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Location:
Washington, DC
Posted:
May 21, 2017

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Resume:

Ayelen Bulloj

**** ********* **. ** # ***, Washington, DC 20009 202-***-**** ac0e60@r.postjobfree.com

www.linkedin.com/in/ayelen-bulloj-61a88a4

Professional Overview

Seasoned biologist with 16 years in laboratory research, specializing in molecular and cellular biology with extensive knowledge of retinal and neurodegenerative diseases. Conversant bilingual English/Spanish.

Experience

Postdoctoral Fellow 2009-present

Fordham University Bronx, NY, USA

Conduct research in molecular mechanisms underlying age-related macular degeneration (AMD) with special emphasis in photoreceptor phagocytosis by retinal pigment epithelial cells (RPE)

Identified and characterized novel proteins and signaling pathways that affect phagocytosis by RPE cells in primary cell culture and different animal models

Development and maintenance of primary and commercial retinal pigment epithelial cells (cell culture)

Experimental design and execution of electroretinogram for mice and rats

Expertise in preparation and sectioning of embedded tissues in paraffin and OCT (microtomy, cryotomy)

Design experimental planning, execution and trouble-shooting in ELISA, western blotting, immunohistochemistry (IHC) and immunocytochemistry (ICC).

Planning and maintenance of transgenic mouse and rat colonies, including genotypic analysis by PCR

Published two (2) scientific publications and one (1) chapter in book related to neurodegenerative eye diseases

Supervised undergraduate students and technicians in designing, performing and trouble-shooting experiments

Ph.D. Candidate 2003-2009 Fundacion Instituto Leloir

Buenos Aires, Argentina

Focused in membrane association, trafficking and secretion of Insulin Degrading Enzyme, a key protease involved in the metabolism of insulin and amyloid ß in Alzheimer`s disease

Isolated and purified exosomes and lipid rafts using discontinued sucrose gradients

Prepared primary cell culture of neurons and astrocytes. Maintenance of commercial mammalian cell lines.

Performed gene cloning, PCR/RT-PCR/qPCR, gel electrophoresis, protein expression and purification (E.coli and mammalian cells), microbiological techniques, western blotting, immunoprecipitation, immunohistochemistry, confocal microscopy; Handled radioactive material for radiolabeling

Published four (4) scientific reports in international journals and 1 chapter in textbook related to Alzheimer disease; presented research work in six (6) international scientific meetings

Internship 2004-2005

Center for Neuroscience and Aging, The Burnham Institute San Diego, CA, USA

Undergraduate Student 2000-2003

Fundacion Instituto Leloir Buenos Aires, Argentina

Culture of mammalian cell lines (human, rat, mouse)

Preparation of media and reagents for cell culture. Preparation of solutions and laboratory material

Immunofluorescence and confocal microscopy

Co-author in poster presentation at 4 international scientific meetings

Education

Ph.D in Biological Chemistry 2009

University of Buenos Aires; School of Natural and Exact Sciences Buenos Aires, Argentina

Master of Science: Molecular Biology and Biotechnology 2003

University of Buenos Aires; School of Natural and Exact Sciences Buenos Aires, Argentina

Related coursework:

Flow Cytometry: Principles and Methods

2017

National Institutes of Health (NIH)

Bethesda, MD, USA

Genotoxicity workshop. Development and Risk assessment

2017

Bioreliance

Rockville, MD, USA

Handling of laboratory animals

2008

University of Buenos Aires Buenos Aires, Argentina

Experimental Design and Determination of Sample Size in work with laboratory animals

2008

University of Buenos Aires Buenos Aires, Argentina

EMBO Practical Course: "Advances in Technologies for High Resolution in Vivo Microscopy”

2006 Buenos Aires, Argentina

Public Speaking and Lecture Methods

2003

University of Buenos Aires. Centro Cultural Ricardo Rojas, Argentina

Publications

Semaphorin4D-PlexinB1 Signaling Attenuates Photoreceptor Outer Segment Phagocytosis by Reducing Rac1 Activity of RPE Cells

Mol Neurobiol. 2017 Manuscript Accepted.

PI 3-kinase independent role for AKT in F-actin regulation during outer segment phagocytosis by RPE cells

Exp Eye Res. 2013 Aug;113:9-18

Insulin-degrading enzyme sorting in exosomes: a secretory pathway for a key brain amyloid-beta degrading protease

J Alzheimers Dis. 2010 Jan;19(1):79-95.

Detergent resistant membrane-associated IDE in brain tissue and cultured cells: Relevance to Abeta and insulin degradation

Mol Neurodegener. 2008 Dec 31;3:22.

Tumor suppressor PTEN affects tau phosphorylation: deficiency in the phosphatase activity of PTEN increases aggregation of an FTDP-17 mutant Tau

Mol Neurodegener. 2006 Jul 31;1:7

Tumor Suppressor PTEN Affects Tau Phosphorylation, Aggregation and Binding to Microtubules

FASEB J. 2006 Jun;20(8):1272-4

Multiple Display of a Protein Domain on a Bacterial Polymeric Scaffold

Proteins. 2005 Dec 1;61(4):1089-100

Amyloid Beta Degradation: a Challenging Task for Brain Peptidases

Subcell Biochem. 2005;38:129-45. Review

Contributing Author, Textbooks

Vision and Grapes

Grapes and Health 2015 Dec; pp.213-235

Amyloid -degradation: A challenging task for brain peptidases

In Alzheimer’s disease: Cellular and Molecular Aspects of Amyloid by J.Robin Harris and Falk Fahrenhols. Kluwer Academic/Plenum Publishers. 2004

Skills

RT-PCR/PCR/qPCR

DNA/RNA purification/ isolation

Primer design

Protein expression and purification (E.coli and mammalian)

Microbiological techniques

Biosafety Level 2 (BSL-2) techniques

Primary cell culture preparation

Western blotting

ELISA

Continuous and discontinuous gradients

Immunofluorescence assays (IHC, ICC)

Preparation and Sectioning of embedded tissues in paraffin and OCT

Confocal microscopy

Flow cytometry

Electroretinography (ERG)

Animal handling

Solid oral and written communication skills

Proficient in Excel, PowerPoint, Word applications, Graphpad Prism and Photoshop

Conversant bilingual English/Spanish



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