Bioinformatics, Biotechnology, Molecular Biology

HARSH DESAI

* ********* ***, ******, ** ***** 857-***-**** *****.*@*****.***.*** www.linkedin.com/in/hddesai EDUCATION

Northeastern University, Boston, MA Jan-2018 (Anticipated) College of Science

Candidate for a Master of Science in Bioinformatics Related Courses: Bioinformatics Computational Methods, Molecular Biology, Statistics for Bioinformatics, Collecting Storing and Retrieving data, Bioinformatics Programming

Sardar Patel University, Vallabh Vidyanagar, India Master of Science in Environmental Science and Technology July 2013-June 2015 Bachelor of Science in Biotechnology June 2010-April 2013 Related Courses: Application of Biotechnology, Biochemistry, Immunology, Cell and Molecular Biology SKILLS

Programming Languages: Perl, R Programming, Python, Shell scripting, NoSQL(mongolite), SQLite

Bioinformatics Tools: BioPerl modules, Trinity, Bowtie2, DEseq2, BLAST, FASTA, ClustalW, BCF-VCF Tools, SOAPDenovo2, ALLPATHS-LG, MUMmer, MAFFT, Moose, Eclipse, Galaxy, Geneious

Analytical Lab Techniques: SDS-PAGE, Agarose gel Electrophoresis, UV-VIS Spectroscopy, HPLC, GC-MS, Western Blotting, Southern Blotting, ELISA, PCR, DNA/RNA Microarray, Environmental analysis techniques WORK EXPERIENCE

Northeastern University, Boston, MA August 2016-Present Residential Security Office Proctor

Maintain access control and safety in the residence halls of Northeastern University

Serve as the first University contact for non-resident visitors and campus information

Effectively manage, document and follow up with any security issues that may arise Enviro Technologies (Environment Consultant), Vadodara, India June 2015-Nov 2015 Assistant Executive

Samples like effluent treatment water, soil sample collection and analysis from various industrial sites

Providing guidance to various industries for maintaining water and hazardous waste pollution under prescribed limits

Preparation and updating SOP, troubleshooting and overall Lab management Concept Biotech Ltd, Vadodara, India January 2013-July 2013 Project Trainee

Providing On-site and Off-site assistance for project pipeline

Preparation of chemical reagents and glassware and provide help in overall lab management

Perform bacterial DNA isolation, PCR and Agarose gel electrophoresis for strain identification

Literature review, presentation and report preparation and helping in Client handling and management ACADEMIC PROJECTS

Perl:

• CRISPR target search of Drosophila genome using Perl programming and Bio-perl modules

• Denovo assembly of Bacterial genome(Rhodobacter) using ALLPATHS-LG and SOAPDenovo2 assemblers.

• Transcriptome assembly from RNA-Seq illumina reads using Trinity assembler and Identification of differentially expressed isoforms and genes using Trinity pipeline

• Short reads alignment of Human-X chromosome using Bowtie-2 and Samtools

• Multiple Sequence Alignment using Clustal Omega and MAFFT

• Building phylogenetic trees from reads using maximum-likelihood based program RaxML

• Whole genome alignment of Helicobacter pyroli strains using MUMmer, NUCmer and Promer

• Browsing and fetching gene information and sequence data information from gene data portals like NCBI, Pfam, Uniprot

R Programming:

• Conduct hypothesis tests one sample t-test, two sample t-test and binomial test

• Use of Monte Carlo simulations to check various probabilities of random variable

• Applying different classification methods like logistics regression, support vector machine and tree for classification

• Gene filtering, annotation, ANOVA analysis and application of linear model using Bioconductor

• K-means and Hierarchical clustering and distance measure on Iris dataset

Effect of Various Disinfectants On E. coli by Phenol Coefficient Method as a part of Bachelor’s program. In this project various Disinfectants like Colgate Plax, Phenyl, Dettol and Phenol (standard disinfectant) were selected on the basis of their specificity against microbes to test their effects on the growth of various bacteria by Phenol coefficient method. With this method, potency of a disinfectant against different microbes was determined.

Dissertation on Determination of physio-chemical properties of Municipal solid waste at Anand MSW dumping station, Gujarat, India.

In this project, solid waste and soil sample were collected from Anand MSW dumping site on biweekly bases for three months and analyzed for various physio chemical parameters like Density, TOC, TOM, sulphate, phosphate, Iron, chromium. Final status report was prepared and submitted to authorities.

Characterization of Diastase Enzyme In which, Diastase enzyme samples from different manufacturers were tested for enzyme activity under multiple conditions using DNS method and final comparative report was prepared.

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