Madhungi (Madhu) Vaidyanathan

813-***-**** ac2cyl@r.postjobfree.com

A dedicated, reliable, friendly, independent, multi-tasker, quick learner and willing to learn more with experience working in academic research skilled in Cell Culture, RT-qPCR, Cell based assays and training. Seeking an exciting and challenging position to execute my scientific knowledge.

Skills & Abilities

Knowledge on ICH-GCP guidelines, regulatory requirements and Drug safety

Cell culture technique- growing cells, monitoring and maintenance, cell viability, cell-based assay(micro plate), in-vitro drug studies, freezing and thawing using aseptic techniques

Knowledge on extraction of proteins and nucleic acids from Tissues.

Tissue Homogenization

Microbiology- staining methods, reading slides in microscope, media preparation, plating, biochemical test and culture methods

SDS-PAGE Electrophoresis

Concentrating Proteins by Chloroform-methanol extraction, for SDS-PAGE

Protein assay (Micro plate assay)

Western Blotting (or Immunoblotting)

RNA isolation and quantitative analysis by RT-qPCR method

Immunohistochemistry & Knowledge on ELISA

Microscopy

UV-VIS Spectrophotometer, nanodrop spectrophotometer

Preparation of buffers, media and solutions and have knowledge of using pH meters

Knowledge of high speed centrifuge

Antimicrobial assay- zone diffusion assay and colony counting method

Blood group analysis, urine analysis

Disposing of hazardous waste and autoclaving

TECHNICAL SKILLS

Knowledge on using Microsoft Word, Excel Spreadsheet, Power Point and Image J

Good organizational and interpersonal skills

Education

CERTIFICATE IN CLINICAL RESEARCH 2017-2018 DURHAM TECHNICAL COMMUNITY COLLEGE, DURHAM, NC. – CURRENTLY ENROLLED

Major: Clinical Research

MASTERS OF SCIENCE 2000-2002 UNIVERSITY OF MADRAS, CHENNAI, INDIA

Major: Biochemistry

POST GRADUATE DIPLOMA 1999-2000 VOLUNTARY HEALTH SERVICES HOSPITAL, CHENNAI, INDIA

Major: Medical Lab Technology

TRAINING AND CERTIFICATION

FDA –Drug safety and efficacy workshop – Certificate – Amedco, Sep 2017

Regulatory Affairs Training – ORAQ – Duke University School of Medicine, Durham, NC (2017)

HPLC and instrumentation preparation and purification of biological samples for analysis- Wake Tech (2013)

Experience

RESEARCH TECHNICIAN OF NEUROBIOLOGY LAB NORTH CAROLINA CENTRAL UNIVERSITY JAN 2016- PRESENT

Responsible for ordering of lab supplies by communicating with Vendors and also involve in creating carts for requisitions.

Discussing with lab members for their needs in supplies and manages and update inventories and documenting any orders. Tracking the orders regularly to ensure if we have received properly.

Responsible for safe keeping the reagents and chemicals considering safety

Conduct research on the effects of alcohol and cannabinoids on neurogenesis and neuro-inflammation and maintain records and report the data to PI

Responsible for maintaining various cell lines and testing them for the effects of drugs

Participate in Lab meetings to assess the data and results of experiments by discussing with the principal investigator and participate in trouble shooting analysis.

Planning and executing experiments following SOPs

Data collection and preparing experimental documentation as per the applicable guidance

Attend seminars and lectures

Perform routine calibration and maintenance of laboratory equipment

Teaching and training molecular biology techniques for undergrad and graduate students, help assist them with ideas and troubleshooting

Disposition of bio hazardous waste

FREELANCE WRITER BIOTECHIN ASIA APRIL 2015- JUNE 2016

Freelance writer contributing on current scientific articles for biotech news website biotechin.asia

RESEARCH ASSOCIATE CENTRAL LEATHER RESEARCH INSTITUTE APRIL 2004- JUNE 2005

Worked in the Department of Biomaterials carrying out work related to Molecular Biology

Collected, dried and lyophilized herbs

Performed anti-microbial activity to check for minimum inhibitory concentration (MIC) on microbes, which was carried out both by zone diffusion assay and colony counting method

LECTURER HINDUSTAN COLLEGE OF ARTS AND SCIENCE JUNE 2002- APRIL 2004

Taught undergraduate and graduate students in Bioorganic Chemistry, Macromolecular Biosynthesis, Clinical Biochemistry & Human Physiology, and Molecular Biology and Genetics

VOLUNTEER EXPERIENCE

Assisted with documenting and filing patient files for a physician’s office in Florida (2012)

Volunteered in the Biology Department at Hofstra University in New York (2006)

Volunteered in the Biology Department at New Island Hospital in Long Island, NY (2006)

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