Medical Professional Experience
Resume:
Zhongxian Joseph Huang
********@*****.*********:********@*****.***
SUMMARY
A highly efficient and productive senior research associate with extensive expertise in medical, biotechnology and pharmaceutical research fields. Possess a strong work ethic and the ability to rapidly assimilate new information and create commercially valuable products.
PROFESSIONAL EXPERIENCE
Becton, Dickinson and Company Biosciences Pharmingen, San Diego, CA
Senior Research Associate
●BD DimerX Antigen Presentation Reagent Projects: Released 11 products, Finished 3 products.
●Phosflow Projects: Developed 43 fluorescent, phosphorylation site-specific monoclonal Abs, for the analysis of cell signaling by multicolor flow cytometric analysis.
●Earned a Superior Accomplishment Recognition Award one of only two employees to receive this recognition in 2000 and this award is the first time to issue in the company.
●Involved in the work leading to the publication of four abstracts (Abstracts 1, 2, 3, 4) and Posters that were presented at National Scientific Meeting.
University of British Columbia, Vancouver, BC Canada
Research Fellow
Department of Pharmacology & Therapeutics (1993–1998)
●Isolated single rat myocytes for anti-arrhythmic drug electric physiological studies.
●In vivo and in vitro rat heart model systems for anti-arrhythmic drug studies
MRC Peptide Group, Department of Physiology (1990–1992)
●Made two monoclonal antibodies that were used internationally.
●Involved in the work of one abstract and two scientific papers (Abstract 5 and Publications 1 & 2).
Department of Psychiatry, Division of Neurological Sciences (1990)
Mayo Clinic, Rochester, MN
Senior Research Fellow
Department of Neurology, Neurophysiology Laboratory (1988–1989)
●Involved in the work for two scientific papers (Publications 3 & 4).
Research Fellow
Department of Neurology, Neuroimmunology Laboratory (1985–1987)
●Involved in the work for four scientific papers (Publications 5, 6, 7, & 8).
Nanjing Railway Medical College, Nanjing, Jiangsu Province, China
Physician-in-Charge
Department of Neurology (1982–1985)
●Actively involved in teaching, clinical practice and research.
EDUCATION
Medical Doctor Degree - Graduated from Zhejiang University, School of Medicine, Hangzhou, Zhejiang Province, China.
TRAINING
Research Training Certificate - Department of Immunology & Neurology, Mayo Clinic, Rochester, MN
PROFESSIONAL MEMBERSHIPS
Member of the Mayo Alumni Association
Member of Chinese Medical Association Zhejiang Province and Jiangsu Province Branch
ABSTRACTS
1.Sisouvanthong, C., D. Ernst, L. Li, X. Wang, J. Chen, J.-P. Shih, J. Z. Huang, and G.-J. Gao. 2008. Regulatory T Cells and Mechanisms of Tolerance Induction: Multiparameter flow cytometric analysis of phosphorylated cell signaling proteins expressedby regulatory T cells (Tregs). FASEB J 22:848.31.
2.Sisouvanthong, C., D. Ernst, L. Li, X. Wang, J. Chen, J.-P. Shih, J. Z. Huang, and G.-J. Gao. 2008. Regulation of Signal Transduction in T and NK Cells: High-Throughput Evaluation of Janus Kinase-STAT and MARK Pathways in Normal Peripheral Whole Blood Cells using 96 Deep Well Plates by Flow Cytometry. FASEB J 22:1064.17.
3.Jing Ping Shih, Brad Paulsen, Naomi Nobida, Efthalia Chronopoulou, Xiao Wang, Guo-Jian Gao, Zhongxian Huang, Bing-Yuan Wei, Xiang Dong Ji and Roberto Campos-Gonzalez. Simultaneous use of Total and Phospho-specific Antibodies by Flow Cytometry to Study Cell Signaling Events. 2007. 1st National Congress on Signal Transduction, Mexican Society of Biochemistry, Veracruz, Mexico, L52.
4.Deming Sun, Jia-Yang Chen, Hui Shao, Joseph Huang, Ai-Li Wei, Henry Kaplan, and Bing-Yuan Wei. Cross-Species Interaction Between NKT Cels and the Recombinant Mouse/Human CD1d Dimers Complexed with α-galactosylceramide. (Abstract #1718)
5.T.J. Kiffer, R.A. Pederson, Z. Huang. Immunoreactive-GIP Release from a Tumor Derived Cell Line. The 5th International Symposium on VIP and Related Peptides. Nov. 12-15, 1991. Shizuoka, Japan.
PUBLICATIONS
1.T.J. Kiffer, Z. Huang, C.H.S. McIntosh, A.M.J. Buchan, J.C. Brown, R.A. Pederson. Gastric Inhibitory Polypeptide Release from a Tumor-Derived Cell Line (STC6-14). Am. J. Physiol. 269:E316–E322, 1995.
2.T.J. Kiffer, C.B. Verchere, C.D. Fell, Z. Huang, J.C. Brown. Glucose-Dependent Insulinotropic Polypeptide Stimulated Insulin Release from a Tumor-Derived β-Cell Line (β-TC3). Canadian Journal of Physiology & Pharmacology. 71:917–922, 1993.
3.D.W. Zochodne, Z. Huang, K.K. Ward, P.A. Low. Guanethidine-Induced Adrenergic Sympathectomy Augments Endoneurial Perfusion and Lowers Endoneurial Microvascular Resistance. Brain Research 519:112-117, 1990.
4.D.W. Zochodne, Z. Huang, K.K. Ward, P.A. Low. Enhanced Endoneurial Perfusion following Adrenergic Sympathectomy. Canadian Journal of Neurological Sciences. 16(2) P. 236, 1989.
5.V.A. Lennon, Z. Huang, D.J. Mccormick, G.E. Griesman, E.H. Lambert. Synthetic Peptide of Human Acetylcholine Receptor α-Subunit Sequence125–147 (Methionine 144) is a More Potent Autoantigen than its Norleucine 144 Analog. Ann. N.Y. Acad. Sci. 540:516–519, 1988.
6.D.J. Mccormic, G.E. Grismann, Z. Huang, E.H. Lambert, V.A. Lennon. Myasthenogenicity of Human Acetylcholine Receptor Synthetic α-Subunit Peptide 125–147 does not Require Intramolecular Disulfide Cyclization. The Journal of Immunology. 139:2615–2619, 1987.
7.V.A. Lennon, G.A. Griesmenn, D.J. Mccormick, Z. Huang, H. Feng, E.H. Lambert. Definition of Myasthenogenic Sites of the Human Acetylocholine Receptorusing Synthetic Peptides. Ann. N.Y. Acad. Sci. 505:439–450, 1987.
8.D.J. Mccormic, G.E. Grismann, Z. Huang, V.A. Lennon. Synthetic α-Subunit Peptide 125-147 of Human Nicotinic Acetylcholine Receptor Induces Antibodies to Native Receptor. FED. PROC. 45:919, 1986.
9.Z. Huang. The Late-Onset Epilepsy on CT Scan. The Medical Reference of Nanjing Railway Medical College, 1985.
10.Z. Huang & J. Hu. Intracranial Hypotension Syndrome Associated with Bilateral Subdural Haematoma. One Case Report. The Journal of Jiangsu Medicine, 1985.
11.Z. Huang. The Quantitative Determination of Enolase in Cerebral Spinal Fluid on Stroke. The Medical Reference of Nanjing Railway Medical College, 1985.
12.Z. Huang. The Epidemic Studying of the Relationship Between the Multiple Sclerosis and the Herpes Zoster. The Medical Reference of Nanjing Railway Medical College, 1984.
13.Z. Huang. The Clinical Investigation of the Anti-Carcinomatous Antibiotics B599. The Compilation of Shanghai Pharmaceutics Research Institute, 1972.
EXPERTISE & SPECIAL SKILLS
●SDS-PAGE
●Western blotting
●Autoradiography
●ELISA (Sandwich)
●Biotin Conjugation
●Microsurgery Technique
●Tissue Section Technique
●Lymphocyte Proliferation Assay
●Rat Heart Myocyte Isolation Method
●Cell Culture & Maintain Cell Lines
●Microcomputer Imagine Device System
●Flow Cytometry & Intracellular Staining
●Radioactive Immuno-Precipitation Assay
●Protein Purification (column chromatography)
●Cell Signaling Pathway Analysis (by Phosflow)
●Animal Immunization to generate antibody (Mouse, Rat, Rabbit)
●Cell Fusing (Hybridoma) and Production of Monoclonal Antibody
●Computer Software: Microsoft Word, Microsoft Excel, Power Point, Adobe Photoshop, FlowJo and CellQuest Flow Cytometry Analysis Softwares
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