Engineering Assistant

Location:

Somerville, MA

Posted:

February 13, 2013

Contact this candidate

Resume:

Daniel Hines

Email: *********@********.***

Address: * ******* **. *** **

City: Somerville

State: MA

Zip: 02144

Country: USA

Phone: 774-***-****

Skill Level: Entry

Salary Range: $100,000

Willing to Relocate

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

Daniel Joseph Hines Ph.D.

9 Holland St. Apt. 3B, Somerville, MA 02144

774-***-**** / ********@*****.***

EDUCATION

Jan. 2009 - May 2012 Tufts University

Doctor of Philosophy (PhD) in Chemical Engineering

* Dissertation Title: Characterization & Mathematical Modeling of Silk Based Drug Release Systems

* In vitro experimentation of silk fibroin based polymeric drug release devices for various device morphologies and releasing solutes (macromolecule, small molecule)

* Established kinetic parameter - material property relationships through mathematical modeling of release data via multivariate regression analysis and parameter estimation

* Developed a novel automated continuous flow release system with real time measurement to increase robustness of collected In vitro data and regression statistics

Sept. 2006 - Dec. 2008 Tufts University

Master of Science (MS) Chemical Engineering

* Thesis: Developing Patient Specific Population Pharmacokinetic Models and Comparing Compartmental vs. Non-compartmental Analysis for the Prediction of Individual Pharmacokinetic Parameters

* Compared compartmental and non-compartmental modeling approaches towards the estimation of individual PK parameters and individual dosage optimization

* Successfully applied a population PK analysis to reduce observed variability among predicted PK parameters and optimize dosage by accounting for patient factors

Sept. 2002 - May 2006 University of Massachusetts Amherst

Bachelor of Science (BS) Chemical Engineering

Sept. 2002 - May 2006 University of Massachusetts Amherst

Bachelor of Science (BS) Biochemistry and Molecular Biology

Sept. 1999 - May 2002 Bishop Stang High School

INDUSTRY EXPERIENCE

May 2008 - Sept. 2008 Sepracor Inc.

Engineering Associate, Research and Development

* Synthetic API production; Process optimization; Bench & kilo-scale; Analyzing and predicting scale-up/scale-down performance of all batch unit operations for GMP, GLP, SOP; Unit operations include reaction, mixing, heat/mass transfer, crystallization, filtration, drying.

* Development of robust crystallization procedures in terms of API polymorphic form and particle size control for late stage development programs. Analysis of crystal growth from empirical data collected for varied solvent concentration and cooling profiles.

* API stability testing and mathematical modeling of degradation kinetics. Determination of expiration period for various storage temperatures and registration of results with FDA. Use of advanced mathematical software to efficiently analyze complex data from laboratory or manufacturing operations and develop reliable statistical models.

ACADEMIC EXPERIENCE

Jan. 2009 - May 2012 Tufts Biomedical Engineering Department

Graduate Research Assistant

* Successfully led the development and optimization of silk fibroin based polymeric controlled/sustained release devices. Delivery device forms include: implantable films, injectable hydrogels & micro-/nano-particles, pellets, reservoirs devices, tissue engineering scaffolds, layered and complex designs.

* Led the design and development of a novel continuous flow In vitro dissolution apparatus with real time data acquisition for the collection of robust data sets for increased kinetic modeling accuracy; Release data collected for various solutes ranging from macromolecules (i.e. proteins) to small molecule.

* Coordinated several data analysis and modeling efforts within the research group on various tissue engineering and drug delivery projects (i.e. regression analysis, parameter estimation, statistics)

* Led the characterization of silk fibroin delivery device release mechanisms and kinetic parameter - material property relationships for use as prediction models of drug release.

* Successful characterization and optimization of regenerated silk fibroin polymer processing methods.

* Supervised the development of a polymeric support matrix for long term stabilization of biological macromolecules such as proteins, DNA, and RNA.

* Protein production and purification via transfected E. Coli culture and affinity chromatography.

* Successfully applied various analytical methods towards the characterization of polymeric device material properties.

* Effectively supervised 7 undergraduate research projects

Sept. 2006 - Jan. 2009 Tufts University Department of Chemical & Biological Engineering

Graduate Research Assistant

* Led the collaboration of the Tufts chemical engineering department and Tufts medical school for a population pharmacokinetic analysis for the development of patient specific pharmacokinetic models.

* Estimation of pharmacokinetic parameters via regression of blood concentration time profile data

* Dosage optimization and prediction of patient specific pharmacokinetic parameters through incorporation of individual patient factors (i.e. age, sex, weight)

Spring 2008 & 2009 Tufts University Department of Chemical & Biological Engineering

Graduate Teaching Assistant

* Served as teaching assistant and grader for chemical engineering chemical process design course

Fall 2008 Tufts University Department of Chemical & Biological Engineering

Graduate Teaching Assistant

* Served as teaching assistant and grader for chemical engineering process dynamics & control course

May 2005 - May 2006 University of Massachusetts Amherst Department of Chemical Engineering

Undergraduate Research Assistant

* Comparison of the effects of various culture media sterilization techniques on taxane production levels in Taxus plant cell culture; Quantification of substrate concentration variability in sterilized suspension culture media

* Comparing effects of elicitation techniques on Paclitaxel accumulation in Taxus plant cell culture, Cellular & metabolic engineering; Quantification of Taxane accumulation in cell culture via HPLC

* Comparing the effects of Taxane separation methods on HPLC interference

COMPUTATIONAL SKILLS

Microsoft Office, Excel, MATLAB, ASPEN, MathCAD, MINITAB, regression analysis, nonlinear regression, multivariate regression, parameter estimation, model building and fitting, statistics, numerical modeling, partial least squares

ANALYTICAL/LABORATORY SKILLS

UV-Vis Spectroscopy, SEM, Fluorescence, Confocal Microscopy, PCR, RT-PCR, Electrophoresis, FT-IR, ELISA, TGA, Chromatography (size exclusion, Ni NTA), LC/MS, HPLC, FBRM, Raman, Calorimetry, Automated batch process equipment (Labmax, Multimax), Bioreactors, Fermentation, Suspension cell culture, GLP, GMP, SOP

PUBLICATIONS

Hines, D.J., Kaplan D.L., (2011) Mechanisms of Controlled Release from Silk Fibroin Films, Biomacromolecules 12, 804-812

Hines, D.J., Kaplan D.L., (2013) Characterization of Small Molecule Controlled Release from Silk Films, Macromolecular Chemistry and Physics 280, 280-294

Hines, D.J., Kaplan D.L., (2012) Poly (lactic-co-glycolic acid) controlled release systems: experimental and modeling insights, Accepted

Gallego-Llamas, J., Hines, D. J., Kaplan D.L., (2012) Size effect of silk particles on the inflammatory response, Pending

ACCOMPLISHMENTS & AWARDS

* American Institute of Chemical Engineers Member (AIChE) Fall 2003 - Present

* 2008 AIChE Annual Meeting Speaker, Hines, D.J., Georgakis, C., Developing Patient Specific Pharmacokinetic Models: Population Pharmacokinetic Analysis of Triazolam, 2008 AIChE Conference Proceedings

* Tufts Graduate Student Travel Grant for Presentation at the Annual AIChE Meeting, 2008

* 2010 AIChE Annual Meeting Speaker, Hines, D.J., Kaplan D.L., Characterization and Modeling of Controlled Release From Silk Fibroin Based Drug Delivery Devices, 2010 AIChE Conference Proceedings

* 2011 AIChE Annual Meeting Speaker, Hines, D.J., Kaplan D.L., Diffusion Coefficient Prediction Model for Sustained Release From Silk Fibroin Films, 2011 AIChE Conference Proceedings

* Elected AIChE UMass Amherst Student Chapter Treasurer, May 2005 - May 2006

* International Society of Pharmaceutical Engineers (ISPE) Fall 2003 - Present

* Elected ISPE UMass Amherst Student Chapter Vice President, May 2005 - May 2006

* First Place Winner, Tufts Biomedical Engineering Department Art Contest, 2011

* First Place Winner, International Silk Art Olympics, 2011

REFERENCES

Dr. David Kaplan (PhD Adviser)

Tufts University Department of Biomedical Engineering Chair

4 Colby St., Medford, MA 02155

Tel: 617-***-****

Email: *****.******@*****.***

Dr. Kostas Saranteas (Industry Contact)