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RÉSUMÉ

RONÉL KEEVÉ

Cell: 516-***-****

E-mail: ac0hk7@r.postjobfree.com

2

Personal details:

Ronél Keevé (ID no. 690-***-**** 08 0) was born on 10 July 1969. Nationality is South African. My address is 366 Macdonough street Brooklyn NY 11233. Education:

Institution attended Qualification obtained Year

Vryheid High School Matric* 1987

University of Pretoria BSc degree 1991

University of Pretoria

BSc (Honours) degree

(Biochemistry)

1993

Potchefstroom University

(University of the North West)

MSc degree

(Pharmaceutical Chemistry)

1997

* distinction in Physical Science

Recent courses, certificates and trainings completed:

Applied BioSystems basic training on LC-MS/MS, 2008

Lab House GC training course, 2008

Users’ meeting, Applied BioSystems, 2009

Industrial Analytical sample analysis and preparation by ICP, 2009

Users’ meeting, ABsciex, 2010 (Bloemfontein)

SANAS 17025 Laboratory Systems course, 2010

SANAS 17025 Internal Auditing course, 2010

Statistics and Chemometrics for Analytical Chemistry, 2010, University of Pretoria (internationally examined)

Instrumental analytical techniques, 2011, University of Pretoria (internationally examined)

Advanced triple quadTM LC-MS/MS training, ABSciex, attended in Darmstadt, Germany, 2012

Statistical Method Validation in Analytical Chemistry, 2012, Vision Enlight

Calibration and Regression Analysis for Analytical Chemists, 2013, Vision Enlight

SANAS 17025 Document Your System course, 2013

Registered at SACNASP as a Professional Natural Scientist, 2012 - current

International Certificate of Christian Education (ICCE) Internal Moderation course and certificate. Grade 2-12 at Robertson, April 2015.

Accelerated Education Training Certificate for completing a 5 day training course on the procedures and methodology of the A.C.E. programme 3 July, 2015.

Work experience:

Position held: Medical Technician

Company: Dr Du Buisson and Partners

Duration: 1989 - 1994 (full-time, part-time while studying) 3

Duties: Analysis of blood samples in biochemistry using automated blood analysers e.g. electrolytes, liver function, glucose, cholesterol, uric acid. Analysis of blood samples for lithium, zinc and iron using atomic absorption. Electrophoresis of blood samples.

Position held: Laboratory Assistant

Company: Potchefstroom University

Duration: 1994 - 1995

Duties: Responsible for primate project. Preparation of sterile (HPTP) solutions to be injected in baboons. Synthesis of metabolites of haloperidol for confirmation in metabolic studies on GC-MS. Preparation of baboon urine extracts for metabolic studies on GC-MS.

Position held: Quality Controller

Company: Pharma Natura

Duration: 1997 - 1998

Duties: Analysis of tablets using HPLC for active ingredients (vitamins). Analysis of raw material for quality control.

Position held: Laboratory Manager

Company: Onderstepoort Veterinary Institute

Duration: 1998 - 1999

Duties: Preparation of laboratory for accreditation. Wrote SOP’s. Developed methods for veterinary drugs (tetracyclines and -lactams) in kidneys using HPLC and SPE. Analysis of bovine, porcine and ovine kidney samples for these drugs using HPLC (PDA) detection. Training of staff to implement the methods.

Position held: Technical Specialist

Company: CSIR

Duration: 1999 - 2004

Duties: Analysis of samples for organic and biochemical production systems using GC (FID), HPLC (PDA, electrochemical amperometry and fluorescence detectors) and ion chromatography (electrochemical detector, conductivity mode using regenerating suppressors). Preparation of laboratory for accreditation. Preparation of environmental samples (water and ground) for pesticides (basic and acidic) to be analysed on GC-MS to comply with accreditation. Method development for PAH’s in environmental (water) samples using HPLC

(fluorescence and UV). Method development on evaporative light scattering detector. Method development and validation of amnesic shellfish poisoning in shellfish (domoic acid) using HPLC (PDA) and SPE according to SANAS ISO 17025. Analysis of proteins in food samples using kjeldahl method, total fats using acid hydrolysis and soxhlet extraction, and crude fibre in pet food. Verification of fat acid hydrolysis method and crude fibre method to comply with SANAS 17025 accreditation. SANAS technical signatory for biotoxins 4

Position held: Principal Forensic Analyst

Company: Department of Health, Forensic Chemistry Laboratory Duration: 2004 - 2011

Duties: Method development, validation and analysis of food samples to comply with Foodstuffs, Cosmetics and Disinfectants Act and also ISO 17025. Using HPLC (PDA and fluorescence) for preservatives (benzoic acid, sorbic acid) and caffeine in yoghurts, biscuits, sauces, soups, broths, soft drinks and dried fruit; vitamins (vitamin A palmitate, riboflavin) in bread flour and maize meal; water-soluble colours (13 azodyes) in soft drinks, sauces, sweets, extruded snacks, potato snacks, soups, broths, biscuits and oil-soluble colours (10) in spices and sauces using SPE. General maintenance was also done e.g. changing frit, needle seat and needle on HPLC. Also using LC-MS/MS in ESI, both positive and negative ionization modes: method development, validation and analysis of melamine in milk products (milk, milk powders, yoghurts, biscuits, chocolates and sweets) using SPE; antibiotics (40) in honey and eggs using SPE; mycotoxins

(patulin) in apple juice, deoxinivalenol in flour and maize meal, ocratoxin A and B in coffee and dried fruit, and aflatoxins G1, G2, B1 and B2 in peanuts and peanut butter using Romer MycoSep and Vicam (immunoaffinity) cartridges for sample clean-up; oil-soluble colours (screening) and quantification of Fast Garnet, Orange II and Rhodamin B in spices and sauces; pesticides (130) in fruit, fruit juices, vegetables, dried fruit, wine, wheat, barley and oats using Quechers sample clean-up, including tuning for these analytes; cleaning of LC-MS/MS (Q zero) and changing capillary when needed. In addition also ELISA, for aflatoxins (total) in peanuts, peanut butter, wheat, rice, cereals and chloramphenicol in honey and eggs. Training of staff to implement these methods, making sure correct method procedures are followed during sample preparation according to ISO 17025. Checking of chromatograms and calculations of junior analysts. Checking of labels to see if they comply with Foodstuffs, Cosmetics and Disinfectants Act. Typing of reports in MS Word. Analysis of blood alcohol samples using GC headspace. Method development and analysis of pesticides in post-mortem blood, stomach, liver and kidney using LC- MS/MS.

Position held: Technical Specialist

Company: CSIR

Duration: 2011 - 2013

Duties: Method development and validation of lipophilic shellfish poisoning toxins (DSP) on LC-MS/MS in mussels, oysters and abalone. Method development and validation on LC-MS/MS of all nine B vitamins. Routine monitoring and analysis of these analytes. Both validations complied with SANAS ISO 17025 accreditation criteria. Method development of paralytic shellfish poisoning (PSP) toxins on HPLC (fluorescence detection). Writing the standard operating procedures and the calculation of the uncertainty according to SANAS 17025 guidelines. Position held: Technical Specialist (Director)

Company: Unity Laboratory Solutions

Duration: 2013 - current

Duties: Be responsible for the technical side of the laboratory. Visiting prospective clients together with the Laboratory Manager and assisting with technical advice to the clients.

5

Part Time Position

Position held: Supervisor and Internal Moderator for Grade 2-12 School: Rabboni Christian Education Centre

Duration: 2014 -2015

Duties: Be responsible for the supervision of the grade 2-5 classes. Include assistance in academic work, marking and moderation of PACEs and writing of quarterly and annual reports. Be responsible for the internal moderation of grade 2-12 PACEs according to ICCE PACE Test Marking Criteria specific to South Africa and also international. Instrumental techniques recently used:

HPLC-FLD/DAD/UV

LC-MS/MS

ELISA

ICP

GC and GC (headspace)

Sample preparation techniques:

SPE

Quechers

Romer

Immunoaffinity

Computer programs and software recently used:

ChemStation

Analyst 5.1

MS Word 2007

Excel 2007

LIMS

Publications:

1. Haloperidol and its tetrahydropyridine derivative (HPTP) are metabolized to potentially neurotoxic pyridinium species in the baboon. Kathryn M Avent, Etsuko Usuki, Darryl W. Eyles, Ronel Keeve, Cornelis J. Van der Schyf, Neal Castagnoli, Jr and Susan M. Pond. Life Sciences, Vol 59, No 17, pp 1473-1482, 1996

2. Effect of the Haloperidol Tetrahydropyridine Metabolite 4-(4-Chlorophenyl)-1-[4-

(4-fluorophenyl)-4-oxobutyl]-1,2,3,6-tetrahydropyridine on Dopamine Receptor and Transporter Binding. A nonhuman primate 123I-iodobenzamide and 2 - carbomethoxy-3 -(4-iodophenyl)tropane single photon emission computed tomographic study. Douglas W. Oliver, Irene C. Dormehl, Cornelis J. Van der Schyf, John L. Neumeyer, Niek Hugo, Ronel Keeve, Neil T. Rossouw, Hans- 6

Wilhelm Müller-Gärtner and Neal Castagnoli, Jr. Arzneimittel-Forschung/Drug Research 47(1), 6, 692-699, 1997

3. Long-term treatment with the tetrahydropyridine analogue (HPTP) of haloperidol influences dopamine ligand binding in baboon brain. An [123I]iodobenzamide

(IBZM) SPECT study. Cornelis J. Van der Schyf, Irene C. Dormehl, Douglas W. Oliver, Nicolaas Hugo, Ronel Keeve, H.-W.Müller-Gärtner, Susan M. Pond, Neal Castagnoli Jr. Molecular Brain Research 43 (1996) 251-258 Posters:

1.) Method Validation and Routine Monitoring of Lipophilic Toxins in Shellfish in Southern Africa by LC-MS/MS. Keevé R, Dyan T, Foord C J, Mngxekeza T, Mvalo N and Simmers T D M. 4th Joint Symposium and AOAC Task Force Meeting, Marine and Freshwater Toxins Analysis. Organizers: AOAC International and EU Reference Laboratory, Marine Biotoxins, University of Vigo, Spain, 2013.

2.) Determination of Vitamin B12 in Fortified Food by LC-MS/MS. Ronél Keevé, Theresa Simmers, Chloë Laubcher, and Matthew Smith. Unity Laboratory Solutions (Pty) Ltd, 24 Kunene Street, Kuils River, Cape Town, South Africa, 7580 and Rabboni Christian Education Centre, 3 Musante Close, Saxenburg Park, Kuils River, Cape Town, South Africa, 7580. May-June 2015. Achievements:

1.) Sunday Times, 20 March 2005, Food cancer scandal. Did analysis for Sunday Times on food products, which caused national recall of foods contaminated with Sudan Dye 1 and 4.

2.) Testified in Cape Town Magistrate’s Court on work done on LC-MS/MS, 2012. 3.) Assisted with EPAP labelling by developing analytical methods and analysing all nine B vitamins on LC-MS/MS, 2012.

Hobbies

1.) Touring.

2.) Gardening.

3.) Hiking trips in nature

References:

1. André Baard, +272*-***-****

2. Annetjie Schillack, +272*-***-****

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