Ashley M. Busse

***** *********** ** **

Coon Rapids, MN 55433

(763) 350 – 1722

ac0xoe@r.postjobfree.com

Profile

Recently graduated science technician seeking position in a biology related field to further knowledge in systems and procedures relating to biology. Experience in microbiology research techniques along with chemistry background. Proficient in handling diverse situations as well as resolving various issues as they may arise. Skills with attention to detail, organizational habits and working independently as well as in a team.

Education

Bachelor of Science in Biomedical Sciences, M ay 2016 Saint Cloud State University, Saint Cloud, MN

Experience

Cargill Industrial Services - Plymouth, MN

Contractor through Synectics M ay 1st - Current

● Prepared asphalt combined with additive to find ratio that was most suited to where it would be.

● Used Bending Beam Rheometer to test deflection and relaxation of different asphalt samples and the relationship to different temperatures they were exposed to.

● Utilized viscosity reading, pH, flash point, pour point and density to determine what type of asphalt was which.

● Knowledge of machines such as Bending Beam Rheometer, Vacuum Degassing Oven and Pressure Aging Vessel.

● Conducted experiments to find out what type of candle wax would work best with Micro and different properties.

Eurofins DQCI – Mounds View, MN

Contractor through Aerotek N ovember 25t h

– February 3rd

● Sorted dairy samples to determine what test/machine the samples will be run on.

● Ability to run the FT-120 machine and follow the procedure given to test certain samples such as heavy cream.

● Knowledge of how to run the combi-machine and how to arrange the samples on the machine.

● Worked with technicians running ELISA testing, pregnancy testing and other quality control tests.

Center for Food Safety and Applied Nutrition – College Park, MD Food and Drug Administration

ORISE Fellow, Molecular Methods and Subtyping Branch J une 1s t 2016 – August 31s t

● Enumeration and treatment of Listeria monocytogenes through plate readers and antibiotics.

● Inoculation of a stainless-steel plates for growth of gram positive bacteria.

● Efficiency of pipetting microliter concentrations of infectious material with P2-P1000 micropipettes.

● Measured concentrations on Qubit 2.0 with Qubit dsDNA HS buffer and standards

● Extraction of DNA from Salmonella, Listeria monocytogenes, Campylobacter and Escherichia coli using the Qiagen DNeasy Blood & Tissue Kit from Gram and Cells.

● Library preparation of Listeria monocytogenes and Salmonella with Nextera XT Prep Kit.

● Operation of MiSeq control software, Thermofisher SimpliAmp Thermal Cycler and Spiral Platers.

Dr. Gardner Research Group, Chemistry and Biochemistry Research Associate January 2016 – May 2016

● Read literature to ascertain best method to synthesis products.

● Experiment with synthesizing pthalocyanines with different reagents.

● Manipulate various methods such as refluxing, vacuum filtrating, crystallizing and pipetting.

● Maintain a lab notebook to record procedures and reagents used along with any changes in protocol as deemed necessary.

● Attend weekly meetings on discussion of results and how to proceed from that point. Saint Cloud State University – Saint Cloud, MN

Wick Science Building

Undergraduate M ay 2014 – May 2016

● Experiments that affect the catalytic nature of enzymes and ways to analyze the results.

● Exploited behaviors to separate, clean and analyze DNA fragments.

● Propagation of aseptic laboratory techniques.

● Able to quickly diagnose bacteria with different techniques.

● Separated proteins by electrophoresis in a support medium.

● Used techniques to decrease the concentration of a substance in a series.

● Monitored the pH of a given volume of amino acid solution after addition of an alkali.

● Used Bradford assay to determine protein content of samples.

● Able to distinguish between capsular material from bacterial cells.

● Expertise in determining the motility of living bacteria.

● Technique expertise in streaking to isolate a pure strain of bacteria from a single species.

● Use of fractional crystallization to purify an impure compound in a solvent.

● Use of column chromatography to purify individual chemical compounds from mixtures.

● Determination of inhibitor concentrations and behaviors associated with it.

● Worked with centrifuges, pipets and basic chemistry tools.

● Handled specimens such as blood, urine and feces. Organizations

Saint Cloud State University – St. Cloud, MN

American Society for Microbiology

Public Relations Officer, A ugust 2015 – May 2016

● Assisted in presentations along with speeches for fellow undergraduates.

● Coordinated speeches given by professionals in industry.

● Prepared nourishment during various events.

● Attended board meetings to discuss events and different routes the students could benefit from the organization.

● Gained funds to contribute to the students at the university and educate them in different fields.

● Informed students on microbial agents and how to sterilize them utilizing tests used in clinics and hospitals to diagnose disease.

References available upon request

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