EDUCATION

****-**** *** ****. ** Genetics and Development, Universidad de Zaragoza, Spain (Cum Laude)

****-**** ****** ****. ***** Management, Universidad Internacional de Andalucia, Spain (Cum Laude)

1993-1999 BSc. Hons. Biology (5 years), Universidad de Oriente, Venezuela (Cum Laude)

FELLOWSHIPS

2012 Fellowhip-grant NIH Postdoctoral Associate at Department of Neurology, McKnight Brain Institute, College of

Medicine, University of Florida, Gainesville, FL, USA

2005 Fellowship-contract Dirección General de Aragón-European Union, Spain for PhD in Genetics and Development

2003 Fellowship from Dirección General de Aragón-European Union, Spain for PhD in Genetics and Development

2002 Fellowship for Biotechnology (PhD) Santander- Central-Hispano Bank and Universidad de Zaragoza, Spaint

2001 Fellowship from Universidad Internacional de Andalucía, Spain (Master in Waste Management)

1989 Scholarship from the Spanish Government until 1989, secondary “Colegio Carmelitas”, Orense, Spain.

HONOURS

2007 Award of Merit “City of Porlamar” (Margarita Island Government)

2005 Cum Laude, PhD Genetics and Development

2003 Cum Laude, Master in Waste Management

1999 Cum Laude, BSc. Marine Biology

EMPLOYMENT

2016 Assistant Scientist, Dr. Borchelt’s Lab, Department of Neuroscience, Center for Translational Research in

Neurodegenerative Disease, University of Florida, Gainesville, FL

2014-2016 Postdoctoral Associate, Dr. Borchelt’s Lab, Department of Neuroscience, Center for Translational Research in

Neurodegenerative Disease, University of Florida, Gainesville, FL

2012-2014 Postdoctoral Associate, NIH Grant Support. Department of Neurology, McKnight Brain Institute, University of

Florida, Gainesville, FL.

2007-2010 Research Coordinator, UNEFA (Universidad Nacional Experimental de la Fuerza Armada), Venezuela

2007 Researcher at Environmental Research Regional Center, Universidad de Oriente, Venezuela

2007 Assistant Professor “Contamination Control”, “Marine Resources Conservation”, “Parasitology, Pathology

and Profilaxis of Marine Organisms”, “Environmental Impact”, Universidad de Oriente and UNEFA,

Venezuela

2006 Researcher at Department Neurobiology, Universidad de Zaragoza, Spain

1999-2000 Lecturer assistant “Research Methodology”, “Biochemistry”, “Animal Physiology”, Universidad de Oriente,

Venezuela

1999 Technician Environment Research Regional Center, Universidad de Oriente, Venezuela

1997 Teaching assistant, Universidad de Oriente, Venezuela

RESEARCH PROJECTS

2016 “Investigating the role of MATR3 in RNA metabolism and neurodegenerative disease” (P.I. Dr. David Borchelt,

Dpt Neurosciences, CTRND, University of Florida, Gainesville, Fl, USA)

2014 New Models to Assay Gene Silencing Therapies (P.I. Dr. David Borchelt, Dpt Neurosciences, CTRND,

University of Florida, Gainesville, Fl, USA)

2012-14 Project Ataxia SCA13 NIH Grant, Department of Neurology, McNight Brain Institute, College of Medicine,

University of Florida, Gainesville, Fl, USA

2007 Project “Molecular machinery of Alzheimer Disease in dolphin as a cause of stranding”, UDO, Venezuela

2002 Researcher “Molecular machinery of Alzheimer Disease in dog as an animal model”, “Molecular machinery of

Alzheimer Disease in chicken as an animal model”. “Molecular machinery of Alzheimer Disease in dolphin”,

I+D (Spain) and European Union Funds-Universidad de Zaragoza, Spain

2001 Researcher “Study of physical-chemical and bacteriological condition of areas moresusceptible for environmental

degradation in Nueva Esparta State”, UDO, Council of Research Scientific, Venezuela

COMPUTER SKILLS

Word, Power Point, Corel Draw, Photoshop, confocal and live imaging softwares, Microsoft Windows/MacOS.Primer3, UCSC Genome Compiler, Genome browser, Benchling.

TECHNICAL SKILLS AND COMPETENCES

Cloning, CRISPR/Cas9 design, live imaging, confocal, primer design, RACE, paraffin embedding and sections brain tissue, DNA extraction from fresh and paraffined tissue, DNA purification, electrophoresis, cell culture, transfection, growth cell curve, cell culture and maintenance of different cell lines (COS1, SHSY5Y, HEK293, A549, HFL, H4, CHO, 3T3, N2A, HeLa, C2C12, primary culture, co-culture), ligation, transformation mini/maxi, transient and stable transfeccion in vitro, transduction AAV, apoptosis and stress indcution, reverse transcription to cDNA, PCR, Real Time PCR, restriction enzymes, Western Blot, isolation of genomic DNA, isolation of total RNA, LIBS (LaserInduced Breakdown Spectroscopy) applied to tissues, fluorescence microscopy, live imaging, FACS sorting, CRISPR/Cas9 design guides and cloning into Cas9 vectors, CRISPR in vitro experiments and sruveryor assays, immunohistochemistry, immune-cytochemistry.

OTHER TECHNICAL SKILLS

Waste treatment project design. Water chemistry as DQO, DBO, nitrogen, phosphorous, surfactants, aromatics hydrocarbons, heavy metals, oil and grease, solids

SCIENTIFIC COMUNICATIONS

2017 The binding of RNA regulates the formation of nuclear membraneless structures by Matrin3 and TDP43 in myocytes. Phase Transitions in Biology and Disease conference. VIB (Vlaams Institut voor Biotechnologie) and KU (Katholieke Universiteit Leuven), Leuven, Belgium.

2015 Analysis of Matrin-3 enconding mutations associated with ALS in cultured cell models. C. Gallego-Iradi, M. Pace, J. Lewis, D.R. Borchelt. Neuroscience 2015, Society for Neuroscience SfN, Chicago, USA.

2015 Heterogeneity of Matrin3 expression in the developing and murine central nervous system. Rayaprolu, S, D'Alton S,’Howard, J, Moloney, C, Rosen, A, Iradi, M, Crosby, K, Borchelt, D, Lewis, J. MND Symp. Orlando, FL, USA.

2015 Levels of ALS-associated protein Matrin3 are indirectly proportional to disease vulnerability. Rayaprolu, S, D'Alton S,’Howard, J, Moloney, C, Rosen, A, Iradi, M, Crosby, K, Borchelt, D, Lewis, J. Center for Translational Research in Neurodegenerative Disease and Department of Neuroscience, College of Medicine, University of Florida, Gainesville, Florida, USA.

2007 Speaker “Strategic Management of Genetic Information” conference Militar Hospital, Porlamar, Venezuela.

2006 “Alzheimer-like neurodegeneration as a probable cause of cetacean stranding”. Sarasa M., Gallego M.C., Federation of European Neurosciences Societies (F.E.N.S.), Austria

2005 Molecular machinery of Alzheimer Disease in Dolphin”. Sarasa M., Gallego M.C., XI Congreso de la Sociedad Española de Neurociencia, Madrid, Spain

2000 EXPOMERCK 2000-Universidad Santa María, Caracas, Venezuela.

1999 “Study of physical-chemical and bacteriological characteristics of the effluent of a semi{intensive culture of white shrimp, Litopenaeus vannamei”, Aquaculture Congress, Puerto La Cruz, Venezuela.

1999 EUREKA 99 “Alternatives for conservation of the aquatic park Laguna de La Restinga, Caracas, Venezuela.

1994 I Congress of Aquatic Sciences, Margarita Island, Venezuela.

COURSES

2003 “Biology, Medicine and Conservation of Marine Mammals”, Universidad Autónoma de Barcelona,Spain

2002 Proffesor Training Course (one year), Universidad de Zaragoza, Spain

2000 “Environmental Impact Course”, UDO-Universidad Las Palmas de Gran Canaria

2000 “Laboratory Reagents Storage and Management”, MERCK, Venezuela

1999 “Professional Qualification and Improvement in Physicochemical and Bacteriological Water Analysis Techniques”, environmental Research Regional Center, Margarita Island (Venezuela)

1999 Environmental Management” (IICA)-Gesellschaft Technische Zusammenarbeit (GTZ), Maturin, Venezuela

PUBLICATIONS

2017 Oksana Kaidanovich-Beili, María Carolina Gallego Iradi, Danielle Gunn-Moore, Frank Gunn-Moore and Simon Lovestone. Alzheimer’s disease in cetaceans and humans; a consequence of longevity rather than ageing (submitted to Alzheimer’s & Dementia).

2017 M. Carolina Gallego-Iradi, Rachel Davila, Anthony Crown, Hilda Brown, Jada Lewis, David Borchelt RNA binding regulates the formation of nuclear membraneless structures by Matrin 3 and TDP 43 (under review by Journal of Cell Biology).

2016 Sruti Rayaprolu, Simon D’Alton, Keith Crosby, Christina Moloney, Abigail R Hernandez, John Howard, Carolina Gallego-Iradi, David Borchelt and Jada Lewis. Heterogeneity of Matrin3 in the developing and aging murine central nervous system. Journal of Comparative Neurology PMID:26878116

2015 M Carolina Gallego-Iradi, Alexis Claire, Hilda H Brown, Christopher Janus, Jada Lewis, David R Borchelt. Subcellular localization of Matrin 3 containing mutations associated with ALS and distal myopathy. PLoS ONE 10(11). PMID:26528920

2014 Gallego-Iradi C, Bickford JS, Khare S, Hall A, Nick J, Salmasinia D, Wawrowsky K, Bannykh S, Huynh D, Rincon-Limas D, Pulst S, Nick H, Fernandez-Funez P, Waters M. Aberrant intracellular trafficking of KCNC3-R420H: a K+ channel mutation causative in spinocerebellar ataxia 13. NEUROBIOLOGY OF DISEASE, 71: 270-9

2010 Sarasa L.,Gallego C., Monleón I., Olvera A., Canudas J., Montañés M., Pesini P. and Sarasa M. Cloning, sequencing and expression of the main APP isoforms and the enzymes related with their processing in the dog NEUROSCIENCE, 171 (4): 1091-1101

Genbank sequences:

Gallego, C. and Sarasa, M. Grampus griseus amyloid beta (Aß) protein, partial cds. AY926589.

Gallego, C. and Sarasa, M. Tursiops truncatus amyloid beta (Aß) protein, partial cds. AY926588.

Gallego, C. and Sarasa, M. Stenella coeruleoalba presenilin 2 mRNA, partial cds. AY926586.

Gallego, C. and Sarasa, M. Stenella coeruleoalba presenilin 1 mRNA, partial cds. AY926585.

Gallego, C. and Sarasa, M. Stenella coeruleoalba BACE mRNA, partial cds. AY926584.

Gallego, C. and Sarasa, M. Stenella coeruleoalba beta-amyloid precursor protein mRNA, complete cds. AY926583.

Gallego, C. and Sarasa, M. Canis familiaris apolipoprotein E (ApoE) mRNA, partial cds. AY926587

Gallego, C., Sanchez-Diaz, R., Sarasa, L. and Sarasa, M. Canis familiaris beta-amyloid precursor protein 695 (APP-695) mRNA, complete cds. AY926582.

Gallego, C., Sanchez-Diaz, R., Sarasa, L. and Sarasa, M. Canis familiaris beta-amyloid precursor protein 714 (APP-714) mRNA, complete cds. AY926581.

Gallego,C., Sanchez-Diaz,R., Sarasa,L. and Sarasa,M. Canis lupus familiaris beta-amyloid precursor protein 770 mRNA, complete cds. AY926579

Gallego,C., Sanchez-Diaz,R., Sarasa,L. and Sarasa,M. Canis lupus familiaris beta-amyloid precursor protein 751 mRNA, complete cds. AY926580

Sarasa,L., Gallego,C., Monleon,I., Olvera,A., Canudas,J.,Montanes,M., Pesini,P. and Sarasa,M. Canis lupus familiaris ADAM metallopeptidase domain 17 (ADAM17), transcript variant 1, mRNA. NM_001286866

Sarasa,L., Gallego,C., Monleon,I., Olvera,A., Canudas,J., Montanes,M., Pesini,P. and Sarasa,M. Canis lupus familiaris amyloid beta (A4) precursor protein (APP), mRNA. NM_001006601

Sarasa,L., Gallego,C., Monleon,I., Olvera,A., Canudas,J., Montanes,M., Pesini,P. and Sarasa,M. Canis lupus familiaris presenilin 2 (Alzheimer disease 4) (PSEN2), mRNA. NM_001284465

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