Project University

MAROOF HUSAIN

HOME ADDRESS: **** * ****** *** ******, CO 80015, USA,

E-mail: *******@*****.***, Phone: 720-***-****

EDUCATION

****-**** **.*. ************, Aligarh Muslim University, Aligarh, India.

Thesis title: Studies on the mechanism of modulation of platelet

neurotransmitter receptors and their validation as neuronal model in

neurotoxicological disorders”

1994-1996 M.Sc. Biochemistry, Aligarh Muslim University, Aligarh, India.

1991-1994 B.Sc. Biochemistry, Aligarh Muslim University, Aligarh, India.

PROFESSIONAL EXPERIENCE

2004-Current: Postdoc/Research Associate/Assistant Research Professor: Dept. of Microbiology,

University of Colorado-Denver.

Project: Analysis of intracellular host defenses in Salmonella pathogenesis.

Maintained and cultured microorganism (aerobic and anaerobic, microbiological growth, culture,

storage, BSL2).

Studied molecular biology of host defenses (nitric oxide and H2 O2) in Salmonella.

Created mutant strain of Salmonella and assessed their virulence in cell based assays, phagocytosis

assays, and in vivo animal models of infectious disease.

Devised methods to purify thiol oxidized proteins from bacteria by employing of thiol oxidation,

blocking and bioconjugation approach.

Managed and supervised protein purification for the lab.

Conducted protein functional studies by assaying protein-protein, protein-DNA interactions and in

vitro transcription.

Designed methods for the assay of nitric oxide, H2O2 and oxygen with polarigraphic probes in liquid

samples (bacterial cultures) using Free Radical Analyzer (WPI).

Performed peer and technical review of laboratory data. Writing/reviewing of analytical test

methods, SOP’s and research communications.

Trained staff scientists and graduate students in good laboratory practice (GLP) and maintained

accurate training records.

Plan, schedule, conduct, and coordinate ongoing research projects.

2002-2004: Visiting Research Associate: Dept. of Clinical Pharmacy, University of Colorado-Denver.

Evaluated synergistic bactericidal activity among antibiotics against multi-drug resistance clinical

isolates.

Bactericidal activity assay using time-kill experiments according to the NCCLS methods.

Antibiotic MICs determination by broth microdilution according to NCCLS methods

Developed thorough understanding of microbiology and microbiological techniques including MLT,

AET, EAP, bioburden

1997-2002: Ph.D Work: Division of Developmental Toxicology, CSIR-IITR Lucknow India.

Project: Peripheral markers of central nervous system diseases.

Studied the neurotoxicity of industrial chemicals: manganese and pesticides

Assessed neurotransmitter receptor binding kinetic analysis in blood platelets and in nerve endings.

Validated blood platelets as peripheral markers for central nervous system.

1

SCIENTIFIC AFFILIATIONS

American Society for Microbiology

American Society for Biochemistry and Molecular Biology

Sigma Xi Society

REVIEW/ REFEREE ACTIVITIES

Reviewed manuscripts communicated to the Journal of Innate Immunity, Journal of Veterinary

Diagnostics Investigation, Molecular Cancer Therapeutics

Served as a judge for poster presentation in University of Colorado Postdoctoral Research Day, May

14th 2010.

SKILLS

Microbiology/Recombinant DNA technology: Bacterial cultures, gene deletion, EBU plate screening

•

phage lysis, transcriptional fusions, transformation, P22 phase Salmonella transductions.

Fermentation: Natural and recombinant bacteria, production optimization, enzyme production

•

Bacterial physiology: Polarigraphic measure of oxygen, nitric oxide and hydrogen peroxide, bacterial

•

inner membrane isolation and spectroscopic analysis of cytochromes in ETC, experience with anaerobic

chamber, experience with bacterial growth on automated system, experience in free radical and nitric

oxide biology/chemistry.

Assay development: Mammalian expression systems, cell based assays, primary cell culture,

•

cryopreservation of cultures, in vitro assays, secretion assays. Infrared dye and fluorescent-based assays.

Biochemistry and molecular biology: Protein expression, protein turnover/metabolism, SDS-PAGE,

•

native gel electrophoresis, two-dimensional gel electrophoresis, western blotting, immunoprecipitation,

biotin switch, in vitro transcription assay, radioligand binding, spectrophotometry, ELISA, enzyme

assays, PCR, qPCR, reporter plasmid transfection, DNA protein binding using fluorescence

spectroscopy/anisotropy and EMSA, sample preparation for mass spectrometric analysis.

Cell biology: Mammalian cell culture (A549, Hela, J774, macrophage), stable cell line, transfection,

•

clone selection, high throughput cell culture, immunohistochemistry (IHC) and immunocytochemistry

(ICC), flow cytometry.

Protein purification: Ammonium sulfate precipitation, ultrafiltration (UF), dialysis, size exclusion

•

(SEC), AKTA, ion exchange (IEX) and affinity chromatography for membrane and soluble proteins.

Familiarity with LC-MS, MALDI-TOF MS, HIAC, MFI, FlowCAM.

Animal models: Mouse models of Salmonella pathogenesis, rat models of neurodegenerative disease,

•

Blood, liver, spleen, brain removal and dissection, Animal surgery.

Computer Software Skills: Serial cloner, Vector NTI® Software, ImageJ, Adobe Photoshop, Image

•

Lab Software (Bio-Rad), Graph pad Prism and MS Office (Word, Excel, Power Point)

PUBLICATIONS

1. Song M, Husain M, Jones-Carson, Liu L, Henard CA, Vazquez-Torres A. 2013. Low molecular

weight thiol dependent antioxidant and antinitrosative defences in Salmonella pathogenesis. Mol

Microbiol. 87(3): 609-22.

2. McCollister BD, Hoffman MG, Husain M, Vazquez-Torres A. 2011. Nitric oxide protects bacteria

from aminoglycoside by blocking the energy-dependent phases of drug uptake. Antimicrob Agents

Chemother 55(5): 2189-96

3. Husain M, Jones-Carson J, Song M, McCollister BD, Bourret TJ, Vazquez-Torres A. 2010. Redox

sensor SsrB Cys203 enhances Salmonella fitness against nitric oxide generated in the host immune

response to oral infection. Proc. Natl. Acad Sci USA 1 07(32): 14396-401.

2

4. Husain M, Shukla R, Dikshit M, Maheshwari PK, Nag D, Srimal RC, Seth PK, Khanna VK. 2009.

Altered platelet monoamine oxidase-B activity in idiopathic Parkinson’s disease. Neurochem. Res.

34(8): 1427-32

5. Husain M, Bourret TJ, McCollister BD, Jones-Carson J, Laughlin J, Vazquez-Torres A. 2008. Nitric

oxide evokes an adaptive response to oxidative stress by arresting respiration. J Biol. Chem. 283(12):

7682-9.

6. McCollister BD, Myers JT, Jones-Carson J, Husain M, Bourret TJ, Vazquez-Torres A. 2007. N2O3

enhances the nitrosative potential of IFN gamma-primed macrophage in response to Salmonella.

Immunobiology 212(9-10): 759-69.

7. McCollister BD, Myers JT, Husain M, Vazquez-Torres A. 2006. Hmp protects Salmonella SPI2

expression from NO rates generated during innate response of macrophages. Nitric Oxide 14(4): 17

8. Jung R, Husain M, Choi MK, Fish DN. 2004. Synergistic activities of moxifloxacin with pipercillin-

tazobactam or cefepime against Klebsiella pneumoniae, Enterobactor cloacae, and Acinetobactor

baumanii clinical isolates. Antimicrob. Agents Chemother.48 (3): 1055-7

9. Shukla R, Husain M, Tandon R, Khanna VK, Nag D, Dikshit M, Srimal RC, Seth PK. 2003. Platelet

3

H-ketansirin binding in tension-type headache. Headache 43(2): 103-8

10. Husain M, Khanna VK, Roy A, Tandon R, Pradeep S, Seth PK. 2001. Platelet dopamine receptors and

oxidative stress parameters as markers of manganese toxicity. Hum. Exp. Toxicol. 20 (12): 631-636.

11. Shukla R, Khanna VK, Pradeep S, Husain M, Tandon R, Nag D, Dikshit M, Srimal RC, Seth PK.

2001. Platelet 3 H-ketansirin binding in migraine. Cephalalgia 21(5): 567-72.

12. Husain M, Khanna VK, Seth PK. 2001. Platelet dopamine receptor alterations following striatal 6 -

hydroxydopamine lesion in rats. Ann. Neurosci. 8:31-32

13. Khanna VK, Husain M, Dalal RK, Agarwal AK, Dikshit M, Nag D, Srimal RC, Seth PK. 2001.

Usefulness of platelet 5-HT2A receptors, intracellular calcium and membrane fluidity assay in

monitoring of depression. Ann. Neurosci 8:15

14. Husain M, Khanna VK, Shukla R, Nag D, Dikshit M, Srimal RC, Seth PK. 2000. Alteration in platelet

3

H-Ketanserin binding in cases of migraine. Ann. Neurosci. 7:30-31

15. Khanna VK, Husain M, Roy A, Shukla R, Nag D, Dikshit M, Srimal RC, Seth PK. 2000. Alteration in

selected biochemical parameters in idiopathic Parkinson’s cases. Ann. Neurosci.7: 25

REFERENCES

Andres Vazquez-Torres, PhD., DVM, Professor, Department of Microbiology, School of Medicine,

University of Colorado at Denver, 12800 E 19th Ave, Aurora, CO 80010, Phone: 303-***-****, Email:

******.*******-******@********.***

Ashraf A. Khan, Ph.D.

Research Microbiologist, Division of Microbiology (HFT-250), National Center for Toxicological Research,

U. S. Food and Drug Administration, 3900 NCTR Road, Jefferson, Arkansas 72079, phone: 870-***-****,

fax: 870-***-****, Email: ******.****@***.***.***

Jerome Schaack. Ph.D, Associate Professor of Microbiology, Department of Microbiology, School of

Medicine, University of Colorado at Denver, 12800 E 19th Ave, Aurora, CO 80010, Phone: 303-***-****,

Email: *****.*******@********.***

Aleem Siddiqui, PhD. Professor, Department of Medicine, University of California, 9500 Gilman Drive, La

Jolla, CA 92093, Phone: 858-***-****, Fax: 858-***-****, Email: *********@****.***

3

Contact this candidate