Moneim Shamloul, PhD

Newark, Delaware *****

302-***-**** • ac0q2u@r.postjobfree.com

Senior Scientist

Skilled scientist with notable success in unveiling new discoveries in virology and botany.

Aggressively executed and developed molecular-based assays to detect viruses, viroids, and other pathogens. Technically cultured professional that has embraced the beauty of plant pathology and the knowledge of genetics through the execution of numerous pre-clinical studies. Performed and developed molecular-based assays to reveal viruses, viroids and other pathologies. Utilize genetic recombination to create a classification of plants that could be used industry-wide to produce vaccine and therapeutic protein. Supervised and provided guidance to laboratory technicians for establishing a uniform, knowledgeable team of researchers.

Core Competencies:

Botany/Virology/Pathology

Assay Development

Risk Assessments

Pre-Clinical Trial Designer

NIDCR-NIH Experience

Quality Assurance and Testing

ARS- USDA Experience

Scientific Researcher

Serve as Peer Reviewer

Teaching experience of Plant Pathology, Virology, and taxonomy

Professional Experience

Fraunhofer Center for Molecular Biotechnology, Newark, Delaware

Senior Scientist (Head of Discovery Group), 10/2014 – 10/2016

Managed antimicrobial mining and optimized fermentation conditions. As lead scientist, ensured that all research completed met standards and laboratory operating expectations. Successfully fashioned Provided direction for all laboratory technicians and staff members driving success by setting targeted goals.

Key Projects:

Discovered an antimicrobial compound utilizing quorum sensing that led to the recognition of how bacteria communicate to express resistance against Gram-positive multidrug resistance bacteria.

Planned and enhanced the making of antimicrobial peptide.

Executed the Western blotting technique, biochemical assays, and SDS-PAGE. Designed to quantitatively analyze recombinant proteins produced using plant-based expression system.

Development a plant transgenic line expressing functional T7 RNA polymerase enzyme.

Fraunhofer Center for Molecular Biotechnology, Newark, Delaware

Senior Scientist (Head of fermentation and expression group), 7/2011 – 10/2014

Demonstrated leadership by utilizing Fraunhofer-CMB’s technological base to create a controlled plant system that would be appreciated industry-wide as the go to method for developing vaccines and therapeutic proteins. Monitored the process of agrobacterium Research Cell Bank (RCB) and how it transferred to Fh-CMB cGMP for the production of a bacterial Master and Working Cell Bank.

Key Achievements:

Spear-headed the development of detecting viral assays for plant seed stock production.

Ensured that all developments and processes were carried out efficiently for the scaling of Agrobacterium fermentations.

Supervised technical staff daily to guarantee that microbiological colonies were created and screenings were completed that would produce an outcome that targeted protein expression.

Fraunhofer Center for Molecular Biotechnology, Newark, Delaware

Scientist (Lead agroinfiltration group), 1/2007 – 7/2011

Managed the agroinfiltration team of scientists that assisted with requesting infiltration materials for downstream processing. Successfully developed an assay that detects seed-borne viruses on the N. benthaniana seed resulting in the increase of virus-free seeds for trial facility creation.

Key Achievement:

Led the development of Agrobacterium high-cell-density cultivation (HCDC).

Key scientist collaborated with engineers on designing a first automated plant facility for vaccines and therapeutic protein production.

Fraunhofer Center for Molecular Biotechnology, Newark, Delaware

Research Associate, 8/2002 – 1/2007

Interest in plant growth in non-soil media led to the creation of a hydroponic and cascading system. Manufactured and improved conditions such as nutrition, light intensity, photoperiod, and more to facilitate transient recombinant protein expression.

Key Achievement:

Caused the result of the purified IA2 protein (insulinoma-associated tyrosine phosphatase), a Type-1 diabetes autoantigen to be transiently expressed.

Research Fellow, NIH, Bethesda, MD 03/2000-07/2002

• Developed a novel multiplex RT-PCR probe capture hybridization for the detection of six viroids in four different genera: Apscaviroid, Hostuviroid, Pelamoviroid, and Pospiviroid simultaneously.

• Characterize a new variant of apple scar skin viroid associated with pear fruit crinkle disease. The new sequence deposited in NCBI database.

• Development a multiplex RT-PCR-ELISA assay to diagnose and identify genotypes of Hepatitis C Virus.

• Development a sensitive, accurate, and reproducible quantitative assay for HBV DNA using real-time PCR based on Lux primer chemistry.

Visiting Scientist, USDA/ARS, Beltsville, MD 06/1998-02/2000

Aggressively executed and developed molecular-based assays to detect viruses, viroids, and other pathogens.

• Established a detection method of the Egyptian species of sugarcane streak virus using PCR-probe capture hybridization and its complete nucleotide sequence.

• Identified a diagnostic banana bunchy top and fava bean necrotic yellow viruses from infected leaves, tissue cultures and viruliferous aphids.

Patent

Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems. Fraunhofer USA. April 5, 2010: 201********

Provisional patent:

Transcomplementation system for target gene expression in plants.

Nucleic acid delivery into plant cells by plasmolysis

Education and Credentials

Doctorate of Philosophy, Molecular Virology Masters of Science, Botany

South Valley University, Egypt Assiut University, Egypt

Bachelor of Science, Botany

Assiut University, Egypt

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