Lab Technician Data
Resume:
STACY JOHNSON
** *. ***** **** **. Apt.****
Tucson Arizona 85704
*****@***.***
QUALIFICATIONS SUMMARY
Acute attention to detail, focused, tenaciously strive to achieve goals, former masonry foreman
Years of experience in the workforce, dependable, determined, work well with a team or alone
Keen inductive and deductive reasoning, sharp memory, data processing and computer skills
Former business owner, skilled at advertizing and customer satisfaction
EDUCATION
Northern Arizona University, Flagstaff Arizona
Master of Science, Biology, GPA 3.70, August 2010
Northern Arizona University, Flagstaff Arizona
Bachelor of Science, Cellular and Molecular Biology, Chemistry minor, GPA 3.54, May 2008
Yavapai Community College, Prescott Arizona
Associate of Science, GPA 3.46, August 2005
American Career Builders Truck Driving School, Tucson, Arizona August 2013-October 2013
Student/Graduate. Certificate CDL-A Commercial Truck Driving
HAZMAT
Tanker
Doubles/Triples
EMPLOYMENT
Northwest Colorado Transport
Vac-Truck operator, Killdeer, ND August 2014-Present
Bakken Energy, Miller’s Midnight Trucking March 2014- August 2014
Vac-Truck operator, Williston, Watford City, ND
Reynolds Logistics, Tucson Arizona September 2013-March 2014
Team Over The Road driving
Averaging 2600 miles per week
Doing masonry side jobs on my own, March 2013-August 2013
searching for employment, Tucson Arizona.
Nesco Resources,Ventana Medical Systems, Tucson Arizona October 2012-March 2013
Research Associate R@D
Performed pH measurements on numerous solutions
Prepared reagents in various molar concentrations and dilution percentages
Used Karl Fischer titration Coulometer to determine H20 content in solutions
Collected and then entered large amounts of data via excel spreadsheets
Measured and weighed various mechanical parts for material compatibility
Operated experimental haematoxylin and Eosin staining machines
Ran biopsy specimen slides through H@E machines
Prepared and signed various forms and paper work
Kept meticulous lab book entries
Researched/read peer reviewed scientific journals
Understands and follows good lab practices
Caris Life Sciences, Phoenix, Arizona September 2011-October 2012
Laboratory Aide/Molecular Lab Technician
Performed RNA extraction from patient cancer biopsies via BioRad instruments
Utilized sterile pipepetting skills in multiple daily samples
Quantified/standardized RNA samples via nanodrop BioRad
Synthesized patient DNA via assay using company SOPs
Performed PCR/RT-qPCR
Extracted RNA/DNA from patient blood samples and frozen tissue samples
Analyzed patient RT-qPCR data daily
Used various company computer software programs
Doing masonry side jobs on my own, April 2011-October 2011
searching for employment, Phoenix, Arizona.
A-Tek inc/Homeland Security, State Dept. of Health, Phoenix, Arizona October 2010-April 2011
Microbiologist, Bioterrorism BCL-3 Lab
Performed RNA extraction from filters placed around the city
Maintained Chain of Custody paper work for samples
Utilized sterile pipepetting skills in multiple daily samples
Synthesized DNA from RNA extracted from filter samples
Performed PCR/RT-qPCR
Analyzed samples PCR/RT-qPCR / for presence and concentrations of 5 BCL-3 bacterial pathogens
Used various company computer software programs
Doing masonry side jobs on my own, August 2010-October 2012
searching for employment, Tucson Arizona.
Completing data analysis and thesis writing, Flagstaff Arizona. June 2010-August 2010
Northern Arizona University, Flagstaff Arizona. June 2008- June 2010
Lab technician/Graduate
Maintained cell cultures with Dulbecco’s glucose media, infected cell cultures with protozoan parasites
Extracted mRNA and proteins from cultured cells using appropriate lysis buffers, prepared all buffers
Prepared and loaded lanes after cDNA synthesis and PCR, ran gel electrophoresis via Biorad minicycler
Performed SDS-PAGE and western blot, applied primary & secondary antibodies to PVDF membranes
Prepared ECL reagents for immunoblots, transferred to X-ray film,
Stripped membranes applied anti-actin antibodies, performed RT-PCR via Biorad, standards+melt curve
Used siRNA knockdown transfection kits, entered data in Excel, interpreted data, thesis and manuscript
Taught Bio 100 lab classes
Background in cell biology, microbiology, virological, mycological, and parasitological lab techniques
Performed biochemical and microscopic analyses on purified bacterial and viral cultures
Completing undergraduate college course work, Prescott, Arizona. June 2002-June 2008
Doing masonry side jobs on my own, Prescott, Arizona. November 2001-June 2002
Two home businesses, Keapana Horsemanship trail rides, and September 1996-November 2001
Keapana Services activity referral, Kauai, Hawaii. Co-business owner
Completed business proposal, managed creative advertizing, scheduled trail rides
Fed and watered horses, repaired fences
Referred trairide clients and other visitors to other island activities via website with discount card
Worked on formulating and building Keapana Horsemanship trail rides, and Keapana Services
activity referral businesses March 1996-September 1996
ACE Certified Personal Trainer, Personal December 1993-March 1996
Fitness Trainer, Honolulu, Hawaii.
Instructed clients on fitness machinery, tailored exercise/diet regimes for clients of all ages and genders
Measured heart rates and calculated VO2 max for aerobics clients, underwent CPR certifications
Measured body composition, took blood pressure, rendered encouragement and guidance
ACE Certified Personal Trainer training course studies. November 1992-December 1993
Honolulu, Hawaii
Masonry Construction foreman/supervisor, journeyman brick/block layer May 1977- November 1992
Spread mortar on brick/block using trowel, measured squared and plumbed various masonry structures
Read blueprints for structural steel reinforced commercial buildings, supervised worksite construction
Performed initial layout of numerous commercial and residential buildings, built decorative brick patios
Worked with city inspectors and engineers to ensure structural integrity of block walls
HONORS, AWARDS
Graduated cum laude, Northern Arizona University, August 2010
Awarded government SMART grant 4 times for B+ average in advanced science classes 2007-08
Invited to the Golden Key International Honor Society 2007-10
Dean’s list for 2 semesters Yavapai College
References
Dr. Maribeth Watwood. Professor and chair of the biology department at Northern Arizona University (NAU). ********.*******@***.***. Phone, 928-***-****.
Dr. Catherine Propper. Professor of Endocrinology NAU. *********.*******@***.***. Phone, 928-***-****.
Dr. Bruce Hungate. Professor and chair of environmental science at NAU. *****.*******@***.***
Phone, 928-***-****.
Dr. Nancy Johnson. Professor of environmental science at NAU. *****.*******@***.***. Phone, 928-***-****.
Dr. Fernando Monroy. Professor of Immunology at NAU. ********.******@***.***. Phone, 928-***-****.
Dr. Russel Bull. Vocational Rehab. Phone, 928-***-****. ********@*****.***.
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