd., New Delhi.

Overall responsibility of the molecular biology lab (2007-2011) at Division of Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi. 15. RESEARCH PROJECT COMPLETED

Sl

No

Title of the project Sponsoring Agency Host Institute Period Amount in

(lakh)

From To

1 Functional validation

of some key abiotic

stress tolerance genes

of Chickpea (Cicer

arietinum L.) using

RNAi technology

Department of

Biotechnology (DBT),

Government of India

School of Applied

Science, RMIT

University,

Melbourne,

Victoria,

Australia.

2014 2016 37.20

16. RESEARCH PUBLICATIONS

Bhat K V, Mondal T K, Gaikwad A B, Kole P R, Chandel G. (2017) Genome-wide identification of drought-responsive miRNAs in grass pea (Lathyrus sativus L.). Under Review in DNA Research.

Rajappa J J, Kole P R, Umdale S D, Bhat V K (2017). Characterization and comparative analysis of conserved orthologous markers in Vigna germplasm in Indian collections.

(Communicated at Proceedings of the National Academy of Sciences, India Section B: Biological Sciences).

Sharma M K, P R Kole, Sengar R S (2017). In Vitro clonal propagation and genetic fidelity assessment in sugarcane by using RAPD markers. (Communicated at Plant Cell Tissue and Organ Culture)

Kaashyp M, Ford R, Kole P R, Mantri N et al. (2017) RNA-Seq analysis reveals gene(s) expression profile in response to salt stress at vegetative and reproductive stages in chickpea root tissues. (Communicated at Plant Physiology).

Bhaskarla V, Kole P R, Mantri N et al. (2017). Differential expression gene analysis of chickpea using RNA seq under drought stress. (Communicated at Scientific Reports).

Thejazhanuo L M, Changkija S, Pattanayak A, Chaturvedi H P, Devi S V, Kole P R

(2017). Genetic Characterization of Locally Cultivated Taro Germplasm from Eleven District of Nagaland. International Journal of Current Microbiology and Applied Science. 6(8): 3338-3348. doi: https://doi.org/10.20546/ijcmas.2017.608.398

Saxena S, Kole P R, Bhat K V, Pandey S P (2016). Species diversity and relationship among Vigna radiata (L.) Wilczek and its close wild relatives. Bangladesh Journal of Botany. 45(3): 589-595.

Kole P R, Rana R S, Bhat K V (2016). Optimization of the choice of molecular markers for identification of commercially used rice varieties in India using rapid DNA extraction protocol. Journal of Applied and Natural Science. 8(2): 1028-1034.

Rajappa J J, Umdale S D, Kole P R, Bhat V K (2016). Identification of Conserved Orthologous Set Markers in Cultivated Vigna radiata (L.)Wilczek. VEGETOS. 29:3

Kole P R, Sharma M K, Kumar S, Kumar A, Singh S, Sengar R S (2015). Assessment of genetic diversity in Indian cultivated pea (Pisum sativum L.) by using SRAP markers. VEGITOS. 28(3):84-91.

Kole P R, Bhat K V, Chaudhuri R, Malik S K, Rao G R. (2015).Genetic Diversity Analysis in wild species of Pongamia pinnata (L.) using RAPD and SRAP markers. Journal of Advances in Biotechnology. 4(3):442-452.

Kole P R, Bhat K V, Chaudhuri R, Malik S K (2015).Genetic variation among Jatropha Curcas L. using dominant molecular marker collected from different agro-climatic regions of India. Indian Journal of genetics and plant Breeding. 75(2):267-270. Article DOI: 10.5958/0975-6906.2015.00043.7

Kole P R, Sharma M K, Kumar S, Kumar R (2015). Genetic diversity assessment in Indian cultivated pea (Pisum sativum L.) varieties using RAPD markers. Journal of Applied and Natural Science. 7 (1): 316-323.

Kole P R, Bhat K V, Mathew J, Saxena S (2013). Species diversity and relationship among Vigna mungo and its close wild relatives using Sequence Tagged Microsatellite Sites (STMS) marker. African Journal of Biotechnology. 12(4):327-335

Malik S K, Uchoi A, Kumar S, Choudhary R, Pal D, Kole P R, Chaudhury R, Bhat K V

(2013). Molecular characterization of Citrus macroptera Montr. (Satkara): an endangered wild species from Northeastern India”. Plant Biosystems, iFirst article:1–7.

Malik S K, Kumar S, Choudhary R, Kole P R, Chaudhury R, Bhat K V (2013). Genetic diversity in Khirni [Manilkara hexandra (Roxb.) Dubard]: An important underutilized fruit species of India using Random Amplified Polymorphic DNA (RAPD) markers. Indian Journal of Horticulture. 70(1):18-25.

Kole P R, Bhat K V, Chaudhury R (2011). Molecular approaches for variability studies in Jatropha curcas, a biofuel crop. ICAR News (A science and technology newsletter). 17(1):5.

Kole C, Mohanty B, Pal A, Singh B, Kole P R, Shukla N (2006). Electrophoretic Studies on Seed Protein profile of blackgram, Vigna mungo (L.) Hepper. Legume Research. 29

(4): 235-241.

Singh R, Kole P R, Saxena S, Ray A, Shukla N, Kole C (2005) Estimation of leaf area in green gram using linear measurement. Crop Research. 29(3):521-523. 17. RESEARCH PUBLICATIONS COMMUNICATED

Kole P R, Bhaskarla V, Kaashyp M, Ford R et al. (2017) Genome wide DNA methylation analysis in response to abiotic stresses in chickpea.(In preparation).

Ford R, Mantri N, Kole P R et al. (2017) High-throughput sequencing discovery of conserve and novel microRNAs in chickpea (Cicer arietinum L In preparation).

Ford R, Mantri N, Kole P R et al. (2017) Identification and characterization of drought associated smallRNAs in Cicer arietinum L.(In preparation). 18. POSTER PRESENTATION

Kole P R, Shubhanjli, Bhat K V. Genetic Purity of Variety and Hybrids: testing technologies for better seed quality. At International Conference of microbial, plant and animal research (ICMPAR-2012) March 29-31, 2012.

Shubhanjli, Kole P R, Bhat K V. Species Diversity and Relationship among Vigna radiate

(L.) and its close relatives using microsatellite marker. At International Conference of microbial, plant and animal research (ICMPAR-2012) March 29-31, 2012.

Kole P R, Bhat K V, Chudhury R, Josi J. DNA Fingerprinting and molecular characterization of Jatropha germplasm collected from diverse agro-climatic zones of India. At National Symposium on Recent Global Developments in the Management of Plant Genetic Resources on 17-18 December 2009 organized by ISPGR and NBPGR. 19. BOOK CHAPTER

Bhat KV, Kumari R, Kole P R, Mandal N. Sequence Characterized Amplified Regions Analysis. Training manual for Winter School on Molecular Markers for Efficient Management and Enhancing Utilization of Plant Genetic Resources, NBPGR, New Delhi, India

Bhat KV & Kole P R (2013). Molecular markers and status and utility of genomic resources in Jatropha. CSIR manual.

20. TRAINING COURSES

Participate in a training course on “Conservation of Plant Genomics Resources” during 12-25 March, 2012, conducted at National Bureau of Pant Genetic Resources sponsored by National Agricultural Innovation Project (ICAR), New Delhi.

Participated in a training Programme on Forest Biotechnology for ICFRE, Dehradun as a laboratory resource parson, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 11th to 20th July, 2011.

Participated in ICAR conducted Winter School as a laboratory resource person on Molecular Markers for Efficient Management and Enhancing Utilization of Plant Genetic Resources, NBPGR, New Delhi, India. 5th to 16th January 2010.

Participated in international training course as a laboratory resource person on In vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources organized by ICAR, NBPGR, Bioversity International and APAARI, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 15th and 27th November, 2010.

Participated in international training course as a laboratory resource person on In vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources organized by ICAR, NBPGR, Bioversity International and APAARI, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 17th and 29th November, 2008. 21. THESIS/PROJECTS

Ph D Research: “Molecular mapping of genes conferring disease resistance in urdbean Vigna mungo (L.) Hepper using RAPD and AFLP markers”. Organization: Bundelhkand University, Jhansi, Uttar Pradesh. Skills:

Growing mapping population in the experimental field

Development of molecular marker

Polyacrylamide gel electrophoresis (PAGE)

Post-Graduate Project: “Genetic diversity and expression analysis of selected mutants of Catharanthus roseus.”

Organization: Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India.

Skills:

Growing of Mutants and controls in the field

Scoring of morphological characters

Amplified fragment length polymorphism (AFLP) on manual sequencer ABI Prism

Transformation and cloning

22. COMPUTER PROFICIENCY

Operating Systems: Windows2000, XP, Vista, Windows7 and Mac OS X

Packages: Microsoft Office Tools & Statistic

Bioinformatics tools: TopHat, Cufflinks, MethylKit, Numerical Taxonomy Software

(NTSYS PC Ver. 2.02).

DECLARATION

I hereby declare that all the statements made above are true, complete and correct to the best of my knowledge and belief.

Place: New Delhi.

Dr. Pravas Ranjan Kole

REFERENCES

Dr. Tapan Kumar Mondal

Senior Scientist (Plant Biotechnology),

ICAR-National Research Center on Plant Biotechnology, Pusa Campus,

New Delhi -110012. India.

Phone: +91-995*******

ac2rw1@r.postjobfree.com

Dr. Nitin Mantri

Senior Lecturer in Biotechnology

Health Innovations Research Institute

School of Science

RMIT University

Building 223, Level 1

Plenty Road, Bundoora.

Victoria. 3083. Australia.

Email: ac2rw1@r.postjobfree.com

Off.: +61 3 9925 7152

Dr. K. V. Bhat

Principal Scientist

Division of Genomic Resources,

National Bureau of Plant Genetic Resources

New Delhi-12

Email: ac2rw1@r.postjobfree.com

+91-981*******.

Contact this candidate