Qing Zhou

*** ****** ******* ****, ****** Hill, NC 27514•Phone: 919-***-****•Email: ac2pvr@r.postjobfree.com

Biochemist/R & D Specialist/Associate Scientist

In Crop Science Lab /Biochemistry Lab/Research Lab

Qualifications Summary

An enthusiastic Biochemist based in RTP, NC, with 26 years of experience in an analytical lab, including ELISA, Western Blot, HPLC, AA, wet chemistry, microbiological tests and LIMS; current experience with protein extraction and ELISA analysis, quantify the protein expression levels in various plant tissues of transgenic plants in high-throughput lab such as Bayer Crop science and BASF; experience of lab automation; experience in ISO 17025, GLP and GMP labs to support food safety, the deregulation or approval of transgenic plants and testing of pharmaceutical drug products; ELISA method development and method validation; some experience in documentation and technical reports. Excellent records of consistent and accurate test results. Excellent attention to detail, organizational and record-keeping.

Career Objective

Looking a challenging and responsible research and analytical positions where my expertise in Quantitative ELISA, HPLC, AA, wet chemistry and other analytical and bioanalytical techniques will be utilized in Crop science, Pharmaceutical industries or environmental laboratory.

Education

Peking University, Beijing, China (10/03/1978–07/06/1982)

B.S., June 1982, Environmental Chemistry (Analytical Chemistry / Environmental Science)

Techniques and skills:

Transgenic Pant analysis(GMO): protein quantification and characterization. Sample receiving, lyophilization, extraction, analysis, stability study, method development and validation. Quantify the protein expression levels in various tissues using ELISA. Perform GLP experiments to support Study Directors and Principle Investigators in the conduct of regulatory protein safety studies, which support the approval of transgenic plants. Assist in the preparation of the GLP final reports, including method validation.

Sample preparation & extractions for various samples and matrixes: protein, DNA, pesticide residue (SPE cleanups, Derivatization & purification for HPLC, GC and LC-MS analysis), trace metal and metal (sample digestion for FLAA/GFAA and ICP analysis), inorganic samples.

Molecular biology analysis in Crop Science industry: ligand binding assays and quantitative immunoassays (ELISA, HTRF, Western Blot, SDS-PAGE) for transgenic plants (GMO detection) including protein/DNA extractions from various plant tissues, high throughput and lab automation, protein method development / method validation, and method writing/revising. Protein analysis (BCA method). Protein sample type: food and feed; plant tissue type: immature seed, mature seed, leaf, boll, root, pollen, meal, RBD Oil, Crude Oil and plant-produced Protein.

Agricultural lab: analysis of food (including protein samples), feed, forage and fertilizer for compliance purpose using HPLC, GC, IC, ELISA, and other techniques, including Pesticides Residue, Food Allergen and Mycotoxin.

GLP/GMP lab experience.

ISO 17025 lab (State/Federal FDA regulatory compliance): sample analysis, lab personal training, corrective action, preventive action, proficiency testing, data review, QA/QC, method writing/revising to meet ISO 17025 standards

Notes: ISO 17025 Regulations has similar requirements to compare with GLP on equipment’s, sampling, validation, reporting/records and control of records, audits, corrective actions, QC control procedures.

Environmental chemistry lab (State/ Federal EPA regulatory compliance): Conducted treatability tests on water/wastewater and industrial wastewater using quantitative chemical analyses methods, including pH, DO, conductivity, Acidity (titration), Alkalinity, sulfate, TSS, TS, TDS, BOD, COD, TOC, Chloride, Fluoride, Nitrate, Nitrite, Ammonia, TKN, total P, turbidity, Fecal and Total coliforms.

Instrumentation: INTEGRA VIOFLO-96 Electronic Pipette, E1-ClipTip pipette (auto multiple channel pipette), Bioteck EL406 Washer Dispenser with Bioteck Stacker (Semi-Automated ELISA Liquid Handling with EL406), Epoch 2 Microplate Spectrophotometer with Gen5 Software, Microplate Microplate reader (SpectraMax M4, Tecan Infinte M1000 and M200), Microplate washer (BioTek ELx405), automate DNA purification system (KingFisher Flex system), automated liquid handler (Tecan Freedom Evo work station, LGC Meridian liquid dispenser), lyophilizers/freeze

Qing Zhou

dryers (Millrock Lyophilizer), tissue homogenizer (2010 Geno/Grinder®), Western Blot systems (BIO RAD “Trans-Blot SD Semi-Dry electrophoretic Transfer Cell), HPLC instrument (Agilent HPLC 1100/1260, detectors: UV/VIS: diode- array, Fluorescence, and Refractive Index),, FLAA/GFAA analyzer (Perkin-Elmer AAnalyst 800, Post column derivatizer (Pickering Laboratories PCX 5200 Two-Reagent Post-Column Derivatizer),

N-Evaporator and Rotary-Evaporator for HPLC analysis, Milk analyzers (FOSS MilkoScan Minor, CEM SmartTrac analyzer), metal digestors (CEM MARS 6 Microwave, HotBlock digestor), COD analyzer, TOC analyzer, Segmented Flow Analyzer, pH, D.O., Turbidity, and Conductivity meters, Precision Systems Cryoscope, autoclave.

Computer software: proficiency of MS Office software (Excel, Word, PowerPointb & Outlook); Sharepoint, LIMS (StarLIMS, LabworksLIMS).

Work Experience

Scientific Technician, contractor through CTG, 7/31/2017 to 10/3/2017

Bayer CropScience, Innovation Center, Ag Biotech Research and Development, Trait Validation - Plant Analytics

3500 Paramount Pkwy, Morrisville, NC 27560 919-***-****

The primary responsibility is: independently analyze transgenic plant samples by ELISA to support ongoing research as well as product development.

Perform detailed analysis of transgenic plants using ELISA, BCA and Bradford protein analysis techniques;

• Prepare adequate reports and inform the research project team(s) on a regular basis;

• The characterization of transgenic plants by molecular and biochemical methods;

• Coordinate field trial studies to characterize transgenic herbicide- and insect-resistant corn and soy events;

• Manage confidentiality of competitive know-how;

• Ensure compliance with all existing safety and stewardship standards in the area of responsibility

Scientist - Regulatory Protein Biochemist: contractor through Hunter International, Inc., 1/4/2017 to 7/28/2017

BASF Plant Science, Regulatory Protein Analytics, Regulatory Sciences laboratory

26 Davis Dr., Morrisville, NC 27560

BASF: website http://www.basf.com, 919-***-****

Tasks Include: Perform Good Laboratory Practices (GLP) laboratory experiments to support Study Directors and Principal Investigators in the conduct of regulatory protein safety studies; assist in the preparation of the GLP Final Reports.

Responsibilities of the Scientist – Immunology:

Conduct protein extraction in transgenic plant for ELISA analysis

Characterize expressed novel proteins & quantify the protein expression levels in various tissues or food/feed matrices

Characterize the protein test substances used in a variety of studies

Determine the stability of proteins in the tissues

Immunoassay validation and development

Catalent Pharma Solutions, Department of Inhalation, 160 Pharma Drive, Morrisville, NC 27560

Web: http://www.catalent.com

Sr. Associate Scientist – contractor through Lab Support, 7/25/2016 to 11/01/2016

Responsibilities include: to perform analysis efficiently, as required in line with contracts approved by clients or internal Catalent., to assure the integrity and quality of analytical data produced by adhering to all relevant in-house and client

Qing Zhou

Work Experience (continue)

SOPs and methodologies and following the requirements of GXP (GLP/GMP), to support testing of pharmaceutical drug products.

Test methods to be executed include dose content uniformity, cascade impaction by Andersen Cascade Impactor, etc. To conduct drug assay analysis using RP-HPLC. Method validation: Specificity, Linearity, Precision, Accuracy, Detection and Quantitation Limit (LOD and LOQ), Robustness.

Bayer Crop Science, Breeding & Trait Development, Crop Analytics. Tech III 407 Davis Drive, Morrisville, NC 27560

Web: http://www.bayercropscience.com

R & D Specialist I (Lab Technician) - contractor, through CTG, 7/7/2014 -7/22/2016,

Responsibilities include: conduct sample reception, labeling, lyophilization, grinding and extraction; perform analysis of transgenic plants using ELISA to detect the GMO for variety development support and early event characterization ; quantify the protein expression levels in various plant tissues, operate high throughput laboratory equipment, complete data analysis, and deliver results in a specified timeframe, while adhering to quality standards and standard operating procedures; conduct ELISA method validation, write summary and report. Conduct Western Blot to identify specific protein in GMO plant, provide plant molecular breeding support for elite events and variety development to support Bayer Cropscience breeding and trait development product pipelines for soy and cotton for GM and non-GM products.

Professional skills and highlights:

Participated in moderate to extensive problem solving; maintained MicroPlate Readers, Microplate washer, and other general equipment; ensure compliance with all existing Safety, BioSafety, QMS and Stewardship standards. Conduct DNA extraction using automate DNA purification system (KingFisher Flex system), automated liquid handler (Tecan Freedom Evo work station, LGC Meridian liquid dispenser). Protein sample type: plant tissue; plant tissue type: leaf, boll, root and seed.

Minimized lab errors and obtained lab results with consistency and accuracy in a timely manner

Assisted Protein Lead revised Elisa workflow to increase throughput and bring new assays online

Successfully conducted method development and validation for three Elisa assays with minimal supervision

Wrote new assay protocols and revised protocols for exist protein assays

Excellent pipetting skills, and proficiency of using E1-ClipTip multiple channel pipette

Prepare stock protein standard and calibration standards, make extraction and assay buffer

Prioritized and perform multiple tasks using robotics

Proficiency of computer software: MS office and LIMS

Great self-organization, planning, reporting and communication skills

NC DA & CS, Constable Lab (ISO 17025 agricultural lab), 4000 Reedy Creek Road, Raleigh, NC 27607

Chemistry Tech III, 11/04/2002–05/03/2014 (Residue, Food and fertilizer groups)

Duties include: conducting testing and interpreting results of a wide variety of qualitative and quantitative chemical analyses on food, feed, forage, and fertilizer samples in accordance to ISO 17025 standards. Prepare samples, reagents (including Mobile Phase), standard solutions and quality control samples. Operate, calibrate, and troubleshoot laboratory equipment. Track sample backlogs to drive customer required turnaround times, enter and review data, and release final reports in LIMS. Protein sample type: plant, food and feed.

Qing Zhou

Work Experience (continue)

Professional skills and highlights:

Performed incoming inspection analysis of food, feed, forage and fertilizer samples of various sample matrix according to ISO 17025 standards.

Performed sample extraction (including SPE) for various samples and matrixes, such as food, feed, forage, fertilizer and plant material (leaf, boll, corn, cotton seed and soybeans).

Conducted pesticide residue and mycotoxin analysis using HPLC including preparing reagents and mobile phase, calculating test results, and maintained HPLC instrument. Compounds: Glyphosate (Roundup), Carbamate, Paraquat, Diquat, Arsenal, PIX, Thiodicarb, Thiamethoxam, Thiophanate Methyl, and Carbanyl. Sample matrix: water, soil, vegetation, plant material, swab, food, feed, and forage.

Performed sample analysis using HPLC, IC and ELISA for food allergen and mycotoxin tests

Maintained chemical inventories, safely disposing of waste and accurately calibrating laboratory equipment

Trained lab personal to conduct analysis to meet ISO 17025 standards

proficiency of Computer software: MS Office, LabworksLIMS, Agilent Chemstation

*References available upon request.

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