Plant Resources
Resume:
CURRICULAM VITAE
*. Name DR. PRAVAS RANJAN KOLE
2. Father’s name NIRANJAN KOLE
3. Mother’s Name SUNANDA KOLE
4. Nationality INDIAN
5. Date of Birth 24-01-1979
6. Gender MALE
7. Religion HINDU
8. Community /Category GENERAL
9. Marital Status Married
10. Permanent Address
C/o Niranajna Kole, 369-Viva Villa Indirapali,
Arambagh, Hooghly, West Bengal-712601.
11. Present Address for
correspondence
Protection of Plant Varieties and Farmer s Right Act
(PPVFRA), NASC Complex, DPS Marg, Opp.
Todapur Village, New Delhi-110012.
Email: **********@*****.***
Mobile: +918*********
12. EDUCATIONAL QUALIFICATION
Examination Name of the Board/ University Year Subject studies Secondary
West Bengal Board of
Secondary Education (WBBSE),
India.
1994
Language Group, Science
Group, India & Her People
Group, Work Education Group.
Intermediate
West Bengal Council of Higher
Secondary Education
(WBCHSE), India.
1996 Language, Biology, Chemistry,
Physics.
Bachelor Degree
The University of Burdwan,
India.
1999 Botany, Zoology, Chemistry.
Master s Degree
Allahabad Agricultural Institute-
Deemed University, Allahabad,
India.
2004
Biotechnology
Ph. D
Bundelkhand University, Jhansi,
India.
2010 Biotechnology
Post Doc
RMIT University, Melbourne,
Australia
2016 Functional Genomics
Ph. D degree details Date of Registration Date of Submission Date of Notification 31-01-2006 18-06-2009 22-04-2010
13. EXPERIENCE
Designation Name and address of the
employer
Period of experience Major discipline of work
experience
Date
from
Date To
Technical
Examiner
Protection of Plant Varieties
and Farmer s Right Act
(PPVFRA), NASC Complex,
DPS Marg, New Delhi-
110012.
03-04-
2017
Till date
Examination of
application for new
varieties registration and
Distinctiveness,
Uniformity and Stability
(DUS) test result analysis
for registration process.
Independent
Researcher
Dr. K. V. Bhat, Pr. Scientist,
Division of Genomic
Resources, NBPGR, Pusa
Campus, New Delhi, India.
04-08-
2016
31-03-
2017
Functional Genomics.
High Throughput
Sequencing using Illumina
MiSeq, Ion Torrent.
Guidance to Ph D
Scholars.
Visiting
Researcher
School of Applied Sciences,
Health Innovations Research
Institute, RMIT University,
Plenty Road, Melbourne,
Vitoria, Australia.
23-07-
2014
23-07-
2016
Functional Genomics in
Chickpea. High
Throughput Sequencing
using Illumina HiSeq, Ion
Proton. Gene expression
using RT-PCR.
Research
Associate
Dr. K. V. Bhat, Pr. Scientist,
Division of Genomic
Resources, NBPGR, Pusa
Campus, New Delhi, India.
17-07-
2013
30-06-
2014
Molecular Biology, Plant
Biosystemetics study in
Abelmoschus (Bhendi),
Cucumis and Vigna sp.
Technical
Assistant
DNA Fingerprinting Lab,
QCL(N National Seed
Corporation Ltd, Pusa
Campus, New Delhi, India.
02-01-
2012
01-07-
2013
Molecular Biology,
Genetic Purity and hybrid
testing,
Senior
Research
Fellow
Dr. K. V. Bhat, Pr. Scientist,
Division of Genomic
Resources, NBPGR, Pusa
Campus, New Delhi, India.
13-12-
2007
31-12-
2011
Molecular Biology, DNA
fingerprinting, molecular
marker analysis.
Research
Trainee
Prof. C. Kole, Allahabad
Agriculture Institute,
Allahabad. Uttar Pradesh.
01-12-
2004
31-08-
2005
Molecular Marker
analysis and Genome
Mapping
Nature of works/ Duties
Independent Researcher
Functional Genomics. High Throughput Sequencing using Illumina MiSeq, Ion Torrent.
Sample QC, library preparation, library QC monitoring, sequencing, data QC and operate NGS machines (Illumina MiSeq and Ion Torrent) in the lab.
Guidance to Ph D Scholars.
Visiting Researcher
Gene expression studies of phytohormone signaling genes using RT-PCR.
Hands on experience in RNA Sequencing including small RNA, microRNA, long noncoding RNA using Illumina MySeq and HiSeq 3000 platform.
Sample QC, library preparation, library QC monitoring, sequencing, data QC and operate NGS machines (Illumina MiSeq and HiSeq 2500) in the lab.
Hands on experience in whole genome DNA methylation study using HiSeq 3000 platform.
Bioinformatics tools for RNA-seq and DNA methylation analysis.
Participated in teaching to graduate and post graduate students on Functional Genomics & Proteomics (BIOL 2267).
Research Associate
Planning and scheduling: departmental and team activities.
DNA fingerprinting of Vigna, Cucumus and Abelmoschus
Planting of mapping population, maintenance and seed production in the glass house and the experimental field.
Guiding post graduate and PhD students to carry out their proposed research work
Maintenance of the equipments and purchasing consumables for the lab. Technical Assistant
Establishment of DNA Finger Printing laboratory for genetic purity check of different varieties of seed at National Seed Corporation Ltd.
Planning and scheduling of departmental and team activities.
Overall maintenance of the DNA Finger Printing Laboratory
Maintenance of the equipments
Indenting and arranging the required chemicals for the DNA fingerprinting analysis
Imparting training to graduate and post-graduate students
Regular audit faced for internal and external audit Senior Research Fellow
Collection and storage of samples received from different states of India.
DNA fingerprinting and molecular characterization of Jatropha, Pongamia
Five hundred collected Jatropha accessions and seventy Pongamia accessions are being fingerprinted using RAPD, SSR, SRAP and AFLP markers
Data maintain
Helped post-graduates students to carry out their project work.
Over all responsible for the lab as I was the senior most from 2007 to 2011.
Stock register maintain for chemicals and plastic ware. Research Trainee
Development of recombinant inbreed population for genome mapping
Data collection for qualitative and quantitative characters of economic importance
Marker analysis (SSR, AFLP and RAPD) to detect parental polymorphism and genotyping the mapping population.
14. LEADERSHIP
Established DNA Finger Printing laboratory (2012) for genetic purity check of different varieties of seeds at National Seed Corporation Ltd., New Delhi.
Overall responsibility of the molecular biology lab (2007-2011) at Division of Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi. 15. RESEARCH PROJECT COMPLETED
Sl
No
Title of the project Sponsoring Agency Host Institute Period Amount in
(lakh)
From To
1 Functional validation
of some key abiotic
stress tolerance genes
of Chickpea (Cicer
arietinum L.) using
RNAi technology
Department of
Biotechnology (DBT),
Government of India
School of Applied
Science, RMIT
University,
Melbourne,
Victoria,
Australia.
2014 2016 37.20
16. RESEARCH PUBLICATIONS
Bhat K V, Mondal T K, Gaikwad A B, Kole P R, Chandel G. (2017) Genome-wide identification of drought-responsive miRNAs in grass pea (Lathyrus sativus L.). Under Review in DNA Research.
Rajappa J J, Kole P R, Umdale S D, Bhat V K (2017). Characterization and comparative analysis of conserved orthologous markers in Vigna germplasm in Indian collections.
(Communicated at Proceedings of the National Academy of Sciences, India Section B: Biological Sciences).
Sharma M K, P R Kole, Sengar R S (2017). In Vitro clonal propagation and genetic fidelity assessment in sugarcane by using RAPD markers. (Communicated at Plant Cell Tissue and Organ Culture)
Kaashyp M, Ford R, Kole P R, Mantri N et al. (2017) RNA-Seq analysis reveals gene(s) expression profile in response to salt stress at vegetative and reproductive stages in chickpea root tissues. (Communicated at Plant Physiology).
Bhaskarla V, Kole P R, Mantri N et al. (2017). Differential expression gene analysis of chickpea using RNA seq under drought stress. (Communicated at Scientific Reports).
Thejazhanuo L M, Changkija S, Pattanayak A, Chaturvedi H P, Devi S V, Kole P R
(2017). Genetic Characterization of Locally Cultivated Taro Germplasm from Eleven District of Nagaland. International Journal of Current Microbiology and Applied Science. 6(8): 3338-3348. doi: https://doi.org/10.20546/ijcmas.2017.608.398
Saxena S, Kole P R, Bhat K V, Pandey S P (2016). Species diversity and relationship among Vigna radiata (L.) Wilczek and its close wild relatives. Bangladesh Journal of Botany. 45(3): 589-595.
Kole P R, Rana R S, Bhat K V (2016). Optimization of the choice of molecular markers for identification of commercially used rice varieties in India using rapid DNA extraction protocol. Journal of Applied and Natural Science. 8(2): 1028-1034.
Rajappa J J, Umdale S D, Kole P R, Bhat V K (2016). Identification of Conserved Orthologous Set Markers in Cultivated Vigna radiata (L.)Wilczek. VEGETOS. 29:3
Kole P R, Sharma M K, Kumar S, Kumar A, Singh S, Sengar R S (2015). Assessment of genetic diversity in Indian cultivated pea (Pisum sativum L.) by using SRAP markers. VEGITOS. 28(3):84-91.
Kole P R, Bhat K V, Chaudhuri R, Malik S K, Rao G R. (2015).Genetic Diversity Analysis in wild species of Pongamia pinnata (L.) using RAPD and SRAP markers. Journal of Advances in Biotechnology. 4(3):442-452.
Kole P R, Bhat K V, Chaudhuri R, Malik S K (2015).Genetic variation among Jatropha Curcas L. using dominant molecular marker collected from different agro-climatic regions of India. Indian Journal of genetics and plant Breeding. 75(2):267-270. Article DOI: 10.5958/0975-6906.2015.00043.7
Kole P R, Sharma M K, Kumar S, Kumar R (2015). Genetic diversity assessment in Indian cultivated pea (Pisum sativum L.) varieties using RAPD markers. Journal of Applied and Natural Science. 7 (1): 316-323.
Kole P R, Bhat K V, Mathew J, Saxena S (2013). Species diversity and relationship among Vigna mungo and its close wild relatives using Sequence Tagged Microsatellite Sites (STMS) marker. African Journal of Biotechnology. 12(4):327-335
Malik S K, Uchoi A, Kumar S, Choudhary R, Pal D, Kole P R, Chaudhury R, Bhat K V
(2013). Molecular characterization of Citrus macroptera Montr. (Satkara): an endangered wild species from Northeastern India”. Plant Biosystems, iFirst article:1–7.
Malik S K, Kumar S, Choudhary R, Kole P R, Chaudhury R, Bhat K V (2013). Genetic diversity in Khirni [Manilkara hexandra (Roxb.) Dubard]: An important underutilized fruit species of India using Random Amplified Polymorphic DNA (RAPD) markers. Indian Journal of Horticulture. 70(1):18-25.
Kole P R, Bhat K V, Chaudhury R (2011). Molecular approaches for variability studies in Jatropha curcas, a biofuel crop. ICAR News (A science and technology newsletter). 17(1):5.
Kole C, Mohanty B, Pal A, Singh B, Kole P R, Shukla N (2006). Electrophoretic Studies on Seed Protein profile of blackgram, Vigna mungo (L.) Hepper. Legume Research. 29
(4): 235-241.
Singh R, Kole P R, Saxena S, Ray A, Shukla N, Kole C (2005) Estimation of leaf area in green gram using linear measurement. Crop Research. 29(3):521-523. 17. RESEARCH PUBLICATIONS COMMUNICATED
Kole P R, Bhaskarla V, Kaashyp M, Ford R et al. (2017) Genome wide DNA methylation analysis in response to abiotic stresses in chickpea.(In preparation).
Ford R, Mantri N, Kole P R et al. (2017) High-throughput sequencing discovery of conserve and novel microRNAs in chickpea (Cicer arietinum L In preparation).
Ford R, Mantri N, Kole P R et al. (2017) Identification and characterization of drought associated smallRNAs in Cicer arietinum L.(In preparation). 18. POSTER PRESENTATION
Kole P R, Shubhanjli, Bhat K V. Genetic Purity of Variety and Hybrids: testing technologies for better seed quality. At International Conference of microbial, plant and animal research (ICMPAR-2012) March 29-31, 2012.
Shubhanjli, Kole P R, Bhat K V. Species Diversity and Relationship among Vigna radiate
(L.) and its close relatives using microsatellite marker. At International Conference of microbial, plant and animal research (ICMPAR-2012) March 29-31, 2012.
Kole P R, Bhat K V, Chudhury R, Josi J. DNA Fingerprinting and molecular characterization of Jatropha germplasm collected from diverse agro-climatic zones of India. At National Symposium on Recent Global Developments in the Management of Plant Genetic Resources on 17-18 December 2009 organized by ISPGR and NBPGR. 19. BOOK CHAPTER
Bhat KV, Kumari R, Kole P R, Mandal N. Sequence Characterized Amplified Regions Analysis. Training manual for Winter School on Molecular Markers for Efficient Management and Enhancing Utilization of Plant Genetic Resources, NBPGR, New Delhi, India
Bhat KV & Kole P R (2013). Molecular markers and status and utility of genomic resources in Jatropha. CSIR manual.
20. TRAINING COURSES
Participate in a training course on “Conservation of Plant Genomics Resources” during 12-25 March, 2012, conducted at National Bureau of Pant Genetic Resources sponsored by National Agricultural Innovation Project (ICAR), New Delhi.
Participated in a training Programme on Forest Biotechnology for ICFRE, Dehradun as a laboratory resource parson, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 11th to 20th July, 2011.
Participated in ICAR conducted Winter School as a laboratory resource person on Molecular Markers for Efficient Management and Enhancing Utilization of Plant Genetic Resources, NBPGR, New Delhi, India. 5th to 16th January 2010.
Participated in international training course as a laboratory resource person on In vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources organized by ICAR, NBPGR, Bioversity International and APAARI, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 15th and 27th November, 2010.
Participated in international training course as a laboratory resource person on In vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources organized by ICAR, NBPGR, Bioversity International and APAARI, conducted at the National Bureau of Plant Genetic Resources, New Delhi on 17th and 29th November, 2008. 21. THESIS/PROJECTS
Ph D Research: “Molecular mapping of genes conferring disease resistance in urdbean Vigna mungo (L.) Hepper using RAPD and AFLP markers”. Organization: Bundelhkand University, Jhansi, Uttar Pradesh. Skills:
Growing mapping population in the experimental field
Development of molecular marker
Polyacrylamide gel electrophoresis (PAGE)
Post-Graduate Project: “Genetic diversity and expression analysis of selected mutants of Catharanthus roseus.”
Organization: Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India.
Skills:
Growing of Mutants and controls in the field
Scoring of morphological characters
Amplified fragment length polymorphism (AFLP) on manual sequencer ABI Prism
Transformation and cloning
22. COMPUTER PROFICIENCY
Operating Systems: Windows2000, XP, Vista, Windows7 and Mac OS X
Packages: Microsoft Office Tools & Statistic
Bioinformatics tools: TopHat, Cufflinks, MethylKit, Numerical Taxonomy Software
(NTSYS PC Ver. 2.02).
DECLARATION
I hereby declare that all the statements made above are true, complete and correct to the best of my knowledge and belief.
Place: New Delhi.
Dr. Pravas Ranjan Kole
REFERENCES
Dr. Tapan Kumar Mondal
Senior Scientist (Plant Biotechnology),
ICAR-National Research Center on Plant Biotechnology, Pusa Campus,
New Delhi -110012. India.
Phone: +91-995*******
********@**********.***
Dr. Nitin Mantri
Senior Lecturer in Biotechnology
Health Innovations Research Institute
School of Science
RMIT University
Building 223, Level 1
Plenty Road, Bundoora.
Victoria. 3083. Australia.
Email: *****.******@****.***.**
Off.: +61 3 9925 7152
Dr. K. V. Bhat
Principal Scientist
Division of Genomic Resources,
National Bureau of Plant Genetic Resources
New Delhi-12
Email: **********@*****.***
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