Scientist, microbiologist, cell/molecular biologist, genomics

ANA M. GUZMAN PRIETO

Dynamic, critical and organized Microbiologist with a Ph.D. degree. Currently, seeking for a challenging position, in which I can help propel the interests of the company, contribute to meaningful research and advance my career.

236 Entrance Road, Apt 8, CA93117, Santa Barbara, California (USA) ***.******.******@*****.*** +1-805-***-****

www.linkedin.com/in/AnaGuzmanPrieto

EDUCATION

Ph.D. Functional Genomics Utrecht University Utrecht, The Netherlands (NL) 2017 Bio-Business Program Summer School Utrecht Utrecht, NL 2014 M.Sc. Cell and Molecular Biology Utrecht University 2011 Hubrecht Institute, Utrecht Rabouille group 2010 UMC Utrecht Dep. of Cell Biology 2009

B.S. Microbiology Universidad del Valle Cali, Colombia 2006 AWARDS

Utrecht University Excellence Scholarship - fully funding master studies 2009-2011 Universidad del Valle Excellence Scholarship - partially funding bachelor studies 2003-2006 AFS-Intercultural Programs Scholarship - fully funding foreign exchange studies in USA 1999-2000 PROFESIONAL EXPERIENCE

University Medical Center Utrecht (UMCU) 2011 - 2017 Department of Medical Microbiology

Researcher

* Responsible for designing and executing experiments to answer particular research questions.

* Interpreted independently laboratory results and analyzed next generation sequencing data.

* Discussed scientific data locally and at international conferences.

* Published manuscripts in peer-reviewed international journals. Project:

Mechanisms of colonization, virulence and antibiotic resistance in Enterococcus faecium. Techniques used: construction of deletion mutants and in trans complementation, transcriptome analysis, qRT-PCR, 5’RACE, protein purification, flow cytometry, ELISA, ligand affinity blotting, antibody production and labeling, fluorescent microscopy, microarray hybridization, scanning electron microscopy (SEM), RNA sequencing (RNA-seq), bioinformatics, generation of transposon mutant libraries, transposon sequencing (Tn-seq), web-based platforms to analyze next generation sequencing

(NGS) data, genome sequencing, antimicrobial susceptibility testing, allelic overexpression. EvoTAR 2011 - 2016

European consortium for the Evolution and Transfer of Antibiotic Resistance Project manager

- 4 years of experience in scientific project management for a large European consortium including 17 institutions in 6 countries.

- Responsible for planning and execution of 4+ projects of the Dutch branch carried at the UMCU.

- Contributed to maintain projects on schedule and in line with shareholders expectations.

- Responsible for delicate and complex laboratory equipment.

- Managed laboratory inventory, ensuring proper availability of laboratory supplies and reagents.

- Monitored proper handling of equipment following good laboratory practices (GLP).

- Ensured quality control and took preventive and corrective actions.

- Managed and analyzed large NGS data.

- Trained and supervised internal staff for the different projects.

- Supported and motivated internal team members.

- Communicated advances in different projects to scientific members of the consortia and resolved uncertainties during annual international progress meetings.

- Accomplished to create relationships and collaborations with third parties when required to full fill a particular goal.

University of Texas Medical School 2008

Division of Infectious Diseases

Visiting scientist

- Learned bioinformatics and molecular techniques abroad, in order to train national technicians and students.

International Center for Medical Research and Training (CIDEIM) 2006 - 2008 Antibiotic resistance line

Research assistant

- Identified circulating antibiotic resistance mechanisms in clinical isolates of local hospitals, using diverse molecular techniques, such as polymerase chain reaction (PCR), isoelectric focusing

(IEF) and pulse field gel electrophoresis (PFGE), towards the identification

- Wrote standard operating procedures (SOP).

- Analyzed scientific data analysis and discussed results with team members.

- Prepared scientific manuscripts for publishing.

International Center for Medical Research and Training (CIDEIM) 2005 - 2006 Diagnostics department

Clinical laboratory scientist

- Responsible for immunodiagnosis tests for leishmaniasis.

- Responsible for administering and evaluating the Mantoux tuberculin skin test in outpatients.

- Collected clinical and environmental samples for microbiological analysis.

- Cultured and identified clinical isolates using diverse culture media and the VITEK® 2 system.

- Performed antibiotic susceptibility testing by micro-both dilution (MIC) method of identified bacterial isolates.

SUMMARY OF QUALIFICATIONS

- Self-motivated, resourceful and able to motivate others.

- Strong leader with excellent communication and interpersonal skills.

- Solid analytic thinking.

- Compliance with established procedures.

- Organized and effective to meet required deadlines.

- Fast and dedicated apprentice when challenged with new knowledge.

- Fluent spoken Spanish (native), English and Italian. PUBLICATIONS

- Guzman Prieto AM, et al. Antimicrobial Agents and Chemotherapy. 2017;24;61(5). pii: e02122- 16. doi: 10.1128/AAC.02122-16.

- Zhang X & Guzman Prieto AM, et al. Preprint in BioRxiv. 2017. BioRxiv 101329; doi: https://doi.org/10.1101/101329.

- Guzman Prieto AM, et al. Frontiers in Microbiology. 2016;26(7)788. doi: 10.3389/fmicb.2016.00788

- Niebel M, et al. International Journal of Antimicrobial Agents. 2015;46(5):572-5. doi: 10.1016/j.ijantimicag.2015.07.009.

- Guzman Prieto AM, et al. Nature Scientific Reports. 2015;17(5):18255. doi: 10.1038/srep18255.

- Villegas MV, et al. Antimicrobial Agents and Chemotherapy. 2007;51(6):2001- 4. doi:10.1128/AAC.00226-07

- Villegas MV, et al. Antimicrobial Agents and Chemotherapy. 2007;51(4):1553-1555. doi:10.1128/AAC.01405-06.

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