Assistant Engineering

Rohit Keshav Rohra

*** ****** ******, ***. #***, College Station, TX – 77840, USA 979-***-**** **********.****@*****.*** Professional Summary

Trained biologist, with intensive training in molecular biology, genomics and application of bioinformatics approaches to large genomic datasets. Seeking new challenging projects in genomic research, analyses and interpretation of complex biological sequence data. Available for new positions starting May 2017. Academic Background

Texas A&M University, College Station, Texas, USA. Aug’15 – May’17 Masters in Biotechnology (GPA – 3.82)

Thadomal Shahani Engineering College, Mumbai, India. June’10 – May’14 Bachelor in Engineering – Biotechnology (GPA – 3.5) Academic Certificates

Drug Discovery and Molecular Modelling (Mumbai, India) April’15 – May’15 Advanced diploma in Life Science Analytics (Mumbai, India) May’15 – June’15 Academic Projects

Quantitative and Functional Genomics Lab (Athrey Lab) – Texas A&M January’ 16 – Current Illumina library preparation for Next Generation Sequencing samples (January’17 – May’17)

§ Gained experience in library preparation for sequencing on Illumina platform. Graduate student researcher (January’16 – June’16)

§ Performed DNA and RNA isolation, purification and quantification using Bioanalyzer, PCR / qPCR technique and electrophoresis techniques.

§ Gained experience in bioinformatics microbiome phylogenetic analysis of laying hens having different levels of circadian rhythm (23Light:1Dark, 12Light:12Dark) using QIIME and MOTHUR. Whole Genome Analyses and variant discovery using re-sequencing data on chickens (June’16 – May’17)

§ Performed variant discovery on short-read data (Illumina) from 17 whole genomes using the GATK Pipeline.

§ Analyses of spatial patterns, effect prediction and population/evolutionary genetic tests from variant datasets. Insilco validation of microRNAs (miRNAs) in the chicken genome (January’16 – March’17)

§ Performed gene mapping algorithm to validate (Insilco) miRNA sequences, predicted to regulate the chicken genome. Conducted mapping of miRNA (original, complementary and reverse complementary) sequences against protein coding regions of chicken genome.

§ Validated miRNA sequences were analyzed for their function on biological processes and microbial pathways regulating chicken protein sequences, using gene ontology analysis. Graduate student assistant Dr. Welsh Lab – Texas A&M August’15 – December’15 Analyzing homologous proteins of Theiler’s virus inducing auto-immune diseases in mice CNS

§ Proteins affected during stress in Theiler’s virus induced mice models were identified and mapped against central nervous proteins in mice, using mapping algorithms, understanding their mechanism and pathways involved.

§ Homologous proteins were further analyzed to determine protein epitope binding sites using Immune Epitope Database and Analysis Resource (IEDB), in order to modulate their immunological function. Under-graduate Final year project – Thadomal Shahani Engineering College October’13 – May’14 Analyses of microbial behavior in probiotics

§ Isolated pure probiotics live cultures from commercial yogurt, E. coli and homemade yogurt bacterium and allowed them to grow under suitable growth conditions.

§ Performed, monitored and compared microbial population dynamics of commercial lactobacilli probiotic microbes and homemade yogurt bacteria, against E. coli under suitable growth condition to investigate the behavior and efficacy of probiotics microorganisms from gut pathogens such as E. coli. Work Experience

Enovate Biolife Pvt. Ltd. (Mumbai, India) November’14 – February’15 Technical Assistant (R&D)

§ Carried out various extraction experimental techniques to obtain biologically active compounds from natural herbs.

§ Performed literature review to generate a list of current extraction methods of biologically active compounds and their relevance in the herbal industry.

Relevant Skills

Bioinformatics BLAST, GATK, BWA, Bowtie, STAR, QIIME, MOTHUR, VELVET, QUAST, kmergenie, IGV, UCSC genome browser, shell scripting, R, JMP, statistics, CASTp and ArgusLab. Wet Lab DNA / RNA isolation and quantification, PCR and qPCR, primer design, Sequencing library preparation, recombinant DNA techniques, SDS-PAGE, Western Blotting, Immunocytochemistry, ELISA, biochemistry downstream extraction, cell culture growth and inoculation. Certificates and Honors

§ Outstanding graduate student of Biotechnology (TAMU) – Spring’17.

§ Blood borne pathogen and CITI responsible conduct of research certificates from TAMU.

§ Proficiency certificate in bio-separation and downstream processing national conference – Mumbai. Work Authorization

§ Authorized to work in USA with OPT and 2 years OPT – extension, until June’20.

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