Curriculum Vitae

BHAVANA H. MANJUNATH

Address for correspondence:

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Morrisville, NC 27560.

Phone: 919-***-****

Email: ac18yz@r.postjobfree.com

U.S. Work Authorization: U.S. Permanent Resident.

Education:

Bachelor of Engineering (B.E.) in Biotechnology June 2009

Bapuji Institute of Engineering and Technology (BIET), Visveswaraiah Technological University, Davanagere, 577004 Karnataka, India (4 year degree).

Marks Scored: Degree Evaluated by ECE – 136 credits completed.

Major Subjects studied during B.E. Program:

Biochemistry, Microbiology, Cell biology and genetics, Structural biology, Molecular biology, Immunology, Genetic Engineering and Applications, Bioinformatics, Enzyme Technology, Genomics and Proteomics, Health and Pharmaceutical Biotechnology, Separation Techniques, Upstream and Downstream Process Technology, Microbial Biotechnology, Food and Agricultural Biotechnology, Nanobiotechnology, Lab to industrial scaling (biotechnology product development), Industrial Management, Structural biology, Bioenergetics, Bioprocess Principles and Calculations, Bioethics and Biosafety, Bioprocess Equipment Design and Drawing, Bioprocess and Bioreaction Engineering.

Techniques learnt during B.E. Program:

Electrophoretic and chromatographic techniques.

Colorimetry.

Enzyme assays.

Enzymology experiments.

Molecular biology experiments.

Cell separation techniques by centrifugation and sonometer.

UV-Spectrophotometry.

Qualitative and quantitative estimations of biomolecules.

Recombinant DNA technology.

Wine preparation using Rotary shaker.

Sterilization techniques like Hot air oven, Autoclave, and Air filters.

Preparation of culture media, culturing of microbes, isolation and enumeration of microbes.

Gram staining methods of bacteria.

Microscopic observations of Prokaryotes and Eukaryotes.

Project during B.E Program:

Jointly undertook a research project on “Biotechnological process of composting and field evaluation studies”, under the guidance of Dr. Rangaswamy, Dr. Ravishankar, and Mr. Gangaraju at BIET, Davanagere.

Summary of the project: This study examined the impact of vermicompost on tomato plants. The study involved production of vermicompost using vegetable waste collected from the students’ hostels, which was decomposed using earthworms (Eisenia fetida) in constructed pits. Three separate experimental plots were prepared and planted with tomato crop. While one plot was treated with vermicompost, the other two plots were treated for chemical fertilizer and control. Several indicators of soil fertility such as PH, nitrogen, phosphorous, potash, and organic carbon were measured before and after the treatment. A comparison of tomato yield indicated that plants grown with vermicompost had 40% and 133% higher yields compared to chemical fertilizer and control plots, respectively. The study concluded that application of vermicompost is much better than chemical fertilizers for tomato crop.

Professional Training:

Undertook training courses in Shreedhar Bhat’s Laboratory on “Genetic Manipulation of Tobacco Plant with Annexin Gene through Agrobacterium Mediated Transformation and Immunology”, during April–May, 2007. Hands on experience on following techniques were acquired during the training program:

Isolation of plasmid.

Agarose gel electrophoresis.

Restriction digestion, gel elution, ligation.

Agrobacterium plasmid isolation and transformation.

Co-cultivation of tomato leaf discs with Agrobacterium tumefaciens.

Screening of transformants.

Polymerase Chain Reaction (PCR).

Enzyme- linked Immunosorbent Assay (ELISA).

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).

Western Blotting.

Immunodetection.

Computer Knowledge: MS Office suite.

Language Proficiency: English, Kannada, Hindi.

References:

Dr. B.G. Pujar

Professor & Head

Dept. of Biotechnology,

BIET

Davangere – 577004, India.

ac18yz@r.postjobfree.com

Dr. B.E. Rangaswamy

Assistant Professor

Dept. of Biotechnology,

BIET

Davangere – 577004, India. ac18yz@r.postjobfree.com

Mr. N.S. Manjunath

Senior Lecturer

Dept. of Biotechnology

BIET

Davangere – 577004, India. ac18yz@r.postjobfree.com

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