Microsoft Office Assistant

NEEHARIKA POTHURI

Mobile Phone: 260-***-****

E-mail: *********.**@*****.***

NEEHARIKA POTHURI,MS,B.Tech

**** ********** ****,*******,** 78641

EDUCATION:

Master of Biotechnology and Bioinformatics (GPA-3.7)

California State University Channel Islands California, United States December 2010

Bachelor of Biotechnology (GPA-3.5)

Sree Vidyanikethan engineering college, India April 2008

Work Experience

Manufacturing associate scientist

Agilent Technologies, Inc.

Austin, Texas July 2011-Present

Worked as a production associate in the manufacturing of capture libraries for target enrichment in next generation sequencing.

Duties include:

-Production and testing of Capture Libraries

-Running PCR,QPCR,QRT-PCR

-Purification and quantification of DNA

-Invitro transcription

-RNA purification and quantification

-Production of oligonucloetides using automation technology with the Biocel

-DNA Sample preparation for sequencing

-Troubleshooting technical problems

-Working and maintaining 5S environment

-General laboratory maintenance of stocks of raw materials including reagent and buffer preparation

-Writing, maintenance and reviewing of ISO compliant work instructions and standard operating procedures.

-Working with data based on excel spreadsheets.

RESEARCH EXPERIENCE

Research Assistant December 2010 – June 2011

Dept of molecular pharmacology and physiology

Byrd Alzheimer’s research institute, Tampa, FL

Studied the immunological aspect of Alzheimer’s disease under the supervision of Dr Chuanhai Cao

-Duties included antibody titer determination and Epitope mapping

-Immunostaining,cytokine expression detection

-DNA,RNA extraction and purification

-ELISA, western blot, SDS-page and agarose gel electrophoresis

-IPTG induced gene expression

-Protein extraction and purification

-Determination of protein concentration

-Record keeping, ordering, stocking supplies, reagent and buffer preparation.

-General laboratory maintenance including incubators, water baths, refrigerators, autoclaves, electrophoresis apparatus, and glassware

Research assistant April 2008-November 2008

National institute of oceanography, India

Studied molecular cloning and sequencing of insertional sequence IS21 from indigenous plasmid pCMS1 isolated from the soil bacteria Brevundimonas diminuta under the supervision of Dr. Ramaiah

-Duties included NCBI database searching and blasting of sequences

-Plasmid isolation, Agarose gel electrophoresis,restriction digestion

-ligation, transformation

-DNA purification

-DNA-DNA hybridization technique

-IPTG induced gene expression

-Molecular cloning and sub-cloning

-Primer designing and PCR

TECHNICAL SKILLS

Mammalian Cell Culture – Experience with culturing, proliferation and differentiation of human neural stem cells. Live culture maintenance of other cells lines like CHO, JURKAT, HEK293, HeLa, NIH3T3, 3T3L1, Murine embryonic stem cells and Feeder layers, cell viability assays,medium optimization and microscopic techniques.

Advanced laboratory techniques – Immunofluorescence staining, ELISA, immunodiffusion and immunoelectrophoresis, Invitro transcription RNA silencing and RNA interference, protein crystallography, western blotting and DNA-DNA hybridization.

Immunology: H&E Immunohistochemistry, Fish, Dual color dual fusion methodology, Papanicolau staining, Radial & double immunodiffusion, Immuno-blot, ELISA.

Basic laboratory techniques – Bacterial gene cloning,Sub-cloning,clonal selection, DNA isolation and purification, primer designing, polymerase chain reaction (PCR), recombinant protein expression and purification, protein gel electrophoresis, immunoblotting,protein extraction and purification and electrophoretic techniques(agarose gel electrophoresis,SDS-PAGE)

Computer skills – Database searches (NCBI, PDB), using NCBI applications, Building electron density maps (WINCOOT), Microsoft office applications (Word, PowerPoint, Excel).

ACADEMIC PROJECTS

Designing a research proposal for the treatment of atherosclerosis, under the supervision of Dr. Thomas Schulze PhD, Faculty Advisor. The project involves in finding a novel treatment for atherosclerosis and designing a research proposal describing the treatment and its preclinical studies.

The project involved intensive literature research, patent searches and extensive market research in order to justify our research proposal.

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