***** **** ****** ****

Valley Center, CA *****

760-***-**** ac0tzm@r.postjobfree.com

MARGARET OAKLEY

OBJECTIVE:

Experienced Clinical Laboratory Scientist skilled in performing complex assay testing and supervisory functions. Seeking a position as a laboratory professional in a patient focused and team orientated environment.

QUALIFICATIONS:

Familiar with clinical laboratory instrumentation, including HPLC, LC/MS, RT- PCR, Flow Cytometry, Hematology, Coagulation, Chemistry, Tissue Immunofluorescence/Elisa platform and Electro chemiluminescence.

Skilled in testing monoclonal antibodies by RIA and spectrophotometric methods in an Industrial Quality Control Laboratory.

Proficient in Microsoft office, including excel and word.

Familiar with Statlia, Labdaq, Quicklab, Liras, FCS Express and Fluidigm software.

Performed complex chemical, biological, hematological, immunologic and microscopic tests

LICENSES:

American Society of Clinical Pathologist (ASCP), Medical Technologist

State of California, Department of Health Services, Clinical Laboratory

Scientist (CLS), Generalist

State of Nevada, Department of Health and Human Services, Clinical Laboratory Technologist

EXPERIENCE:

2014- 04/2017 DermTech International La Jolla, CA

Technical Supervisor

Initiated and revised SOP documents per department needs

Assisted in the design of an inventory system for both Pre / Post molecular labs

Prepared and validated test reagents and control material

Interacted and informed upper management of daily laboratory operations

Collaborated with R&D in assay optimization

Performed RNA extraction and qPCR on test samples to screen for melanoma

Performed data analysis

Performed assay validations

Performed routine maintenance and calibration on instruments

Participated in CLIA, CAP and New York inspections

Implemented policies and procedures for CLIA, CAP and New York compliance.

Trained and supervised licensed personnel

Proficient in Excel and Word for building documents and spreadsheets

Participated and presented monthly QC data during QA meetings

2013-2014 MILLENNIUM LABORATORIES San Diego, CA

Clinical Laboratory Scientist-Genetics

Perform data analysis to determine the haplotypes for various metabolic phenotypes using Fluidigm

software

Performed daily review and monthly computation of temperature sensors for the Genetic labs

Familiar with the Fluidigm Biomark system for real-time PCR

Analyzed data for SNP genotyping and copy number variation using digital array IFC

instrumentation for the CYP2D6 gene

Performed Pre-Amplification process for patient assay batches

2012-2013 GENOPTIX Carlsbad, CA

Senior Clinical Laboratory Scientist

Perform patient data analysis for the diagnosis of hematologic malignancies

Utilize FCS Express software for analysis from the BD Canto Flow Cytometry instruments

Review and calculate monthly Coulter Z1 cell counter Quality Control

Perform monthly maintenance and calibration on Coulter Z1 cell counters

Rotated between the various sample prep stations such as cell counting, pipetting and lysing

Assisted in daily maintenance and shut down of BD instruments

2010-2012 MILLENNIUM LABORATORIES San Diego, CA

Clinical Laboratory Scientist II

Reviewed Chromatographs from Liquid Chromatography and Mass Spectrophotometry instruments

Performed data review for Toxicology reports that interfaced with the Laboratory Information System

Reviewed and reported an average of 700-800 Toxicology reports per day for a 24-hour TAT

Collaborated with Quality Control Analysts in the data analysis process

2004 –2010 PROMETHEUS LABORATORIES San Diego, CA.

Lead Clinical Laboratory Scientist

Performed high complexity testing in the areas of Autoimmune, Genetics and Immunochemistry to

aid in the diagnosis of Crohn’s and Celiac disease

Technical Lead of the IBS Bench which included maintaining instruments, Quality Control

monitoring and Parallel Run Data Calculations

Evaluated and report data obtained from HPLC Analyzers used for monitoring drug metabolites

Performed HLA typing using the SSOP assay method

Performed real-time PCR to determine genotypes to aid in the diagnosis Crohn’s and Celiac

Disease

Performed and analyzed immunofluorescence assays

Performed preventative maintenance on fluorescent microscopes and various robotic auto analyzers

Executed and evaluate high volume ELISA assays for various test panels

Review and revise various standard operating procedures to comply with CAP, CLIA and NewYork

State regulations

Familiar with various instruments such as the Qiagen BioRobot, Tecan Auto-Lipa, Tecan Genesis, HPLC, Perkin Elmer Janus, MSD Sector Imager 6000, BD Probetec ET and the ABI Prism 7000

Trained lab personnel in assay set up and procedures

Performed reagent parallel testing for various assays

2003 UCSD IMMUNOGENETICS & TRANSPLANTATION LAB

La Jolla, CA. Clinical Laboratory Scientist II (Part-Time)

Performed infectious disease serology for cadaver organ donation

Performed and analyzed infectious serology assays for donor/recipient

compatibility

Effectively communicated test results with hospital nursing staff

Consistently complied with laboratory policies and procedures in the

maintenance and calibration of lab instruments and the decontamination

of work stations

Compiled monthly quality control data for department review

1986-2000 THE SCRIPPS RESEARCH INSTITUTE La Jolla, CA.

Reference Laboratory, Senior Clinical Laboratory Scientist

Performed department assays in Coagulation, Autoimmune and Immunochemistry

Performed test methodologies using Flow Cytometry, Immunoflourescense, RIA and

ELISA

Maintained and troubleshot lab equipment

Evaluated and conducted daily and monthly quality control

Reviewed and edited standard operating procedures

Performed preventive maintenance and pipette calibration

Prepared materials used for controls and standards for use in ELISA assays

1984-1986 HYBRITECH INC. San Diego, CA

Quality Control Analyst II

Performed product testing starting from raw materials to finished product used in the manufacturing of assay test kits

Interfaced between the Production Laboratory and the Client Service Department

Responsible for monthly quality control data analysis used to monitor major test kits components

1982-1984 PROVIDENCE MEMORIAL HOSPITAL El Paso, Texas

Medical Technologist II

Performed routine and special testing in Chemistry, Hematology, Serology, Urinalysis and Microbiology

Performed instrument maintenance and trouble-shooting

Communicated with physicians and nursing staff regarding patient test data

Performed Phlebotomy when staffing was limited

EDUCATION:

B.S. Medical Technology, University of Texas at El Paso.

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