Collecting samples and analysis

Neeraja Sarikonda

832-***-**** (Cell)

**********@*****.***

*** ****** **** **, **** Chester, PA 19380

Summary : Looking for entry level or part time position in the field of science in west Chester,PA.

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Work Status : Permanent Resident (Green card)

Work Experience :

Cytogenetic Technologist October 2010 – July2011

Department of Molecular Cyto Genetics

Thomas Jefferson University Hospital, Philadelphia

All of my work in Cyto genetics Department is, processing the Samples including culture set-up, harvesting, staining followed by analysis, Under the guidance of senior technologist started analyzing cases.

Research Assistant March 2010 – Sep2010

Department of Dermatology, cell and Developmental Biology,

University of Pennsylvania, Philadelphia

All of my work in Dermatology Department is, To Make a Mouse tail Genotyping Using PCR, ImmunoFluorescent, Immunohisto Chemistry, ImmunoFlurorescence on cells Tranfection, Viral Transduction, Cell Culture,RNA, Protein extraction and quantification,Harvesting both embryos and adult skin tissue, DNA extraction, Real time PCR Min,mid plasmid purification and Western Blotting.

Research Assistant (Volunteer) March 2006 – July 2006

Department of Biology,

University of Pennsylvania, Philadelphia

Analysis of Transgenic plants ‘Phenotype’

Work involved the model plant Arabidopsis thaliana, utilized a combination of genetic, molecular, and biochemical approaches. Planting, harvesting, seeds are introduced in to kanamysin sulphate mediam and identify the phenotype.

Yeast 2 hybrid to confirm the putative signaling player’s, Yeast and Escherichia Coli plasmid DNA purification using Wizard plus SV minipreps purification system, QIAGEN plasmid kit, PCR Purification kit and template preparation.

One step Transformation of Yeast in stationary, Agarose gel electrophoresis, Illumination of DNA from The gel using Gel extraction kit protocol. Liquid culture assay using ONPG as substrate.

Course Contents:

- Molecular Genetics, Cytogenetics, Cell Biology and Cellular Biochemistry

- Mechanics of inheritance in prokaryotes and eukaryotes genetics

- Eco Genetics, Human Genetics, Recombinant DNA technology

- Biostatistics and Computer Applications

Laboratory Skills:

Studied toxicity effects of chemicals like ethidium bromide on mice by micronucleus and sperm abnormality assay. Mitotic Studies in Swiss albino mouse bone marrow cells Allium cepa root tips, Tulsi and Cisplatin (using with EB), flower buds using with Aceto orcein and Feulgen stains and grasshopper and Mouse testis using with Hematoxylin and Giemsa stain. Basic molecular biology techniques like electrophoresis and Mini, Midi and plasmid DNA extraction.

Education : MS in Genetics

Madras University, India.

BS in Botany, Zoology and Chemistry

Osmania University, India.

IT Skills:

-MS Office Suite (MS Excel, Word, and PowerPoint).

References will be provided on request.

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